Experiment: Recovery broth with Ticarcillin 4e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11630 and BBR_RS11635 are separated by 165 nucleotides BBR_RS11635 and BBR_RS11640 are separated by 15 nucleotides BBR_RS11640 and BBR_RS11645 are separated by 46 nucleotides BBR_RS11645 and BBR_RS11650 are separated by 33 nucleotides
BBR_RS11630: BBR_RS11630 - MFS transporter, at 365,435 to 366,640
_RS11630
BBR_RS11635: BBR_RS11635 - hypothetical protein, at 366,806 to 367,117
_RS11635
BBR_RS11640: BBR_RS11640 - hypothetical protein, at 367,133 to 367,498
_RS11640
BBR_RS11645: BBR_RS11645 - integrase, at 367,545 to 367,706
_RS11645
BBR_RS11650: BBR_RS11650 - integrase, at 367,740 to 368,417
_RS11650
Position (kb) 366
367
368 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 365.832 kb on - strand, within BBR_RS11630 at 365.842 kb on + strand, within BBR_RS11630 at 365.868 kb on + strand, within BBR_RS11630 at 365.871 kb on - strand, within BBR_RS11630 at 365.900 kb on + strand, within BBR_RS11630 at 365.908 kb on - strand, within BBR_RS11630 at 365.914 kb on + strand, within BBR_RS11630 at 365.920 kb on - strand, within BBR_RS11630 at 365.945 kb on + strand, within BBR_RS11630 at 365.950 kb on + strand, within BBR_RS11630 at 365.969 kb on + strand, within BBR_RS11630 at 365.993 kb on + strand, within BBR_RS11630 at 366.035 kb on - strand, within BBR_RS11630 at 366.035 kb on - strand, within BBR_RS11630 at 366.035 kb on - strand, within BBR_RS11630 at 366.040 kb on + strand, within BBR_RS11630 at 366.121 kb on - strand, within BBR_RS11630 at 366.121 kb on - strand, within BBR_RS11630 at 366.164 kb on + strand, within BBR_RS11630 at 366.164 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on + strand, within BBR_RS11630 at 366.170 kb on - strand, within BBR_RS11630 at 366.170 kb on - strand, within BBR_RS11630 at 366.172 kb on - strand, within BBR_RS11630 at 366.172 kb on - strand, within BBR_RS11630 at 366.172 kb on - strand, within BBR_RS11630 at 366.172 kb on - strand, within BBR_RS11630 at 366.178 kb on - strand, within BBR_RS11630 at 366.178 kb on - strand, within BBR_RS11630 at 366.202 kb on + strand, within BBR_RS11630 at 366.224 kb on - strand, within BBR_RS11630 at 366.241 kb on + strand, within BBR_RS11630 at 366.244 kb on + strand, within BBR_RS11630 at 366.246 kb on + strand, within BBR_RS11630 at 366.246 kb on + strand, within BBR_RS11630 at 366.254 kb on - strand, within BBR_RS11630 at 366.254 kb on - strand, within BBR_RS11630 at 366.301 kb on + strand, within BBR_RS11630 at 366.301 kb on + strand, within BBR_RS11630 at 366.309 kb on - strand, within BBR_RS11630 at 366.309 kb on - strand, within BBR_RS11630 at 366.309 kb on - strand, within BBR_RS11630 at 366.319 kb on - strand, within BBR_RS11630 at 366.319 kb on - strand, within BBR_RS11630 at 366.328 kb on + strand, within BBR_RS11630 at 366.328 kb on + strand, within BBR_RS11630 at 366.336 kb on - strand, within BBR_RS11630 at 366.369 kb on + strand, within BBR_RS11630 at 366.377 kb on - strand, within BBR_RS11630 at 366.396 kb on + strand, within BBR_RS11630 at 366.400 kb on + strand, within BBR_RS11630 at 366.403 kb on - strand, within BBR_RS11630 at 366.420 kb on - strand, within BBR_RS11630 at 366.420 kb on - strand, within BBR_RS11630 at 366.420 kb on - strand, within BBR_RS11630 at 366.420 kb on - strand, within BBR_RS11630 at 366.420 kb on - strand, within BBR_RS11630 at 366.425 kb on - strand, within BBR_RS11630 at 366.445 kb on + strand, within BBR_RS11630 at 366.453 kb on - strand, within BBR_RS11630 at 366.457 kb on + strand, within BBR_RS11630 at 366.465 kb on - strand, within BBR_RS11630 at 366.514 kb on + strand, within BBR_RS11630 at 366.514 kb on + strand, within BBR_RS11630 at 366.520 kb on - strand at 366.520 kb on - strand at 366.553 kb on + strand at 366.575 kb on + strand at 366.609 kb on - strand at 366.656 kb on + strand at 366.662 kb on - strand at 366.665 kb on + strand at 366.672 kb on - strand at 366.682 kb on - strand at 366.686 kb on + strand at 366.689 kb on + strand at 366.707 kb on - strand at 366.717 kb on + strand at 366.733 kb on + strand at 366.743 kb on - strand at 366.751 kb on + strand at 366.751 kb on + strand at 366.864 kb on + strand, within BBR_RS11635 at 366.872 kb on - strand, within BBR_RS11635 at 366.891 kb on - strand, within BBR_RS11635 at 366.897 kb on - strand, within BBR_RS11635 at 366.916 kb on - strand, within BBR_RS11635 at 366.919 kb on + strand, within BBR_RS11635 at 366.928 kb on + strand, within BBR_RS11635 at 366.942 kb on - strand, within BBR_RS11635 at 366.958 kb on + strand, within BBR_RS11635 at 366.963 kb on + strand, within BBR_RS11635 at 366.963 kb on + strand, within BBR_RS11635 at 366.965 kb on + strand, within BBR_RS11635 at 366.966 kb on - strand, within BBR_RS11635 at 366.990 kb on - strand, within BBR_RS11635 at 366.997 kb on - strand, within BBR_RS11635 at 367.009 kb on - strand, within BBR_RS11635 at 367.027 kb on + strand, within BBR_RS11635 at 367.035 kb on + strand, within BBR_RS11635 at 367.035 kb on + strand, within BBR_RS11635 at 367.043 kb on - strand, within BBR_RS11635 at 367.044 kb on + strand, within BBR_RS11635 at 367.056 kb on - strand, within BBR_RS11635 at 367.064 kb on + strand, within BBR_RS11635 at 367.296 kb on - strand, within BBR_RS11640 at 367.353 kb on + strand, within BBR_RS11640 at 367.431 kb on + strand, within BBR_RS11640 at 367.444 kb on + strand, within BBR_RS11640 at 367.476 kb on - strand at 367.493 kb on + strand at 367.515 kb on - strand at 367.515 kb on - strand at 367.546 kb on - strand at 367.546 kb on - strand at 367.556 kb on - strand at 367.556 kb on - strand at 367.569 kb on + strand, within BBR_RS11645 at 367.574 kb on + strand, within BBR_RS11645 at 367.574 kb on + strand, within BBR_RS11645 at 367.578 kb on + strand, within BBR_RS11645 at 367.582 kb on - strand, within BBR_RS11645 at 367.584 kb on - strand, within BBR_RS11645 at 367.601 kb on + strand, within BBR_RS11645 at 367.621 kb on - strand, within BBR_RS11645 at 367.628 kb on - strand, within BBR_RS11645 at 367.647 kb on + strand, within BBR_RS11645 at 367.654 kb on + strand, within BBR_RS11645 at 367.672 kb on + strand, within BBR_RS11645 at 367.695 kb on + strand at 367.702 kb on - strand at 367.704 kb on + strand at 367.716 kb on - strand at 367.755 kb on - strand at 367.770 kb on + strand at 367.773 kb on - strand at 367.778 kb on - strand at 367.780 kb on + strand at 367.788 kb on - strand at 367.800 kb on - strand at 367.836 kb on + strand, within BBR_RS11650 at 367.836 kb on + strand, within BBR_RS11650 at 367.836 kb on - strand, within BBR_RS11650 at 367.849 kb on + strand, within BBR_RS11650 at 367.888 kb on + strand, within BBR_RS11650 at 367.888 kb on + strand, within BBR_RS11650 at 367.896 kb on - strand, within BBR_RS11650 at 367.946 kb on + strand, within BBR_RS11650 at 367.946 kb on + strand, within BBR_RS11650 at 367.946 kb on + strand, within BBR_RS11650 at 367.980 kb on + strand, within BBR_RS11650 at 367.981 kb on - strand, within BBR_RS11650 at 367.986 kb on - strand, within BBR_RS11650 at 367.988 kb on - strand, within BBR_RS11650 at 368.011 kb on - strand, within BBR_RS11650 at 368.018 kb on - strand, within BBR_RS11650 at 368.020 kb on + strand, within BBR_RS11650 at 368.035 kb on + strand, within BBR_RS11650 at 368.046 kb on + strand, within BBR_RS11650 at 368.046 kb on + strand, within BBR_RS11650 at 368.061 kb on + strand, within BBR_RS11650 at 368.061 kb on + strand, within BBR_RS11650 at 368.061 kb on + strand, within BBR_RS11650 at 368.065 kb on + strand, within BBR_RS11650 at 368.086 kb on + strand, within BBR_RS11650
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ticarcillin 4e-04 mM remove 365,832 - BBR_RS11630 0.33 -1.5 365,842 + BBR_RS11630 0.34 +0.8 365,868 + BBR_RS11630 0.36 +0.3 365,871 - BBR_RS11630 0.36 -0.8 365,900 + BBR_RS11630 0.39 -2.3 365,908 - BBR_RS11630 0.39 -1.0 365,914 + BBR_RS11630 0.40 +0.0 365,920 - BBR_RS11630 0.40 +1.2 365,945 + BBR_RS11630 0.42 -0.1 365,950 + BBR_RS11630 0.43 -2.5 365,969 + BBR_RS11630 0.44 +0.1 365,993 + BBR_RS11630 0.46 -0.2 366,035 - BBR_RS11630 0.50 +0.1 366,035 - BBR_RS11630 0.50 +0.3 366,035 - BBR_RS11630 0.50 +0.8 366,040 + BBR_RS11630 0.50 +0.6 366,121 - BBR_RS11630 0.57 +2.0 366,121 - BBR_RS11630 0.57 -0.6 366,164 + BBR_RS11630 0.60 -0.8 366,164 + BBR_RS11630 0.60 +1.9 366,170 + BBR_RS11630 0.61 -0.1 366,170 + BBR_RS11630 0.61 +0.4 366,170 + BBR_RS11630 0.61 -1.2 366,170 + BBR_RS11630 0.61 -0.4 366,170 + BBR_RS11630 0.61 +0.6 366,170 + BBR_RS11630 0.61 -0.8 366,170 + BBR_RS11630 0.61 +0.2 366,170 - BBR_RS11630 0.61 +1.6 366,170 - BBR_RS11630 0.61 -1.1 366,172 - BBR_RS11630 0.61 +0.6 366,172 - BBR_RS11630 0.61 -0.5 366,172 - BBR_RS11630 0.61 -0.1 366,172 - BBR_RS11630 0.61 -0.4 366,178 - BBR_RS11630 0.62 -0.9 366,178 - BBR_RS11630 0.62 +1.4 366,202 + BBR_RS11630 0.64 +0.0 366,224 - BBR_RS11630 0.65 -1.0 366,241 + BBR_RS11630 0.67 +1.0 366,244 + BBR_RS11630 0.67 -0.7 366,246 + BBR_RS11630 0.67 +0.2 366,246 + BBR_RS11630 0.67 +1.2 366,254 - BBR_RS11630 0.68 +0.0 366,254 - BBR_RS11630 0.68 -0.8 366,301 + BBR_RS11630 0.72 -1.5 366,301 + BBR_RS11630 0.72 -0.2 366,309 - BBR_RS11630 0.72 +0.0 366,309 - BBR_RS11630 0.72 -0.7 366,309 - BBR_RS11630 0.72 -1.2 366,319 - BBR_RS11630 0.73 -0.0 366,319 - BBR_RS11630 0.73 +0.9 366,328 + BBR_RS11630 0.74 -0.4 366,328 + BBR_RS11630 0.74 -0.9 366,336 - BBR_RS11630 0.75 +1.1 366,369 + BBR_RS11630 0.77 -1.0 366,377 - BBR_RS11630 0.78 +0.5 366,396 + BBR_RS11630 0.80 -0.3 366,400 + BBR_RS11630 0.80 +0.8 366,403 - BBR_RS11630 0.80 +3.2 366,420 - BBR_RS11630 0.82 -2.1 366,420 - BBR_RS11630 0.82 +1.5 366,420 - BBR_RS11630 0.82 -2.6 366,420 - BBR_RS11630 0.82 +0.2 366,420 - BBR_RS11630 0.82 -0.7 366,425 - BBR_RS11630 0.82 -0.2 366,445 + BBR_RS11630 0.84 +1.4 366,453 - BBR_RS11630 0.84 -0.1 366,457 + BBR_RS11630 0.85 -0.2 366,465 - BBR_RS11630 0.85 +1.1 366,514 + BBR_RS11630 0.89 +0.1 366,514 + BBR_RS11630 0.89 +0.0 366,520 - +2.1 366,520 - +0.5 366,553 + +0.6 366,575 + +1.0 366,609 - -0.4 366,656 + +0.5 366,662 - -1.1 366,665 + +1.0 366,672 - +1.3 366,682 - -0.4 366,686 + +0.1 366,689 + -1.6 366,707 - +1.0 366,717 + +0.5 366,733 + +0.2 366,743 - -1.1 366,751 + -0.8 366,751 + -0.2 366,864 + BBR_RS11635 0.19 -1.3 366,872 - BBR_RS11635 0.21 +2.8 366,891 - BBR_RS11635 0.27 -2.1 366,897 - BBR_RS11635 0.29 +0.2 366,916 - BBR_RS11635 0.35 -1.3 366,919 + BBR_RS11635 0.36 -0.9 366,928 + BBR_RS11635 0.39 +0.5 366,942 - BBR_RS11635 0.44 +0.0 366,958 + BBR_RS11635 0.49 -0.8 366,963 + BBR_RS11635 0.50 -0.2 366,963 + BBR_RS11635 0.50 -0.5 366,965 + BBR_RS11635 0.51 -1.2 366,966 - BBR_RS11635 0.51 +0.0 366,990 - BBR_RS11635 0.59 +0.8 366,997 - BBR_RS11635 0.61 +0.7 367,009 - BBR_RS11635 0.65 -0.9 367,027 + BBR_RS11635 0.71 -3.0 367,035 + BBR_RS11635 0.73 -1.0 367,035 + BBR_RS11635 0.73 +0.0 367,043 - BBR_RS11635 0.76 -0.3 367,044 + BBR_RS11635 0.76 -0.4 367,056 - BBR_RS11635 0.80 +0.3 367,064 + BBR_RS11635 0.83 -1.5 367,296 - BBR_RS11640 0.45 +1.9 367,353 + BBR_RS11640 0.60 -0.3 367,431 + BBR_RS11640 0.81 -1.6 367,444 + BBR_RS11640 0.85 +3.8 367,476 - -2.0 367,493 + -0.0 367,515 - -0.3 367,515 - +6.0 367,546 - +1.1 367,546 - -1.7 367,556 - -0.3 367,556 - -1.6 367,569 + BBR_RS11645 0.15 -0.4 367,574 + BBR_RS11645 0.18 -0.9 367,574 + BBR_RS11645 0.18 +0.2 367,578 + BBR_RS11645 0.20 +0.3 367,582 - BBR_RS11645 0.23 +0.3 367,584 - BBR_RS11645 0.24 -0.2 367,601 + BBR_RS11645 0.35 +1.7 367,621 - BBR_RS11645 0.47 +0.1 367,628 - BBR_RS11645 0.51 -0.9 367,647 + BBR_RS11645 0.63 +3.1 367,654 + BBR_RS11645 0.67 -0.2 367,672 + BBR_RS11645 0.78 -0.4 367,695 + +2.8 367,702 - +0.1 367,704 + -0.6 367,716 - +0.3 367,755 - +0.3 367,770 + +1.4 367,773 - -0.5 367,778 - -0.5 367,780 + +0.5 367,788 - +2.0 367,800 - -0.6 367,836 + BBR_RS11650 0.14 +0.4 367,836 + BBR_RS11650 0.14 +2.4 367,836 - BBR_RS11650 0.14 +1.5 367,849 + BBR_RS11650 0.16 +0.8 367,888 + BBR_RS11650 0.22 +0.4 367,888 + BBR_RS11650 0.22 +0.4 367,896 - BBR_RS11650 0.23 +0.2 367,946 + BBR_RS11650 0.30 -1.1 367,946 + BBR_RS11650 0.30 -0.5 367,946 + BBR_RS11650 0.30 -1.3 367,980 + BBR_RS11650 0.35 +0.3 367,981 - BBR_RS11650 0.36 +1.1 367,986 - BBR_RS11650 0.36 +0.8 367,988 - BBR_RS11650 0.37 -0.2 368,011 - BBR_RS11650 0.40 -0.3 368,018 - BBR_RS11650 0.41 +0.0 368,020 + BBR_RS11650 0.41 +0.1 368,035 + BBR_RS11650 0.44 +1.8 368,046 + BBR_RS11650 0.45 -0.8 368,046 + BBR_RS11650 0.45 +0.2 368,061 + BBR_RS11650 0.47 -1.2 368,061 + BBR_RS11650 0.47 -0.5 368,061 + BBR_RS11650 0.47 -1.1 368,065 + BBR_RS11650 0.48 +0.9 368,086 + BBR_RS11650 0.51 +2.2
Or see this region's nucleotide sequence