Experiment: PYE with Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00559 and CCNA_00560 are separated by 77 nucleotides CCNA_00560 and CCNA_00561 overlap by 4 nucleotides CCNA_00561 and CCNA_00562 overlap by 4 nucleotides CCNA_00562 and CCNA_00563 overlap by 4 nucleotides CCNA_00563 and CCNA_00564 are separated by 9 nucleotides
CCNA_00559: CCNA_00559 - homoserine O-acetyltransferase, at 574,589 to 575,737
_00559
CCNA_00560: CCNA_00560 - phosphohistidine phosphatase SixA, at 575,815 to 576,327
_00560
CCNA_00561: CCNA_00561 - conserved hypothetical cytosolic protein, at 576,324 to 576,668
_00561
CCNA_00562: CCNA_00562 - dihydroorotate dehydrogenase, at 576,665 to 577,723
_00562
CCNA_00563: CCNA_00563 - SNARE-associated family membrane protein, at 577,720 to 578,457
_00563
CCNA_00564: CCNA_00564 - sensory transduction protein kinase CenK, at 578,467 to 579,972
_00564
Position (kb) 576
577
578 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 575.674 kb on - strand at 575.739 kb on - strand at 575.741 kb on - strand at 575.749 kb on - strand at 575.797 kb on - strand at 575.799 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.801 kb on + strand at 575.807 kb on - strand at 575.807 kb on - strand at 575.809 kb on - strand at 575.818 kb on + strand at 575.835 kb on - strand at 575.841 kb on + strand at 575.847 kb on + strand at 575.847 kb on + strand at 575.860 kb on + strand at 575.860 kb on + strand at 575.868 kb on - strand, within CCNA_00560 at 575.868 kb on - strand, within CCNA_00560 at 575.898 kb on + strand, within CCNA_00560 at 575.900 kb on - strand, within CCNA_00560 at 576.001 kb on + strand, within CCNA_00560 at 576.027 kb on - strand, within CCNA_00560 at 576.031 kb on - strand, within CCNA_00560 at 576.048 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.057 kb on + strand, within CCNA_00560 at 576.065 kb on - strand, within CCNA_00560 at 576.175 kb on + strand, within CCNA_00560 at 576.175 kb on + strand, within CCNA_00560 at 576.188 kb on - strand, within CCNA_00560 at 576.191 kb on + strand, within CCNA_00560 at 576.244 kb on + strand, within CCNA_00560 at 576.263 kb on - strand, within CCNA_00560 at 576.270 kb on - strand, within CCNA_00560 at 576.288 kb on + strand at 576.288 kb on + strand at 576.340 kb on - strand at 576.342 kb on + strand at 576.342 kb on + strand at 576.342 kb on + strand at 576.342 kb on + strand at 576.350 kb on - strand at 576.350 kb on - strand at 576.357 kb on + strand at 576.357 kb on + strand at 576.375 kb on + strand, within CCNA_00561 at 576.375 kb on + strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.383 kb on - strand, within CCNA_00561 at 576.431 kb on - strand, within CCNA_00561 at 576.455 kb on - strand, within CCNA_00561 at 576.455 kb on - strand, within CCNA_00561 at 576.470 kb on + strand, within CCNA_00561 at 576.478 kb on - strand, within CCNA_00561 at 576.528 kb on + strand, within CCNA_00561 at 576.536 kb on - strand, within CCNA_00561 at 576.536 kb on - strand at 576.536 kb on - strand, within CCNA_00561 at 576.580 kb on - strand, within CCNA_00561 at 576.600 kb on - strand, within CCNA_00561 at 576.615 kb on + strand, within CCNA_00561 at 576.669 kb on + strand at 576.695 kb on + strand at 576.695 kb on + strand at 576.703 kb on - strand at 576.703 kb on - strand at 576.703 kb on - strand at 576.727 kb on - strand at 576.742 kb on + strand at 576.747 kb on + strand at 576.750 kb on - strand at 576.761 kb on - strand at 576.761 kb on - strand at 576.761 kb on - strand at 576.783 kb on + strand, within CCNA_00562 at 576.810 kb on + strand, within CCNA_00562 at 576.818 kb on - strand, within CCNA_00562 at 576.841 kb on - strand, within CCNA_00562 at 576.890 kb on + strand, within CCNA_00562 at 576.905 kb on - strand, within CCNA_00562 at 576.915 kb on + strand, within CCNA_00562 at 576.983 kb on + strand, within CCNA_00562 at 576.998 kb on + strand, within CCNA_00562 at 577.006 kb on - strand, within CCNA_00562 at 577.006 kb on - strand, within CCNA_00562 at 577.054 kb on - strand, within CCNA_00562 at 577.072 kb on - strand, within CCNA_00562 at 577.087 kb on - strand, within CCNA_00562 at 577.097 kb on - strand, within CCNA_00562 at 577.148 kb on + strand, within CCNA_00562 at 577.188 kb on + strand, within CCNA_00562 at 577.188 kb on + strand, within CCNA_00562 at 577.196 kb on - strand, within CCNA_00562 at 577.196 kb on - strand, within CCNA_00562 at 577.205 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.210 kb on + strand, within CCNA_00562 at 577.218 kb on - strand, within CCNA_00562 at 577.218 kb on - strand, within CCNA_00562 at 577.262 kb on + strand, within CCNA_00562 at 577.270 kb on - strand, within CCNA_00562 at 577.272 kb on - strand, within CCNA_00562 at 577.311 kb on + strand, within CCNA_00562 at 577.319 kb on - strand, within CCNA_00562 at 577.333 kb on + strand, within CCNA_00562 at 577.333 kb on + strand, within CCNA_00562 at 577.342 kb on - strand, within CCNA_00562 at 577.342 kb on - strand, within CCNA_00562 at 577.347 kb on - strand, within CCNA_00562 at 577.357 kb on + strand, within CCNA_00562 at 577.369 kb on + strand, within CCNA_00562 at 577.370 kb on - strand, within CCNA_00562 at 577.385 kb on - strand, within CCNA_00562 at 577.405 kb on - strand, within CCNA_00562 at 577.439 kb on - strand, within CCNA_00562 at 577.474 kb on + strand, within CCNA_00562 at 577.526 kb on + strand, within CCNA_00562 at 577.571 kb on + strand, within CCNA_00562 at 577.598 kb on + strand, within CCNA_00562 at 577.603 kb on + strand, within CCNA_00562 at 577.603 kb on + strand, within CCNA_00562 at 577.606 kb on - strand, within CCNA_00562 at 577.608 kb on + strand, within CCNA_00562 at 577.613 kb on + strand, within CCNA_00562 at 577.616 kb on - strand, within CCNA_00562 at 577.618 kb on + strand at 577.626 kb on - strand at 577.629 kb on + strand at 577.634 kb on + strand at 577.702 kb on - strand at 577.726 kb on - strand at 577.732 kb on - strand at 577.735 kb on + strand at 577.735 kb on + strand at 577.735 kb on + strand at 577.743 kb on - strand at 577.754 kb on + strand at 577.773 kb on + strand at 577.793 kb on - strand at 577.887 kb on + strand, within CCNA_00563 at 577.887 kb on + strand, within CCNA_00563 at 577.887 kb on + strand, within CCNA_00563 at 577.895 kb on - strand, within CCNA_00563 at 577.899 kb on + strand, within CCNA_00563 at 577.957 kb on - strand, within CCNA_00563 at 578.000 kb on + strand, within CCNA_00563 at 578.035 kb on + strand, within CCNA_00563 at 578.043 kb on - strand, within CCNA_00563 at 578.082 kb on - strand, within CCNA_00563 at 578.082 kb on - strand, within CCNA_00563 at 578.082 kb on - strand, within CCNA_00563 at 578.122 kb on + strand, within CCNA_00563 at 578.134 kb on + strand, within CCNA_00563 at 578.135 kb on + strand, within CCNA_00563 at 578.142 kb on - strand, within CCNA_00563 at 578.153 kb on + strand, within CCNA_00563 at 578.161 kb on - strand, within CCNA_00563 at 578.164 kb on + strand, within CCNA_00563 at 578.172 kb on - strand, within CCNA_00563 at 578.213 kb on + strand, within CCNA_00563 at 578.214 kb on + strand, within CCNA_00563 at 578.221 kb on + strand, within CCNA_00563 at 578.221 kb on + strand, within CCNA_00563 at 578.236 kb on - strand, within CCNA_00563 at 578.236 kb on - strand, within CCNA_00563 at 578.240 kb on + strand, within CCNA_00563 at 578.240 kb on + strand, within CCNA_00563 at 578.248 kb on - strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.293 kb on + strand, within CCNA_00563 at 578.296 kb on - strand, within CCNA_00563 at 578.296 kb on - strand, within CCNA_00563 at 578.301 kb on - strand, within CCNA_00563 at 578.301 kb on - strand, within CCNA_00563 at 578.301 kb on - strand, within CCNA_00563 at 578.307 kb on - strand, within CCNA_00563 at 578.307 kb on - strand at 578.307 kb on - strand, within CCNA_00563 at 578.357 kb on + strand, within CCNA_00563
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.03 mg/ml remove 575,674 - +0.2 575,739 - -1.0 575,741 - +0.9 575,749 - +1.1 575,797 - -0.3 575,799 + -2.3 575,801 + -0.0 575,801 + -0.2 575,801 + +1.6 575,801 + -0.3 575,801 + +0.6 575,801 + -0.3 575,807 - -2.2 575,807 - +0.7 575,809 - -0.6 575,818 + +1.9 575,835 - +0.4 575,841 + +1.2 575,847 + -0.8 575,847 + -0.5 575,860 + +0.7 575,860 + -0.4 575,868 - CCNA_00560 0.10 -1.8 575,868 - CCNA_00560 0.10 +0.4 575,898 + CCNA_00560 0.16 +1.0 575,900 - CCNA_00560 0.17 +0.4 576,001 + CCNA_00560 0.36 +0.3 576,027 - CCNA_00560 0.41 +0.3 576,031 - CCNA_00560 0.42 +1.6 576,048 + CCNA_00560 0.45 +1.1 576,057 + CCNA_00560 0.47 -1.4 576,057 + CCNA_00560 0.47 -0.1 576,057 + CCNA_00560 0.47 +0.7 576,057 + CCNA_00560 0.47 +1.2 576,065 - CCNA_00560 0.49 -0.3 576,175 + CCNA_00560 0.70 +0.3 576,175 + CCNA_00560 0.70 +0.3 576,188 - CCNA_00560 0.73 +0.4 576,191 + CCNA_00560 0.73 -0.5 576,244 + CCNA_00560 0.84 +0.8 576,263 - CCNA_00560 0.87 -0.8 576,270 - CCNA_00560 0.89 +0.5 576,288 + +0.2 576,288 + +1.3 576,340 - +0.4 576,342 + -1.6 576,342 + +0.5 576,342 + +0.2 576,342 + +0.3 576,350 - -1.8 576,350 - +0.7 576,357 + +1.4 576,357 + +1.1 576,375 + CCNA_00561 0.15 +0.8 576,375 + CCNA_00561 0.15 +0.4 576,383 - CCNA_00561 0.17 +1.6 576,383 - CCNA_00561 0.17 +1.6 576,383 - CCNA_00561 0.17 -1.0 576,383 - CCNA_00561 0.17 +0.2 576,431 - CCNA_00561 0.31 +1.1 576,455 - CCNA_00561 0.38 +0.7 576,455 - CCNA_00561 0.38 +0.3 576,470 + CCNA_00561 0.42 +1.2 576,478 - CCNA_00561 0.45 +0.6 576,528 + CCNA_00561 0.59 -0.0 576,536 - CCNA_00561 0.61 +0.9 576,536 - +1.1 576,536 - CCNA_00561 0.61 -0.5 576,580 - CCNA_00561 0.74 -0.8 576,600 - CCNA_00561 0.80 -1.2 576,615 + CCNA_00561 0.84 +1.1 576,669 + +1.8 576,695 + +2.5 576,695 + +2.3 576,703 - +0.6 576,703 - +1.1 576,703 - +2.2 576,727 - +0.3 576,742 + +2.1 576,747 + +1.8 576,750 - +1.8 576,761 - -0.9 576,761 - +0.9 576,761 - -0.4 576,783 + CCNA_00562 0.11 +1.5 576,810 + CCNA_00562 0.14 +1.3 576,818 - CCNA_00562 0.14 +1.9 576,841 - CCNA_00562 0.17 +0.6 576,890 + CCNA_00562 0.21 +2.7 576,905 - CCNA_00562 0.23 +2.1 576,915 + CCNA_00562 0.24 +1.9 576,983 + CCNA_00562 0.30 +0.9 576,998 + CCNA_00562 0.31 +2.0 577,006 - CCNA_00562 0.32 +1.0 577,006 - CCNA_00562 0.32 +0.6 577,054 - CCNA_00562 0.37 +1.6 577,072 - CCNA_00562 0.38 +1.9 577,087 - CCNA_00562 0.40 +0.8 577,097 - CCNA_00562 0.41 -0.3 577,148 + CCNA_00562 0.46 +2.0 577,188 + CCNA_00562 0.49 +1.3 577,188 + CCNA_00562 0.49 +1.3 577,196 - CCNA_00562 0.50 -1.3 577,196 - CCNA_00562 0.50 -0.9 577,205 + CCNA_00562 0.51 +2.0 577,210 + CCNA_00562 0.51 +2.6 577,210 + CCNA_00562 0.51 +1.3 577,210 + CCNA_00562 0.51 +0.7 577,210 + CCNA_00562 0.51 +1.4 577,210 + CCNA_00562 0.51 +1.5 577,210 + CCNA_00562 0.51 +0.3 577,218 - CCNA_00562 0.52 -0.3 577,218 - CCNA_00562 0.52 -0.3 577,262 + CCNA_00562 0.56 +2.2 577,270 - CCNA_00562 0.57 +1.6 577,272 - CCNA_00562 0.57 +0.3 577,311 + CCNA_00562 0.61 +2.3 577,319 - CCNA_00562 0.62 +1.6 577,333 + CCNA_00562 0.63 +3.9 577,333 + CCNA_00562 0.63 +2.2 577,342 - CCNA_00562 0.64 -1.0 577,342 - CCNA_00562 0.64 +0.4 577,347 - CCNA_00562 0.64 +2.3 577,357 + CCNA_00562 0.65 +0.6 577,369 + CCNA_00562 0.66 +1.9 577,370 - CCNA_00562 0.67 +0.4 577,385 - CCNA_00562 0.68 +0.4 577,405 - CCNA_00562 0.70 +1.3 577,439 - CCNA_00562 0.73 -1.1 577,474 + CCNA_00562 0.76 +3.5 577,526 + CCNA_00562 0.81 +2.1 577,571 + CCNA_00562 0.86 +1.4 577,598 + CCNA_00562 0.88 +1.8 577,603 + CCNA_00562 0.89 +1.6 577,603 + CCNA_00562 0.89 +2.2 577,606 - CCNA_00562 0.89 -0.6 577,608 + CCNA_00562 0.89 +2.0 577,613 + CCNA_00562 0.90 +0.7 577,616 - CCNA_00562 0.90 +1.8 577,618 + +2.0 577,626 - +0.6 577,629 + -1.5 577,634 + +3.1 577,702 - -0.2 577,726 - +0.7 577,732 - +0.2 577,735 + +0.6 577,735 + +1.3 577,735 + +2.3 577,743 - -1.1 577,754 + +0.8 577,773 + +0.3 577,793 - +0.0 577,887 + CCNA_00563 0.23 +1.3 577,887 + CCNA_00563 0.23 -1.8 577,887 + CCNA_00563 0.23 -0.3 577,895 - CCNA_00563 0.24 -0.2 577,899 + CCNA_00563 0.24 -1.7 577,957 - CCNA_00563 0.32 +2.5 578,000 + CCNA_00563 0.38 +2.2 578,035 + CCNA_00563 0.43 +0.1 578,043 - CCNA_00563 0.44 +0.3 578,082 - CCNA_00563 0.49 +1.3 578,082 - CCNA_00563 0.49 +2.5 578,082 - CCNA_00563 0.49 +1.5 578,122 + CCNA_00563 0.54 +1.2 578,134 + CCNA_00563 0.56 +0.3 578,135 + CCNA_00563 0.56 +0.4 578,142 - CCNA_00563 0.57 +0.3 578,153 + CCNA_00563 0.59 -0.0 578,161 - CCNA_00563 0.60 +0.6 578,164 + CCNA_00563 0.60 +0.7 578,172 - CCNA_00563 0.61 -0.6 578,213 + CCNA_00563 0.67 +0.8 578,214 + CCNA_00563 0.67 +0.8 578,221 + CCNA_00563 0.68 +1.3 578,221 + CCNA_00563 0.68 -1.9 578,236 - CCNA_00563 0.70 -1.1 578,236 - CCNA_00563 0.70 -1.4 578,240 + CCNA_00563 0.70 +0.5 578,240 + CCNA_00563 0.70 +0.0 578,248 - CCNA_00563 0.72 -0.8 578,293 + CCNA_00563 0.78 -1.6 578,293 + CCNA_00563 0.78 +2.0 578,293 + CCNA_00563 0.78 -2.1 578,293 + CCNA_00563 0.78 +1.5 578,293 + CCNA_00563 0.78 +1.7 578,296 - CCNA_00563 0.78 -0.1 578,296 - CCNA_00563 0.78 -1.3 578,301 - CCNA_00563 0.79 -0.5 578,301 - CCNA_00563 0.79 +0.0 578,301 - CCNA_00563 0.79 +1.1 578,307 - CCNA_00563 0.80 -0.2 578,307 - +1.9 578,307 - CCNA_00563 0.80 +0.1 578,357 + CCNA_00563 0.86 -0.4
Or see this region's nucleotide sequence