Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4440 and Pf1N1B4_4441 are separated by 176 nucleotides Pf1N1B4_4441 and Pf1N1B4_4442 are separated by 117 nucleotides Pf1N1B4_4442 and Pf1N1B4_4443 overlap by 15 nucleotides Pf1N1B4_4443 and Pf1N1B4_4444 are separated by 210 nucleotides
Pf1N1B4_4440 - Carbonic anhydrase (EC 4.2.1.1), at 4,900,532 to 4,901,191
_4440
Pf1N1B4_4441 - Cyanate hydratase (EC 4.2.1.104), at 4,901,368 to 4,901,835
_4441
Pf1N1B4_4442 - Cyn operon transcriptional activator, at 4,901,953 to 4,902,858
_4442
Pf1N1B4_4443 - hypothetical protein, at 4,902,844 to 4,903,059
_4443
Pf1N1B4_4444 - FIG00960995: hypothetical protein, at 4,903,270 to 4,903,617
_4444
Position (kb)
4901
4902
4903 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4900.984 kb on - strand, within Pf1N1B4_4440 at 4901.134 kb on + strand at 4901.236 kb on + strand at 4901.528 kb on + strand, within Pf1N1B4_4441 at 4901.555 kb on + strand, within Pf1N1B4_4441 at 4901.711 kb on + strand, within Pf1N1B4_4441 at 4901.711 kb on + strand, within Pf1N1B4_4441 at 4901.816 kb on + strand at 4901.816 kb on + strand at 4901.913 kb on - strand at 4901.913 kb on - strand at 4901.913 kb on - strand at 4901.913 kb on - strand at 4901.918 kb on - strand at 4901.918 kb on - strand at 4901.918 kb on - strand at 4901.918 kb on - strand at 4901.926 kb on + strand at 4901.937 kb on + strand at 4901.939 kb on + strand at 4901.939 kb on + strand at 4901.939 kb on + strand at 4901.940 kb on - strand at 4901.940 kb on - strand at 4901.975 kb on - strand at 4902.142 kb on + strand, within Pf1N1B4_4442 at 4902.142 kb on + strand, within Pf1N1B4_4442 at 4902.142 kb on + strand, within Pf1N1B4_4442 at 4902.142 kb on + strand at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand, within Pf1N1B4_4442 at 4902.143 kb on - strand at 4902.145 kb on - strand, within Pf1N1B4_4442 at 4902.262 kb on + strand, within Pf1N1B4_4442 at 4902.263 kb on - strand, within Pf1N1B4_4442 at 4902.289 kb on + strand at 4902.289 kb on + strand, within Pf1N1B4_4442 at 4902.478 kb on + strand, within Pf1N1B4_4442 at 4902.574 kb on + strand, within Pf1N1B4_4442 at 4902.775 kb on + strand at 4902.775 kb on + strand at 4902.776 kb on - strand at 4902.776 kb on - strand at 4902.776 kb on - strand at 4903.184 kb on + strand at 4903.184 kb on + strand at 4903.184 kb on + strand at 4903.184 kb on + strand at 4903.185 kb on - strand at 4903.190 kb on + strand at 4903.190 kb on + strand at 4903.191 kb on - strand at 4903.256 kb on - strand at 4903.269 kb on - strand at 4903.275 kb on - strand at 4903.275 kb on - strand at 4903.275 kb on - strand at 4903.275 kb on - strand at 4903.391 kb on + strand, within Pf1N1B4_4444 at 4903.392 kb on - strand, within Pf1N1B4_4444 at 4903.522 kb on - strand, within Pf1N1B4_4444 at 4903.602 kb on + strand at 4903.603 kb on - strand at 4903.647 kb on + strand at 4903.648 kb on - strand at 4903.665 kb on + strand at 4903.742 kb on - strand at 4903.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 4,900,984 - Pf1N1B4_4440 0.68 -0.5 4,901,134 + -0.3 4,901,236 + +0.2 4,901,528 + Pf1N1B4_4441 0.34 -1.1 4,901,555 + Pf1N1B4_4441 0.40 -0.0 4,901,711 + Pf1N1B4_4441 0.73 -0.7 4,901,711 + Pf1N1B4_4441 0.73 +0.3 4,901,816 + +1.5 4,901,816 + +0.8 4,901,913 - -1.5 4,901,913 - -0.5 4,901,913 - +0.9 4,901,913 - +0.8 4,901,918 - +0.4 4,901,918 - -1.2 4,901,918 - -0.5 4,901,918 - +0.9 4,901,926 + -0.1 4,901,937 + -0.5 4,901,939 + +1.7 4,901,939 + -0.5 4,901,939 + +0.3 4,901,940 - -2.1 4,901,940 - +0.1 4,901,975 - -1.5 4,902,142 + Pf1N1B4_4442 0.21 +0.1 4,902,142 + Pf1N1B4_4442 0.21 -2.7 4,902,142 + Pf1N1B4_4442 0.21 -0.5 4,902,142 + +1.5 4,902,143 - Pf1N1B4_4442 0.21 -0.0 4,902,143 - Pf1N1B4_4442 0.21 -2.1 4,902,143 - Pf1N1B4_4442 0.21 +1.9 4,902,143 - Pf1N1B4_4442 0.21 +0.1 4,902,143 - Pf1N1B4_4442 0.21 +0.6 4,902,143 - +0.9 4,902,145 - Pf1N1B4_4442 0.21 -0.6 4,902,262 + Pf1N1B4_4442 0.34 -0.6 4,902,263 - Pf1N1B4_4442 0.34 -0.5 4,902,289 + -0.5 4,902,289 + Pf1N1B4_4442 0.37 +1.3 4,902,478 + Pf1N1B4_4442 0.58 -1.5 4,902,574 + Pf1N1B4_4442 0.69 -1.1 4,902,775 + +1.5 4,902,775 + +0.5 4,902,776 - +0.2 4,902,776 - +0.1 4,902,776 - -0.5 4,903,184 + +0.2 4,903,184 + +0.1 4,903,184 + -1.5 4,903,184 + +1.0 4,903,185 - -0.4 4,903,190 + +0.2 4,903,190 + +0.5 4,903,191 - -1.1 4,903,256 - +0.8 4,903,269 - +1.8 4,903,275 - +1.5 4,903,275 - -0.0 4,903,275 - -2.2 4,903,275 - +1.2 4,903,391 + Pf1N1B4_4444 0.35 -0.5 4,903,392 - Pf1N1B4_4444 0.35 -0.5 4,903,522 - Pf1N1B4_4444 0.72 +1.0 4,903,602 + +1.5 4,903,603 - -1.5 4,903,647 + +0.0 4,903,648 - -0.5 4,903,665 + -0.5 4,903,742 - +0.1 4,903,742 - -1.1
Or see this region's nucleotide sequence