Experiment: Recovery broth with Ticarcillin 4e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15865 and BBR_RS15870 are separated by 135 nucleotides BBR_RS15870 and BBR_RS20940 are separated by 28 nucleotides BBR_RS20940 and BBR_RS20945 overlap by 59 nucleotides BBR_RS20945 and BBR_RS15875 are separated by 31 nucleotides BBR_RS15875 and BBR_RS15880 are separated by 162 nucleotides
BBR_RS15865: BBR_RS15865 - GNAT family N-acetyltransferase, at 1,323,254 to 1,323,787
_RS15865
BBR_RS15870: BBR_RS15870 - hypothetical protein, at 1,323,923 to 1,324,252
_RS15870
BBR_RS20940: BBR_RS20940 - hypothetical protein, at 1,324,281 to 1,324,487
_RS20940
BBR_RS20945: BBR_RS20945 - hypothetical protein, at 1,324,429 to 1,324,630
_RS20945
BBR_RS15875: BBR_RS15875 - DNA-binding protein, at 1,324,662 to 1,325,468
_RS15875
BBR_RS15880: BBR_RS15880 - hypothetical protein, at 1,325,631 to 1,325,885
_RS15880
Position (kb)
1324
1325
1326 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1323.739 kb on + strand at 1323.771 kb on + strand at 1323.806 kb on + strand at 1323.814 kb on - strand at 1323.831 kb on - strand at 1323.852 kb on + strand at 1323.860 kb on - strand at 1323.881 kb on - strand at 1323.911 kb on + strand at 1323.919 kb on - strand at 1323.967 kb on - strand, within BBR_RS15870 at 1323.979 kb on - strand, within BBR_RS15870 at 1323.989 kb on + strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1324.029 kb on + strand, within BBR_RS15870 at 1324.159 kb on + strand, within BBR_RS15870 at 1324.205 kb on - strand, within BBR_RS15870 at 1324.247 kb on + strand at 1324.255 kb on - strand at 1324.269 kb on + strand at 1324.762 kb on + strand, within BBR_RS15875 at 1324.764 kb on + strand, within BBR_RS15875 at 1324.767 kb on + strand, within BBR_RS15875 at 1324.902 kb on + strand, within BBR_RS15875 at 1324.926 kb on + strand, within BBR_RS15875 at 1324.927 kb on - strand, within BBR_RS15875 at 1324.933 kb on - strand, within BBR_RS15875 at 1324.959 kb on - strand, within BBR_RS15875 at 1324.998 kb on + strand, within BBR_RS15875 at 1324.998 kb on + strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.006 kb on - strand, within BBR_RS15875 at 1325.011 kb on - strand, within BBR_RS15875 at 1325.011 kb on - strand, within BBR_RS15875 at 1325.026 kb on + strand, within BBR_RS15875 at 1325.035 kb on + strand, within BBR_RS15875 at 1325.081 kb on - strand, within BBR_RS15875 at 1325.116 kb on - strand, within BBR_RS15875 at 1325.132 kb on - strand, within BBR_RS15875 at 1325.202 kb on - strand, within BBR_RS15875 at 1325.232 kb on + strand, within BBR_RS15875 at 1325.303 kb on + strand, within BBR_RS15875 at 1325.389 kb on + strand at 1325.395 kb on + strand at 1325.418 kb on - strand at 1325.579 kb on + strand at 1325.634 kb on + strand at 1325.720 kb on - strand, within BBR_RS15880 at 1325.737 kb on + strand, within BBR_RS15880 at 1325.791 kb on + strand, within BBR_RS15880 at 1325.804 kb on - strand, within BBR_RS15880 at 1325.823 kb on + strand, within BBR_RS15880 at 1325.998 kb on + strand at 1326.000 kb on - strand at 1326.000 kb on - strand at 1326.021 kb on - strand at 1326.034 kb on + strand at 1326.132 kb on + strand at 1326.172 kb on - strand at 1326.172 kb on - strand at 1326.204 kb on - strand at 1326.412 kb on + strand at 1326.435 kb on + strand at 1326.459 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 4e-04 mM remove 1,323,739 + +0.8 1,323,771 + +1.6 1,323,806 + +0.5 1,323,814 - -0.3 1,323,831 - +0.1 1,323,852 + +2.1 1,323,860 - -2.1 1,323,881 - -1.5 1,323,911 + +1.6 1,323,919 - -0.6 1,323,967 - BBR_RS15870 0.13 +0.6 1,323,979 - BBR_RS15870 0.17 -0.2 1,323,989 + BBR_RS15870 0.20 -0.6 1,323,997 - BBR_RS15870 0.22 +2.2 1,323,997 - BBR_RS15870 0.22 +0.6 1,324,029 + BBR_RS15870 0.32 +0.6 1,324,159 + BBR_RS15870 0.72 +1.3 1,324,205 - BBR_RS15870 0.85 +1.1 1,324,247 + +1.2 1,324,255 - +0.7 1,324,269 + +1.1 1,324,762 + BBR_RS15875 0.12 +3.3 1,324,764 + BBR_RS15875 0.13 +0.3 1,324,767 + BBR_RS15875 0.13 -0.4 1,324,902 + BBR_RS15875 0.30 +1.6 1,324,926 + BBR_RS15875 0.33 -0.2 1,324,927 - BBR_RS15875 0.33 +0.2 1,324,933 - BBR_RS15875 0.34 +1.1 1,324,959 - BBR_RS15875 0.37 -0.0 1,324,998 + BBR_RS15875 0.42 +0.6 1,324,998 + BBR_RS15875 0.42 +1.8 1,325,006 - BBR_RS15875 0.43 +0.2 1,325,006 - BBR_RS15875 0.43 +1.2 1,325,006 - BBR_RS15875 0.43 +0.7 1,325,006 - BBR_RS15875 0.43 -0.8 1,325,006 - BBR_RS15875 0.43 +1.1 1,325,006 - BBR_RS15875 0.43 +0.7 1,325,011 - BBR_RS15875 0.43 +3.6 1,325,011 - BBR_RS15875 0.43 -0.9 1,325,026 + BBR_RS15875 0.45 +1.4 1,325,035 + BBR_RS15875 0.46 +0.3 1,325,081 - BBR_RS15875 0.52 -0.6 1,325,116 - BBR_RS15875 0.56 -1.8 1,325,132 - BBR_RS15875 0.58 -1.5 1,325,202 - BBR_RS15875 0.67 +1.7 1,325,232 + BBR_RS15875 0.71 +2.9 1,325,303 + BBR_RS15875 0.79 +0.2 1,325,389 + -0.3 1,325,395 + -0.8 1,325,418 - +0.4 1,325,579 + +1.2 1,325,634 + -0.9 1,325,720 - BBR_RS15880 0.35 +0.9 1,325,737 + BBR_RS15880 0.42 -0.1 1,325,791 + BBR_RS15880 0.63 -0.6 1,325,804 - BBR_RS15880 0.68 +0.5 1,325,823 + BBR_RS15880 0.75 -0.3 1,325,998 + +1.8 1,326,000 - +2.5 1,326,000 - -1.1 1,326,021 - +0.8 1,326,034 + +3.1 1,326,132 + +0.1 1,326,172 - -0.3 1,326,172 - -1.9 1,326,204 - +1.3 1,326,412 + -0.1 1,326,435 + +0.7 1,326,459 - +0.8
Or see this region's nucleotide sequence