Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4821 and Pf1N1B4_4822 overlap by 20 nucleotides Pf1N1B4_4822 and Pf1N1B4_4823 overlap by 4 nucleotides Pf1N1B4_4823 and Pf1N1B4_4824 are separated by 288 nucleotides
Pf1N1B4_4821 - Paraquat-inducible protein B, at 5,324,745 to 5,326,400
_4821
Pf1N1B4_4822 - Paraquat-inducible protein A, at 5,326,381 to 5,327,061
_4822
Pf1N1B4_4823 - Paraquat-inducible protein A, at 5,327,058 to 5,327,654
_4823
Pf1N1B4_4824 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 5,327,943 to 5,329,244
_4824
Position (kb)
5327
5328 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5326.272 kb on + strand at 5326.273 kb on - strand at 5326.498 kb on + strand, within Pf1N1B4_4822 at 5326.499 kb on - strand, within Pf1N1B4_4822 at 5326.499 kb on - strand, within Pf1N1B4_4822 at 5326.761 kb on + strand, within Pf1N1B4_4822 at 5326.883 kb on + strand, within Pf1N1B4_4822 at 5326.883 kb on + strand at 5326.884 kb on - strand, within Pf1N1B4_4822 at 5326.908 kb on - strand, within Pf1N1B4_4822 at 5327.061 kb on + strand at 5327.082 kb on + strand at 5327.083 kb on - strand at 5327.151 kb on + strand, within Pf1N1B4_4823 at 5327.154 kb on + strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.369 kb on + strand, within Pf1N1B4_4823 at 5327.461 kb on + strand, within Pf1N1B4_4823 at 5327.564 kb on + strand, within Pf1N1B4_4823 at 5327.564 kb on + strand, within Pf1N1B4_4823 at 5327.565 kb on - strand, within Pf1N1B4_4823 at 5327.605 kb on + strand at 5327.649 kb on - strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.703 kb on - strand at 5327.728 kb on + strand at 5327.729 kb on - strand at 5327.729 kb on - strand at 5327.756 kb on + strand at 5327.756 kb on + strand at 5327.757 kb on - strand at 5327.887 kb on + strand at 5327.888 kb on - strand at 5327.888 kb on - strand at 5328.229 kb on + strand at 5328.284 kb on + strand, within Pf1N1B4_4824 at 5328.285 kb on - strand, within Pf1N1B4_4824 at 5328.287 kb on - strand, within Pf1N1B4_4824 at 5328.319 kb on + strand, within Pf1N1B4_4824 at 5328.464 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 5,326,272 + -2.0 5,326,273 - -0.4 5,326,498 + Pf1N1B4_4822 0.17 +0.6 5,326,499 - Pf1N1B4_4822 0.17 -2.7 5,326,499 - Pf1N1B4_4822 0.17 +1.5 5,326,761 + Pf1N1B4_4822 0.56 +3.6 5,326,883 + Pf1N1B4_4822 0.74 -0.3 5,326,883 + +0.3 5,326,884 - Pf1N1B4_4822 0.74 -0.3 5,326,908 - Pf1N1B4_4822 0.77 +1.4 5,327,061 + -1.7 5,327,082 + -0.6 5,327,083 - +1.5 5,327,151 + Pf1N1B4_4823 0.16 +0.3 5,327,154 + Pf1N1B4_4823 0.16 -1.3 5,327,155 - Pf1N1B4_4823 0.16 -2.3 5,327,155 - Pf1N1B4_4823 0.16 +0.9 5,327,155 - Pf1N1B4_4823 0.16 +0.3 5,327,155 - Pf1N1B4_4823 0.16 -1.9 5,327,369 + Pf1N1B4_4823 0.52 +0.8 5,327,461 + Pf1N1B4_4823 0.68 -2.4 5,327,564 + Pf1N1B4_4823 0.85 -0.7 5,327,564 + Pf1N1B4_4823 0.85 -1.0 5,327,565 - Pf1N1B4_4823 0.85 -1.0 5,327,605 + +0.8 5,327,649 - +0.7 5,327,702 + -0.7 5,327,702 + +1.3 5,327,702 + -0.7 5,327,702 + -1.1 5,327,702 + -2.4 5,327,702 + -0.3 5,327,703 - -1.2 5,327,728 + +0.3 5,327,729 - -0.7 5,327,729 - -0.3 5,327,756 + -1.1 5,327,756 + -0.1 5,327,757 - -3.0 5,327,887 + +0.9 5,327,888 - +1.1 5,327,888 - +0.1 5,328,229 + +1.3 5,328,284 + Pf1N1B4_4824 0.26 +0.8 5,328,285 - Pf1N1B4_4824 0.26 +0.3 5,328,287 - Pf1N1B4_4824 0.26 -2.5 5,328,319 + Pf1N1B4_4824 0.29 +0.3 5,328,464 - +0.3
Or see this region's nucleotide sequence