Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_604

Experiment: methylglyoxal 0.008 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_602 and Pf1N1B4_603 are separated by 55 nucleotidesPf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotidesPf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotidesPf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotides Pf1N1B4_602 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 695,951 to 696,952 _602 Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472 _603 Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068 _604 Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288 _605 Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128 _606 Position (kb) 697 698 699Strain fitness (log2 ratio) -2 -1 0 1 2 3at 696.950 kb on + strandat 697.088 kb on - strandat 697.389 kb on + strand, within Pf1N1B4_603at 697.389 kb on + strand, within Pf1N1B4_603at 697.389 kb on + strand, within Pf1N1B4_603at 697.390 kb on - strand, within Pf1N1B4_603at 697.390 kb on - strand, within Pf1N1B4_603at 697.400 kb on + strand, within Pf1N1B4_603at 697.400 kb on + strand, within Pf1N1B4_603at 697.401 kb on - strand, within Pf1N1B4_603at 697.401 kb on - strand, within Pf1N1B4_603at 697.444 kb on - strandat 697.452 kb on + strandat 697.452 kb on + strandat 697.452 kb on + strandat 697.452 kb on + strandat 697.453 kb on - strandat 697.453 kb on - strandat 697.453 kb on - strandat 697.471 kb on - strandat 697.664 kb on + strand, within Pf1N1B4_604at 697.665 kb on - strand, within Pf1N1B4_604at 697.665 kb on - strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.958 kb on + strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 697.959 kb on - strand, within Pf1N1B4_604at 698.098 kb on + strandat 698.098 kb on + strandat 698.635 kb on - strand, within Pf1N1B4_606at 698.840 kb on + strand, within Pf1N1B4_606at 698.840 kb on + strand, within Pf1N1B4_606at 698.974 kb on - strand, within Pf1N1B4_606at 699.030 kb on - strand, within Pf1N1B4_606

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol%
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696,950 + -2.0
697,088 - +0.4
697,389 + Pf1N1B4_603 0.82 -0.4
697,389 + Pf1N1B4_603 0.82 +1.0
697,389 + Pf1N1B4_603 0.82 +0.3
697,390 - Pf1N1B4_603 0.82 -0.3
697,390 - Pf1N1B4_603 0.82 -1.0
697,400 + Pf1N1B4_603 0.84 +0.8
697,400 + Pf1N1B4_603 0.84 -1.0
697,401 - Pf1N1B4_603 0.85 +1.5
697,401 - Pf1N1B4_603 0.85 -0.2
697,444 - -1.6
697,452 + +1.6
697,452 + -0.8
697,452 + +0.6
697,452 + -2.0
697,453 - +1.4
697,453 - -2.2
697,453 - -1.3
697,471 - +0.0
697,664 + Pf1N1B4_604 0.22 -0.2
697,665 - Pf1N1B4_604 0.22 +0.7
697,665 - Pf1N1B4_604 0.22 -1.0
697,958 + Pf1N1B4_604 0.79 +0.3
697,958 + Pf1N1B4_604 0.79 +3.0
697,958 + Pf1N1B4_604 0.79 +0.4
697,958 + Pf1N1B4_604 0.79 +0.4
697,959 - Pf1N1B4_604 0.79 -0.8
697,959 - Pf1N1B4_604 0.79 +0.9
697,959 - Pf1N1B4_604 0.79 +0.6
697,959 - Pf1N1B4_604 0.79 -1.8
698,098 + +2.3
698,098 + -0.6
698,635 - Pf1N1B4_606 0.46 -0.7
698,840 + Pf1N1B4_606 0.68 -1.0
698,840 + Pf1N1B4_606 0.68 -1.3
698,974 - Pf1N1B4_606 0.83 -1.3
699,030 - Pf1N1B4_606 0.89 -2.6

Or see this region's nucleotide sequence