Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_843 and Pf1N1B4_844 are separated by 59 nucleotides Pf1N1B4_844 and Pf1N1B4_845 are separated by 3 nucleotides
Pf1N1B4_843 - Transcriptional regulator, LysR family, at 971,292 to 972,242
_843
Pf1N1B4_844 - Exodeoxyribonuclease VII large subunit (EC 3.1.11.6), at 972,302 to 973,708
_844
Pf1N1B4_845 - Membrane proteins related to metalloendopeptidases, at 973,712 to 974,533
_845
Position (kb)
972
973
974 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 971.367 kb on + strand at 971.372 kb on + strand at 971.540 kb on + strand, within Pf1N1B4_843 at 971.541 kb on - strand, within Pf1N1B4_843 at 971.600 kb on + strand, within Pf1N1B4_843 at 971.600 kb on + strand, within Pf1N1B4_843 at 971.601 kb on - strand, within Pf1N1B4_843 at 971.601 kb on - strand, within Pf1N1B4_843 at 971.634 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.784 kb on + strand, within Pf1N1B4_843 at 971.816 kb on + strand, within Pf1N1B4_843 at 971.816 kb on + strand at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.922 kb on + strand, within Pf1N1B4_843 at 971.923 kb on - strand, within Pf1N1B4_843 at 972.161 kb on + strand at 972.161 kb on + strand at 972.161 kb on + strand at 972.161 kb on + strand at 972.162 kb on - strand at 972.162 kb on - strand at 972.167 kb on + strand at 972.167 kb on + strand at 972.168 kb on - strand at 972.168 kb on - strand at 972.168 kb on - strand at 972.227 kb on + strand at 972.228 kb on - strand at 972.228 kb on - strand at 972.228 kb on - strand at 972.241 kb on - strand at 972.316 kb on + strand at 972.317 kb on - strand at 972.334 kb on + strand at 972.453 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.488 kb on + strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.489 kb on - strand, within Pf1N1B4_844 at 972.630 kb on - strand, within Pf1N1B4_844 at 973.237 kb on + strand, within Pf1N1B4_844 at 973.238 kb on - strand, within Pf1N1B4_844 at 973.238 kb on - strand, within Pf1N1B4_844 at 973.636 kb on - strand at 973.636 kb on - strand at 973.726 kb on - strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.775 kb on + strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.776 kb on - strand at 973.862 kb on + strand, within Pf1N1B4_845 at 973.863 kb on - strand, within Pf1N1B4_845 at 973.863 kb on - strand, within Pf1N1B4_845 at 973.935 kb on + strand, within Pf1N1B4_845 at 973.935 kb on + strand at 974.003 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.215 kb on + strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.216 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.304 kb on - strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.368 kb on + strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.369 kb on - strand, within Pf1N1B4_845 at 974.461 kb on + strand at 974.461 kb on + strand at 974.462 kb on - strand at 974.462 kb on - strand at 974.656 kb on - strand at 974.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 971,367 + +2.1 971,372 + -0.5 971,540 + Pf1N1B4_843 0.26 -0.1 971,541 - Pf1N1B4_843 0.26 -0.1 971,600 + Pf1N1B4_843 0.32 +0.1 971,600 + Pf1N1B4_843 0.32 -0.2 971,601 - Pf1N1B4_843 0.32 +0.8 971,601 - Pf1N1B4_843 0.32 -0.3 971,634 + Pf1N1B4_843 0.36 +0.5 971,784 + Pf1N1B4_843 0.52 -1.7 971,784 + Pf1N1B4_843 0.52 -1.5 971,784 + Pf1N1B4_843 0.52 -1.9 971,816 + Pf1N1B4_843 0.55 +1.0 971,816 + -1.9 971,922 + Pf1N1B4_843 0.66 +2.3 971,922 + Pf1N1B4_843 0.66 +2.5 971,922 + Pf1N1B4_843 0.66 +2.7 971,922 + Pf1N1B4_843 0.66 +0.1 971,923 - Pf1N1B4_843 0.66 -0.2 972,161 + -0.9 972,161 + +3.1 972,161 + +0.3 972,161 + -1.8 972,162 - -2.0 972,162 - +0.4 972,167 + +0.5 972,167 + -0.5 972,168 - +1.4 972,168 - +2.1 972,168 - -2.2 972,227 + +0.1 972,228 - -1.0 972,228 - +1.9 972,228 - -0.5 972,241 - -0.1 972,316 + +1.1 972,317 - +0.1 972,334 + -1.6 972,453 + Pf1N1B4_844 0.11 +0.3 972,488 + Pf1N1B4_844 0.13 +0.4 972,488 + Pf1N1B4_844 0.13 -1.2 972,488 + Pf1N1B4_844 0.13 -1.9 972,488 + Pf1N1B4_844 0.13 -2.1 972,488 + Pf1N1B4_844 0.13 -1.0 972,488 + Pf1N1B4_844 0.13 -1.3 972,489 - Pf1N1B4_844 0.13 +0.8 972,489 - Pf1N1B4_844 0.13 -1.0 972,489 - Pf1N1B4_844 0.13 -0.5 972,489 - Pf1N1B4_844 0.13 -1.8 972,489 - Pf1N1B4_844 0.13 -0.2 972,489 - Pf1N1B4_844 0.13 -1.2 972,489 - Pf1N1B4_844 0.13 -0.1 972,630 - Pf1N1B4_844 0.23 +1.8 973,237 + Pf1N1B4_844 0.66 -1.5 973,238 - Pf1N1B4_844 0.67 -1.9 973,238 - Pf1N1B4_844 0.67 -1.2 973,636 - -0.5 973,636 - +1.1 973,726 - +0.9 973,775 + +0.1 973,775 + -0.7 973,775 + -1.2 973,775 + -1.0 973,775 + +1.9 973,775 + -0.5 973,775 + +0.5 973,775 + -2.2 973,775 + +0.5 973,775 + -0.7 973,775 + -1.7 973,775 + -1.2 973,775 + -0.1 973,776 - -0.9 973,776 - +0.7 973,776 - +0.3 973,776 - -1.5 973,776 - +0.1 973,776 - +1.5 973,776 - +0.1 973,776 - +0.1 973,776 - -0.1 973,776 - -0.5 973,862 + Pf1N1B4_845 0.18 -0.7 973,863 - Pf1N1B4_845 0.18 +1.1 973,863 - Pf1N1B4_845 0.18 +0.1 973,935 + Pf1N1B4_845 0.27 +1.1 973,935 + +0.5 974,003 + Pf1N1B4_845 0.35 +1.5 974,215 + Pf1N1B4_845 0.61 +1.1 974,215 + Pf1N1B4_845 0.61 -1.2 974,215 + Pf1N1B4_845 0.61 -1.7 974,216 - Pf1N1B4_845 0.61 +2.1 974,216 - Pf1N1B4_845 0.61 -1.2 974,216 - Pf1N1B4_845 0.61 -0.1 974,216 - Pf1N1B4_845 0.61 +0.4 974,216 - Pf1N1B4_845 0.61 -1.2 974,216 - Pf1N1B4_845 0.61 +2.0 974,304 - Pf1N1B4_845 0.72 +1.0 974,304 - Pf1N1B4_845 0.72 -0.0 974,304 - Pf1N1B4_845 0.72 -0.3 974,368 + Pf1N1B4_845 0.80 -0.5 974,368 + Pf1N1B4_845 0.80 +0.5 974,368 + Pf1N1B4_845 0.80 +0.0 974,369 - Pf1N1B4_845 0.80 +0.6 974,369 - Pf1N1B4_845 0.80 +0.5 974,461 + -1.2 974,461 + +0.7 974,462 - -1.7 974,462 - +0.7 974,656 - +0.4 974,656 - -0.9
Or see this region's nucleotide sequence