Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3524 and Pf1N1B4_3525 overlap by 4 nucleotides Pf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotides Pf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides Pf1N1B4_3527 and Pf1N1B4_3528 are separated by 68 nucleotides
Pf1N1B4_3524 - FIG00955363: hypothetical protein, at 3,930,236 to 3,931,042
_3524
Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893
_3525
Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603
_3526
Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418
_3527
Pf1N1B4_3528 - Putative membrane protein, at 3,933,487 to 3,934,542
_3528
Position (kb)
3931
3932
3933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3931.299 kb on - strand, within Pf1N1B4_3525 at 3931.431 kb on + strand, within Pf1N1B4_3525 at 3931.516 kb on + strand at 3931.516 kb on + strand, within Pf1N1B4_3525 at 3931.516 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.630 kb on - strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.949 kb on - strand at 3931.949 kb on - strand at 3931.956 kb on + strand at 3931.956 kb on + strand at 3932.164 kb on - strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.403 kb on + strand, within Pf1N1B4_3526 at 3932.427 kb on + strand, within Pf1N1B4_3526 at 3932.608 kb on - strand at 3932.672 kb on - strand at 3932.849 kb on + strand at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3933.005 kb on + strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.034 kb on - strand, within Pf1N1B4_3527 at 3933.080 kb on + strand, within Pf1N1B4_3527 at 3933.081 kb on - strand, within Pf1N1B4_3527 at 3933.110 kb on + strand, within Pf1N1B4_3527 at 3933.351 kb on + strand at 3933.454 kb on - strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.485 kb on - strand at 3933.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 3,930,949 + Pf1N1B4_3524 0.88 +1.3 3,930,949 + Pf1N1B4_3524 0.88 -0.4 3,930,949 + Pf1N1B4_3524 0.88 +0.5 3,931,299 - Pf1N1B4_3525 0.30 +1.7 3,931,431 + Pf1N1B4_3525 0.46 -1.1 3,931,516 + +0.3 3,931,516 + Pf1N1B4_3525 0.56 +0.5 3,931,516 + Pf1N1B4_3525 0.56 -0.1 3,931,629 + Pf1N1B4_3525 0.69 -0.4 3,931,629 + Pf1N1B4_3525 0.69 +1.4 3,931,630 - -0.1 3,931,948 + +0.7 3,931,948 + +0.9 3,931,948 + +1.1 3,931,949 - -1.4 3,931,949 - +0.6 3,931,956 + -0.6 3,931,956 + +0.9 3,932,164 - Pf1N1B4_3526 0.33 -1.9 3,932,352 + Pf1N1B4_3526 0.62 +0.5 3,932,352 + Pf1N1B4_3526 0.62 -0.4 3,932,352 + Pf1N1B4_3526 0.62 -2.4 3,932,352 + Pf1N1B4_3526 0.62 -0.7 3,932,352 + Pf1N1B4_3526 0.62 -2.8 3,932,352 + Pf1N1B4_3526 0.62 -0.7 3,932,352 + Pf1N1B4_3526 0.62 -2.7 3,932,403 + Pf1N1B4_3526 0.70 +0.3 3,932,427 + Pf1N1B4_3526 0.73 +1.9 3,932,608 - +0.9 3,932,672 - -0.7 3,932,849 + +0.9 3,932,850 - Pf1N1B4_3527 0.29 +0.3 3,932,850 - Pf1N1B4_3527 0.29 -0.3 3,932,850 - Pf1N1B4_3527 0.29 -0.1 3,932,932 - Pf1N1B4_3527 0.40 -0.7 3,932,932 - Pf1N1B4_3527 0.40 -1.7 3,933,005 + Pf1N1B4_3527 0.49 +0.8 3,933,030 - Pf1N1B4_3527 0.52 -1.9 3,933,030 - Pf1N1B4_3527 0.52 -0.8 3,933,034 - Pf1N1B4_3527 0.52 +1.1 3,933,080 + Pf1N1B4_3527 0.58 -1.4 3,933,081 - Pf1N1B4_3527 0.58 -0.7 3,933,110 + Pf1N1B4_3527 0.62 +0.5 3,933,351 + +1.2 3,933,454 - +0.7 3,933,484 + -1.4 3,933,484 + -0.8 3,933,484 + -1.4 3,933,484 + -1.7 3,933,484 + +2.2 3,933,485 - +1.5 3,933,485 - +0.1
Or see this region's nucleotide sequence