Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3887 and Pf1N1B4_3888 are separated by 236 nucleotides Pf1N1B4_3888 and Pf1N1B4_3889 are separated by 60 nucleotides Pf1N1B4_3889 and Pf1N1B4_3890 overlap by 21 nucleotides
Pf1N1B4_3887 - CAAX amino terminal protease family protein, at 4,315,990 to 4,316,781
_3887
Pf1N1B4_3888 - Aconitate hydratase (EC 4.2.1.3), at 4,317,018 to 4,319,759
_3888
Pf1N1B4_3889 - hypothetical protein, at 4,319,820 to 4,320,002
_3889
Pf1N1B4_3890 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 4,319,982 to 4,321,055
_3890
Position (kb)
4317
4318
4319
4320 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4316.183 kb on - strand, within Pf1N1B4_3887 at 4316.243 kb on + strand, within Pf1N1B4_3887 at 4316.243 kb on + strand, within Pf1N1B4_3887 at 4316.498 kb on + strand, within Pf1N1B4_3887 at 4316.499 kb on - strand, within Pf1N1B4_3887 at 4316.527 kb on - strand, within Pf1N1B4_3887 at 4316.625 kb on - strand at 4316.717 kb on + strand at 4316.717 kb on + strand at 4316.759 kb on + strand at 4316.759 kb on + strand at 4316.760 kb on - strand at 4316.760 kb on - strand at 4316.955 kb on - strand at 4316.955 kb on - strand at 4316.956 kb on + strand at 4316.956 kb on + strand at 4316.956 kb on + strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.957 kb on - strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.964 kb on + strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.965 kb on - strand at 4316.967 kb on - strand at 4316.967 kb on - strand at 4316.974 kb on - strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.046 kb on + strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.047 kb on - strand at 4317.073 kb on + strand at 4317.073 kb on + strand at 4317.074 kb on - strand at 4317.074 kb on - strand at 4317.074 kb on - strand at 4317.270 kb on + strand at 4317.270 kb on + strand at 4317.270 kb on + strand at 4317.270 kb on + strand at 4317.271 kb on - strand at 4317.388 kb on + strand, within Pf1N1B4_3888 at 4317.389 kb on - strand, within Pf1N1B4_3888 at 4317.451 kb on + strand, within Pf1N1B4_3888 at 4317.451 kb on + strand, within Pf1N1B4_3888 at 4317.452 kb on - strand, within Pf1N1B4_3888 at 4317.479 kb on + strand, within Pf1N1B4_3888 at 4317.479 kb on + strand, within Pf1N1B4_3888 at 4317.479 kb on + strand at 4317.479 kb on + strand, within Pf1N1B4_3888 at 4317.481 kb on + strand, within Pf1N1B4_3888 at 4317.482 kb on - strand at 4317.482 kb on - strand at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.482 kb on - strand, within Pf1N1B4_3888 at 4317.786 kb on - strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.787 kb on + strand, within Pf1N1B4_3888 at 4317.788 kb on - strand at 4317.788 kb on - strand at 4317.788 kb on - strand, within Pf1N1B4_3888 at 4317.788 kb on - strand at 4317.788 kb on - strand, within Pf1N1B4_3888 at 4317.788 kb on - strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.811 kb on + strand, within Pf1N1B4_3888 at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.812 kb on - strand at 4317.812 kb on - strand, within Pf1N1B4_3888 at 4317.945 kb on - strand, within Pf1N1B4_3888 at 4317.945 kb on - strand, within Pf1N1B4_3888 at 4317.945 kb on - strand, within Pf1N1B4_3888 at 4317.956 kb on - strand, within Pf1N1B4_3888 at 4318.240 kb on + strand, within Pf1N1B4_3888 at 4318.241 kb on - strand, within Pf1N1B4_3888 at 4318.264 kb on + strand, within Pf1N1B4_3888 at 4318.264 kb on + strand, within Pf1N1B4_3888 at 4318.264 kb on + strand, within Pf1N1B4_3888 at 4318.264 kb on + strand at 4318.267 kb on + strand, within Pf1N1B4_3888 at 4318.267 kb on + strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.268 kb on - strand, within Pf1N1B4_3888 at 4318.444 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.448 kb on + strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.449 kb on - strand, within Pf1N1B4_3888 at 4318.730 kb on + strand, within Pf1N1B4_3888 at 4318.730 kb on + strand, within Pf1N1B4_3888 at 4318.730 kb on + strand at 4318.731 kb on - strand, within Pf1N1B4_3888 at 4318.731 kb on - strand, within Pf1N1B4_3888 at 4318.732 kb on + strand, within Pf1N1B4_3888 at 4318.733 kb on - strand, within Pf1N1B4_3888 at 4318.733 kb on - strand at 4318.733 kb on - strand, within Pf1N1B4_3888 at 4318.784 kb on - strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.155 kb on + strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.156 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.201 kb on - strand, within Pf1N1B4_3888 at 4319.259 kb on - strand, within Pf1N1B4_3888 at 4319.353 kb on + strand at 4319.353 kb on + strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.354 kb on - strand, within Pf1N1B4_3888 at 4319.502 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.701 kb on + strand at 4319.702 kb on - strand at 4319.721 kb on + strand at 4319.722 kb on - strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.774 kb on + strand at 4319.775 kb on - strand at 4319.816 kb on - strand at 4319.816 kb on - strand at 4319.831 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.833 kb on + strand at 4319.834 kb on - strand at 4319.847 kb on + strand, within Pf1N1B4_3889 at 4319.859 kb on - strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.977 kb on + strand, within Pf1N1B4_3889 at 4319.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 4,316,183 - Pf1N1B4_3887 0.24 +2.2 4,316,243 + Pf1N1B4_3887 0.32 +0.2 4,316,243 + Pf1N1B4_3887 0.32 -1.5 4,316,498 + Pf1N1B4_3887 0.64 -0.8 4,316,499 - Pf1N1B4_3887 0.64 -1.0 4,316,527 - Pf1N1B4_3887 0.68 +0.5 4,316,625 - +0.2 4,316,717 + -0.2 4,316,717 + -0.6 4,316,759 + -0.3 4,316,759 + +0.8 4,316,760 - +0.1 4,316,760 - -0.8 4,316,955 - +0.7 4,316,955 - +0.3 4,316,956 + +3.6 4,316,956 + +0.1 4,316,956 + -2.2 4,316,957 - +0.3 4,316,957 - +0.2 4,316,957 - -0.5 4,316,957 - -0.4 4,316,957 - +0.3 4,316,957 - +1.5 4,316,957 - +0.6 4,316,957 - +1.5 4,316,957 - +1.0 4,316,957 - +0.8 4,316,964 + -0.5 4,316,964 + -0.8 4,316,964 + +1.2 4,316,964 + +1.4 4,316,964 + +1.4 4,316,964 + +1.5 4,316,964 + -0.8 4,316,964 + -0.8 4,316,965 - +0.8 4,316,965 - -0.6 4,316,965 - +0.9 4,316,965 - +0.1 4,316,965 - +0.3 4,316,965 - -0.6 4,316,965 - +0.4 4,316,965 - -1.8 4,316,965 - +1.4 4,316,965 - +0.9 4,316,965 - +0.4 4,316,965 - -1.4 4,316,965 - +0.5 4,316,965 - -0.5 4,316,965 - +0.4 4,316,965 - +0.3 4,316,967 - +0.3 4,316,967 - +1.4 4,316,974 - +0.9 4,317,046 + -0.2 4,317,046 + -1.2 4,317,046 + +0.6 4,317,046 + +0.2 4,317,046 + +0.2 4,317,047 - +0.0 4,317,047 - -0.1 4,317,047 - -1.4 4,317,047 - -0.3 4,317,047 - -1.1 4,317,047 - +0.0 4,317,047 - +0.8 4,317,047 - +0.8 4,317,073 + -2.0 4,317,073 + -0.2 4,317,074 - -0.2 4,317,074 - -1.2 4,317,074 - +0.8 4,317,270 + +1.4 4,317,270 + +0.9 4,317,270 + +0.2 4,317,270 + +0.5 4,317,271 - -0.2 4,317,388 + Pf1N1B4_3888 0.13 -2.3 4,317,389 - Pf1N1B4_3888 0.14 +0.4 4,317,451 + Pf1N1B4_3888 0.16 -1.5 4,317,451 + Pf1N1B4_3888 0.16 +0.4 4,317,452 - Pf1N1B4_3888 0.16 -2.4 4,317,479 + Pf1N1B4_3888 0.17 -1.3 4,317,479 + Pf1N1B4_3888 0.17 +1.1 4,317,479 + -0.2 4,317,479 + Pf1N1B4_3888 0.17 +1.0 4,317,481 + Pf1N1B4_3888 0.17 +1.1 4,317,482 - -0.2 4,317,482 - +2.4 4,317,482 - Pf1N1B4_3888 0.17 +1.3 4,317,482 - Pf1N1B4_3888 0.17 +0.4 4,317,482 - Pf1N1B4_3888 0.17 +1.7 4,317,482 - Pf1N1B4_3888 0.17 -1.1 4,317,482 - Pf1N1B4_3888 0.17 +0.8 4,317,786 - Pf1N1B4_3888 0.28 +0.8 4,317,787 + Pf1N1B4_3888 0.28 -1.0 4,317,787 + Pf1N1B4_3888 0.28 +0.0 4,317,787 + Pf1N1B4_3888 0.28 +1.3 4,317,787 + +2.4 4,317,787 + Pf1N1B4_3888 0.28 +0.5 4,317,787 + Pf1N1B4_3888 0.28 +0.5 4,317,787 + Pf1N1B4_3888 0.28 -1.8 4,317,787 + Pf1N1B4_3888 0.28 -1.5 4,317,787 + Pf1N1B4_3888 0.28 -1.2 4,317,788 - -0.2 4,317,788 - +0.5 4,317,788 - Pf1N1B4_3888 0.28 +1.1 4,317,788 - +0.8 4,317,788 - Pf1N1B4_3888 0.28 +0.8 4,317,788 - Pf1N1B4_3888 0.28 +0.8 4,317,811 + Pf1N1B4_3888 0.29 +0.2 4,317,811 + Pf1N1B4_3888 0.29 +0.5 4,317,811 + Pf1N1B4_3888 0.29 -1.4 4,317,811 + Pf1N1B4_3888 0.29 -1.0 4,317,812 - Pf1N1B4_3888 0.29 +0.0 4,317,812 - Pf1N1B4_3888 0.29 +1.2 4,317,812 - Pf1N1B4_3888 0.29 -0.0 4,317,812 - -0.2 4,317,812 - Pf1N1B4_3888 0.29 +0.8 4,317,945 - Pf1N1B4_3888 0.34 +0.8 4,317,945 - Pf1N1B4_3888 0.34 +2.8 4,317,945 - Pf1N1B4_3888 0.34 -1.5 4,317,956 - Pf1N1B4_3888 0.34 -0.0 4,318,240 + Pf1N1B4_3888 0.45 +1.6 4,318,241 - Pf1N1B4_3888 0.45 +0.8 4,318,264 + Pf1N1B4_3888 0.45 -0.1 4,318,264 + Pf1N1B4_3888 0.45 +0.8 4,318,264 + Pf1N1B4_3888 0.45 -2.0 4,318,264 + -0.2 4,318,267 + Pf1N1B4_3888 0.46 +0.8 4,318,267 + Pf1N1B4_3888 0.46 -1.2 4,318,268 - Pf1N1B4_3888 0.46 -1.4 4,318,268 - Pf1N1B4_3888 0.46 +0.0 4,318,268 - Pf1N1B4_3888 0.46 -0.8 4,318,268 - Pf1N1B4_3888 0.46 -1.8 4,318,444 + Pf1N1B4_3888 0.52 +0.1 4,318,448 + Pf1N1B4_3888 0.52 +0.1 4,318,448 + Pf1N1B4_3888 0.52 -1.5 4,318,448 + Pf1N1B4_3888 0.52 +0.4 4,318,448 + Pf1N1B4_3888 0.52 +2.2 4,318,449 - Pf1N1B4_3888 0.52 +1.5 4,318,449 - Pf1N1B4_3888 0.52 +0.8 4,318,449 - Pf1N1B4_3888 0.52 +0.8 4,318,449 - Pf1N1B4_3888 0.52 -1.2 4,318,449 - Pf1N1B4_3888 0.52 -2.0 4,318,449 - Pf1N1B4_3888 0.52 -1.6 4,318,730 + Pf1N1B4_3888 0.62 +1.2 4,318,730 + Pf1N1B4_3888 0.62 +0.8 4,318,730 + +0.8 4,318,731 - Pf1N1B4_3888 0.62 -1.8 4,318,731 - Pf1N1B4_3888 0.62 -0.2 4,318,732 + Pf1N1B4_3888 0.63 +0.4 4,318,733 - Pf1N1B4_3888 0.63 +0.2 4,318,733 - +2.5 4,318,733 - Pf1N1B4_3888 0.63 +0.2 4,318,784 - Pf1N1B4_3888 0.64 +1.5 4,319,155 + Pf1N1B4_3888 0.78 -0.4 4,319,155 + Pf1N1B4_3888 0.78 +0.2 4,319,155 + Pf1N1B4_3888 0.78 +0.8 4,319,155 + Pf1N1B4_3888 0.78 +0.4 4,319,155 + Pf1N1B4_3888 0.78 +1.9 4,319,155 + Pf1N1B4_3888 0.78 +0.8 4,319,156 - Pf1N1B4_3888 0.78 -0.1 4,319,156 - Pf1N1B4_3888 0.78 -0.5 4,319,156 - Pf1N1B4_3888 0.78 -0.5 4,319,156 - Pf1N1B4_3888 0.78 -1.6 4,319,156 - Pf1N1B4_3888 0.78 +0.2 4,319,156 - Pf1N1B4_3888 0.78 -2.2 4,319,156 - Pf1N1B4_3888 0.78 -2.7 4,319,201 - Pf1N1B4_3888 0.80 +2.0 4,319,201 - Pf1N1B4_3888 0.80 +0.8 4,319,259 - Pf1N1B4_3888 0.82 -0.2 4,319,353 + +0.8 4,319,353 + Pf1N1B4_3888 0.85 +2.4 4,319,354 - Pf1N1B4_3888 0.85 -1.0 4,319,354 - Pf1N1B4_3888 0.85 -0.8 4,319,502 + +0.2 4,319,701 + -0.2 4,319,701 + +1.5 4,319,701 + +0.4 4,319,702 - +1.9 4,319,721 + +0.4 4,319,722 - -0.8 4,319,774 + +0.5 4,319,774 + -2.4 4,319,774 + -1.0 4,319,775 - -1.0 4,319,816 - -0.9 4,319,816 - -0.2 4,319,831 + +0.8 4,319,833 + +0.3 4,319,833 + +0.0 4,319,833 + -1.7 4,319,834 - -0.7 4,319,847 + Pf1N1B4_3889 0.15 -0.2 4,319,859 - Pf1N1B4_3889 0.21 -2.9 4,319,977 + Pf1N1B4_3889 0.86 +1.4 4,319,977 + Pf1N1B4_3889 0.86 -0.8 4,319,996 + -1.8
Or see this region's nucleotide sequence