Experiment: Recovery broth with Ticarcillin 4e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12715 and BBR_RS12720 are separated by 181 nucleotides BBR_RS12720 and BBR_RS12725 are separated by 140 nucleotides BBR_RS12725 and BBR_RS20515 are separated by 64 nucleotides
BBR_RS12715: BBR_RS12715 - helix-turn-helix domain-containing protein, at 607,399 to 607,782
_RS12715
BBR_RS12720: BBR_RS12720 - hypothetical protein, at 607,964 to 608,050
_RS12720
BBR_RS12725: BBR_RS12725 - hypothetical protein, at 608,191 to 608,571
_RS12725
BBR_RS20515: BBR_RS20515 - transposase, at 608,636 to 609,697
_RS20515
Position (kb) 607
608
609 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 606.971 kb on - strand at 606.972 kb on - strand at 607.017 kb on + strand at 607.025 kb on - strand at 607.084 kb on - strand at 607.085 kb on + strand at 607.108 kb on - strand at 607.117 kb on - strand at 607.123 kb on + strand at 607.123 kb on + strand at 607.123 kb on + strand at 607.123 kb on + strand at 607.131 kb on - strand at 607.145 kb on + strand at 607.147 kb on + strand at 607.179 kb on + strand at 607.211 kb on - strand at 607.216 kb on + strand at 607.224 kb on - strand at 607.229 kb on - strand at 607.256 kb on + strand at 607.256 kb on + strand at 607.305 kb on - strand at 607.310 kb on + strand at 607.401 kb on + strand at 607.401 kb on + strand at 607.409 kb on - strand at 607.432 kb on - strand at 607.437 kb on - strand at 607.437 kb on - strand at 607.442 kb on - strand, within BBR_RS12715 at 607.456 kb on - strand, within BBR_RS12715 at 607.468 kb on - strand, within BBR_RS12715 at 607.518 kb on + strand, within BBR_RS12715 at 607.540 kb on + strand, within BBR_RS12715 at 607.543 kb on + strand, within BBR_RS12715 at 607.577 kb on + strand, within BBR_RS12715 at 607.577 kb on + strand, within BBR_RS12715 at 607.597 kb on + strand, within BBR_RS12715 at 607.600 kb on - strand, within BBR_RS12715 at 607.600 kb on - strand, within BBR_RS12715 at 607.640 kb on + strand, within BBR_RS12715 at 607.653 kb on - strand, within BBR_RS12715 at 607.668 kb on + strand, within BBR_RS12715 at 607.676 kb on - strand, within BBR_RS12715 at 607.759 kb on - strand at 607.791 kb on + strand at 607.800 kb on - strand at 607.818 kb on + strand at 607.927 kb on - strand at 608.020 kb on - strand, within BBR_RS12720 at 608.021 kb on - strand, within BBR_RS12720 at 608.031 kb on - strand, within BBR_RS12720 at 608.117 kb on - strand at 608.117 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.123 kb on - strand at 608.152 kb on + strand at 608.181 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.191 kb on - strand at 608.193 kb on + strand at 608.201 kb on - strand at 608.201 kb on - strand at 608.211 kb on + strand at 608.231 kb on + strand, within BBR_RS12725 at 608.239 kb on - strand, within BBR_RS12725 at 608.347 kb on + strand, within BBR_RS12725 at 608.355 kb on - strand, within BBR_RS12725 at 608.357 kb on - strand, within BBR_RS12725 at 608.357 kb on - strand, within BBR_RS12725 at 608.379 kb on + strand, within BBR_RS12725 at 608.382 kb on - strand, within BBR_RS12725 at 608.397 kb on + strand, within BBR_RS12725 at 608.405 kb on - strand, within BBR_RS12725 at 608.424 kb on - strand, within BBR_RS12725 at 608.502 kb on - strand, within BBR_RS12725 at 608.553 kb on + strand at 608.555 kb on + strand at 608.555 kb on + strand at 608.555 kb on + strand at 608.561 kb on - strand at 608.563 kb on - strand at 608.563 kb on - strand at 608.599 kb on + strand at 608.602 kb on + strand at 608.604 kb on + strand at 608.662 kb on - strand at 608.663 kb on + strand at 608.663 kb on + strand at 608.671 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.676 kb on - strand at 608.749 kb on - strand, within BBR_RS20515 at 608.767 kb on + strand, within BBR_RS20515 at 608.767 kb on - strand, within BBR_RS20515 at 608.804 kb on - strand, within BBR_RS20515 at 608.841 kb on + strand, within BBR_RS20515 at 608.841 kb on + strand, within BBR_RS20515 at 608.864 kb on - strand, within BBR_RS20515 at 608.864 kb on - strand, within BBR_RS20515 at 608.866 kb on + strand, within BBR_RS20515 at 608.935 kb on + strand, within BBR_RS20515 at 608.942 kb on + strand, within BBR_RS20515 at 608.942 kb on + strand, within BBR_RS20515 at 608.950 kb on - strand, within BBR_RS20515 at 608.950 kb on - strand, within BBR_RS20515 at 608.960 kb on - strand, within BBR_RS20515 at 608.973 kb on + strand, within BBR_RS20515 at 608.973 kb on + strand, within BBR_RS20515 at 608.978 kb on + strand, within BBR_RS20515 at 608.981 kb on - strand, within BBR_RS20515 at 608.981 kb on - strand, within BBR_RS20515 at 608.981 kb on - strand, within BBR_RS20515 at 609.043 kb on - strand, within BBR_RS20515
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 4e-04 mM remove 606,971 - +0.2 606,972 - -0.6 607,017 + -0.5 607,025 - +0.7 607,084 - -1.3 607,085 + -0.2 607,108 - -0.4 607,117 - +0.7 607,123 + +2.7 607,123 + -1.3 607,123 + -1.2 607,123 + +1.0 607,131 - +0.6 607,145 + +1.8 607,147 + -0.0 607,179 + +0.1 607,211 - +0.6 607,216 + -0.2 607,224 - -0.2 607,229 - +0.9 607,256 + +1.0 607,256 + +1.0 607,305 - -0.1 607,310 + -0.7 607,401 + +0.3 607,401 + +2.1 607,409 - -0.5 607,432 - +0.0 607,437 - -1.5 607,437 - -0.4 607,442 - BBR_RS12715 0.11 +0.8 607,456 - BBR_RS12715 0.15 -0.8 607,468 - BBR_RS12715 0.18 +0.0 607,518 + BBR_RS12715 0.31 -0.1 607,540 + BBR_RS12715 0.37 -1.7 607,543 + BBR_RS12715 0.38 -0.7 607,577 + BBR_RS12715 0.46 +1.1 607,577 + BBR_RS12715 0.46 -0.2 607,597 + BBR_RS12715 0.52 -0.2 607,600 - BBR_RS12715 0.52 +1.8 607,600 - BBR_RS12715 0.52 +1.7 607,640 + BBR_RS12715 0.63 +0.3 607,653 - BBR_RS12715 0.66 +2.0 607,668 + BBR_RS12715 0.70 -0.9 607,676 - BBR_RS12715 0.72 +2.5 607,759 - +0.2 607,791 + +1.2 607,800 - -0.9 607,818 + -1.2 607,927 - +0.4 608,020 - BBR_RS12720 0.64 +3.5 608,021 - BBR_RS12720 0.66 +2.4 608,031 - BBR_RS12720 0.77 +0.7 608,117 - +0.8 608,117 - +0.1 608,123 - -0.0 608,123 - +2.2 608,123 - -0.7 608,123 - +0.5 608,152 + +2.2 608,181 - +2.2 608,191 - -0.6 608,191 - -0.1 608,191 - +1.5 608,191 - +0.8 608,193 + -0.9 608,201 - +0.4 608,201 - -0.7 608,211 + +0.1 608,231 + BBR_RS12725 0.10 +0.2 608,239 - BBR_RS12725 0.13 +0.5 608,347 + BBR_RS12725 0.41 +1.0 608,355 - BBR_RS12725 0.43 +1.5 608,357 - BBR_RS12725 0.44 +0.1 608,357 - BBR_RS12725 0.44 +0.4 608,379 + BBR_RS12725 0.49 +0.1 608,382 - BBR_RS12725 0.50 -1.4 608,397 + BBR_RS12725 0.54 +0.8 608,405 - BBR_RS12725 0.56 +1.2 608,424 - BBR_RS12725 0.61 -1.0 608,502 - BBR_RS12725 0.82 -0.4 608,553 + +2.7 608,555 + -1.5 608,555 + -0.4 608,555 + -0.2 608,561 - +0.4 608,563 - -1.1 608,563 - -0.8 608,599 + +3.0 608,602 + +0.9 608,604 + -0.2 608,662 - -0.6 608,663 + -0.0 608,663 + -1.3 608,671 - +1.1 608,676 - -0.6 608,676 - -0.2 608,676 - +0.6 608,676 - -0.3 608,676 - +0.5 608,676 - -0.3 608,749 - BBR_RS20515 0.11 +0.7 608,767 + BBR_RS20515 0.12 +2.1 608,767 - BBR_RS20515 0.12 -0.4 608,804 - BBR_RS20515 0.16 +0.2 608,841 + BBR_RS20515 0.19 +2.6 608,841 + BBR_RS20515 0.19 -1.0 608,864 - BBR_RS20515 0.21 +0.1 608,864 - BBR_RS20515 0.21 -0.0 608,866 + BBR_RS20515 0.22 +0.2 608,935 + BBR_RS20515 0.28 +0.6 608,942 + BBR_RS20515 0.29 +0.4 608,942 + BBR_RS20515 0.29 +0.5 608,950 - BBR_RS20515 0.30 -1.5 608,950 - BBR_RS20515 0.30 +0.8 608,960 - BBR_RS20515 0.31 +4.1 608,973 + BBR_RS20515 0.32 +0.9 608,973 + BBR_RS20515 0.32 -1.8 608,978 + BBR_RS20515 0.32 +0.5 608,981 - BBR_RS20515 0.32 -1.4 608,981 - BBR_RS20515 0.32 -0.4 608,981 - BBR_RS20515 0.32 -0.5 609,043 - BBR_RS20515 0.38 -0.3
Or see this region's nucleotide sequence