Experiment: PYE with Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02008 and CCNA_02009 overlap by 1 nucleotides CCNA_02009 and CCNA_02010 are separated by 18 nucleotides CCNA_02010 and CCNA_02011 overlap by 1 nucleotides CCNA_02011 and CCNA_02012 are separated by 108 nucleotides
CCNA_02008: CCNA_02008 - prolyl-tRNA synthetase, at 2,155,544 to 2,156,875
_02008
CCNA_02009: CCNA_02009 - hypothetical protein, at 2,156,875 to 2,157,291
_02009
CCNA_02010: CCNA_02010 - hypothetical protein, at 2,157,310 to 2,157,570
_02010
CCNA_02011: CCNA_02011 - methylmalonyl CoA epimerase, at 2,157,570 to 2,157,989
_02011
CCNA_02012: CCNA_02012 - metal-dependent hydrolase, at 2,158,098 to 2,159,777
_02012
Position (kb)
2157
2158 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2156.914 kb on - strand at 2156.925 kb on - strand, within CCNA_02009 at 2156.958 kb on + strand, within CCNA_02009 at 2156.985 kb on + strand, within CCNA_02009 at 2157.031 kb on + strand, within CCNA_02009 at 2157.047 kb on - strand, within CCNA_02009 at 2157.064 kb on + strand, within CCNA_02009 at 2157.071 kb on - strand at 2157.072 kb on - strand, within CCNA_02009 at 2157.072 kb on - strand, within CCNA_02009 at 2157.231 kb on + strand, within CCNA_02009 at 2157.231 kb on + strand, within CCNA_02009 at 2157.239 kb on - strand, within CCNA_02009 at 2157.277 kb on - strand at 2157.295 kb on - strand at 2157.327 kb on - strand at 2157.335 kb on + strand at 2157.335 kb on - strand at 2157.544 kb on - strand, within CCNA_02010 at 2157.557 kb on + strand at 2157.568 kb on + strand at 2157.576 kb on - strand at 2157.576 kb on - strand at 2157.576 kb on - strand at 2157.626 kb on + strand, within CCNA_02011 at 2157.648 kb on - strand, within CCNA_02011 at 2157.696 kb on - strand, within CCNA_02011 at 2157.696 kb on - strand, within CCNA_02011 at 2157.699 kb on + strand, within CCNA_02011 at 2157.729 kb on - strand, within CCNA_02011 at 2157.729 kb on - strand, within CCNA_02011 at 2157.735 kb on - strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.771 kb on - strand, within CCNA_02011 at 2157.771 kb on - strand, within CCNA_02011 at 2157.816 kb on - strand, within CCNA_02011 at 2157.866 kb on - strand, within CCNA_02011 at 2157.868 kb on - strand, within CCNA_02011 at 2157.910 kb on - strand, within CCNA_02011 at 2157.920 kb on - strand, within CCNA_02011 at 2157.978 kb on - strand at 2157.996 kb on - strand at 2157.996 kb on - strand at 2158.030 kb on + strand at 2158.036 kb on + strand at 2158.066 kb on - strand at 2158.087 kb on + strand at 2158.109 kb on + strand at 2158.137 kb on - strand at 2158.176 kb on - strand at 2158.176 kb on - strand at 2158.260 kb on + strand at 2158.268 kb on - strand, within CCNA_02012 at 2158.268 kb on - strand, within CCNA_02012 at 2158.268 kb on - strand, within CCNA_02012 at 2158.275 kb on - strand, within CCNA_02012 at 2158.295 kb on - strand, within CCNA_02012 at 2158.317 kb on - strand, within CCNA_02012 at 2158.445 kb on + strand, within CCNA_02012 at 2158.453 kb on - strand, within CCNA_02012 at 2158.567 kb on + strand, within CCNA_02012
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.03 mg/ml remove 2,156,914 - +1.4 2,156,925 - CCNA_02009 0.12 -0.7 2,156,958 + CCNA_02009 0.20 +0.4 2,156,985 + CCNA_02009 0.26 -0.4 2,157,031 + CCNA_02009 0.37 +0.7 2,157,047 - CCNA_02009 0.41 +1.2 2,157,064 + CCNA_02009 0.45 -0.7 2,157,071 - +2.3 2,157,072 - CCNA_02009 0.47 -0.7 2,157,072 - CCNA_02009 0.47 -0.2 2,157,231 + CCNA_02009 0.85 -3.1 2,157,231 + CCNA_02009 0.85 -1.1 2,157,239 - CCNA_02009 0.87 -1.2 2,157,277 - +1.2 2,157,295 - +0.9 2,157,327 - +2.3 2,157,335 + -0.6 2,157,335 - +0.8 2,157,544 - CCNA_02010 0.90 -1.6 2,157,557 + -0.0 2,157,568 + -0.1 2,157,576 - +1.4 2,157,576 - +1.2 2,157,576 - +1.3 2,157,626 + CCNA_02011 0.13 -0.2 2,157,648 - CCNA_02011 0.19 -0.1 2,157,696 - CCNA_02011 0.30 -0.5 2,157,696 - CCNA_02011 0.30 -1.7 2,157,699 + CCNA_02011 0.31 -1.1 2,157,729 - CCNA_02011 0.38 -1.1 2,157,729 - CCNA_02011 0.38 +1.0 2,157,735 - CCNA_02011 0.39 +0.2 2,157,763 + CCNA_02011 0.46 +1.1 2,157,763 + CCNA_02011 0.46 +0.8 2,157,771 - CCNA_02011 0.48 -0.3 2,157,771 - CCNA_02011 0.48 +0.9 2,157,816 - CCNA_02011 0.59 +0.0 2,157,866 - CCNA_02011 0.70 +0.7 2,157,868 - CCNA_02011 0.71 -0.9 2,157,910 - CCNA_02011 0.81 -0.2 2,157,920 - CCNA_02011 0.83 -0.3 2,157,978 - +0.5 2,157,996 - +0.9 2,157,996 - -0.1 2,158,030 + -0.6 2,158,036 + +0.1 2,158,066 - -1.0 2,158,087 + -0.0 2,158,109 + +0.8 2,158,137 - +0.4 2,158,176 - -1.6 2,158,176 - +0.7 2,158,260 + +0.6 2,158,268 - CCNA_02012 0.10 +0.2 2,158,268 - CCNA_02012 0.10 +0.8 2,158,268 - CCNA_02012 0.10 -1.8 2,158,275 - CCNA_02012 0.11 +1.0 2,158,295 - CCNA_02012 0.12 +0.9 2,158,317 - CCNA_02012 0.13 +0.8 2,158,445 + CCNA_02012 0.21 +0.6 2,158,453 - CCNA_02012 0.21 -2.2 2,158,567 + CCNA_02012 0.28 -0.5
Or see this region's nucleotide sequence