Experiment: Recovery broth with Ticarcillin 4e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17310 and BBR_RS17315 are separated by 251 nucleotides BBR_RS17315 and BBR_RS17320 are separated by 3 nucleotides BBR_RS17320 and BBR_RS17330 are separated by 174 nucleotides
BBR_RS17310: BBR_RS17310 - D-2-hydroxyacid dehydrogenase, at 1,660,687 to 1,661,673
_RS17310
BBR_RS17315: BBR_RS17315 - MFS transporter, at 1,661,925 to 1,663,385
_RS17315
BBR_RS17320: BBR_RS17320 - response regulator, at 1,663,389 to 1,663,853
_RS17320
BBR_RS17330: BBR_RS17330 - replication-associated recombination protein A, at 1,664,028 to 1,665,407
_RS17330
Position (kb)
1661
1662
1663
1664 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 1660.936 kb on + strand, within BBR_RS17310 at 1660.954 kb on + strand, within BBR_RS17310 at 1660.975 kb on - strand, within BBR_RS17310 at 1660.987 kb on - strand, within BBR_RS17310 at 1661.008 kb on - strand, within BBR_RS17310 at 1661.014 kb on + strand, within BBR_RS17310 at 1661.014 kb on + strand, within BBR_RS17310 at 1661.015 kb on - strand, within BBR_RS17310 at 1661.022 kb on - strand, within BBR_RS17310 at 1661.069 kb on + strand, within BBR_RS17310 at 1661.153 kb on + strand, within BBR_RS17310 at 1661.153 kb on + strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.161 kb on - strand, within BBR_RS17310 at 1661.163 kb on + strand, within BBR_RS17310 at 1661.173 kb on + strand, within BBR_RS17310 at 1661.173 kb on + strand, within BBR_RS17310 at 1661.188 kb on + strand, within BBR_RS17310 at 1661.209 kb on - strand, within BBR_RS17310 at 1661.219 kb on - strand, within BBR_RS17310 at 1661.219 kb on - strand, within BBR_RS17310 at 1661.223 kb on + strand, within BBR_RS17310 at 1661.223 kb on + strand, within BBR_RS17310 at 1661.223 kb on + strand, within BBR_RS17310 at 1661.235 kb on + strand, within BBR_RS17310 at 1661.235 kb on + strand, within BBR_RS17310 at 1661.243 kb on - strand, within BBR_RS17310 at 1661.251 kb on - strand, within BBR_RS17310 at 1661.255 kb on - strand, within BBR_RS17310 at 1661.279 kb on - strand, within BBR_RS17310 at 1661.279 kb on - strand, within BBR_RS17310 at 1661.306 kb on + strand, within BBR_RS17310 at 1661.314 kb on - strand, within BBR_RS17310 at 1661.383 kb on + strand, within BBR_RS17310 at 1661.401 kb on + strand, within BBR_RS17310 at 1661.410 kb on + strand, within BBR_RS17310 at 1661.417 kb on - strand, within BBR_RS17310 at 1661.455 kb on - strand, within BBR_RS17310 at 1661.457 kb on + strand, within BBR_RS17310 at 1661.460 kb on - strand, within BBR_RS17310 at 1661.505 kb on - strand, within BBR_RS17310 at 1661.544 kb on + strand, within BBR_RS17310 at 1661.594 kb on + strand at 1661.722 kb on + strand at 1661.731 kb on - strand at 1661.731 kb on - strand at 1661.731 kb on - strand at 1661.746 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on + strand at 1661.753 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.761 kb on - strand at 1661.785 kb on + strand at 1661.796 kb on - strand at 1661.796 kb on - strand at 1661.859 kb on + strand at 1661.876 kb on - strand at 1661.882 kb on + strand at 1661.929 kb on - strand at 1661.939 kb on - strand at 1661.972 kb on - strand at 1662.036 kb on + strand at 1662.038 kb on + strand at 1662.066 kb on + strand at 1662.080 kb on - strand, within BBR_RS17315 at 1662.208 kb on + strand, within BBR_RS17315 at 1662.245 kb on + strand, within BBR_RS17315 at 1662.253 kb on - strand, within BBR_RS17315 at 1662.283 kb on - strand, within BBR_RS17315 at 1662.312 kb on + strand, within BBR_RS17315 at 1662.323 kb on + strand, within BBR_RS17315 at 1662.368 kb on + strand, within BBR_RS17315 at 1662.376 kb on - strand, within BBR_RS17315 at 1662.411 kb on + strand, within BBR_RS17315 at 1662.499 kb on - strand, within BBR_RS17315 at 1662.499 kb on - strand, within BBR_RS17315 at 1662.532 kb on - strand, within BBR_RS17315 at 1662.558 kb on + strand, within BBR_RS17315 at 1662.574 kb on + strand, within BBR_RS17315 at 1662.574 kb on + strand, within BBR_RS17315 at 1662.594 kb on - strand, within BBR_RS17315 at 1662.729 kb on + strand, within BBR_RS17315 at 1662.735 kb on - strand, within BBR_RS17315 at 1662.735 kb on - strand, within BBR_RS17315 at 1662.770 kb on + strand, within BBR_RS17315 at 1662.813 kb on + strand, within BBR_RS17315 at 1662.820 kb on + strand, within BBR_RS17315 at 1662.820 kb on + strand, within BBR_RS17315 at 1662.880 kb on + strand, within BBR_RS17315 at 1662.890 kb on + strand, within BBR_RS17315 at 1662.897 kb on + strand, within BBR_RS17315 at 1662.903 kb on - strand, within BBR_RS17315 at 1662.910 kb on - strand, within BBR_RS17315 at 1662.991 kb on + strand, within BBR_RS17315 at 1662.997 kb on + strand, within BBR_RS17315 at 1663.021 kb on - strand, within BBR_RS17315 at 1663.021 kb on - strand, within BBR_RS17315 at 1663.028 kb on + strand, within BBR_RS17315 at 1663.041 kb on - strand, within BBR_RS17315 at 1663.073 kb on + strand, within BBR_RS17315 at 1663.081 kb on - strand, within BBR_RS17315 at 1663.081 kb on - strand, within BBR_RS17315 at 1663.088 kb on - strand, within BBR_RS17315 at 1663.115 kb on + strand, within BBR_RS17315 at 1663.126 kb on + strand, within BBR_RS17315 at 1663.168 kb on + strand, within BBR_RS17315 at 1663.168 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.197 kb on + strand, within BBR_RS17315 at 1663.224 kb on + strand, within BBR_RS17315 at 1663.232 kb on - strand, within BBR_RS17315 at 1663.255 kb on - strand at 1663.307 kb on + strand at 1663.360 kb on - strand at 1663.458 kb on - strand, within BBR_RS17320 at 1663.508 kb on + strand, within BBR_RS17320 at 1663.517 kb on - strand, within BBR_RS17320 at 1663.518 kb on + strand, within BBR_RS17320 at 1663.526 kb on - strand, within BBR_RS17320 at 1663.527 kb on + strand, within BBR_RS17320 at 1663.564 kb on + strand, within BBR_RS17320 at 1663.646 kb on - strand, within BBR_RS17320 at 1663.646 kb on - strand, within BBR_RS17320 at 1663.649 kb on - strand, within BBR_RS17320 at 1663.671 kb on - strand, within BBR_RS17320 at 1663.676 kb on - strand, within BBR_RS17320 at 1663.676 kb on - strand, within BBR_RS17320 at 1663.732 kb on + strand, within BBR_RS17320 at 1663.732 kb on + strand, within BBR_RS17320 at 1663.740 kb on + strand, within BBR_RS17320 at 1663.740 kb on - strand, within BBR_RS17320 at 1663.762 kb on - strand, within BBR_RS17320 at 1663.792 kb on - strand, within BBR_RS17320 at 1663.792 kb on - strand, within BBR_RS17320 at 1663.792 kb on - strand, within BBR_RS17320 at 1663.802 kb on - strand, within BBR_RS17320 at 1663.802 kb on - strand, within BBR_RS17320 at 1663.808 kb on + strand at 1663.833 kb on - strand at 1663.837 kb on - strand at 1663.864 kb on + strand at 1663.864 kb on + strand at 1663.894 kb on - strand at 1663.923 kb on - strand at 1663.941 kb on - strand at 1663.970 kb on + strand at 1663.973 kb on - strand at 1664.019 kb on + strand at 1664.020 kb on - strand at 1664.022 kb on - strand at 1664.057 kb on - strand at 1664.067 kb on + strand at 1664.136 kb on + strand at 1664.196 kb on + strand, within BBR_RS17330 at 1664.210 kb on - strand, within BBR_RS17330 at 1664.212 kb on - strand, within BBR_RS17330 at 1664.222 kb on - strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.256 kb on + strand, within BBR_RS17330 at 1664.323 kb on + strand, within BBR_RS17330 at 1664.331 kb on - strand, within BBR_RS17330 at 1664.338 kb on + strand, within BBR_RS17330
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 4e-04 mM remove 1,660,936 + BBR_RS17310 0.25 -0.9 1,660,954 + BBR_RS17310 0.27 -0.7 1,660,975 - BBR_RS17310 0.29 -1.7 1,660,987 - BBR_RS17310 0.30 +1.2 1,661,008 - BBR_RS17310 0.33 +1.8 1,661,014 + BBR_RS17310 0.33 +0.7 1,661,014 + BBR_RS17310 0.33 -1.1 1,661,015 - BBR_RS17310 0.33 +1.1 1,661,022 - BBR_RS17310 0.34 -0.6 1,661,069 + BBR_RS17310 0.39 -0.7 1,661,153 + BBR_RS17310 0.47 +1.0 1,661,153 + BBR_RS17310 0.47 +1.6 1,661,161 - BBR_RS17310 0.48 -0.3 1,661,161 - BBR_RS17310 0.48 +1.7 1,661,161 - BBR_RS17310 0.48 -0.3 1,661,161 - BBR_RS17310 0.48 +1.3 1,661,161 - BBR_RS17310 0.48 -0.2 1,661,161 - BBR_RS17310 0.48 +0.2 1,661,161 - BBR_RS17310 0.48 +0.7 1,661,163 + BBR_RS17310 0.48 +0.6 1,661,173 + BBR_RS17310 0.49 +0.1 1,661,173 + BBR_RS17310 0.49 -1.2 1,661,188 + BBR_RS17310 0.51 +1.4 1,661,209 - BBR_RS17310 0.53 +2.2 1,661,219 - BBR_RS17310 0.54 -0.4 1,661,219 - BBR_RS17310 0.54 +0.5 1,661,223 + BBR_RS17310 0.54 -0.3 1,661,223 + BBR_RS17310 0.54 -0.3 1,661,223 + BBR_RS17310 0.54 +0.2 1,661,235 + BBR_RS17310 0.56 +0.1 1,661,235 + BBR_RS17310 0.56 -0.3 1,661,243 - BBR_RS17310 0.56 +2.0 1,661,251 - BBR_RS17310 0.57 +0.6 1,661,255 - BBR_RS17310 0.58 +0.1 1,661,279 - BBR_RS17310 0.60 +0.1 1,661,279 - BBR_RS17310 0.60 -1.5 1,661,306 + BBR_RS17310 0.63 -1.2 1,661,314 - BBR_RS17310 0.64 -0.2 1,661,383 + BBR_RS17310 0.71 +3.0 1,661,401 + BBR_RS17310 0.72 -0.7 1,661,410 + BBR_RS17310 0.73 +1.5 1,661,417 - BBR_RS17310 0.74 +4.0 1,661,455 - BBR_RS17310 0.78 +0.8 1,661,457 + BBR_RS17310 0.78 -0.5 1,661,460 - BBR_RS17310 0.78 +0.5 1,661,505 - BBR_RS17310 0.83 +0.3 1,661,544 + BBR_RS17310 0.87 -1.3 1,661,594 + +0.7 1,661,722 + +0.3 1,661,731 - +0.4 1,661,731 - +0.4 1,661,731 - +1.5 1,661,746 + -0.6 1,661,753 + -1.8 1,661,753 + -0.3 1,661,753 + -1.0 1,661,753 + -0.8 1,661,753 + -1.3 1,661,753 + +0.1 1,661,753 - -0.7 1,661,761 - -0.4 1,661,761 - -0.2 1,661,761 - -0.6 1,661,761 - +0.2 1,661,761 - +0.6 1,661,761 - +0.5 1,661,785 + +1.4 1,661,796 - +2.4 1,661,796 - +1.3 1,661,859 + +1.6 1,661,876 - -2.4 1,661,882 + +1.5 1,661,929 - +4.7 1,661,939 - +1.4 1,661,972 - +0.6 1,662,036 + -0.9 1,662,038 + -0.4 1,662,066 + -1.4 1,662,080 - BBR_RS17315 0.11 +0.5 1,662,208 + BBR_RS17315 0.19 +1.2 1,662,245 + BBR_RS17315 0.22 -0.5 1,662,253 - BBR_RS17315 0.22 +0.8 1,662,283 - BBR_RS17315 0.25 -1.1 1,662,312 + BBR_RS17315 0.26 -0.1 1,662,323 + BBR_RS17315 0.27 +2.9 1,662,368 + BBR_RS17315 0.30 +0.1 1,662,376 - BBR_RS17315 0.31 -0.9 1,662,411 + BBR_RS17315 0.33 +0.3 1,662,499 - BBR_RS17315 0.39 +0.4 1,662,499 - BBR_RS17315 0.39 -0.9 1,662,532 - BBR_RS17315 0.42 +0.7 1,662,558 + BBR_RS17315 0.43 +3.4 1,662,574 + BBR_RS17315 0.44 +0.1 1,662,574 + BBR_RS17315 0.44 +0.7 1,662,594 - BBR_RS17315 0.46 -1.7 1,662,729 + BBR_RS17315 0.55 -0.8 1,662,735 - BBR_RS17315 0.55 +0.8 1,662,735 - BBR_RS17315 0.55 +0.4 1,662,770 + BBR_RS17315 0.58 +3.4 1,662,813 + BBR_RS17315 0.61 +0.1 1,662,820 + BBR_RS17315 0.61 -0.6 1,662,820 + BBR_RS17315 0.61 +0.2 1,662,880 + BBR_RS17315 0.65 +0.8 1,662,890 + BBR_RS17315 0.66 +0.3 1,662,897 + BBR_RS17315 0.67 +5.7 1,662,903 - BBR_RS17315 0.67 -1.0 1,662,910 - BBR_RS17315 0.67 -0.6 1,662,991 + BBR_RS17315 0.73 -0.3 1,662,997 + BBR_RS17315 0.73 +0.5 1,663,021 - BBR_RS17315 0.75 -1.4 1,663,021 - BBR_RS17315 0.75 -0.7 1,663,028 + BBR_RS17315 0.75 +0.6 1,663,041 - BBR_RS17315 0.76 +1.1 1,663,073 + BBR_RS17315 0.79 +1.3 1,663,081 - BBR_RS17315 0.79 -0.3 1,663,081 - BBR_RS17315 0.79 +0.0 1,663,088 - BBR_RS17315 0.80 +0.9 1,663,115 + BBR_RS17315 0.81 -0.1 1,663,126 + BBR_RS17315 0.82 +1.3 1,663,168 + BBR_RS17315 0.85 +0.1 1,663,168 + BBR_RS17315 0.85 +0.9 1,663,197 + BBR_RS17315 0.87 +0.3 1,663,197 + BBR_RS17315 0.87 -0.0 1,663,197 + BBR_RS17315 0.87 -0.7 1,663,197 + BBR_RS17315 0.87 +1.2 1,663,224 + BBR_RS17315 0.89 -0.0 1,663,232 - BBR_RS17315 0.89 +3.3 1,663,255 - +0.1 1,663,307 + +2.3 1,663,360 - +2.2 1,663,458 - BBR_RS17320 0.15 +0.1 1,663,508 + BBR_RS17320 0.26 +0.3 1,663,517 - BBR_RS17320 0.28 +0.5 1,663,518 + BBR_RS17320 0.28 +1.6 1,663,526 - BBR_RS17320 0.29 +1.3 1,663,527 + BBR_RS17320 0.30 -0.7 1,663,564 + BBR_RS17320 0.38 -0.0 1,663,646 - BBR_RS17320 0.55 -1.0 1,663,646 - BBR_RS17320 0.55 -1.3 1,663,649 - BBR_RS17320 0.56 +1.4 1,663,671 - BBR_RS17320 0.61 -2.4 1,663,676 - BBR_RS17320 0.62 -0.4 1,663,676 - BBR_RS17320 0.62 -0.0 1,663,732 + BBR_RS17320 0.74 +0.7 1,663,732 + BBR_RS17320 0.74 -0.4 1,663,740 + BBR_RS17320 0.75 +0.6 1,663,740 - BBR_RS17320 0.75 -0.4 1,663,762 - BBR_RS17320 0.80 -0.7 1,663,792 - BBR_RS17320 0.87 -1.5 1,663,792 - BBR_RS17320 0.87 -0.2 1,663,792 - BBR_RS17320 0.87 +2.4 1,663,802 - BBR_RS17320 0.89 -1.8 1,663,802 - BBR_RS17320 0.89 -0.5 1,663,808 + -0.7 1,663,833 - -1.3 1,663,837 - -0.7 1,663,864 + +1.1 1,663,864 + +0.7 1,663,894 - -2.0 1,663,923 - -0.4 1,663,941 - +3.6 1,663,970 + +1.5 1,663,973 - -0.8 1,664,019 + -0.8 1,664,020 - +0.5 1,664,022 - +0.8 1,664,057 - +6.8 1,664,067 + -0.2 1,664,136 + -0.6 1,664,196 + BBR_RS17330 0.12 -0.2 1,664,210 - BBR_RS17330 0.13 -1.0 1,664,212 - BBR_RS17330 0.13 -1.4 1,664,222 - BBR_RS17330 0.14 +1.9 1,664,256 + BBR_RS17330 0.17 +0.6 1,664,256 + BBR_RS17330 0.17 +5.2 1,664,256 + BBR_RS17330 0.17 +0.5 1,664,256 + BBR_RS17330 0.17 -0.0 1,664,323 + BBR_RS17330 0.21 -0.7 1,664,331 - BBR_RS17330 0.22 +0.9 1,664,338 + BBR_RS17330 0.22 -0.1
Or see this region's nucleotide sequence