Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4689 and Pf1N1B4_4690 are separated by 221 nucleotides Pf1N1B4_4690 and Pf1N1B4_4691 overlap by 1 nucleotides Pf1N1B4_4691 and Pf1N1B4_4692 are separated by 454 nucleotides
Pf1N1B4_4689 - hydrolase, alpha/beta fold family, at 5,181,501 to 5,182,544
_4689
Pf1N1B4_4690 - probable membrane protein YPO3302, at 5,182,766 to 5,183,200
_4690
Pf1N1B4_4691 - CopG protein, at 5,183,200 to 5,183,646
_4691
Pf1N1B4_4692 - hypothetical protein, at 5,184,101 to 5,184,631
_4692
Position (kb) 5182
5183
5184 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5181.766 kb on + strand, within Pf1N1B4_4689 at 5181.767 kb on - strand, within Pf1N1B4_4689 at 5181.854 kb on - strand at 5181.898 kb on + strand, within Pf1N1B4_4689 at 5181.899 kb on - strand, within Pf1N1B4_4689 at 5181.899 kb on - strand, within Pf1N1B4_4689 at 5181.899 kb on - strand, within Pf1N1B4_4689 at 5181.901 kb on + strand, within Pf1N1B4_4689 at 5181.901 kb on + strand at 5181.901 kb on + strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.902 kb on - strand, within Pf1N1B4_4689 at 5181.958 kb on + strand, within Pf1N1B4_4689 at 5181.980 kb on - strand, within Pf1N1B4_4689 at 5182.055 kb on - strand, within Pf1N1B4_4689 at 5182.174 kb on + strand, within Pf1N1B4_4689 at 5182.354 kb on + strand, within Pf1N1B4_4689 at 5182.396 kb on + strand, within Pf1N1B4_4689 at 5182.397 kb on - strand, within Pf1N1B4_4689 at 5182.397 kb on - strand at 5182.397 kb on - strand, within Pf1N1B4_4689 at 5182.408 kb on + strand, within Pf1N1B4_4689 at 5182.601 kb on + strand at 5182.607 kb on + strand at 5182.607 kb on + strand at 5182.607 kb on + strand at 5182.608 kb on - strand at 5182.786 kb on + strand at 5182.917 kb on - strand, within Pf1N1B4_4690 at 5183.188 kb on - strand at 5183.264 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.362 kb on + strand, within Pf1N1B4_4691 at 5183.363 kb on - strand, within Pf1N1B4_4691 at 5183.406 kb on + strand, within Pf1N1B4_4691 at 5183.483 kb on + strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.484 kb on - strand, within Pf1N1B4_4691 at 5183.696 kb on - strand at 5183.728 kb on + strand at 5183.728 kb on + strand at 5183.837 kb on + strand at 5183.921 kb on + strand at 5183.961 kb on - strand at 5184.005 kb on + strand at 5184.005 kb on + strand at 5184.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 5,181,766 + Pf1N1B4_4689 0.25 +1.2 5,181,767 - Pf1N1B4_4689 0.25 -0.3 5,181,854 - +0.4 5,181,898 + Pf1N1B4_4689 0.38 -2.0 5,181,899 - Pf1N1B4_4689 0.38 -2.4 5,181,899 - Pf1N1B4_4689 0.38 -1.2 5,181,899 - Pf1N1B4_4689 0.38 -1.1 5,181,901 + Pf1N1B4_4689 0.38 +0.8 5,181,901 + +0.0 5,181,901 + Pf1N1B4_4689 0.38 -2.6 5,181,902 - Pf1N1B4_4689 0.38 -0.4 5,181,902 - Pf1N1B4_4689 0.38 -2.0 5,181,902 - -0.6 5,181,902 - Pf1N1B4_4689 0.38 +0.4 5,181,902 - Pf1N1B4_4689 0.38 -0.6 5,181,902 - Pf1N1B4_4689 0.38 -0.1 5,181,902 - Pf1N1B4_4689 0.38 +0.4 5,181,958 + Pf1N1B4_4689 0.44 -0.2 5,181,980 - Pf1N1B4_4689 0.46 -0.3 5,182,055 - Pf1N1B4_4689 0.53 +0.9 5,182,174 + Pf1N1B4_4689 0.64 -1.2 5,182,354 + Pf1N1B4_4689 0.82 -1.6 5,182,396 + Pf1N1B4_4689 0.86 +0.7 5,182,397 - Pf1N1B4_4689 0.86 -0.0 5,182,397 - -1.6 5,182,397 - Pf1N1B4_4689 0.86 +0.8 5,182,408 + Pf1N1B4_4689 0.87 +1.7 5,182,601 + +0.7 5,182,607 + +0.9 5,182,607 + -1.2 5,182,607 + +2.7 5,182,608 - +0.4 5,182,786 + -0.8 5,182,917 - Pf1N1B4_4690 0.35 -0.6 5,183,188 - +0.9 5,183,264 + Pf1N1B4_4691 0.14 +0.1 5,183,362 + Pf1N1B4_4691 0.36 -0.8 5,183,362 + Pf1N1B4_4691 0.36 +2.4 5,183,363 - Pf1N1B4_4691 0.36 -0.1 5,183,406 + Pf1N1B4_4691 0.46 -0.6 5,183,483 + Pf1N1B4_4691 0.63 -0.5 5,183,484 - Pf1N1B4_4691 0.64 -0.4 5,183,484 - Pf1N1B4_4691 0.64 +0.8 5,183,484 - Pf1N1B4_4691 0.64 -0.4 5,183,696 - -1.5 5,183,728 + +0.0 5,183,728 + -1.8 5,183,837 + -0.6 5,183,921 + +1.1 5,183,961 - -2.0 5,184,005 + -0.4 5,184,005 + -1.7 5,184,116 - -1.2
Or see this region's nucleotide sequence