Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5998 and Pf1N1B4_5999 are separated by 230 nucleotides Pf1N1B4_5999 and Pf1N1B4_6000 are separated by 111 nucleotides
Pf1N1B4_5998 - Quinone oxidoreductase (EC 1.6.5.5), at 449,129 to 450,109
_5998
Pf1N1B4_5999 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 450,340 to 451,842
_5999
Pf1N1B4_6000 - Putative L-xylulose-5-phosphate 3-epimerase, at 451,954 to 453,024
_6000
Position (kb)
450
451
452 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 449.532 kb on + strand at 449.532 kb on + strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.533 kb on - strand, within Pf1N1B4_5998 at 449.718 kb on + strand, within Pf1N1B4_5998 at 449.824 kb on - strand, within Pf1N1B4_5998 at 449.836 kb on + strand, within Pf1N1B4_5998 at 449.837 kb on - strand, within Pf1N1B4_5998 at 449.837 kb on - strand, within Pf1N1B4_5998 at 449.853 kb on + strand at 449.854 kb on - strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand, within Pf1N1B4_5998 at 449.876 kb on + strand at 449.877 kb on - strand, within Pf1N1B4_5998 at 449.973 kb on + strand, within Pf1N1B4_5998 at 449.973 kb on + strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 449.974 kb on - strand, within Pf1N1B4_5998 at 450.169 kb on + strand at 450.169 kb on + strand at 450.264 kb on - strand at 450.338 kb on + strand at 450.338 kb on + strand at 450.338 kb on + strand at 450.338 kb on + strand at 450.338 kb on + strand at 450.339 kb on - strand at 450.339 kb on - strand at 450.418 kb on + strand at 450.418 kb on + strand at 450.589 kb on + strand, within Pf1N1B4_5999 at 450.589 kb on + strand, within Pf1N1B4_5999 at 450.589 kb on + strand, within Pf1N1B4_5999 at 450.590 kb on - strand, within Pf1N1B4_5999 at 450.590 kb on - strand, within Pf1N1B4_5999 at 450.590 kb on - strand, within Pf1N1B4_5999 at 450.590 kb on - strand, within Pf1N1B4_5999 at 450.590 kb on - strand, within Pf1N1B4_5999 at 450.590 kb on - strand, within Pf1N1B4_5999 at 450.712 kb on + strand at 450.713 kb on - strand, within Pf1N1B4_5999 at 450.762 kb on + strand, within Pf1N1B4_5999 at 450.762 kb on + strand, within Pf1N1B4_5999 at 450.762 kb on + strand, within Pf1N1B4_5999 at 450.762 kb on + strand, within Pf1N1B4_5999 at 450.766 kb on + strand, within Pf1N1B4_5999 at 450.766 kb on + strand, within Pf1N1B4_5999 at 450.892 kb on + strand, within Pf1N1B4_5999 at 450.984 kb on - strand, within Pf1N1B4_5999 at 451.007 kb on + strand, within Pf1N1B4_5999 at 451.008 kb on - strand, within Pf1N1B4_5999 at 451.105 kb on - strand, within Pf1N1B4_5999 at 451.192 kb on + strand, within Pf1N1B4_5999 at 451.192 kb on + strand, within Pf1N1B4_5999 at 451.192 kb on + strand, within Pf1N1B4_5999 at 451.304 kb on - strand, within Pf1N1B4_5999 at 451.334 kb on - strand, within Pf1N1B4_5999 at 451.334 kb on - strand, within Pf1N1B4_5999 at 451.371 kb on - strand, within Pf1N1B4_5999 at 451.382 kb on - strand, within Pf1N1B4_5999 at 451.382 kb on - strand, within Pf1N1B4_5999 at 451.531 kb on + strand, within Pf1N1B4_5999 at 451.696 kb on + strand at 451.696 kb on + strand at 451.697 kb on - strand at 451.697 kb on - strand at 451.697 kb on - strand at 451.697 kb on - strand at 451.847 kb on - strand at 451.861 kb on - strand at 451.861 kb on - strand at 451.868 kb on - strand at 451.868 kb on - strand at 451.868 kb on - strand at 451.883 kb on + strand at 451.884 kb on - strand at 451.884 kb on - strand at 451.962 kb on + strand at 451.963 kb on - strand at 451.963 kb on - strand at 452.431 kb on + strand, within Pf1N1B4_6000 at 452.624 kb on + strand, within Pf1N1B4_6000
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 449,532 + -2.0 449,532 + Pf1N1B4_5998 0.41 +0.1 449,533 - Pf1N1B4_5998 0.41 +0.3 449,533 - Pf1N1B4_5998 0.41 -0.9 449,533 - Pf1N1B4_5998 0.41 -0.3 449,533 - Pf1N1B4_5998 0.41 -1.2 449,718 + Pf1N1B4_5998 0.60 +1.4 449,824 - Pf1N1B4_5998 0.71 -1.0 449,836 + Pf1N1B4_5998 0.72 -0.4 449,837 - Pf1N1B4_5998 0.72 -0.1 449,837 - Pf1N1B4_5998 0.72 +0.6 449,853 + -0.4 449,854 - Pf1N1B4_5998 0.74 -0.1 449,876 + Pf1N1B4_5998 0.76 +0.2 449,876 + +0.0 449,876 + Pf1N1B4_5998 0.76 -2.4 449,876 + Pf1N1B4_5998 0.76 -0.4 449,876 + Pf1N1B4_5998 0.76 -0.1 449,876 + -1.4 449,877 - Pf1N1B4_5998 0.76 +0.8 449,973 + Pf1N1B4_5998 0.86 +0.4 449,973 + Pf1N1B4_5998 0.86 +0.1 449,974 - Pf1N1B4_5998 0.86 +0.5 449,974 - Pf1N1B4_5998 0.86 -0.4 449,974 - Pf1N1B4_5998 0.86 -1.0 450,169 + -0.4 450,169 + +2.4 450,264 - -1.7 450,338 + -0.3 450,338 + -1.1 450,338 + -1.7 450,338 + +1.6 450,338 + -0.4 450,339 - +0.6 450,339 - +0.6 450,418 + +0.9 450,418 + -0.1 450,589 + Pf1N1B4_5999 0.17 +0.4 450,589 + Pf1N1B4_5999 0.17 -0.6 450,589 + Pf1N1B4_5999 0.17 -1.7 450,590 - Pf1N1B4_5999 0.17 +0.6 450,590 - Pf1N1B4_5999 0.17 +0.2 450,590 - Pf1N1B4_5999 0.17 +0.6 450,590 - Pf1N1B4_5999 0.17 +0.6 450,590 - Pf1N1B4_5999 0.17 +1.3 450,590 - Pf1N1B4_5999 0.17 +0.9 450,712 + +0.6 450,713 - Pf1N1B4_5999 0.25 -0.4 450,762 + Pf1N1B4_5999 0.28 +0.0 450,762 + Pf1N1B4_5999 0.28 -1.0 450,762 + Pf1N1B4_5999 0.28 +0.6 450,762 + Pf1N1B4_5999 0.28 -2.6 450,766 + Pf1N1B4_5999 0.28 -0.9 450,766 + Pf1N1B4_5999 0.28 +1.9 450,892 + Pf1N1B4_5999 0.37 -0.6 450,984 - Pf1N1B4_5999 0.43 -2.6 451,007 + Pf1N1B4_5999 0.44 -0.5 451,008 - Pf1N1B4_5999 0.44 -2.2 451,105 - Pf1N1B4_5999 0.51 -2.0 451,192 + Pf1N1B4_5999 0.57 -0.4 451,192 + Pf1N1B4_5999 0.57 -2.6 451,192 + Pf1N1B4_5999 0.57 +0.0 451,304 - Pf1N1B4_5999 0.64 -1.2 451,334 - Pf1N1B4_5999 0.66 -1.4 451,334 - Pf1N1B4_5999 0.66 +1.4 451,371 - Pf1N1B4_5999 0.69 +1.6 451,382 - Pf1N1B4_5999 0.69 +1.4 451,382 - Pf1N1B4_5999 0.69 -1.7 451,531 + Pf1N1B4_5999 0.79 +0.0 451,696 + -2.6 451,696 + +0.9 451,697 - +1.9 451,697 - -1.4 451,697 - -0.4 451,697 - -1.0 451,847 - +2.8 451,861 - +2.4 451,861 - -0.1 451,868 - -2.6 451,868 - -0.0 451,868 - -1.0 451,883 + +0.8 451,884 - +0.1 451,884 - -0.4 451,962 + +0.0 451,963 - +1.1 451,963 - +0.0 452,431 + Pf1N1B4_6000 0.45 +1.6 452,624 + Pf1N1B4_6000 0.63 +0.4
Or see this region's nucleotide sequence