Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5587 and Pf1N1B4_5588 are separated by 10 nucleotides Pf1N1B4_5588 and Pf1N1B4_5589 are separated by 83 nucleotides Pf1N1B4_5589 and Pf1N1B4_5590 overlap by 4 nucleotides
Pf1N1B4_5587 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 8,787 to 9,737
_5587
Pf1N1B4_5588 - Transcriptional regulator for ferulate or vanillate catabolism, at 9,748 to 10,461
_5588
Pf1N1B4_5589 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 10,545 to 11,786
_5589
Pf1N1B4_5590 - Trans-feruloyl-CoA synthase (EC 6.2.1.34), at 11,783 to 13,666
_5590
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 9.547 kb on + strand, within Pf1N1B4_5587 at 9.548 kb on - strand, within Pf1N1B4_5587 at 9.739 kb on - strand at 9.750 kb on - strand at 9.914 kb on + strand, within Pf1N1B4_5588 at 9.915 kb on - strand, within Pf1N1B4_5588 at 10.093 kb on + strand, within Pf1N1B4_5588 at 10.093 kb on + strand, within Pf1N1B4_5588 at 10.094 kb on - strand, within Pf1N1B4_5588 at 10.456 kb on - strand at 10.492 kb on + strand at 10.492 kb on + strand at 10.492 kb on + strand at 10.493 kb on - strand at 10.506 kb on - strand at 10.506 kb on - strand at 11.111 kb on + strand, within Pf1N1B4_5589 at 11.112 kb on - strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.295 kb on + strand, within Pf1N1B4_5589 at 11.314 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.383 kb on + strand, within Pf1N1B4_5589 at 11.541 kb on - strand, within Pf1N1B4_5589 at 11.542 kb on + strand, within Pf1N1B4_5589 at 11.543 kb on - strand, within Pf1N1B4_5589 at 11.543 kb on - strand, within Pf1N1B4_5589 at 11.650 kb on - strand, within Pf1N1B4_5589 at 11.740 kb on + strand at 11.741 kb on - strand at 12.154 kb on - strand, within Pf1N1B4_5590 at 12.310 kb on - strand, within Pf1N1B4_5590 at 12.373 kb on + strand, within Pf1N1B4_5590
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 9,547 + Pf1N1B4_5587 0.80 +1.5 9,548 - Pf1N1B4_5587 0.80 -0.0 9,739 - +0.3 9,750 - -2.5 9,914 + Pf1N1B4_5588 0.23 +0.3 9,915 - Pf1N1B4_5588 0.23 -1.6 10,093 + Pf1N1B4_5588 0.48 +0.7 10,093 + Pf1N1B4_5588 0.48 -0.1 10,094 - Pf1N1B4_5588 0.48 -0.7 10,456 - -1.2 10,492 + +0.7 10,492 + +0.5 10,492 + -2.0 10,493 - +0.4 10,506 - +0.9 10,506 - -0.5 11,111 + Pf1N1B4_5589 0.46 +0.2 11,112 - Pf1N1B4_5589 0.46 +0.1 11,295 + Pf1N1B4_5589 0.60 +1.1 11,295 + Pf1N1B4_5589 0.60 -1.7 11,314 + Pf1N1B4_5589 0.62 -0.8 11,383 + Pf1N1B4_5589 0.67 -0.6 11,383 + Pf1N1B4_5589 0.67 -1.5 11,541 - Pf1N1B4_5589 0.80 -1.0 11,542 + Pf1N1B4_5589 0.80 +2.5 11,543 - Pf1N1B4_5589 0.80 -0.6 11,543 - Pf1N1B4_5589 0.80 -0.5 11,650 - Pf1N1B4_5589 0.89 -2.5 11,740 + +0.1 11,741 - +0.8 12,154 - Pf1N1B4_5590 0.20 -0.3 12,310 - Pf1N1B4_5590 0.28 +0.5 12,373 + Pf1N1B4_5590 0.31 -0.2
Or see this region's nucleotide sequence