Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6029 and Pf1N1B4_6030 are separated by 33 nucleotides Pf1N1B4_6030 and Pf1N1B4_6031 overlap by 4 nucleotides Pf1N1B4_6031 and Pf1N1B4_6032 are separated by 56 nucleotides
Pf1N1B4_6029 - uridine nucleosidase (EC 3.2.2.3) <small>(from data)</small>, at 493,499 to 494,527
_6029
Pf1N1B4_6030 - Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1), at 494,561 to 494,965
_6030
Pf1N1B4_6031 - Ribokinase (EC 2.7.1.15) <small>(from data)</small>, at 494,962 to 495,879
_6031
Pf1N1B4_6032 - Ribose operon repressor, at 495,936 to 496,955
_6032
Position (kb) 494
495
496 Strain fitness (log2 ratio) -2
-1
0
1
2 at 494.118 kb on + strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.119 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.209 kb on - strand at 494.209 kb on - strand, within Pf1N1B4_6029 at 494.351 kb on - strand, within Pf1N1B4_6029 at 494.517 kb on + strand at 494.518 kb on - strand at 494.518 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.563 kb on - strand at 494.571 kb on - strand at 494.571 kb on - strand at 494.599 kb on - strand at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.792 kb on + strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.793 kb on - strand, within Pf1N1B4_6030 at 494.806 kb on + strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.807 kb on - strand, within Pf1N1B4_6030 at 494.974 kb on - strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.983 kb on + strand at 494.984 kb on - strand at 494.984 kb on - strand at 494.984 kb on - strand at 495.493 kb on + strand, within Pf1N1B4_6031 at 495.494 kb on - strand, within Pf1N1B4_6031 at 495.681 kb on - strand, within Pf1N1B4_6031 at 495.857 kb on - strand at 495.857 kb on - strand at 495.857 kb on - strand at 495.860 kb on - strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.879 kb on + strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.880 kb on - strand at 495.927 kb on - strand at 495.927 kb on - strand at 495.992 kb on + strand at 495.993 kb on - strand at 495.993 kb on - strand at 496.179 kb on - strand, within Pf1N1B4_6032 at 496.179 kb on - strand, within Pf1N1B4_6032 at 496.267 kb on - strand, within Pf1N1B4_6032 at 496.471 kb on + strand, within Pf1N1B4_6032 at 496.636 kb on + strand, within Pf1N1B4_6032 at 496.636 kb on + strand, within Pf1N1B4_6032 at 496.688 kb on - strand, within Pf1N1B4_6032 at 496.706 kb on - strand, within Pf1N1B4_6032 at 496.706 kb on - strand, within Pf1N1B4_6032 at 496.822 kb on + strand, within Pf1N1B4_6032 at 496.823 kb on - strand, within Pf1N1B4_6032 at 496.860 kb on + strand at 496.861 kb on - strand at 496.861 kb on - strand at 496.861 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 494,118 + Pf1N1B4_6029 0.60 +0.2 494,119 - Pf1N1B4_6029 0.60 -2.4 494,119 - Pf1N1B4_6029 0.60 +0.3 494,209 - Pf1N1B4_6029 0.69 -0.6 494,209 - -0.4 494,209 - Pf1N1B4_6029 0.69 -0.5 494,351 - Pf1N1B4_6029 0.83 -1.4 494,517 + +0.6 494,518 - -1.1 494,518 - +0.9 494,563 - -0.2 494,563 - -2.0 494,563 - -0.8 494,571 - -0.4 494,571 - -0.4 494,599 - +0.6 494,792 + Pf1N1B4_6030 0.57 +2.7 494,792 + Pf1N1B4_6030 0.57 -1.4 494,792 + Pf1N1B4_6030 0.57 -0.1 494,792 + Pf1N1B4_6030 0.57 -1.0 494,793 - Pf1N1B4_6030 0.57 -0.4 494,793 - Pf1N1B4_6030 0.57 +0.1 494,806 + Pf1N1B4_6030 0.60 +0.6 494,807 - Pf1N1B4_6030 0.61 -0.4 494,807 - Pf1N1B4_6030 0.61 +0.6 494,974 - -0.7 494,983 + -1.7 494,983 + -1.0 494,983 + +0.2 494,984 - +0.3 494,984 - -0.4 494,984 - -2.0 495,493 + Pf1N1B4_6031 0.58 +0.6 495,494 - Pf1N1B4_6031 0.58 -1.4 495,681 - Pf1N1B4_6031 0.78 -1.4 495,857 - +1.9 495,857 - +0.4 495,857 - -0.3 495,860 - -1.0 495,879 + -0.8 495,879 + -0.7 495,879 + +2.2 495,879 + +1.8 495,880 - +0.8 495,880 - -0.5 495,880 - -0.1 495,880 - -2.2 495,880 - -1.0 495,880 - -0.8 495,880 - -2.2 495,880 - +0.2 495,880 - -1.2 495,880 - -1.4 495,880 - -1.0 495,880 - +1.0 495,880 - -0.4 495,880 - +1.4 495,880 - +0.6 495,880 - -0.4 495,880 - -0.1 495,880 - -0.3 495,880 - -1.0 495,880 - -1.1 495,927 - -0.9 495,927 - -0.5 495,992 + -0.4 495,993 - +0.6 495,993 - +0.8 496,179 - Pf1N1B4_6032 0.24 +0.2 496,179 - Pf1N1B4_6032 0.24 -2.2 496,267 - Pf1N1B4_6032 0.32 +0.6 496,471 + Pf1N1B4_6032 0.52 +0.1 496,636 + Pf1N1B4_6032 0.69 -0.6 496,636 + Pf1N1B4_6032 0.69 -1.2 496,688 - Pf1N1B4_6032 0.74 -0.4 496,706 - Pf1N1B4_6032 0.75 -0.1 496,706 - Pf1N1B4_6032 0.75 -0.7 496,822 + Pf1N1B4_6032 0.87 +0.0 496,823 - Pf1N1B4_6032 0.87 -0.4 496,860 + +0.0 496,861 - +1.1 496,861 - -0.4 496,861 - +0.4
Or see this region's nucleotide sequence