Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5991 and Pf1N1B4_5992 are separated by 300 nucleotides Pf1N1B4_5992 and Pf1N1B4_5993 overlap by 4 nucleotides Pf1N1B4_5993 and Pf1N1B4_5994 are separated by 14 nucleotides Pf1N1B4_5994 and Pf1N1B4_5995 overlap by 4 nucleotides
Pf1N1B4_5991 - Transcriptional regulator, AraC family, at 442,361 to 443,413
_5991
Pf1N1B4_5992 - Inner membrane protein, at 443,714 to 444,724
_5992
Pf1N1B4_5993 - Ferredoxin, 2Fe-2S, at 444,721 to 445,029
_5993
Pf1N1B4_5994 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 445,044 to 445,706
_5994
Pf1N1B4_5995 - Transporter, MFS superfamily, at 445,703 to 447,034
_5995
Position (kb) 443
444
445 Strain fitness (log2 ratio) -2
-1
0
1
2 at 443.013 kb on - strand, within Pf1N1B4_5991 at 443.075 kb on + strand, within Pf1N1B4_5991 at 443.075 kb on + strand, within Pf1N1B4_5991 at 443.075 kb on + strand at 443.076 kb on - strand, within Pf1N1B4_5991 at 443.076 kb on - strand, within Pf1N1B4_5991 at 443.243 kb on + strand at 443.465 kb on + strand at 443.482 kb on - strand at 443.534 kb on - strand at 443.924 kb on - strand, within Pf1N1B4_5992 at 444.515 kb on - strand, within Pf1N1B4_5992 at 444.757 kb on + strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.800 kb on - strand, within Pf1N1B4_5993 at 444.822 kb on - strand, within Pf1N1B4_5993 at 445.357 kb on - strand, within Pf1N1B4_5994 at 445.401 kb on - strand, within Pf1N1B4_5994 at 445.618 kb on - strand, within Pf1N1B4_5994 at 445.688 kb on + strand at 445.689 kb on - strand at 445.692 kb on + strand at 445.693 kb on - strand at 445.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 443,013 - Pf1N1B4_5991 0.62 -1.7 443,075 + Pf1N1B4_5991 0.68 +0.6 443,075 + Pf1N1B4_5991 0.68 -0.4 443,075 + -0.4 443,076 - Pf1N1B4_5991 0.68 +0.1 443,076 - Pf1N1B4_5991 0.68 +1.8 443,243 + +1.0 443,465 + -1.7 443,482 - +1.0 443,534 - -1.9 443,924 - Pf1N1B4_5992 0.21 +1.1 444,515 - Pf1N1B4_5992 0.79 +0.6 444,757 + Pf1N1B4_5993 0.12 -0.4 444,800 - Pf1N1B4_5993 0.26 +0.9 444,800 - Pf1N1B4_5993 0.26 +2.2 444,822 - Pf1N1B4_5993 0.33 -0.6 445,357 - Pf1N1B4_5994 0.47 -0.1 445,401 - Pf1N1B4_5994 0.54 -0.6 445,618 - Pf1N1B4_5994 0.87 -1.2 445,688 + -0.6 445,689 - -1.7 445,692 + -2.0 445,693 - -0.8 445,693 - -0.4
Or see this region's nucleotide sequence