Experiment: Recovery broth with Ticarcillin 4e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12275 and BBR_RS12280 are separated by 627 nucleotides BBR_RS12280 and BBR_RS12285 are separated by 265 nucleotides
BBR_RS12275: BBR_RS12275 - DUF2207 domain-containing protein, at 504,710 to 506,185
_RS12275
BBR_RS12280: BBR_RS12280 - aldo/keto reductase, at 506,813 to 507,676
_RS12280
BBR_RS12285: BBR_RS12285 - nucleoside hydrolase, at 507,942 to 508,997
_RS12285
Position (kb) 506
507
508 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 505.847 kb on - strand, within BBR_RS12275 at 505.853 kb on + strand, within BBR_RS12275 at 505.853 kb on + strand, within BBR_RS12275 at 505.861 kb on + strand, within BBR_RS12275 at 505.861 kb on - strand, within BBR_RS12275 at 505.861 kb on - strand, within BBR_RS12275 at 505.869 kb on - strand, within BBR_RS12275 at 505.869 kb on - strand, within BBR_RS12275 at 505.877 kb on - strand, within BBR_RS12275 at 505.877 kb on - strand, within BBR_RS12275 at 505.940 kb on - strand, within BBR_RS12275 at 505.990 kb on - strand, within BBR_RS12275 at 505.997 kb on - strand, within BBR_RS12275 at 506.003 kb on + strand, within BBR_RS12275 at 506.004 kb on + strand, within BBR_RS12275 at 506.007 kb on - strand, within BBR_RS12275 at 506.025 kb on + strand, within BBR_RS12275 at 506.054 kb on + strand at 506.062 kb on - strand at 506.131 kb on - strand at 506.173 kb on - strand at 506.188 kb on - strand at 506.201 kb on - strand at 506.212 kb on + strand at 506.219 kb on + strand at 506.241 kb on + strand at 506.243 kb on - strand at 506.260 kb on - strand at 506.265 kb on + strand at 506.294 kb on + strand at 506.314 kb on - strand at 506.314 kb on - strand at 506.314 kb on - strand at 506.337 kb on - strand at 506.352 kb on + strand at 506.360 kb on - strand at 506.360 kb on - strand at 506.379 kb on + strand at 506.379 kb on + strand at 506.379 kb on + strand at 506.379 kb on + strand at 506.385 kb on - strand at 506.386 kb on + strand at 506.387 kb on - strand at 506.387 kb on - strand at 506.389 kb on + strand at 506.394 kb on + strand at 506.397 kb on - strand at 506.397 kb on - strand at 506.399 kb on + strand at 506.399 kb on + strand at 506.399 kb on + strand at 506.407 kb on - strand at 506.437 kb on + strand at 506.451 kb on + strand at 506.459 kb on - strand at 506.462 kb on + strand at 506.462 kb on + strand at 506.464 kb on + strand at 506.482 kb on - strand at 506.482 kb on - strand at 506.503 kb on + strand at 506.534 kb on + strand at 506.537 kb on + strand at 506.542 kb on + strand at 506.543 kb on + strand at 506.543 kb on + strand at 506.550 kb on - strand at 506.554 kb on + strand at 506.554 kb on + strand at 506.566 kb on + strand at 506.587 kb on - strand at 506.589 kb on + strand at 506.589 kb on + strand at 506.589 kb on + strand at 506.589 kb on + strand at 506.597 kb on - strand at 506.610 kb on - strand at 506.610 kb on - strand at 506.623 kb on - strand at 506.630 kb on - strand at 506.641 kb on - strand at 506.682 kb on - strand at 506.698 kb on - strand at 506.699 kb on - strand at 506.709 kb on + strand at 506.717 kb on - strand at 506.754 kb on + strand at 506.768 kb on + strand at 506.926 kb on - strand, within BBR_RS12280 at 506.969 kb on + strand, within BBR_RS12280 at 506.969 kb on + strand, within BBR_RS12280 at 506.969 kb on + strand, within BBR_RS12280 at 506.977 kb on - strand, within BBR_RS12280 at 506.977 kb on - strand, within BBR_RS12280 at 507.011 kb on - strand, within BBR_RS12280 at 507.011 kb on - strand, within BBR_RS12280 at 507.034 kb on + strand, within BBR_RS12280 at 507.049 kb on - strand, within BBR_RS12280 at 507.050 kb on + strand, within BBR_RS12280 at 507.064 kb on - strand, within BBR_RS12280 at 507.067 kb on + strand, within BBR_RS12280 at 507.074 kb on - strand, within BBR_RS12280 at 507.108 kb on - strand, within BBR_RS12280 at 507.115 kb on + strand, within BBR_RS12280 at 507.129 kb on - strand, within BBR_RS12280 at 507.152 kb on + strand, within BBR_RS12280 at 507.160 kb on - strand, within BBR_RS12280 at 507.162 kb on + strand, within BBR_RS12280 at 507.167 kb on + strand, within BBR_RS12280 at 507.170 kb on - strand, within BBR_RS12280 at 507.173 kb on - strand, within BBR_RS12280 at 507.179 kb on + strand, within BBR_RS12280 at 507.179 kb on + strand, within BBR_RS12280 at 507.194 kb on - strand, within BBR_RS12280 at 507.196 kb on + strand, within BBR_RS12280 at 507.210 kb on - strand, within BBR_RS12280 at 507.224 kb on + strand, within BBR_RS12280 at 507.224 kb on + strand, within BBR_RS12280 at 507.272 kb on + strand, within BBR_RS12280 at 507.280 kb on - strand, within BBR_RS12280 at 507.280 kb on - strand, within BBR_RS12280 at 507.280 kb on - strand, within BBR_RS12280 at 507.290 kb on + strand, within BBR_RS12280 at 507.290 kb on + strand, within BBR_RS12280 at 507.301 kb on + strand, within BBR_RS12280 at 507.301 kb on + strand, within BBR_RS12280 at 507.303 kb on - strand, within BBR_RS12280 at 507.314 kb on - strand, within BBR_RS12280 at 507.316 kb on + strand, within BBR_RS12280 at 507.316 kb on + strand, within BBR_RS12280 at 507.342 kb on + strand, within BBR_RS12280 at 507.342 kb on + strand, within BBR_RS12280 at 507.349 kb on + strand, within BBR_RS12280 at 507.349 kb on + strand, within BBR_RS12280 at 507.357 kb on - strand, within BBR_RS12280 at 507.357 kb on - strand, within BBR_RS12280 at 507.367 kb on - strand, within BBR_RS12280 at 507.370 kb on + strand, within BBR_RS12280 at 507.370 kb on + strand, within BBR_RS12280 at 507.378 kb on - strand, within BBR_RS12280 at 507.383 kb on - strand, within BBR_RS12280 at 507.385 kb on + strand, within BBR_RS12280 at 507.391 kb on - strand, within BBR_RS12280 at 507.392 kb on + strand, within BBR_RS12280 at 507.393 kb on + strand, within BBR_RS12280 at 507.393 kb on + strand, within BBR_RS12280 at 507.399 kb on - strand, within BBR_RS12280 at 507.404 kb on + strand, within BBR_RS12280 at 507.422 kb on - strand, within BBR_RS12280 at 507.422 kb on - strand, within BBR_RS12280 at 507.462 kb on + strand, within BBR_RS12280 at 507.470 kb on - strand, within BBR_RS12280 at 507.472 kb on - strand, within BBR_RS12280 at 507.481 kb on + strand, within BBR_RS12280 at 507.481 kb on + strand, within BBR_RS12280 at 507.530 kb on - strand, within BBR_RS12280 at 507.530 kb on - strand, within BBR_RS12280 at 507.611 kb on + strand at 507.619 kb on - strand at 507.621 kb on + strand at 507.622 kb on + strand at 507.622 kb on + strand at 507.622 kb on + strand at 507.630 kb on - strand at 507.630 kb on - strand at 507.635 kb on - strand at 507.648 kb on + strand at 507.656 kb on - strand at 507.656 kb on - strand at 507.675 kb on - strand at 507.934 kb on + strand at 507.937 kb on - strand at 507.942 kb on - strand at 508.005 kb on + strand at 508.008 kb on - strand at 508.018 kb on - strand at 508.018 kb on - strand at 508.018 kb on - strand at 508.070 kb on + strand, within BBR_RS12285 at 508.070 kb on - strand, within BBR_RS12285 at 508.108 kb on - strand, within BBR_RS12285 at 508.130 kb on + strand, within BBR_RS12285 at 508.149 kb on - strand, within BBR_RS12285 at 508.204 kb on - strand, within BBR_RS12285 at 508.209 kb on + strand, within BBR_RS12285 at 508.261 kb on - strand, within BBR_RS12285 at 508.263 kb on - strand, within BBR_RS12285 at 508.280 kb on + strand, within BBR_RS12285 at 508.290 kb on + strand, within BBR_RS12285 at 508.290 kb on + strand, within BBR_RS12285 at 508.291 kb on + strand, within BBR_RS12285 at 508.291 kb on + strand, within BBR_RS12285 at 508.298 kb on - strand, within BBR_RS12285 at 508.299 kb on - strand, within BBR_RS12285 at 508.316 kb on - strand, within BBR_RS12285 at 508.336 kb on + strand, within BBR_RS12285 at 508.336 kb on + strand, within BBR_RS12285 at 508.336 kb on + strand, within BBR_RS12285 at 508.344 kb on - strand, within BBR_RS12285 at 508.346 kb on + strand, within BBR_RS12285 at 508.358 kb on + strand, within BBR_RS12285 at 508.361 kb on - strand, within BBR_RS12285 at 508.407 kb on + strand, within BBR_RS12285 at 508.415 kb on - strand, within BBR_RS12285 at 508.415 kb on - strand, within BBR_RS12285 at 508.440 kb on + strand, within BBR_RS12285 at 508.448 kb on - strand, within BBR_RS12285 at 508.487 kb on + strand, within BBR_RS12285 at 508.531 kb on + strand, within BBR_RS12285 at 508.532 kb on + strand, within BBR_RS12285 at 508.558 kb on + strand, within BBR_RS12285 at 508.566 kb on - strand, within BBR_RS12285 at 508.589 kb on + strand, within BBR_RS12285 at 508.594 kb on + strand, within BBR_RS12285 at 508.594 kb on + strand, within BBR_RS12285 at 508.621 kb on + strand, within BBR_RS12285 at 508.635 kb on + strand, within BBR_RS12285
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 4e-04 mM remove 505,847 - BBR_RS12275 0.77 +0.2 505,853 + BBR_RS12275 0.77 -1.4 505,853 + BBR_RS12275 0.77 -0.7 505,861 + BBR_RS12275 0.78 +0.7 505,861 - BBR_RS12275 0.78 -0.9 505,861 - BBR_RS12275 0.78 -2.0 505,869 - BBR_RS12275 0.79 -0.5 505,869 - BBR_RS12275 0.79 -0.3 505,877 - BBR_RS12275 0.79 +1.0 505,877 - BBR_RS12275 0.79 +0.4 505,940 - BBR_RS12275 0.83 -0.8 505,990 - BBR_RS12275 0.87 -1.7 505,997 - BBR_RS12275 0.87 -0.8 506,003 + BBR_RS12275 0.88 -0.5 506,004 + BBR_RS12275 0.88 +2.1 506,007 - BBR_RS12275 0.88 -0.6 506,025 + BBR_RS12275 0.89 +1.8 506,054 + -0.8 506,062 - +1.3 506,131 - -1.1 506,173 - -0.1 506,188 - -0.1 506,201 - +1.3 506,212 + -0.0 506,219 + +1.8 506,241 + -1.3 506,243 - -0.7 506,260 - -0.2 506,265 + -2.5 506,294 + +3.7 506,314 - +0.5 506,314 - -1.7 506,314 - +0.8 506,337 - +0.6 506,352 + +4.8 506,360 - +0.5 506,360 - -0.6 506,379 + -0.5 506,379 + -0.8 506,379 + -0.7 506,379 + -1.8 506,385 - +0.6 506,386 + +0.1 506,387 - +0.5 506,387 - -2.1 506,389 + +4.5 506,394 + -0.1 506,397 - -0.9 506,397 - -1.0 506,399 + +0.5 506,399 + -0.1 506,399 + +0.3 506,407 - -1.2 506,437 + -0.2 506,451 + -0.7 506,459 - -1.4 506,462 + -2.0 506,462 + -0.5 506,464 + +2.6 506,482 - +1.1 506,482 - +0.7 506,503 + -1.0 506,534 + -1.0 506,537 + +0.2 506,542 + +0.2 506,543 + -0.3 506,543 + -0.8 506,550 - -0.2 506,554 + -1.8 506,554 + -0.8 506,566 + +0.6 506,587 - -0.4 506,589 + +0.4 506,589 + +0.6 506,589 + -2.2 506,589 + -0.2 506,597 - -0.8 506,610 - -1.4 506,610 - -0.2 506,623 - -0.1 506,630 - +1.7 506,641 - -0.7 506,682 - +0.2 506,698 - -0.2 506,699 - +1.6 506,709 + -1.5 506,717 - +0.3 506,754 + -1.3 506,768 + +0.5 506,926 - BBR_RS12280 0.13 +0.5 506,969 + BBR_RS12280 0.18 +0.8 506,969 + BBR_RS12280 0.18 -2.5 506,969 + BBR_RS12280 0.18 +1.2 506,977 - BBR_RS12280 0.19 +1.7 506,977 - BBR_RS12280 0.19 +4.5 507,011 - BBR_RS12280 0.23 +0.8 507,011 - BBR_RS12280 0.23 +0.1 507,034 + BBR_RS12280 0.26 +0.3 507,049 - BBR_RS12280 0.27 +0.2 507,050 + BBR_RS12280 0.27 +0.1 507,064 - BBR_RS12280 0.29 +0.9 507,067 + BBR_RS12280 0.29 +0.3 507,074 - BBR_RS12280 0.30 +0.7 507,108 - BBR_RS12280 0.34 +2.8 507,115 + BBR_RS12280 0.35 -0.5 507,129 - BBR_RS12280 0.37 +0.3 507,152 + BBR_RS12280 0.39 +2.4 507,160 - BBR_RS12280 0.40 +1.6 507,162 + BBR_RS12280 0.40 +0.4 507,167 + BBR_RS12280 0.41 -0.6 507,170 - BBR_RS12280 0.41 +0.5 507,173 - BBR_RS12280 0.42 +0.0 507,179 + BBR_RS12280 0.42 -0.0 507,179 + BBR_RS12280 0.42 +0.5 507,194 - BBR_RS12280 0.44 +1.5 507,196 + BBR_RS12280 0.44 +0.8 507,210 - BBR_RS12280 0.46 -0.6 507,224 + BBR_RS12280 0.48 +0.1 507,224 + BBR_RS12280 0.48 -0.2 507,272 + BBR_RS12280 0.53 +1.5 507,280 - BBR_RS12280 0.54 +2.0 507,280 - BBR_RS12280 0.54 -0.5 507,280 - BBR_RS12280 0.54 +0.7 507,290 + BBR_RS12280 0.55 +0.1 507,290 + BBR_RS12280 0.55 +0.4 507,301 + BBR_RS12280 0.56 +0.2 507,301 + BBR_RS12280 0.56 +0.6 507,303 - BBR_RS12280 0.57 +0.6 507,314 - BBR_RS12280 0.58 +0.7 507,316 + BBR_RS12280 0.58 -1.2 507,316 + BBR_RS12280 0.58 +0.4 507,342 + BBR_RS12280 0.61 -1.8 507,342 + BBR_RS12280 0.61 +1.6 507,349 + BBR_RS12280 0.62 +1.7 507,349 + BBR_RS12280 0.62 +1.7 507,357 - BBR_RS12280 0.63 +2.2 507,357 - BBR_RS12280 0.63 +0.9 507,367 - BBR_RS12280 0.64 +2.0 507,370 + BBR_RS12280 0.64 -0.0 507,370 + BBR_RS12280 0.64 +0.4 507,378 - BBR_RS12280 0.65 +1.8 507,383 - BBR_RS12280 0.66 +1.5 507,385 + BBR_RS12280 0.66 +0.0 507,391 - BBR_RS12280 0.67 -1.5 507,392 + BBR_RS12280 0.67 +0.8 507,393 + BBR_RS12280 0.67 -0.2 507,393 + BBR_RS12280 0.67 +0.1 507,399 - BBR_RS12280 0.68 +0.1 507,404 + BBR_RS12280 0.68 +1.8 507,422 - BBR_RS12280 0.70 +0.2 507,422 - BBR_RS12280 0.70 +0.6 507,462 + BBR_RS12280 0.75 +1.5 507,470 - BBR_RS12280 0.76 -0.2 507,472 - BBR_RS12280 0.76 +0.5 507,481 + BBR_RS12280 0.77 +1.8 507,481 + BBR_RS12280 0.77 +0.2 507,530 - BBR_RS12280 0.83 +1.9 507,530 - BBR_RS12280 0.83 +0.4 507,611 + +0.9 507,619 - +1.0 507,621 + +0.9 507,622 + -0.2 507,622 + +1.3 507,622 + +0.2 507,630 - +0.1 507,630 - +0.1 507,635 - -0.7 507,648 + -2.1 507,656 - +2.1 507,656 - +1.8 507,675 - -0.3 507,934 + +0.8 507,937 - +0.5 507,942 - -1.0 508,005 + -1.1 508,008 - +1.1 508,018 - +1.5 508,018 - +1.1 508,018 - +0.5 508,070 + BBR_RS12285 0.12 +4.3 508,070 - BBR_RS12285 0.12 +0.4 508,108 - BBR_RS12285 0.16 +0.3 508,130 + BBR_RS12285 0.18 +0.8 508,149 - BBR_RS12285 0.20 +0.6 508,204 - BBR_RS12285 0.25 -0.9 508,209 + BBR_RS12285 0.25 +1.0 508,261 - BBR_RS12285 0.30 +1.1 508,263 - BBR_RS12285 0.30 -0.3 508,280 + BBR_RS12285 0.32 -1.5 508,290 + BBR_RS12285 0.33 +3.0 508,290 + BBR_RS12285 0.33 +0.7 508,291 + BBR_RS12285 0.33 +1.6 508,291 + BBR_RS12285 0.33 -1.2 508,298 - BBR_RS12285 0.34 -0.1 508,299 - BBR_RS12285 0.34 -0.7 508,316 - BBR_RS12285 0.35 -0.8 508,336 + BBR_RS12285 0.37 +2.5 508,336 + BBR_RS12285 0.37 -1.6 508,336 + BBR_RS12285 0.37 +1.5 508,344 - BBR_RS12285 0.38 -0.3 508,346 + BBR_RS12285 0.38 +0.1 508,358 + BBR_RS12285 0.39 -1.1 508,361 - BBR_RS12285 0.40 -1.1 508,407 + BBR_RS12285 0.44 -0.2 508,415 - BBR_RS12285 0.45 +2.0 508,415 - BBR_RS12285 0.45 +2.4 508,440 + BBR_RS12285 0.47 -0.1 508,448 - BBR_RS12285 0.48 -0.8 508,487 + BBR_RS12285 0.52 -0.8 508,531 + BBR_RS12285 0.56 -1.0 508,532 + BBR_RS12285 0.56 -1.4 508,558 + BBR_RS12285 0.58 -0.2 508,566 - BBR_RS12285 0.59 -0.7 508,589 + BBR_RS12285 0.61 -0.2 508,594 + BBR_RS12285 0.62 +0.5 508,594 + BBR_RS12285 0.62 +0.7 508,621 + BBR_RS12285 0.64 +0.1 508,635 + BBR_RS12285 0.66 -1.6
Or see this region's nucleotide sequence