Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5693 and Pf1N1B4_5694 are separated by 13 nucleotides Pf1N1B4_5694 and Pf1N1B4_5695 are separated by 20 nucleotides Pf1N1B4_5695 and Pf1N1B4_5696 are separated by 160 nucleotides Pf1N1B4_5696 and Pf1N1B4_5697 are separated by 360 nucleotides
Pf1N1B4_5693 - Gluconate dehydratase (EC 4.2.1.39), at 131,440 to 132,624
_5693
Pf1N1B4_5694 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 132,638 to 133,438
_5694
Pf1N1B4_5695 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 133,459 to 134,892
_5695
Pf1N1B4_5696 - hypothetical protein, at 135,053 to 135,181
_5696
Pf1N1B4_5697 - putative exported protein, at 135,542 to 136,108
_5697
Position (kb)
133
134
135 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 132.613 kb on + strand at 133.156 kb on + strand, within Pf1N1B4_5694 at 133.433 kb on + strand at 133.434 kb on - strand at 133.783 kb on + strand, within Pf1N1B4_5695 at 133.792 kb on + strand, within Pf1N1B4_5695 at 133.792 kb on + strand, within Pf1N1B4_5695 at 133.793 kb on - strand, within Pf1N1B4_5695 at 133.793 kb on - strand, within Pf1N1B4_5695 at 133.793 kb on - strand at 134.323 kb on + strand, within Pf1N1B4_5695 at 134.324 kb on - strand, within Pf1N1B4_5695 at 134.324 kb on - strand, within Pf1N1B4_5695 at 134.699 kb on - strand, within Pf1N1B4_5695 at 134.758 kb on + strand at 134.857 kb on + strand at 134.858 kb on - strand at 134.878 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.960 kb on - strand at 134.960 kb on - strand at 134.960 kb on - strand at 134.988 kb on + strand at 134.989 kb on - strand at 134.989 kb on - strand at 134.989 kb on - strand at 134.990 kb on + strand at 135.451 kb on + strand at 135.471 kb on + strand at 135.472 kb on - strand at 135.472 kb on - strand at 135.525 kb on + strand at 135.526 kb on - strand at 135.526 kb on - strand at 135.615 kb on + strand, within Pf1N1B4_5697 at 135.615 kb on + strand at 135.616 kb on - strand, within Pf1N1B4_5697 at 135.684 kb on - strand, within Pf1N1B4_5697 at 135.809 kb on + strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 132,613 + -0.0 133,156 + Pf1N1B4_5694 0.65 -1.3 133,433 + +1.5 133,434 - +0.1 133,783 + Pf1N1B4_5695 0.23 -1.5 133,792 + Pf1N1B4_5695 0.23 +1.3 133,792 + Pf1N1B4_5695 0.23 +1.0 133,793 - Pf1N1B4_5695 0.23 +1.4 133,793 - Pf1N1B4_5695 0.23 -0.9 133,793 - +1.9 134,323 + Pf1N1B4_5695 0.60 -2.6 134,324 - Pf1N1B4_5695 0.60 -0.9 134,324 - Pf1N1B4_5695 0.60 -1.8 134,699 - Pf1N1B4_5695 0.86 -1.2 134,758 + +1.9 134,857 + -1.5 134,858 - -2.3 134,878 + -1.0 134,959 + -1.0 134,959 + +2.4 134,959 + +1.1 134,959 + +0.1 134,959 + +1.5 134,959 + -1.6 134,960 - +0.1 134,960 - +0.2 134,960 - -1.0 134,988 + +1.1 134,989 - +0.5 134,989 - +1.1 134,989 - +1.4 134,990 + -0.5 135,451 + -0.0 135,471 + -1.0 135,472 - -1.3 135,472 - -1.3 135,525 + -1.8 135,526 - -0.5 135,526 - -1.0 135,615 + Pf1N1B4_5697 0.13 +1.3 135,615 + -1.0 135,616 - Pf1N1B4_5697 0.13 -0.0 135,684 - Pf1N1B4_5697 0.25 +0.2 135,809 + Pf1N1B4_5697 0.47 +0.4 135,812 - Pf1N1B4_5697 0.48 +0.5 135,812 - Pf1N1B4_5697 0.48 +0.5 135,812 - Pf1N1B4_5697 0.48 +0.0 135,812 - Pf1N1B4_5697 0.48 -2.9 135,812 - Pf1N1B4_5697 0.48 -0.9 135,812 - Pf1N1B4_5697 0.48 +2.1
Or see this region's nucleotide sequence