Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4995 and Pf1N1B4_4996 are separated by 107 nucleotides Pf1N1B4_4996 and Pf1N1B4_4997 are separated by 40 nucleotides
Pf1N1B4_4995 - Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4), at 5,542,380 to 5,543,303
_4995
Pf1N1B4_4996 - Aspartate aminotransferase (EC 2.6.1.1), at 5,543,411 to 5,544,598
_4996
Pf1N1B4_4997 - Methyl-accepting chemotaxis protein, at 5,544,639 to 5,546,264
_4997
Position (kb) 5543
5544
5545 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5542.502 kb on + strand, within Pf1N1B4_4995 at 5542.503 kb on - strand at 5542.576 kb on - strand, within Pf1N1B4_4995 at 5542.773 kb on + strand, within Pf1N1B4_4995 at 5542.774 kb on - strand, within Pf1N1B4_4995 at 5542.774 kb on - strand at 5542.831 kb on + strand, within Pf1N1B4_4995 at 5542.832 kb on - strand at 5542.832 kb on - strand, within Pf1N1B4_4995 at 5542.832 kb on - strand, within Pf1N1B4_4995 at 5542.836 kb on - strand at 5542.906 kb on - strand, within Pf1N1B4_4995 at 5543.089 kb on - strand, within Pf1N1B4_4995 at 5543.235 kb on + strand at 5543.235 kb on + strand at 5543.245 kb on - strand at 5543.289 kb on + strand at 5543.510 kb on - strand at 5543.759 kb on + strand, within Pf1N1B4_4996 at 5543.979 kb on + strand, within Pf1N1B4_4996 at 5544.213 kb on + strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.214 kb on - strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.222 kb on + strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.223 kb on - strand, within Pf1N1B4_4996 at 5544.283 kb on - strand, within Pf1N1B4_4996 at 5544.370 kb on + strand, within Pf1N1B4_4996 at 5544.371 kb on - strand, within Pf1N1B4_4996 at 5544.371 kb on - strand at 5544.396 kb on - strand, within Pf1N1B4_4996 at 5544.600 kb on + strand at 5544.600 kb on + strand at 5544.600 kb on + strand at 5544.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 5,542,502 + Pf1N1B4_4995 0.13 +0.9 5,542,503 - -1.6 5,542,576 - Pf1N1B4_4995 0.21 -0.2 5,542,773 + Pf1N1B4_4995 0.43 +1.4 5,542,774 - Pf1N1B4_4995 0.43 -0.2 5,542,774 - -0.6 5,542,831 + Pf1N1B4_4995 0.49 +1.8 5,542,832 - +0.7 5,542,832 - Pf1N1B4_4995 0.49 -0.1 5,542,832 - Pf1N1B4_4995 0.49 +1.4 5,542,836 - -2.2 5,542,906 - Pf1N1B4_4995 0.57 +1.5 5,543,089 - Pf1N1B4_4995 0.77 -0.3 5,543,235 + -0.6 5,543,235 + +0.4 5,543,245 - +0.9 5,543,289 + +0.6 5,543,510 - -1.8 5,543,759 + Pf1N1B4_4996 0.29 -2.6 5,543,979 + Pf1N1B4_4996 0.48 -0.3 5,544,213 + Pf1N1B4_4996 0.68 -0.4 5,544,214 - Pf1N1B4_4996 0.68 -0.1 5,544,214 - Pf1N1B4_4996 0.68 +0.9 5,544,214 - Pf1N1B4_4996 0.68 -1.2 5,544,214 - Pf1N1B4_4996 0.68 -0.5 5,544,222 + Pf1N1B4_4996 0.68 -0.3 5,544,222 + Pf1N1B4_4996 0.68 -0.6 5,544,222 + Pf1N1B4_4996 0.68 +0.4 5,544,222 + Pf1N1B4_4996 0.68 +0.4 5,544,222 + Pf1N1B4_4996 0.68 +0.8 5,544,223 - Pf1N1B4_4996 0.68 -0.6 5,544,223 - Pf1N1B4_4996 0.68 -0.2 5,544,223 - Pf1N1B4_4996 0.68 +0.9 5,544,223 - Pf1N1B4_4996 0.68 -1.6 5,544,283 - Pf1N1B4_4996 0.73 -0.0 5,544,370 + Pf1N1B4_4996 0.81 -0.1 5,544,371 - Pf1N1B4_4996 0.81 -0.2 5,544,371 - +0.9 5,544,396 - Pf1N1B4_4996 0.83 +0.7 5,544,600 + -0.4 5,544,600 + -0.1 5,544,600 + +2.2 5,544,636 - +2.5
Or see this region's nucleotide sequence