Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4690 and Pf1N1B4_4691 overlap by 1 nucleotides Pf1N1B4_4691 and Pf1N1B4_4692 are separated by 454 nucleotides Pf1N1B4_4692 and Pf1N1B4_4693 are separated by 49 nucleotides
Pf1N1B4_4690 - probable membrane protein YPO3302, at 5,182,766 to 5,183,200
_4690
Pf1N1B4_4691 - CopG protein, at 5,183,200 to 5,183,646
_4691
Pf1N1B4_4692 - hypothetical protein, at 5,184,101 to 5,184,631
_4692
Pf1N1B4_4693 - Oxidoreductase, at 5,184,681 to 5,185,676
_4693
Position (kb) 5184
5185 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5183.188 kb on - strand at 5183.264 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.363 kb on - strand, within Pf1N1B4_4691 at 5183.406 kb on + strand, within Pf1N1B4_4691 at 5183.483 kb on + strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.696 kb on - strand at 5183.728 kb on + strand at 5183.728 kb on + strand at 5183.837 kb on + strand at 5183.921 kb on + strand at 5183.961 kb on - strand at 5184.005 kb on + strand at 5184.005 kb on + strand at 5184.116 kb on - strand at 5184.455 kb on + strand at 5184.493 kb on + strand, within Pf1N1B4_4692 at 5184.493 kb on + strand, within Pf1N1B4_4692 at 5184.494 kb on - strand, within Pf1N1B4_4692 at 5184.494 kb on - strand, within Pf1N1B4_4692 at 5184.533 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand at 5184.548 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand, within Pf1N1B4_4692 at 5184.548 kb on + strand at 5184.548 kb on + strand at 5184.552 kb on + strand, within Pf1N1B4_4692 at 5184.552 kb on + strand, within Pf1N1B4_4692 at 5184.572 kb on + strand, within Pf1N1B4_4692 at 5184.818 kb on - strand, within Pf1N1B4_4693 at 5184.818 kb on - strand, within Pf1N1B4_4693 at 5184.821 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand, within Pf1N1B4_4693 at 5185.365 kb on + strand at 5185.365 kb on + strand at 5185.366 kb on - strand, within Pf1N1B4_4693 at 5185.366 kb on - strand, within Pf1N1B4_4693 at 5185.366 kb on - strand at 5185.366 kb on - strand, within Pf1N1B4_4693 at 5185.369 kb on + strand, within Pf1N1B4_4693 at 5185.370 kb on - strand, within Pf1N1B4_4693 at 5185.370 kb on - strand, within Pf1N1B4_4693 at 5185.496 kb on + strand, within Pf1N1B4_4693 at 5185.497 kb on - strand, within Pf1N1B4_4693 at 5185.497 kb on - strand, within Pf1N1B4_4693 at 5185.508 kb on - strand, within Pf1N1B4_4693 at 5185.508 kb on - strand, within Pf1N1B4_4693 at 5185.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 5,183,188 - +0.9 5,183,264 + Pf1N1B4_4691 0.14 +0.1 5,183,362 + Pf1N1B4_4691 0.36 -0.8 5,183,362 + Pf1N1B4_4691 0.36 +2.4 5,183,363 - Pf1N1B4_4691 0.36 -0.1 5,183,406 + Pf1N1B4_4691 0.46 -0.6 5,183,483 + Pf1N1B4_4691 0.63 -0.5 5,183,484 - Pf1N1B4_4691 0.64 -0.4 5,183,484 - Pf1N1B4_4691 0.64 +0.8 5,183,484 - Pf1N1B4_4691 0.64 -0.4 5,183,696 - -1.5 5,183,728 + +0.0 5,183,728 + -1.8 5,183,837 + -0.6 5,183,921 + +1.1 5,183,961 - -2.0 5,184,005 + -0.4 5,184,005 + -1.7 5,184,116 - -1.2 5,184,455 + -1.2 5,184,493 + Pf1N1B4_4692 0.74 +0.3 5,184,493 + Pf1N1B4_4692 0.74 -2.2 5,184,494 - Pf1N1B4_4692 0.74 -1.4 5,184,494 - Pf1N1B4_4692 0.74 -0.2 5,184,533 + Pf1N1B4_4692 0.81 +1.2 5,184,548 + Pf1N1B4_4692 0.84 -1.7 5,184,548 + -0.2 5,184,548 + Pf1N1B4_4692 0.84 -0.9 5,184,548 + Pf1N1B4_4692 0.84 -0.2 5,184,548 + -0.7 5,184,548 + +0.9 5,184,552 + Pf1N1B4_4692 0.85 +0.7 5,184,552 + Pf1N1B4_4692 0.85 +0.3 5,184,572 + Pf1N1B4_4692 0.89 +0.9 5,184,818 - Pf1N1B4_4693 0.14 -0.0 5,184,818 - Pf1N1B4_4693 0.14 -1.0 5,184,821 + Pf1N1B4_4693 0.14 -1.2 5,185,365 + Pf1N1B4_4693 0.69 -0.7 5,185,365 + Pf1N1B4_4693 0.69 -1.7 5,185,365 + Pf1N1B4_4693 0.69 +1.3 5,185,365 + +2.7 5,185,365 + +0.3 5,185,366 - Pf1N1B4_4693 0.69 -1.4 5,185,366 - Pf1N1B4_4693 0.69 +0.2 5,185,366 - -0.7 5,185,366 - Pf1N1B4_4693 0.69 -0.1 5,185,369 + Pf1N1B4_4693 0.69 -0.7 5,185,370 - Pf1N1B4_4693 0.69 +0.7 5,185,370 - Pf1N1B4_4693 0.69 -0.1 5,185,496 + Pf1N1B4_4693 0.82 -1.7 5,185,497 - Pf1N1B4_4693 0.82 -0.2 5,185,497 - Pf1N1B4_4693 0.82 +0.3 5,185,508 - Pf1N1B4_4693 0.83 +1.2 5,185,508 - Pf1N1B4_4693 0.83 -1.1 5,185,598 - -0.7
Or see this region's nucleotide sequence