Experiment: PYE with Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01580 and CCNA_01581 are separated by 108 nucleotides CCNA_01581 and CCNA_01582 are separated by 114 nucleotides CCNA_01582 and CCNA_01583 are separated by 69 nucleotides CCNA_01583 and CCNA_03954 overlap by 239 nucleotides CCNA_03954 and CCNA_01584 are separated by 75 nucleotides
CCNA_01580: CCNA_01580 - M15 superfamily membrane peptidase, at 1,696,883 to 1,697,533
_01580
CCNA_01581: CCNA_01581 - hypothetical protein, at 1,697,642 to 1,697,974
_01581
CCNA_01582: CCNA_01582 - Glutathione-dependent formaldehyde-activating enzyme, at 1,698,089 to 1,698,451
_01582
CCNA_01583: CCNA_01583 - phosphate-binding protein, at 1,698,521 to 1,699,561
_01583
CCNA_03954: CCNA_03954 - hypothetical protein, at 1,699,323 to 1,699,610
_03954
CCNA_01584: CCNA_01584 - multimodular transpeptidase-transglycosylase PBP 1A, at 1,699,686 to 1,702,070
_01584
Position (kb)
1698
1699
1700 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1697.545 kb on + strand at 1697.584 kb on + strand at 1697.612 kb on - strand at 1697.634 kb on + strand at 1697.634 kb on + strand at 1697.644 kb on - strand at 1697.644 kb on - strand at 1697.651 kb on + strand at 1697.669 kb on + strand at 1697.760 kb on + strand, within CCNA_01581 at 1697.773 kb on - strand, within CCNA_01581 at 1697.829 kb on - strand, within CCNA_01581 at 1697.850 kb on - strand, within CCNA_01581 at 1697.850 kb on - strand, within CCNA_01581 at 1698.002 kb on - strand at 1698.018 kb on + strand at 1698.018 kb on + strand at 1698.126 kb on + strand, within CCNA_01582 at 1698.136 kb on + strand, within CCNA_01582 at 1698.237 kb on - strand, within CCNA_01582 at 1698.389 kb on - strand, within CCNA_01582 at 1698.396 kb on - strand, within CCNA_01582 at 1698.406 kb on + strand, within CCNA_01582 at 1698.421 kb on + strand at 1698.429 kb on - strand at 1698.721 kb on - strand, within CCNA_01583 at 1698.721 kb on - strand, within CCNA_01583 at 1698.762 kb on + strand, within CCNA_01583 at 1698.895 kb on - strand, within CCNA_01583 at 1699.027 kb on + strand, within CCNA_01583 at 1699.036 kb on + strand at 1699.044 kb on + strand, within CCNA_01583 at 1699.152 kb on - strand, within CCNA_01583 at 1699.164 kb on + strand, within CCNA_01583 at 1699.342 kb on - strand at 1699.484 kb on - strand at 1699.484 kb on - strand at 1699.578 kb on - strand, within CCNA_03954 at 1699.597 kb on - strand at 1699.607 kb on - strand at 1699.607 kb on - strand at 1699.678 kb on - strand at 1699.726 kb on - strand at 1699.786 kb on - strand at 1699.899 kb on - strand at 1699.939 kb on - strand, within CCNA_01584 at 1699.941 kb on + strand, within CCNA_01584 at 1699.971 kb on + strand, within CCNA_01584 at 1700.000 kb on - strand, within CCNA_01584 at 1700.013 kb on + strand, within CCNA_01584 at 1700.016 kb on - strand, within CCNA_01584 at 1700.021 kb on - strand, within CCNA_01584 at 1700.031 kb on - strand, within CCNA_01584 at 1700.063 kb on + strand, within CCNA_01584 at 1700.086 kb on + strand, within CCNA_01584 at 1700.124 kb on + strand, within CCNA_01584 at 1700.137 kb on + strand, within CCNA_01584 at 1700.139 kb on - strand, within CCNA_01584 at 1700.170 kb on - strand, within CCNA_01584 at 1700.202 kb on + strand, within CCNA_01584 at 1700.232 kb on + strand, within CCNA_01584 at 1700.240 kb on - strand, within CCNA_01584 at 1700.240 kb on - strand, within CCNA_01584 at 1700.244 kb on + strand, within CCNA_01584 at 1700.245 kb on - strand, within CCNA_01584 at 1700.250 kb on - strand, within CCNA_01584 at 1700.269 kb on - strand, within CCNA_01584 at 1700.276 kb on + strand, within CCNA_01584 at 1700.291 kb on + strand at 1700.299 kb on - strand, within CCNA_01584 at 1700.302 kb on + strand, within CCNA_01584 at 1700.320 kb on - strand, within CCNA_01584 at 1700.375 kb on - strand, within CCNA_01584 at 1700.390 kb on - strand, within CCNA_01584 at 1700.403 kb on + strand, within CCNA_01584 at 1700.411 kb on - strand, within CCNA_01584 at 1700.434 kb on + strand, within CCNA_01584 at 1700.439 kb on + strand, within CCNA_01584 at 1700.441 kb on + strand, within CCNA_01584 at 1700.452 kb on - strand, within CCNA_01584 at 1700.475 kb on + strand, within CCNA_01584 at 1700.483 kb on - strand, within CCNA_01584 at 1700.490 kb on - strand, within CCNA_01584 at 1700.553 kb on + strand, within CCNA_01584 at 1700.561 kb on + strand, within CCNA_01584
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.03 mg/ml remove 1,697,545 + -0.1 1,697,584 + +0.8 1,697,612 - +1.4 1,697,634 + -0.5 1,697,634 + +1.2 1,697,644 - -2.3 1,697,644 - -1.1 1,697,651 + -0.0 1,697,669 + -0.3 1,697,760 + CCNA_01581 0.35 +2.7 1,697,773 - CCNA_01581 0.39 +1.3 1,697,829 - CCNA_01581 0.56 -1.5 1,697,850 - CCNA_01581 0.62 -0.5 1,697,850 - CCNA_01581 0.62 +0.4 1,698,002 - -0.5 1,698,018 + -1.7 1,698,018 + +1.6 1,698,126 + CCNA_01582 0.10 +0.6 1,698,136 + CCNA_01582 0.13 -1.1 1,698,237 - CCNA_01582 0.41 +1.2 1,698,389 - CCNA_01582 0.83 -1.1 1,698,396 - CCNA_01582 0.85 -1.0 1,698,406 + CCNA_01582 0.87 +0.8 1,698,421 + -1.0 1,698,429 - -0.9 1,698,721 - CCNA_01583 0.19 +1.5 1,698,721 - CCNA_01583 0.19 +3.9 1,698,762 + CCNA_01583 0.23 +1.7 1,698,895 - CCNA_01583 0.36 +4.8 1,699,027 + CCNA_01583 0.49 +3.6 1,699,036 + +2.0 1,699,044 + CCNA_01583 0.50 +0.6 1,699,152 - CCNA_01583 0.61 +3.3 1,699,164 + CCNA_01583 0.62 +2.6 1,699,342 - +4.1 1,699,484 - +3.2 1,699,484 - +3.6 1,699,578 - CCNA_03954 0.89 +1.7 1,699,597 - -0.3 1,699,607 - +1.1 1,699,607 - +0.9 1,699,678 - +1.6 1,699,726 - -0.3 1,699,786 - -1.8 1,699,899 - +0.6 1,699,939 - CCNA_01584 0.11 -0.1 1,699,941 + CCNA_01584 0.11 +0.2 1,699,971 + CCNA_01584 0.12 +1.4 1,700,000 - CCNA_01584 0.13 +2.6 1,700,013 + CCNA_01584 0.14 +0.4 1,700,016 - CCNA_01584 0.14 +1.0 1,700,021 - CCNA_01584 0.14 +0.3 1,700,031 - CCNA_01584 0.14 +0.8 1,700,063 + CCNA_01584 0.16 +0.2 1,700,086 + CCNA_01584 0.17 -0.7 1,700,124 + CCNA_01584 0.18 +0.0 1,700,137 + CCNA_01584 0.19 -1.1 1,700,139 - CCNA_01584 0.19 -1.5 1,700,170 - CCNA_01584 0.20 -1.1 1,700,202 + CCNA_01584 0.22 +1.7 1,700,232 + CCNA_01584 0.23 -1.6 1,700,240 - CCNA_01584 0.23 +1.9 1,700,240 - CCNA_01584 0.23 -0.0 1,700,244 + CCNA_01584 0.23 -0.4 1,700,245 - CCNA_01584 0.23 -0.3 1,700,250 - CCNA_01584 0.24 +0.8 1,700,269 - CCNA_01584 0.24 +1.1 1,700,276 + CCNA_01584 0.25 +2.2 1,700,291 + +1.2 1,700,299 - CCNA_01584 0.26 +0.6 1,700,302 + CCNA_01584 0.26 -2.2 1,700,320 - CCNA_01584 0.27 +0.0 1,700,375 - CCNA_01584 0.29 +1.2 1,700,390 - CCNA_01584 0.30 +0.5 1,700,403 + CCNA_01584 0.30 +2.4 1,700,411 - CCNA_01584 0.30 +0.3 1,700,434 + CCNA_01584 0.31 +2.2 1,700,439 + CCNA_01584 0.32 +0.3 1,700,441 + CCNA_01584 0.32 -1.3 1,700,452 - CCNA_01584 0.32 +0.9 1,700,475 + CCNA_01584 0.33 -1.1 1,700,483 - CCNA_01584 0.33 +0.1 1,700,490 - CCNA_01584 0.34 +1.5 1,700,553 + CCNA_01584 0.36 +0.2 1,700,561 + CCNA_01584 0.37 +0.4
Or see this region's nucleotide sequence