Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5289 and Pf1N1B4_5290 are separated by 351 nucleotides Pf1N1B4_5290 and Pf1N1B4_5291 are separated by 290 nucleotides Pf1N1B4_5291 and Pf1N1B4_5292 are separated by 986 nucleotides
Pf1N1B4_5289 - hypothetical protein, at 43,736 to 44,062
_5289
Pf1N1B4_5290 - hypothetical protein, at 44,414 to 44,644
_5290
Pf1N1B4_5291 - Putative reductase, at 44,935 to 46,248
_5291
Pf1N1B4_5292 - hypothetical protein, at 47,235 to 47,396
_5292
Position (kb) 44
45
46
47 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 43.944 kb on + strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 44.127 kb on - strand at 44.255 kb on + strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.275 kb on + strand at 44.415 kb on + strand at 44.650 kb on - strand at 45.101 kb on + strand, within Pf1N1B4_5291 at 45.102 kb on - strand, within Pf1N1B4_5291 at 45.102 kb on - strand, within Pf1N1B4_5291 at 45.102 kb on - strand, within Pf1N1B4_5291 at 45.132 kb on - strand, within Pf1N1B4_5291 at 45.132 kb on - strand, within Pf1N1B4_5291 at 45.132 kb on - strand, within Pf1N1B4_5291 at 45.201 kb on - strand at 45.201 kb on - strand, within Pf1N1B4_5291 at 45.347 kb on + strand, within Pf1N1B4_5291 at 45.347 kb on + strand, within Pf1N1B4_5291 at 45.348 kb on - strand, within Pf1N1B4_5291 at 45.348 kb on - strand, within Pf1N1B4_5291 at 45.348 kb on - strand, within Pf1N1B4_5291 at 45.452 kb on + strand, within Pf1N1B4_5291 at 45.465 kb on - strand, within Pf1N1B4_5291 at 45.500 kb on + strand, within Pf1N1B4_5291 at 45.501 kb on - strand, within Pf1N1B4_5291 at 45.501 kb on - strand, within Pf1N1B4_5291 at 45.501 kb on - strand, within Pf1N1B4_5291 at 45.598 kb on + strand, within Pf1N1B4_5291 at 45.650 kb on + strand, within Pf1N1B4_5291 at 45.651 kb on - strand, within Pf1N1B4_5291 at 45.902 kb on + strand, within Pf1N1B4_5291 at 45.943 kb on + strand at 46.007 kb on + strand, within Pf1N1B4_5291 at 46.231 kb on + strand at 46.256 kb on - strand at 46.256 kb on - strand at 46.325 kb on + strand at 46.326 kb on - strand at 46.389 kb on - strand at 46.421 kb on + strand at 46.422 kb on - strand at 46.532 kb on - strand at 46.542 kb on + strand at 46.574 kb on - strand at 46.574 kb on - strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.786 kb on + strand at 46.787 kb on - strand at 46.787 kb on - strand at 46.787 kb on - strand at 46.844 kb on + strand at 46.844 kb on + strand at 46.845 kb on - strand at 46.927 kb on + strand at 47.002 kb on + strand at 47.002 kb on + strand at 47.002 kb on + strand at 47.018 kb on + strand at 47.019 kb on - strand at 47.032 kb on + strand at 47.032 kb on + strand at 47.032 kb on + strand at 47.033 kb on - strand at 47.066 kb on + strand at 47.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 43,944 + Pf1N1B4_5289 0.64 -1.6 43,945 - Pf1N1B4_5289 0.64 -0.6 43,945 - Pf1N1B4_5289 0.64 -1.0 43,967 - Pf1N1B4_5289 0.71 +0.5 43,967 - Pf1N1B4_5289 0.71 -0.3 44,127 - -0.3 44,255 + +0.7 44,256 - -0.6 44,256 - +1.6 44,256 - +0.0 44,275 + +3.3 44,415 + -1.3 44,650 - -1.8 45,101 + Pf1N1B4_5291 0.13 +0.0 45,102 - Pf1N1B4_5291 0.13 -0.1 45,102 - Pf1N1B4_5291 0.13 +0.0 45,102 - Pf1N1B4_5291 0.13 +2.4 45,132 - Pf1N1B4_5291 0.15 +0.7 45,132 - Pf1N1B4_5291 0.15 +2.6 45,132 - Pf1N1B4_5291 0.15 +0.5 45,201 - -1.0 45,201 - Pf1N1B4_5291 0.20 -1.3 45,347 + Pf1N1B4_5291 0.31 +0.2 45,347 + Pf1N1B4_5291 0.31 -2.1 45,348 - Pf1N1B4_5291 0.31 -0.6 45,348 - Pf1N1B4_5291 0.31 +0.2 45,348 - Pf1N1B4_5291 0.31 +0.1 45,452 + Pf1N1B4_5291 0.39 -0.4 45,465 - Pf1N1B4_5291 0.40 -1.1 45,500 + Pf1N1B4_5291 0.43 +1.0 45,501 - Pf1N1B4_5291 0.43 +1.8 45,501 - Pf1N1B4_5291 0.43 +1.3 45,501 - Pf1N1B4_5291 0.43 +0.0 45,598 + Pf1N1B4_5291 0.50 -0.5 45,650 + Pf1N1B4_5291 0.54 +1.5 45,651 - Pf1N1B4_5291 0.54 +1.6 45,902 + Pf1N1B4_5291 0.74 +0.1 45,943 + -0.6 46,007 + Pf1N1B4_5291 0.82 -0.6 46,231 + +0.6 46,256 - +0.3 46,256 - +0.3 46,325 + -0.0 46,326 - -2.0 46,389 - -1.1 46,421 + -0.9 46,422 - +0.4 46,532 - -0.3 46,542 + -0.6 46,574 - +1.7 46,574 - -0.6 46,786 + +1.0 46,786 + +0.0 46,786 + +1.3 46,786 + +0.3 46,786 + -0.6 46,787 - +0.7 46,787 - -1.3 46,787 - +1.6 46,844 + +0.4 46,844 + +0.4 46,845 - +0.0 46,927 + -1.8 47,002 + +1.5 47,002 + +0.6 47,002 + +0.1 47,018 + -0.9 47,019 - +0.5 47,032 + -0.4 47,032 + -0.1 47,032 + -0.5 47,033 - +0.0 47,066 + -1.1 47,204 + +2.3
Or see this region's nucleotide sequence