Experiment: PYE with Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01966 and CCNA_01967 are separated by 71 nucleotides CCNA_01967 and CCNA_01968 are separated by 94 nucleotides CCNA_01968 and CCNA_01969 are separated by 77 nucleotides
CCNA_01966: CCNA_01966 - vitamin B12-dependent ribonucleotide reductase, at 2,108,222 to 2,110,945
_01966
CCNA_01967: CCNA_01967 - hypothetical protein with pentapeptide repeats, at 2,111,017 to 2,111,514
_01967
CCNA_01968: CCNA_01968 - pentapeptide repeats containing protein, at 2,111,609 to 2,112,478
_01968
CCNA_01969: CCNA_01969 - aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (from data) , at 2,112,556 to 2,114,394
_01969
Position (kb)
2111
2112
2113 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2110.621 kb on + strand, within CCNA_01966 at 2110.629 kb on - strand, within CCNA_01966 at 2110.629 kb on - strand, within CCNA_01966 at 2110.642 kb on + strand, within CCNA_01966 at 2110.642 kb on + strand, within CCNA_01966 at 2110.642 kb on + strand, within CCNA_01966 at 2110.642 kb on + strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.650 kb on - strand, within CCNA_01966 at 2110.655 kb on + strand, within CCNA_01966 at 2110.662 kb on + strand, within CCNA_01966 at 2110.675 kb on - strand at 2110.793 kb on - strand at 2110.881 kb on - strand at 2110.921 kb on + strand at 2110.921 kb on + strand at 2110.959 kb on + strand at 2110.959 kb on + strand at 2110.959 kb on + strand at 2110.967 kb on - strand at 2110.967 kb on - strand at 2110.979 kb on + strand at 2110.981 kb on - strand at 2111.023 kb on - strand at 2111.045 kb on + strand at 2111.153 kb on - strand, within CCNA_01967 at 2111.153 kb on - strand, within CCNA_01967 at 2111.170 kb on + strand, within CCNA_01967 at 2111.230 kb on + strand, within CCNA_01967 at 2111.290 kb on + strand, within CCNA_01967 at 2111.325 kb on + strand, within CCNA_01967 at 2111.328 kb on - strand, within CCNA_01967 at 2111.375 kb on + strand, within CCNA_01967 at 2111.451 kb on - strand, within CCNA_01967 at 2111.550 kb on - strand at 2111.563 kb on - strand at 2111.585 kb on + strand at 2111.593 kb on - strand at 2111.600 kb on + strand at 2111.608 kb on - strand at 2111.708 kb on + strand, within CCNA_01968 at 2111.923 kb on + strand, within CCNA_01968 at 2112.332 kb on + strand, within CCNA_01968 at 2112.378 kb on + strand, within CCNA_01968 at 2112.378 kb on + strand, within CCNA_01968 at 2112.378 kb on + strand, within CCNA_01968 at 2112.422 kb on + strand at 2112.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.03 mg/ml remove 2,110,621 + CCNA_01966 0.88 +2.2 2,110,629 - CCNA_01966 0.88 -1.9 2,110,629 - CCNA_01966 0.88 -0.2 2,110,642 + CCNA_01966 0.89 +1.0 2,110,642 + CCNA_01966 0.89 +1.9 2,110,642 + CCNA_01966 0.89 -0.0 2,110,642 + CCNA_01966 0.89 +0.5 2,110,650 - CCNA_01966 0.89 -0.7 2,110,650 - CCNA_01966 0.89 +2.0 2,110,650 - CCNA_01966 0.89 -1.9 2,110,650 - CCNA_01966 0.89 +0.4 2,110,650 - CCNA_01966 0.89 -1.6 2,110,655 + CCNA_01966 0.89 -0.5 2,110,662 + CCNA_01966 0.90 +1.3 2,110,675 - -1.5 2,110,793 - -1.2 2,110,881 - -1.8 2,110,921 + -0.6 2,110,921 + +2.1 2,110,959 + +0.1 2,110,959 + +0.1 2,110,959 + +0.3 2,110,967 - +1.8 2,110,967 - +0.8 2,110,979 + +0.4 2,110,981 - -1.3 2,111,023 - -0.6 2,111,045 + -1.2 2,111,153 - CCNA_01967 0.27 -2.9 2,111,153 - CCNA_01967 0.27 +0.5 2,111,170 + CCNA_01967 0.31 -0.2 2,111,230 + CCNA_01967 0.43 +0.6 2,111,290 + CCNA_01967 0.55 -1.6 2,111,325 + CCNA_01967 0.62 +0.6 2,111,328 - CCNA_01967 0.62 -0.9 2,111,375 + CCNA_01967 0.72 +0.5 2,111,451 - CCNA_01967 0.87 -1.5 2,111,550 - +1.3 2,111,563 - +1.9 2,111,585 + +1.2 2,111,593 - +0.1 2,111,600 + +1.3 2,111,608 - -0.5 2,111,708 + CCNA_01968 0.11 +1.1 2,111,923 + CCNA_01968 0.36 -0.8 2,112,332 + CCNA_01968 0.83 +0.9 2,112,378 + CCNA_01968 0.88 +3.2 2,112,378 + CCNA_01968 0.88 +2.8 2,112,378 + CCNA_01968 0.88 +3.0 2,112,422 + +3.2 2,112,557 - +2.0
Or see this region's nucleotide sequence