Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_602 and Pf1N1B4_603 are separated by 55 nucleotides Pf1N1B4_603 and Pf1N1B4_604 are separated by 77 nucleotides Pf1N1B4_604 and Pf1N1B4_605 are separated by 28 nucleotides Pf1N1B4_605 and Pf1N1B4_606 overlap by 69 nucleotides
Pf1N1B4_602 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 695,951 to 696,952
_602
Pf1N1B4_603 - Methylglyoxal synthase (EC 4.2.3.3), at 697,008 to 697,472
_603
Pf1N1B4_604 - FIG006045: Sigma factor, ECF subfamily, at 697,550 to 698,068
_604
Pf1N1B4_605 - hypothetical protein, at 698,097 to 698,288
_605
Pf1N1B4_606 - Iron siderophore sensor protein, at 698,220 to 699,128
_606
Position (kb)
697
698
699 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 696.950 kb on + strand at 697.088 kb on - strand at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.389 kb on + strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.390 kb on - strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.400 kb on + strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.401 kb on - strand, within Pf1N1B4_603 at 697.444 kb on - strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.452 kb on + strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.453 kb on - strand at 697.471 kb on - strand at 697.664 kb on + strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.665 kb on - strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.958 kb on + strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 697.959 kb on - strand, within Pf1N1B4_604 at 698.098 kb on + strand at 698.098 kb on + strand at 698.635 kb on - strand, within Pf1N1B4_606 at 698.840 kb on + strand, within Pf1N1B4_606 at 698.840 kb on + strand, within Pf1N1B4_606 at 698.974 kb on - strand, within Pf1N1B4_606 at 699.030 kb on - strand, within Pf1N1B4_606
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 696,950 + -2.0 697,088 - +0.4 697,389 + Pf1N1B4_603 0.82 -0.4 697,389 + Pf1N1B4_603 0.82 +1.0 697,389 + Pf1N1B4_603 0.82 +0.3 697,390 - Pf1N1B4_603 0.82 -0.3 697,390 - Pf1N1B4_603 0.82 -1.0 697,400 + Pf1N1B4_603 0.84 +0.8 697,400 + Pf1N1B4_603 0.84 -1.0 697,401 - Pf1N1B4_603 0.85 +1.5 697,401 - Pf1N1B4_603 0.85 -0.2 697,444 - -1.6 697,452 + +1.6 697,452 + -0.8 697,452 + +0.6 697,452 + -2.0 697,453 - +1.4 697,453 - -2.2 697,453 - -1.3 697,471 - +0.0 697,664 + Pf1N1B4_604 0.22 -0.2 697,665 - Pf1N1B4_604 0.22 +0.7 697,665 - Pf1N1B4_604 0.22 -1.0 697,958 + Pf1N1B4_604 0.79 +0.3 697,958 + Pf1N1B4_604 0.79 +3.0 697,958 + Pf1N1B4_604 0.79 +0.4 697,958 + Pf1N1B4_604 0.79 +0.4 697,959 - Pf1N1B4_604 0.79 -0.8 697,959 - Pf1N1B4_604 0.79 +0.9 697,959 - Pf1N1B4_604 0.79 +0.6 697,959 - Pf1N1B4_604 0.79 -1.8 698,098 + +2.3 698,098 + -0.6 698,635 - Pf1N1B4_606 0.46 -0.7 698,840 + Pf1N1B4_606 0.68 -1.0 698,840 + Pf1N1B4_606 0.68 -1.3 698,974 - Pf1N1B4_606 0.83 -1.3 699,030 - Pf1N1B4_606 0.89 -2.6
Or see this region's nucleotide sequence