Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_847 and Pf1N1B4_848 are separated by 164 nucleotides Pf1N1B4_848 and Pf1N1B4_849 overlap by 13 nucleotides Pf1N1B4_849 and Pf1N1B4_850 are separated by 151 nucleotides
Pf1N1B4_847 - 2-isopropylmalate synthase (EC 2.3.3.13), at 974,985 to 976,664
_847
Pf1N1B4_848 - Aliphatic amidase AmiE (EC 3.5.1.4), at 976,829 to 977,620
_848
Pf1N1B4_849 - Aspartate aminotransferase (EC 2.6.1.1), at 977,608 to 978,756
_849
Pf1N1B4_850 - GTP-binding protein EngA, at 978,908 to 980,377
_850
Position (kb) 977
978
979 Strain fitness (log2 ratio) -2
-1
0
1
2 at 976.988 kb on - strand, within Pf1N1B4_848 at 976.989 kb on + strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 976.990 kb on - strand, within Pf1N1B4_848 at 977.037 kb on + strand, within Pf1N1B4_848 at 977.037 kb on + strand at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.038 kb on - strand, within Pf1N1B4_848 at 977.116 kb on - strand, within Pf1N1B4_848 at 977.173 kb on - strand, within Pf1N1B4_848 at 977.283 kb on + strand, within Pf1N1B4_848 at 977.284 kb on - strand, within Pf1N1B4_848 at 977.410 kb on + strand, within Pf1N1B4_848 at 977.410 kb on + strand, within Pf1N1B4_848 at 977.411 kb on - strand, within Pf1N1B4_848 at 977.620 kb on + strand at 977.621 kb on - strand at 977.621 kb on - strand at 977.621 kb on - strand at 977.705 kb on + strand at 977.705 kb on + strand at 977.705 kb on + strand at 977.705 kb on + strand at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.816 kb on + strand, within Pf1N1B4_849 at 977.817 kb on - strand, within Pf1N1B4_849 at 977.817 kb on - strand, within Pf1N1B4_849 at 977.817 kb on - strand, within Pf1N1B4_849 at 977.821 kb on - strand, within Pf1N1B4_849 at 977.891 kb on + strand, within Pf1N1B4_849 at 977.891 kb on + strand, within Pf1N1B4_849 at 977.891 kb on + strand, within Pf1N1B4_849 at 977.892 kb on - strand, within Pf1N1B4_849 at 977.892 kb on - strand, within Pf1N1B4_849 at 977.892 kb on - strand, within Pf1N1B4_849 at 977.936 kb on + strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.937 kb on - strand, within Pf1N1B4_849 at 977.951 kb on + strand, within Pf1N1B4_849 at 977.952 kb on - strand, within Pf1N1B4_849 at 977.952 kb on - strand, within Pf1N1B4_849 at 977.984 kb on + strand, within Pf1N1B4_849 at 977.984 kb on + strand, within Pf1N1B4_849 at 977.985 kb on - strand, within Pf1N1B4_849 at 977.985 kb on - strand, within Pf1N1B4_849 at 978.036 kb on - strand, within Pf1N1B4_849 at 978.062 kb on + strand, within Pf1N1B4_849 at 978.062 kb on + strand, within Pf1N1B4_849 at 978.063 kb on - strand, within Pf1N1B4_849 at 978.063 kb on - strand, within Pf1N1B4_849 at 978.122 kb on - strand, within Pf1N1B4_849 at 978.137 kb on - strand, within Pf1N1B4_849 at 978.137 kb on - strand, within Pf1N1B4_849 at 978.137 kb on - strand, within Pf1N1B4_849 at 978.148 kb on + strand, within Pf1N1B4_849 at 978.162 kb on - strand at 978.182 kb on + strand at 978.182 kb on + strand at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.182 kb on + strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.183 kb on - strand, within Pf1N1B4_849 at 978.187 kb on - strand, within Pf1N1B4_849 at 978.267 kb on + strand, within Pf1N1B4_849 at 978.268 kb on - strand at 978.392 kb on + strand, within Pf1N1B4_849 at 978.392 kb on + strand, within Pf1N1B4_849 at 978.392 kb on + strand, within Pf1N1B4_849 at 978.392 kb on + strand, within Pf1N1B4_849 at 978.393 kb on - strand, within Pf1N1B4_849 at 978.393 kb on - strand, within Pf1N1B4_849 at 978.393 kb on - strand, within Pf1N1B4_849 at 978.396 kb on + strand, within Pf1N1B4_849 at 978.397 kb on - strand, within Pf1N1B4_849 at 978.397 kb on - strand, within Pf1N1B4_849 at 978.402 kb on - strand, within Pf1N1B4_849 at 978.461 kb on - strand at 978.720 kb on + strand at 978.720 kb on + strand at 978.721 kb on - strand at 978.744 kb on + strand at 978.745 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 976,988 - Pf1N1B4_848 0.20 -0.3 976,989 + Pf1N1B4_848 0.20 +1.1 976,990 - Pf1N1B4_848 0.20 +0.1 976,990 - Pf1N1B4_848 0.20 -0.2 976,990 - Pf1N1B4_848 0.20 -0.5 976,990 - Pf1N1B4_848 0.20 +0.1 977,037 + Pf1N1B4_848 0.26 +0.3 977,037 + -0.5 977,038 - Pf1N1B4_848 0.26 +1.4 977,038 - Pf1N1B4_848 0.26 -0.5 977,038 - Pf1N1B4_848 0.26 -1.2 977,038 - Pf1N1B4_848 0.26 +0.3 977,038 - Pf1N1B4_848 0.26 -0.9 977,116 - Pf1N1B4_848 0.36 -1.0 977,173 - Pf1N1B4_848 0.43 +0.1 977,283 + Pf1N1B4_848 0.57 +0.8 977,284 - Pf1N1B4_848 0.57 +1.1 977,410 + Pf1N1B4_848 0.73 +1.7 977,410 + Pf1N1B4_848 0.73 -1.2 977,411 - Pf1N1B4_848 0.73 -1.7 977,620 + +0.4 977,621 - -0.0 977,621 - +0.6 977,621 - -0.9 977,705 + -2.1 977,705 + -1.2 977,705 + -1.7 977,705 + -0.5 977,816 + Pf1N1B4_849 0.18 -1.7 977,816 + Pf1N1B4_849 0.18 -0.7 977,816 + +1.1 977,816 + Pf1N1B4_849 0.18 -0.9 977,816 + Pf1N1B4_849 0.18 -0.8 977,816 + Pf1N1B4_849 0.18 -1.1 977,816 + Pf1N1B4_849 0.18 +1.5 977,816 + Pf1N1B4_849 0.18 -0.1 977,816 + Pf1N1B4_849 0.18 +0.5 977,816 + Pf1N1B4_849 0.18 +0.1 977,817 - Pf1N1B4_849 0.18 -0.7 977,817 - Pf1N1B4_849 0.18 -0.5 977,817 - Pf1N1B4_849 0.18 +0.1 977,821 - Pf1N1B4_849 0.19 -0.6 977,891 + Pf1N1B4_849 0.25 -0.9 977,891 + Pf1N1B4_849 0.25 +0.1 977,891 + Pf1N1B4_849 0.25 -0.5 977,892 - Pf1N1B4_849 0.25 -1.5 977,892 - Pf1N1B4_849 0.25 +1.9 977,892 - Pf1N1B4_849 0.25 -1.2 977,936 + Pf1N1B4_849 0.29 +0.7 977,937 - Pf1N1B4_849 0.29 -0.7 977,937 - Pf1N1B4_849 0.29 -1.7 977,937 - Pf1N1B4_849 0.29 -0.4 977,937 - Pf1N1B4_849 0.29 +0.4 977,951 + Pf1N1B4_849 0.30 +0.5 977,952 - Pf1N1B4_849 0.30 -1.5 977,952 - Pf1N1B4_849 0.30 -1.2 977,984 + Pf1N1B4_849 0.33 +0.4 977,984 + Pf1N1B4_849 0.33 -0.2 977,985 - Pf1N1B4_849 0.33 -2.4 977,985 - Pf1N1B4_849 0.33 -1.5 978,036 - Pf1N1B4_849 0.37 -0.9 978,062 + Pf1N1B4_849 0.40 +2.5 978,062 + Pf1N1B4_849 0.40 +0.3 978,063 - Pf1N1B4_849 0.40 +0.1 978,063 - Pf1N1B4_849 0.40 +1.7 978,122 - Pf1N1B4_849 0.45 -0.1 978,137 - Pf1N1B4_849 0.46 +0.1 978,137 - Pf1N1B4_849 0.46 +0.1 978,137 - Pf1N1B4_849 0.46 +1.1 978,148 + Pf1N1B4_849 0.47 +0.5 978,162 - -0.5 978,182 + +0.7 978,182 + -1.2 978,182 + Pf1N1B4_849 0.50 +0.8 978,182 + Pf1N1B4_849 0.50 +0.5 978,182 + Pf1N1B4_849 0.50 +1.0 978,182 + Pf1N1B4_849 0.50 -0.3 978,182 + Pf1N1B4_849 0.50 -0.5 978,182 + Pf1N1B4_849 0.50 -1.0 978,182 + Pf1N1B4_849 0.50 +0.5 978,182 + Pf1N1B4_849 0.50 +0.3 978,183 - Pf1N1B4_849 0.50 -0.5 978,183 - Pf1N1B4_849 0.50 -0.3 978,183 - Pf1N1B4_849 0.50 +2.1 978,183 - Pf1N1B4_849 0.50 +0.2 978,183 - Pf1N1B4_849 0.50 +0.1 978,183 - Pf1N1B4_849 0.50 -1.2 978,183 - Pf1N1B4_849 0.50 -0.9 978,183 - Pf1N1B4_849 0.50 +0.1 978,183 - Pf1N1B4_849 0.50 +0.1 978,183 - Pf1N1B4_849 0.50 -0.5 978,183 - Pf1N1B4_849 0.50 -2.5 978,187 - Pf1N1B4_849 0.50 -1.0 978,267 + Pf1N1B4_849 0.57 +0.8 978,268 - -0.9 978,392 + Pf1N1B4_849 0.68 +1.1 978,392 + Pf1N1B4_849 0.68 +1.5 978,392 + Pf1N1B4_849 0.68 +0.6 978,392 + Pf1N1B4_849 0.68 +0.3 978,393 - Pf1N1B4_849 0.68 +0.1 978,393 - Pf1N1B4_849 0.68 -1.7 978,393 - Pf1N1B4_849 0.68 +0.1 978,396 + Pf1N1B4_849 0.69 +0.4 978,397 - Pf1N1B4_849 0.69 +0.7 978,397 - Pf1N1B4_849 0.69 -1.2 978,402 - Pf1N1B4_849 0.69 +1.9 978,461 - +1.7 978,720 + -0.4 978,720 + -1.2 978,721 - -0.9 978,744 + -0.7 978,745 - +1.8
Or see this region's nucleotide sequence