Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_623 and Pf1N1B4_624 are separated by 379 nucleotides Pf1N1B4_624 and Pf1N1B4_625 are separated by 95 nucleotides Pf1N1B4_625 and Pf1N1B4_626 are separated by 135 nucleotides
Pf1N1B4_623 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 721,995 to 723,119
_623
Pf1N1B4_624 - Cold shock protein CspA, at 723,499 to 723,711
_624
Pf1N1B4_625 - FIG023103: Predicted transmembrane protein, at 723,807 to 724,295
_625
Pf1N1B4_626 - Quinolinate synthetase (EC 2.5.1.72), at 724,431 to 725,489
_626
Position (kb)
723
724
725 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 722.892 kb on - strand, within Pf1N1B4_623 at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.015 kb on + strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.016 kb on - strand at 723.076 kb on + strand at 723.105 kb on + strand at 723.105 kb on + strand at 723.106 kb on - strand at 723.108 kb on + strand at 723.131 kb on + strand at 723.131 kb on + strand at 723.132 kb on - strand at 723.132 kb on - strand at 723.132 kb on - strand at 723.133 kb on + strand at 723.134 kb on - strand at 723.134 kb on - strand at 723.219 kb on + strand at 723.219 kb on + strand at 723.219 kb on + strand at 723.219 kb on + strand at 723.220 kb on - strand at 723.221 kb on + strand at 723.266 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.388 kb on + strand at 723.389 kb on - strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.395 kb on + strand at 723.396 kb on - strand at 723.399 kb on + strand at 723.399 kb on + strand at 723.400 kb on - strand at 723.400 kb on - strand at 723.421 kb on + strand at 723.422 kb on - strand at 723.445 kb on - strand at 723.695 kb on + strand at 723.695 kb on + strand at 723.695 kb on + strand at 723.737 kb on + strand at 723.766 kb on + strand at 723.766 kb on + strand at 723.767 kb on - strand at 723.775 kb on - strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.823 kb on + strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.824 kb on - strand at 723.882 kb on + strand, within Pf1N1B4_625 at 723.883 kb on - strand, within Pf1N1B4_625 at 723.883 kb on - strand, within Pf1N1B4_625 at 723.883 kb on - strand, within Pf1N1B4_625 at 723.896 kb on - strand, within Pf1N1B4_625 at 723.896 kb on - strand, within Pf1N1B4_625 at 723.930 kb on + strand, within Pf1N1B4_625 at 723.930 kb on + strand, within Pf1N1B4_625 at 723.931 kb on - strand, within Pf1N1B4_625 at 723.931 kb on - strand, within Pf1N1B4_625 at 723.931 kb on - strand, within Pf1N1B4_625 at 724.016 kb on - strand, within Pf1N1B4_625 at 724.186 kb on + strand, within Pf1N1B4_625 at 724.187 kb on - strand, within Pf1N1B4_625 at 724.251 kb on + strand at 724.251 kb on + strand at 724.251 kb on + strand at 724.251 kb on + strand at 724.251 kb on + strand at 724.251 kb on + strand at 724.251 kb on + strand at 724.251 kb on + strand at 724.252 kb on - strand at 724.252 kb on - strand at 724.252 kb on - strand at 724.252 kb on - strand at 724.252 kb on - strand at 724.252 kb on - strand at 724.270 kb on + strand at 724.270 kb on + strand at 724.270 kb on + strand at 724.347 kb on + strand at 724.348 kb on - strand at 724.348 kb on - strand at 724.348 kb on - strand at 724.610 kb on + strand, within Pf1N1B4_626 at 724.935 kb on - strand, within Pf1N1B4_626 at 724.936 kb on + strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 724.937 kb on - strand, within Pf1N1B4_626 at 725.072 kb on - strand at 725.072 kb on - strand, within Pf1N1B4_626 at 725.072 kb on - strand, within Pf1N1B4_626 at 725.084 kb on + strand, within Pf1N1B4_626 at 725.084 kb on + strand, within Pf1N1B4_626 at 725.085 kb on - strand, within Pf1N1B4_626 at 725.085 kb on - strand, within Pf1N1B4_626
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 722,892 - Pf1N1B4_623 0.80 +0.0 723,015 + +0.0 723,015 + +1.4 723,015 + +0.3 723,015 + -0.6 723,015 + +0.4 723,015 + -1.7 723,015 + -2.1 723,015 + +1.2 723,015 + -1.0 723,015 + +1.6 723,016 - +1.1 723,016 - +0.6 723,016 - +0.0 723,016 - -0.3 723,016 - -0.1 723,016 - -0.8 723,076 + -0.8 723,105 + -0.5 723,105 + -0.3 723,106 - -1.6 723,108 + +0.7 723,131 + -1.0 723,131 + -0.1 723,132 - +2.6 723,132 - -0.6 723,132 - +2.0 723,133 + -0.7 723,134 - -2.3 723,134 - +0.4 723,219 + -0.6 723,219 + -1.6 723,219 + +0.0 723,219 + +0.8 723,220 - -0.3 723,221 + -0.4 723,266 + -1.6 723,388 + +0.0 723,388 + +1.0 723,388 + +1.0 723,388 + +1.4 723,388 + +1.0 723,388 + +0.0 723,389 - -0.0 723,395 + +2.8 723,395 + -1.0 723,395 + -1.8 723,395 + +0.0 723,395 + +0.8 723,396 - -0.6 723,399 + -0.6 723,399 + -1.3 723,400 - -0.6 723,400 - -0.6 723,421 + -0.6 723,422 - +1.8 723,445 - +3.0 723,695 + -0.3 723,695 + -0.8 723,695 + +2.4 723,737 + +1.6 723,766 + +0.2 723,766 + +1.0 723,767 - +0.6 723,775 - +1.3 723,823 + -1.7 723,823 + -0.6 723,823 + -1.3 723,823 + -0.6 723,823 + +0.4 723,823 + +0.4 723,823 + -1.3 723,823 + -0.6 723,823 + +0.6 723,823 + -0.7 723,823 + -0.2 723,823 + +0.6 723,824 - +1.4 723,824 - +0.4 723,824 - -0.7 723,824 - +0.3 723,824 - +0.5 723,824 - +1.0 723,824 - -0.6 723,824 - -1.6 723,824 - -1.0 723,824 - -0.3 723,882 + Pf1N1B4_625 0.15 -0.1 723,883 - Pf1N1B4_625 0.16 -0.8 723,883 - Pf1N1B4_625 0.16 +0.0 723,883 - Pf1N1B4_625 0.16 -1.0 723,896 - Pf1N1B4_625 0.18 +1.0 723,896 - Pf1N1B4_625 0.18 -1.8 723,930 + Pf1N1B4_625 0.25 +1.4 723,930 + Pf1N1B4_625 0.25 -0.6 723,931 - Pf1N1B4_625 0.25 -1.3 723,931 - Pf1N1B4_625 0.25 -3.0 723,931 - Pf1N1B4_625 0.25 -0.6 724,016 - Pf1N1B4_625 0.43 -1.4 724,186 + Pf1N1B4_625 0.78 +0.1 724,187 - Pf1N1B4_625 0.78 -1.1 724,251 + +0.0 724,251 + +0.7 724,251 + -0.6 724,251 + -1.0 724,251 + -1.3 724,251 + -0.5 724,251 + +0.7 724,251 + -4.0 724,252 - -0.3 724,252 - +0.0 724,252 - +0.0 724,252 - -2.1 724,252 - -0.3 724,252 - +0.5 724,270 + -0.6 724,270 + -1.3 724,270 + -0.3 724,347 + +2.5 724,348 - +0.3 724,348 - -0.6 724,348 - -0.6 724,610 + Pf1N1B4_626 0.17 +0.0 724,935 - Pf1N1B4_626 0.48 -1.3 724,936 + Pf1N1B4_626 0.48 +1.0 724,937 - Pf1N1B4_626 0.48 +0.8 724,937 - Pf1N1B4_626 0.48 +0.3 724,937 - Pf1N1B4_626 0.48 -0.0 724,937 - Pf1N1B4_626 0.48 +1.3 724,937 - Pf1N1B4_626 0.48 +0.0 724,937 - Pf1N1B4_626 0.48 -1.3 724,937 - Pf1N1B4_626 0.48 -0.3 724,937 - Pf1N1B4_626 0.48 -0.2 724,937 - Pf1N1B4_626 0.48 +2.8 724,937 - Pf1N1B4_626 0.48 -2.0 724,937 - Pf1N1B4_626 0.48 -0.7 724,937 - Pf1N1B4_626 0.48 +0.8 725,072 - +0.0 725,072 - Pf1N1B4_626 0.61 +0.4 725,072 - Pf1N1B4_626 0.61 +0.4 725,084 + Pf1N1B4_626 0.62 -1.2 725,084 + Pf1N1B4_626 0.62 -0.4 725,085 - Pf1N1B4_626 0.62 -0.7 725,085 - Pf1N1B4_626 0.62 -0.8
Or see this region's nucleotide sequence