Experiment: Recovery broth with Ticarcillin 4e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18460 and BBR_RS18465 are separated by 408 nucleotides BBR_RS18465 and BBR_RS21045 are separated by 70 nucleotides BBR_RS21045 and BBR_RS18470 are separated by 31 nucleotides
BBR_RS18460: BBR_RS18460 - site-specific integrase, at 1,926,031 to 1,927,395
_RS18460
BBR_RS18465: BBR_RS18465 - integrase, at 1,927,804 to 1,928,067
_RS18465
BBR_RS21045: BBR_RS21045 - hypothetical protein, at 1,928,138 to 1,928,536
_RS21045
BBR_RS18470: BBR_RS18470 - MFS transporter, at 1,928,568 to 1,930,049
_RS18470
Position (kb)
1928
1929 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1927.163 kb on + strand, within BBR_RS18460 at 1927.163 kb on + strand, within BBR_RS18460 at 1927.163 kb on + strand, within BBR_RS18460 at 1927.163 kb on + strand, within BBR_RS18460 at 1927.171 kb on - strand, within BBR_RS18460 at 1927.215 kb on - strand, within BBR_RS18460 at 1927.215 kb on - strand, within BBR_RS18460 at 1927.251 kb on - strand, within BBR_RS18460 at 1927.251 kb on - strand, within BBR_RS18460 at 1927.261 kb on - strand at 1927.263 kb on + strand at 1927.296 kb on + strand at 1927.296 kb on + strand at 1927.303 kb on - strand at 1927.360 kb on - strand at 1927.413 kb on - strand at 1927.435 kb on + strand at 1927.469 kb on + strand at 1927.477 kb on - strand at 1927.477 kb on - strand at 1927.477 kb on - strand at 1927.518 kb on + strand at 1927.551 kb on + strand at 1927.590 kb on + strand at 1927.593 kb on - strand at 1927.593 kb on - strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.595 kb on + strand at 1927.600 kb on + strand at 1927.603 kb on - strand at 1927.603 kb on - strand at 1927.607 kb on + strand at 1927.608 kb on - strand at 1927.623 kb on + strand at 1927.623 kb on + strand at 1927.646 kb on - strand at 1927.646 kb on - strand at 1927.697 kb on + strand at 1927.697 kb on + strand at 1927.705 kb on + strand at 1927.705 kb on - strand at 1927.710 kb on + strand at 1927.813 kb on + strand at 1927.828 kb on + strand at 1927.828 kb on + strand at 1927.847 kb on - strand, within BBR_RS18465 at 1927.857 kb on - strand, within BBR_RS18465 at 1927.859 kb on + strand, within BBR_RS18465 at 1927.867 kb on - strand, within BBR_RS18465 at 1927.877 kb on - strand, within BBR_RS18465 at 1927.877 kb on - strand, within BBR_RS18465 at 1927.898 kb on + strand, within BBR_RS18465 at 1927.898 kb on + strand, within BBR_RS18465 at 1927.900 kb on + strand, within BBR_RS18465 at 1927.906 kb on + strand, within BBR_RS18465 at 1927.906 kb on + strand, within BBR_RS18465 at 1927.906 kb on - strand, within BBR_RS18465 at 1927.906 kb on - strand, within BBR_RS18465 at 1927.906 kb on - strand, within BBR_RS18465 at 1927.908 kb on - strand, within BBR_RS18465 at 1927.936 kb on - strand, within BBR_RS18465 at 1927.936 kb on - strand, within BBR_RS18465 at 1927.967 kb on + strand, within BBR_RS18465 at 1927.975 kb on + strand, within BBR_RS18465 at 1927.982 kb on - strand, within BBR_RS18465 at 1927.982 kb on - strand, within BBR_RS18465 at 1927.982 kb on - strand, within BBR_RS18465 at 1927.983 kb on + strand, within BBR_RS18465 at 1927.989 kb on + strand, within BBR_RS18465 at 1927.991 kb on - strand, within BBR_RS18465 at 1928.003 kb on + strand, within BBR_RS18465 at 1928.009 kb on + strand, within BBR_RS18465 at 1928.010 kb on - strand, within BBR_RS18465 at 1928.017 kb on - strand, within BBR_RS18465 at 1928.017 kb on - strand, within BBR_RS18465 at 1928.031 kb on + strand, within BBR_RS18465 at 1928.107 kb on + strand at 1928.113 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.115 kb on - strand at 1928.137 kb on + strand at 1928.145 kb on - strand at 1928.150 kb on - strand at 1928.179 kb on + strand, within BBR_RS21045 at 1928.182 kb on - strand, within BBR_RS21045 at 1928.182 kb on - strand, within BBR_RS21045 at 1928.223 kb on - strand, within BBR_RS21045 at 1928.229 kb on - strand, within BBR_RS21045 at 1928.247 kb on + strand, within BBR_RS21045 at 1928.311 kb on - strand, within BBR_RS21045 at 1928.311 kb on - strand, within BBR_RS21045 at 1928.319 kb on - strand, within BBR_RS21045 at 1928.326 kb on + strand, within BBR_RS21045 at 1928.405 kb on + strand, within BBR_RS21045 at 1928.408 kb on - strand, within BBR_RS21045 at 1928.502 kb on + strand at 1928.537 kb on + strand at 1928.557 kb on + strand at 1928.565 kb on - strand at 1928.574 kb on - strand at 1928.702 kb on + strand at 1928.704 kb on - strand at 1928.721 kb on - strand, within BBR_RS18470 at 1928.722 kb on + strand, within BBR_RS18470 at 1928.803 kb on + strand, within BBR_RS18470 at 1928.803 kb on + strand, within BBR_RS18470 at 1928.803 kb on + strand, within BBR_RS18470 at 1928.821 kb on - strand, within BBR_RS18470 at 1928.841 kb on - strand, within BBR_RS18470 at 1928.848 kb on + strand, within BBR_RS18470 at 1928.860 kb on - strand, within BBR_RS18470 at 1928.886 kb on - strand, within BBR_RS18470 at 1928.936 kb on - strand, within BBR_RS18470 at 1928.938 kb on - strand, within BBR_RS18470 at 1928.960 kb on - strand, within BBR_RS18470 at 1929.009 kb on - strand, within BBR_RS18470 at 1929.073 kb on + strand, within BBR_RS18470 at 1929.085 kb on + strand, within BBR_RS18470 at 1929.085 kb on + strand, within BBR_RS18470 at 1929.085 kb on + strand, within BBR_RS18470 at 1929.093 kb on - strand, within BBR_RS18470 at 1929.093 kb on - strand, within BBR_RS18470 at 1929.105 kb on - strand, within BBR_RS18470 at 1929.105 kb on - strand, within BBR_RS18470 at 1929.115 kb on - strand, within BBR_RS18470 at 1929.214 kb on + strand, within BBR_RS18470 at 1929.219 kb on - strand, within BBR_RS18470 at 1929.233 kb on + strand, within BBR_RS18470 at 1929.246 kb on - strand, within BBR_RS18470 at 1929.246 kb on - strand, within BBR_RS18470 at 1929.268 kb on + strand, within BBR_RS18470 at 1929.288 kb on - strand, within BBR_RS18470 at 1929.292 kb on + strand, within BBR_RS18470 at 1929.300 kb on - strand, within BBR_RS18470 at 1929.414 kb on + strand, within BBR_RS18470 at 1929.529 kb on - strand, within BBR_RS18470 at 1929.531 kb on - strand, within BBR_RS18470
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 4e-04 mM remove 1,927,163 + BBR_RS18460 0.83 -0.5 1,927,163 + BBR_RS18460 0.83 +1.2 1,927,163 + BBR_RS18460 0.83 +1.1 1,927,163 + BBR_RS18460 0.83 +1.4 1,927,171 - BBR_RS18460 0.84 -0.3 1,927,215 - BBR_RS18460 0.87 +0.8 1,927,215 - BBR_RS18460 0.87 -2.2 1,927,251 - BBR_RS18460 0.89 +2.7 1,927,251 - BBR_RS18460 0.89 +0.5 1,927,261 - -0.2 1,927,263 + +0.4 1,927,296 + -0.4 1,927,296 + -0.4 1,927,303 - -0.1 1,927,360 - +0.0 1,927,413 - -0.5 1,927,435 + +0.7 1,927,469 + +0.5 1,927,477 - -0.1 1,927,477 - +0.6 1,927,477 - -1.6 1,927,518 + +2.6 1,927,551 + +0.5 1,927,590 + +0.7 1,927,593 - +0.4 1,927,593 - +0.4 1,927,595 + -0.2 1,927,595 + -0.1 1,927,595 + -0.5 1,927,595 + +0.2 1,927,595 + -0.2 1,927,595 + +0.2 1,927,595 + -0.3 1,927,600 + +2.2 1,927,603 - -2.3 1,927,603 - +1.7 1,927,607 + -0.5 1,927,608 - -1.0 1,927,623 + +1.2 1,927,623 + +0.4 1,927,646 - +0.3 1,927,646 - +1.7 1,927,697 + +0.4 1,927,697 + -0.1 1,927,705 + +0.2 1,927,705 - +0.8 1,927,710 + -0.3 1,927,813 + +0.4 1,927,828 + +0.4 1,927,828 + +0.8 1,927,847 - BBR_RS18465 0.16 -0.1 1,927,857 - BBR_RS18465 0.20 -0.8 1,927,859 + BBR_RS18465 0.21 +0.3 1,927,867 - BBR_RS18465 0.24 +0.1 1,927,877 - BBR_RS18465 0.28 +1.5 1,927,877 - BBR_RS18465 0.28 -1.6 1,927,898 + BBR_RS18465 0.36 +1.2 1,927,898 + BBR_RS18465 0.36 +1.0 1,927,900 + BBR_RS18465 0.36 -0.9 1,927,906 + BBR_RS18465 0.39 -0.9 1,927,906 + BBR_RS18465 0.39 +0.1 1,927,906 - BBR_RS18465 0.39 +0.7 1,927,906 - BBR_RS18465 0.39 +1.0 1,927,906 - BBR_RS18465 0.39 -0.9 1,927,908 - BBR_RS18465 0.39 -1.3 1,927,936 - BBR_RS18465 0.50 -1.8 1,927,936 - BBR_RS18465 0.50 -0.2 1,927,967 + BBR_RS18465 0.62 -0.4 1,927,975 + BBR_RS18465 0.65 +0.8 1,927,982 - BBR_RS18465 0.67 +0.2 1,927,982 - BBR_RS18465 0.67 +1.9 1,927,982 - BBR_RS18465 0.67 +0.4 1,927,983 + BBR_RS18465 0.68 -1.5 1,927,989 + BBR_RS18465 0.70 +0.3 1,927,991 - BBR_RS18465 0.71 +0.6 1,928,003 + BBR_RS18465 0.75 -0.2 1,928,009 + BBR_RS18465 0.78 +1.4 1,928,010 - BBR_RS18465 0.78 +0.0 1,928,017 - BBR_RS18465 0.81 +0.6 1,928,017 - BBR_RS18465 0.81 -0.4 1,928,031 + BBR_RS18465 0.86 +3.1 1,928,107 + +2.8 1,928,113 - +1.2 1,928,115 - -0.4 1,928,115 - +1.1 1,928,115 - +1.0 1,928,115 - -0.9 1,928,115 - +0.2 1,928,115 - +0.7 1,928,115 - -0.2 1,928,115 - -1.8 1,928,115 - +0.7 1,928,137 + +0.5 1,928,145 - -0.1 1,928,150 - +1.7 1,928,179 + BBR_RS21045 0.10 +0.5 1,928,182 - BBR_RS21045 0.11 +1.7 1,928,182 - BBR_RS21045 0.11 +2.2 1,928,223 - BBR_RS21045 0.21 -1.0 1,928,229 - BBR_RS21045 0.23 -0.1 1,928,247 + BBR_RS21045 0.27 +1.5 1,928,311 - BBR_RS21045 0.43 +1.6 1,928,311 - BBR_RS21045 0.43 +1.9 1,928,319 - BBR_RS21045 0.45 -1.0 1,928,326 + BBR_RS21045 0.47 +0.1 1,928,405 + BBR_RS21045 0.67 -1.3 1,928,408 - BBR_RS21045 0.68 +0.6 1,928,502 + -0.7 1,928,537 + +1.8 1,928,557 + +0.0 1,928,565 - -0.8 1,928,574 - +0.9 1,928,702 + +0.8 1,928,704 - +0.5 1,928,721 - BBR_RS18470 0.10 +1.1 1,928,722 + BBR_RS18470 0.10 +0.7 1,928,803 + BBR_RS18470 0.16 +0.9 1,928,803 + BBR_RS18470 0.16 +0.8 1,928,803 + BBR_RS18470 0.16 +1.1 1,928,821 - BBR_RS18470 0.17 -0.3 1,928,841 - BBR_RS18470 0.18 +0.1 1,928,848 + BBR_RS18470 0.19 +0.8 1,928,860 - BBR_RS18470 0.20 -0.2 1,928,886 - BBR_RS18470 0.21 +1.9 1,928,936 - BBR_RS18470 0.25 +0.9 1,928,938 - BBR_RS18470 0.25 +0.9 1,928,960 - BBR_RS18470 0.26 +1.2 1,929,009 - BBR_RS18470 0.30 +1.1 1,929,073 + BBR_RS18470 0.34 +1.2 1,929,085 + BBR_RS18470 0.35 -0.9 1,929,085 + BBR_RS18470 0.35 -0.5 1,929,085 + BBR_RS18470 0.35 +0.4 1,929,093 - BBR_RS18470 0.35 +1.2 1,929,093 - BBR_RS18470 0.35 -0.2 1,929,105 - BBR_RS18470 0.36 +2.6 1,929,105 - BBR_RS18470 0.36 +2.4 1,929,115 - BBR_RS18470 0.37 +1.0 1,929,214 + BBR_RS18470 0.44 +2.9 1,929,219 - BBR_RS18470 0.44 +0.7 1,929,233 + BBR_RS18470 0.45 +1.1 1,929,246 - BBR_RS18470 0.46 +3.6 1,929,246 - BBR_RS18470 0.46 +1.2 1,929,268 + BBR_RS18470 0.47 +0.9 1,929,288 - BBR_RS18470 0.49 +1.2 1,929,292 + BBR_RS18470 0.49 -0.2 1,929,300 - BBR_RS18470 0.49 +0.4 1,929,414 + BBR_RS18470 0.57 +1.0 1,929,529 - BBR_RS18470 0.65 +0.8 1,929,531 - BBR_RS18470 0.65 -1.6
Or see this region's nucleotide sequence