Experiment: PYE with Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00742 and CCNA_00743 are separated by 146 nucleotides CCNA_00743 and CCNA_00744 are separated by 241 nucleotides CCNA_00744 and CCNA_00745 are separated by 33 nucleotides CCNA_00745 and CCNA_03935 overlap by 693 nucleotides CCNA_03935 and CCNA_00746 are separated by 251 nucleotides
CCNA_00742: CCNA_00742 - HAD superfamily sugar phosphatase, at 799,937 to 800,797
_00742
CCNA_00743: CCNA_00743 - NnrU family membrane protein, at 800,944 to 801,510
_00743
CCNA_00744: CCNA_00744 - GIY-YIG endonuclease domain protein, at 801,752 to 802,036
_00744
CCNA_00745: CCNA_00745 - hypothetical protein, at 802,070 to 802,774
_00745
CCNA_03935: CCNA_03935 - hypothetical protein, at 802,082 to 802,519
_03935
CCNA_00746: CCNA_00746 - ADA regulatory protein/O6-methylguanine-DNA, at 802,771 to 803,496
_00746
Position (kb) 801
802
803 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 800.855 kb on + strand at 800.855 kb on + strand at 800.858 kb on - strand at 800.863 kb on - strand at 800.863 kb on - strand at 800.963 kb on + strand at 800.973 kb on + strand at 800.973 kb on + strand at 800.981 kb on - strand at 800.981 kb on - strand at 800.981 kb on - strand at 800.990 kb on - strand at 800.992 kb on + strand at 801.039 kb on + strand, within CCNA_00743 at 801.039 kb on + strand, within CCNA_00743 at 801.091 kb on - strand, within CCNA_00743 at 801.130 kb on + strand, within CCNA_00743 at 801.135 kb on + strand, within CCNA_00743 at 801.135 kb on + strand, within CCNA_00743 at 801.177 kb on - strand, within CCNA_00743 at 801.185 kb on + strand, within CCNA_00743 at 801.199 kb on + strand, within CCNA_00743 at 801.199 kb on + strand, within CCNA_00743 at 801.199 kb on + strand, within CCNA_00743 at 801.207 kb on - strand, within CCNA_00743 at 801.207 kb on - strand, within CCNA_00743 at 801.222 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.244 kb on - strand, within CCNA_00743 at 801.266 kb on + strand, within CCNA_00743 at 801.274 kb on - strand, within CCNA_00743 at 801.291 kb on + strand, within CCNA_00743 at 801.305 kb on - strand, within CCNA_00743 at 801.320 kb on - strand, within CCNA_00743 at 801.322 kb on - strand, within CCNA_00743 at 801.337 kb on + strand, within CCNA_00743 at 801.342 kb on + strand, within CCNA_00743 at 801.342 kb on + strand, within CCNA_00743 at 801.345 kb on - strand, within CCNA_00743 at 801.345 kb on - strand, within CCNA_00743 at 801.392 kb on + strand, within CCNA_00743 at 801.401 kb on + strand, within CCNA_00743 at 801.402 kb on + strand, within CCNA_00743 at 801.402 kb on + strand, within CCNA_00743 at 801.410 kb on - strand, within CCNA_00743 at 801.418 kb on + strand, within CCNA_00743 at 801.418 kb on + strand, within CCNA_00743 at 801.418 kb on + strand, within CCNA_00743 at 801.435 kb on + strand, within CCNA_00743 at 801.438 kb on - strand, within CCNA_00743 at 801.443 kb on - strand, within CCNA_00743 at 801.469 kb on + strand at 801.483 kb on + strand at 801.506 kb on + strand at 801.556 kb on + strand at 801.564 kb on + strand at 801.564 kb on + strand at 801.572 kb on - strand at 801.572 kb on - strand at 801.596 kb on + strand at 801.596 kb on + strand at 801.596 kb on + strand at 801.604 kb on - strand at 801.604 kb on - strand at 801.604 kb on - strand at 801.623 kb on - strand at 801.628 kb on - strand at 801.628 kb on - strand at 801.628 kb on - strand at 801.643 kb on + strand at 801.666 kb on + strand at 801.669 kb on - strand at 801.674 kb on - strand at 801.764 kb on + strand at 801.764 kb on + strand at 801.777 kb on - strand at 801.797 kb on + strand, within CCNA_00744 at 801.801 kb on + strand, within CCNA_00744 at 801.801 kb on + strand, within CCNA_00744 at 801.809 kb on - strand, within CCNA_00744 at 801.812 kb on + strand, within CCNA_00744 at 801.842 kb on + strand, within CCNA_00744 at 801.842 kb on - strand, within CCNA_00744 at 801.862 kb on - strand, within CCNA_00744 at 801.867 kb on - strand, within CCNA_00744 at 801.892 kb on + strand, within CCNA_00744 at 801.892 kb on + strand, within CCNA_00744 at 801.892 kb on + strand, within CCNA_00744 at 801.939 kb on - strand, within CCNA_00744 at 801.945 kb on + strand, within CCNA_00744 at 801.965 kb on - strand, within CCNA_00744 at 801.989 kb on - strand, within CCNA_00744 at 801.989 kb on - strand, within CCNA_00744 at 802.047 kb on - strand at 802.054 kb on - strand at 802.061 kb on + strand at 802.061 kb on + strand at 802.061 kb on + strand at 802.061 kb on + strand at 802.064 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.070 kb on + strand at 802.080 kb on - strand at 802.093 kb on + strand at 802.101 kb on - strand at 802.132 kb on + strand at 802.140 kb on - strand at 802.184 kb on + strand at 802.192 kb on - strand at 802.192 kb on - strand at 802.199 kb on + strand at 802.207 kb on - strand at 802.214 kb on - strand at 802.269 kb on - strand at 802.302 kb on + strand at 802.310 kb on - strand at 802.330 kb on + strand at 802.370 kb on + strand at 802.393 kb on - strand at 802.399 kb on - strand at 802.400 kb on + strand at 802.415 kb on + strand at 802.423 kb on - strand at 802.423 kb on - strand at 802.423 kb on - strand at 802.438 kb on + strand at 802.446 kb on + strand at 802.494 kb on + strand at 802.502 kb on - strand at 802.502 kb on - strand at 802.519 kb on - strand at 802.534 kb on - strand, within CCNA_00745 at 802.571 kb on + strand, within CCNA_00745 at 802.578 kb on + strand, within CCNA_00745 at 802.580 kb on - strand, within CCNA_00745 at 802.586 kb on - strand, within CCNA_00745 at 802.594 kb on + strand, within CCNA_00745 at 802.594 kb on + strand, within CCNA_00745 at 802.602 kb on - strand, within CCNA_00745 at 802.618 kb on - strand, within CCNA_00745 at 802.658 kb on + strand, within CCNA_00745 at 802.680 kb on + strand, within CCNA_00745 at 802.685 kb on + strand, within CCNA_00745 at 802.693 kb on - strand, within CCNA_00745 at 802.705 kb on - strand at 802.705 kb on - strand at 802.705 kb on - strand at 802.739 kb on + strand at 802.739 kb on + strand at 802.745 kb on + strand at 802.745 kb on + strand at 802.856 kb on - strand, within CCNA_00746 at 802.865 kb on + strand, within CCNA_00746 at 802.873 kb on - strand, within CCNA_00746 at 802.884 kb on + strand, within CCNA_00746 at 802.897 kb on + strand, within CCNA_00746 at 802.906 kb on - strand, within CCNA_00746 at 802.919 kb on + strand, within CCNA_00746 at 802.919 kb on + strand at 802.919 kb on + strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.932 kb on - strand, within CCNA_00746 at 802.950 kb on + strand, within CCNA_00746 at 802.952 kb on + strand at 802.992 kb on - strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.011 kb on - strand, within CCNA_00746 at 803.011 kb on - strand, within CCNA_00746 at 803.011 kb on - strand, within CCNA_00746 at 803.020 kb on + strand, within CCNA_00746 at 803.028 kb on - strand, within CCNA_00746 at 803.028 kb on - strand, within CCNA_00746
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.03 mg/ml remove 800,855 + -1.0 800,855 + +1.1 800,858 - -2.1 800,863 - -0.5 800,863 - -3.0 800,963 + -0.6 800,973 + -1.2 800,973 + +1.1 800,981 - +0.3 800,981 - +0.6 800,981 - +0.4 800,990 - -0.9 800,992 + +0.1 801,039 + CCNA_00743 0.17 -0.7 801,039 + CCNA_00743 0.17 +0.7 801,091 - CCNA_00743 0.26 -0.5 801,130 + CCNA_00743 0.33 -0.5 801,135 + CCNA_00743 0.34 -0.8 801,135 + CCNA_00743 0.34 +0.8 801,177 - CCNA_00743 0.41 -1.0 801,185 + CCNA_00743 0.43 +1.6 801,199 + CCNA_00743 0.45 -0.8 801,199 + CCNA_00743 0.45 -0.6 801,199 + CCNA_00743 0.45 +0.6 801,207 - CCNA_00743 0.46 -0.8 801,207 - CCNA_00743 0.46 +0.9 801,222 + CCNA_00743 0.49 +0.8 801,231 + CCNA_00743 0.51 +0.8 801,231 + CCNA_00743 0.51 +1.0 801,231 + CCNA_00743 0.51 +0.4 801,231 + CCNA_00743 0.51 +0.6 801,231 + CCNA_00743 0.51 +1.8 801,239 - CCNA_00743 0.52 +2.1 801,239 - CCNA_00743 0.52 -1.0 801,239 - CCNA_00743 0.52 -1.0 801,239 - CCNA_00743 0.52 +0.2 801,239 - CCNA_00743 0.52 +0.8 801,244 - CCNA_00743 0.53 -0.9 801,266 + CCNA_00743 0.57 -0.9 801,274 - CCNA_00743 0.58 +0.1 801,291 + CCNA_00743 0.61 -0.1 801,305 - CCNA_00743 0.64 -1.9 801,320 - CCNA_00743 0.66 -0.7 801,322 - CCNA_00743 0.67 +1.3 801,337 + CCNA_00743 0.69 +0.5 801,342 + CCNA_00743 0.70 -0.1 801,342 + CCNA_00743 0.70 -0.3 801,345 - CCNA_00743 0.71 +1.5 801,345 - CCNA_00743 0.71 -0.6 801,392 + CCNA_00743 0.79 -0.1 801,401 + CCNA_00743 0.81 +1.0 801,402 + CCNA_00743 0.81 +1.3 801,402 + CCNA_00743 0.81 -0.5 801,410 - CCNA_00743 0.82 -0.2 801,418 + CCNA_00743 0.84 +1.0 801,418 + CCNA_00743 0.84 -1.1 801,418 + CCNA_00743 0.84 -0.3 801,435 + CCNA_00743 0.87 -0.9 801,438 - CCNA_00743 0.87 +0.6 801,443 - CCNA_00743 0.88 +1.1 801,469 + +1.2 801,483 + -1.2 801,506 + -0.3 801,556 + +1.0 801,564 + +0.1 801,564 + -0.2 801,572 - -1.7 801,572 - -1.9 801,596 + -0.2 801,596 + -0.7 801,596 + -1.4 801,604 - +1.7 801,604 - +0.5 801,604 - +0.3 801,623 - -1.3 801,628 - -1.1 801,628 - +0.6 801,628 - -0.2 801,643 + -1.1 801,666 + +1.1 801,669 - -1.4 801,674 - -1.2 801,764 + +0.4 801,764 + +0.6 801,777 - -0.1 801,797 + CCNA_00744 0.16 -0.8 801,801 + CCNA_00744 0.17 -1.4 801,801 + CCNA_00744 0.17 -0.8 801,809 - CCNA_00744 0.20 -0.2 801,812 + CCNA_00744 0.21 -0.5 801,842 + CCNA_00744 0.32 +1.3 801,842 - CCNA_00744 0.32 +0.6 801,862 - CCNA_00744 0.39 -0.0 801,867 - CCNA_00744 0.40 -1.3 801,892 + CCNA_00744 0.49 -0.9 801,892 + CCNA_00744 0.49 -2.9 801,892 + CCNA_00744 0.49 -1.3 801,939 - CCNA_00744 0.66 -0.3 801,945 + CCNA_00744 0.68 -2.2 801,965 - CCNA_00744 0.75 +0.8 801,989 - CCNA_00744 0.83 +0.0 801,989 - CCNA_00744 0.83 +1.0 802,047 - +1.2 802,054 - -0.5 802,061 + -0.3 802,061 + -2.1 802,061 + -0.5 802,061 + +0.6 802,064 - -0.8 802,069 - -0.2 802,069 - +0.7 802,069 - +0.9 802,069 - +1.2 802,069 - -2.0 802,069 - +0.6 802,070 + -0.8 802,080 - +0.1 802,093 + +0.1 802,101 - -0.6 802,132 + -1.0 802,140 - +1.6 802,184 + -1.9 802,192 - -0.7 802,192 - -1.5 802,199 + -0.1 802,207 - +0.0 802,214 - +1.5 802,269 - +1.6 802,302 + +1.6 802,310 - -0.7 802,330 + +1.3 802,370 + +1.5 802,393 - -1.5 802,399 - -0.2 802,400 + +1.3 802,415 + +1.3 802,423 - +0.1 802,423 - -1.2 802,423 - +0.5 802,438 + -1.0 802,446 + +0.5 802,494 + +0.8 802,502 - +0.9 802,502 - -1.2 802,519 - +0.6 802,534 - CCNA_00745 0.66 -1.0 802,571 + CCNA_00745 0.71 -1.4 802,578 + CCNA_00745 0.72 -0.9 802,580 - CCNA_00745 0.72 +1.6 802,586 - CCNA_00745 0.73 +0.1 802,594 + CCNA_00745 0.74 -2.2 802,594 + CCNA_00745 0.74 +0.2 802,602 - CCNA_00745 0.75 -1.4 802,618 - CCNA_00745 0.78 -1.5 802,658 + CCNA_00745 0.83 -0.6 802,680 + CCNA_00745 0.87 -2.5 802,685 + CCNA_00745 0.87 -2.2 802,693 - CCNA_00745 0.88 +0.1 802,705 - -1.3 802,705 - -1.1 802,705 - +0.6 802,739 + -2.0 802,739 + -2.2 802,745 + +0.0 802,745 + -1.9 802,856 - CCNA_00746 0.12 +0.5 802,865 + CCNA_00746 0.13 -0.7 802,873 - CCNA_00746 0.14 -1.5 802,884 + CCNA_00746 0.16 -2.0 802,897 + CCNA_00746 0.17 -1.5 802,906 - CCNA_00746 0.19 -1.7 802,919 + CCNA_00746 0.20 +0.6 802,919 + +2.3 802,919 + CCNA_00746 0.20 +0.2 802,927 - CCNA_00746 0.21 +1.1 802,927 - CCNA_00746 0.21 +0.4 802,927 - CCNA_00746 0.21 +0.9 802,927 - CCNA_00746 0.21 -0.2 802,932 - CCNA_00746 0.22 -0.1 802,950 + CCNA_00746 0.25 +0.3 802,952 + +2.3 802,992 - CCNA_00746 0.30 +1.6 803,003 + CCNA_00746 0.32 +1.3 803,003 + CCNA_00746 0.32 +4.7 803,003 + CCNA_00746 0.32 -1.9 803,003 + CCNA_00746 0.32 +0.2 803,011 - CCNA_00746 0.33 -1.2 803,011 - CCNA_00746 0.33 +1.1 803,011 - CCNA_00746 0.33 +1.0 803,020 + CCNA_00746 0.34 +1.9 803,028 - CCNA_00746 0.35 -0.4 803,028 - CCNA_00746 0.35 -0.0
Or see this region's nucleotide sequence