Experiment: methylglyoxal 0.008 vol% 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_3139 and Pf1N1B4_3140 are separated by 253 nucleotides Pf1N1B4_3140 and Pf1N1B4_3141 overlap by 19 nucleotides Pf1N1B4_3141 and Pf1N1B4_3142 are separated by 80 nucleotides Pf1N1B4_3142 and Pf1N1B4_3143 are separated by 83 nucleotides 
        Pf1N1B4_3139 - Prophage tail fiber protein, at 3,522,593 to 3,523,801 
        _3139 
         
        
        Pf1N1B4_3140 - FIG101079: Lytic enzyme, at 3,524,055 to 3,524,585 
        _3140 
         
        
        Pf1N1B4_3141 - FIG00959768: hypothetical protein, at 3,524,567 to 3,525,085 
        _3141 
         
        
        Pf1N1B4_3142 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 3,525,166 to 3,525,666 
        _3142 
         
        
        Pf1N1B4_3143 - RecA protein, at 3,525,750 to 3,526,802 
        _3143 
         Position (kb) 3524 
3525 
3526 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 3523.593 kb on + strand, within Pf1N1B4_3139 at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.797 kb on + strand at 3523.798 kb on - strand at 3523.799 kb on + strand at 3523.799 kb on + strand at 3523.800 kb on - strand at 3523.820 kb on + strand at 3523.820 kb on + strand at 3523.827 kb on + strand at 3523.842 kb on + strand at 3523.842 kb on + strand at 3523.842 kb on + strand at 3523.843 kb on - strand at 3523.867 kb on + strand at 3523.905 kb on - strand at 3524.005 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.021 kb on + strand at 3524.022 kb on - strand at 3524.022 kb on - strand at 3524.022 kb on - strand at 3524.022 kb on - strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.044 kb on + strand at 3524.045 kb on - strand at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.185 kb on + strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.186 kb on - strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.190 kb on + strand, within Pf1N1B4_3140 at 3524.191 kb on - strand at 3524.191 kb on - strand, within Pf1N1B4_3140 at 3524.191 kb on - strand, within Pf1N1B4_3140 at 3524.191 kb on - strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.194 kb on + strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.195 kb on - strand, within Pf1N1B4_3140 at 3524.218 kb on - strand, within Pf1N1B4_3140 at 3524.229 kb on + strand, within Pf1N1B4_3140 at 3524.229 kb on + strand, within Pf1N1B4_3140 at 3524.229 kb on + strand at 3524.230 kb on - strand, within Pf1N1B4_3140 at 3524.230 kb on - strand, within Pf1N1B4_3140 at 3524.295 kb on + strand, within Pf1N1B4_3140 at 3524.296 kb on - strand, within Pf1N1B4_3140 at 3524.566 kb on + strand at 3524.566 kb on + strand at 3524.567 kb on - strand at 3524.711 kb on - strand, within Pf1N1B4_3141 at 3524.818 kb on + strand, within Pf1N1B4_3141 at 3524.819 kb on - strand, within Pf1N1B4_3141 at 3525.005 kb on - strand at 3525.005 kb on - strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.130 kb on - strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.316 kb on + strand, within Pf1N1B4_3142 at 3525.317 kb on - strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.473 kb on + strand, within Pf1N1B4_3142 at 3525.474 kb on - strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.728 kb on + strand at 3525.729 kb on - strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.939 kb on + strand, within Pf1N1B4_3143 at 3525.939 kb on + strand, within Pf1N1B4_3143  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction methylglyoxal 0.008 vol% remove 3,523,593 +  Pf1N1B4_3139 0.83  +1.1 3,523,797 +  -0.9 3,523,797 +  -0.5 3,523,797 +  +2.5 3,523,797 +  +1.1 3,523,797 +  +1.1 3,523,797 +  -1.5 3,523,797 +  +0.3 3,523,797 +  -0.7 3,523,797 +  +1.0 3,523,797 +  +1.7 3,523,797 +  -0.7 3,523,797 +  +1.0 3,523,797 +  -0.2 3,523,797 +  -0.7 3,523,798 -  -0.4 3,523,799 +  -0.5 3,523,799 +  -0.5 3,523,800 -  +1.1 3,523,820 +  +0.9 3,523,820 +  +1.1 3,523,827 +  -0.3 3,523,842 +  +1.1 3,523,842 +  +0.4 3,523,842 +  +0.8 3,523,843 -  +2.1 3,523,867 +  -1.9 3,523,905 -  +0.7 3,524,005 +  +1.0 3,524,021 +  -0.9 3,524,021 +  -0.1 3,524,021 +  -1.9 3,524,021 +  -0.9 3,524,021 +  +2.3 3,524,021 +  +2.0 3,524,021 +  +0.5 3,524,021 +  +1.1 3,524,022 -  -0.9 3,524,022 -  -0.8 3,524,022 -  -0.3 3,524,022 -  -1.2 3,524,044 +  +0.1 3,524,044 +  -0.3 3,524,044 +  -1.5 3,524,044 +  +1.8 3,524,044 +  +1.1 3,524,044 +  +0.6 3,524,045 -  -0.9 3,524,185 +  Pf1N1B4_3140 0.24  -0.5 3,524,185 +  Pf1N1B4_3140 0.24  +1.5 3,524,185 +  Pf1N1B4_3140 0.24  -0.7 3,524,185 +  Pf1N1B4_3140 0.24  -0.5 3,524,185 +  Pf1N1B4_3140 0.24  +0.1 3,524,185 +  Pf1N1B4_3140 0.24  +1.4 3,524,185 +  Pf1N1B4_3140 0.24  +0.5 3,524,186 -  Pf1N1B4_3140 0.25  +1.6 3,524,186 -  Pf1N1B4_3140 0.25  -0.9 3,524,186 -  Pf1N1B4_3140 0.25  +1.4 3,524,186 -  Pf1N1B4_3140 0.25  -1.2 3,524,186 -  Pf1N1B4_3140 0.25  +0.4 3,524,186 -  Pf1N1B4_3140 0.25  +0.7 3,524,186 -  Pf1N1B4_3140 0.25  +0.3 3,524,186 -  Pf1N1B4_3140 0.25  -1.2 3,524,190 +  Pf1N1B4_3140 0.25  -0.5 3,524,190 +  Pf1N1B4_3140 0.25  -0.3 3,524,190 +  Pf1N1B4_3140 0.25  -0.9 3,524,190 +  Pf1N1B4_3140 0.25  +1.1 3,524,190 +  Pf1N1B4_3140 0.25  -0.9 3,524,191 -  +0.5 3,524,191 -  Pf1N1B4_3140 0.26  +0.8 3,524,191 -  Pf1N1B4_3140 0.26  +0.9 3,524,191 -  Pf1N1B4_3140 0.26  +0.5 3,524,194 +  Pf1N1B4_3140 0.26  -0.1 3,524,194 +  Pf1N1B4_3140 0.26  +0.1 3,524,194 +  Pf1N1B4_3140 0.26  -0.1 3,524,194 +  Pf1N1B4_3140 0.26  +1.5 3,524,194 +  Pf1N1B4_3140 0.26  -0.0 3,524,194 +  Pf1N1B4_3140 0.26  -0.7 3,524,194 +  Pf1N1B4_3140 0.26  +0.7 3,524,194 +  Pf1N1B4_3140 0.26  -1.0 3,524,194 +  Pf1N1B4_3140 0.26  -1.5 3,524,195 -  Pf1N1B4_3140 0.26  +0.5 3,524,195 -  Pf1N1B4_3140 0.26  +0.7 3,524,195 -  Pf1N1B4_3140 0.26  +1.5 3,524,195 -  Pf1N1B4_3140 0.26  -0.5 3,524,195 -  Pf1N1B4_3140 0.26  +0.5 3,524,195 -  Pf1N1B4_3140 0.26  -0.6 3,524,195 -  Pf1N1B4_3140 0.26  -1.2 3,524,195 -  Pf1N1B4_3140 0.26  +1.5 3,524,195 -  Pf1N1B4_3140 0.26  +0.1 3,524,218 -  Pf1N1B4_3140 0.31  -0.5 3,524,229 +  Pf1N1B4_3140 0.33  +0.1 3,524,229 +  Pf1N1B4_3140 0.33  +1.4 3,524,229 +  +1.5 3,524,230 -  Pf1N1B4_3140 0.33  -0.5 3,524,230 -  Pf1N1B4_3140 0.33  +0.5 3,524,295 +  Pf1N1B4_3140 0.45  +1.1 3,524,296 -  Pf1N1B4_3140 0.45  -1.2 3,524,566 +  +2.4 3,524,566 +  +0.8 3,524,567 -  -1.2 3,524,711 -  Pf1N1B4_3141 0.28  -0.6 3,524,818 +  Pf1N1B4_3141 0.48  -0.4 3,524,819 -  Pf1N1B4_3141 0.49  -1.2 3,525,005 -  -1.2 3,525,005 -  -1.2 3,525,062 +  -0.4 3,525,062 +  -0.4 3,525,062 +  -0.8 3,525,062 +  -0.1 3,525,063 -  +0.7 3,525,063 -  -0.9 3,525,063 -  +0.9 3,525,063 -  +1.5 3,525,130 -  +0.4 3,525,174 +  -0.7 3,525,174 +  -0.1 3,525,174 +  -1.2 3,525,316 +  Pf1N1B4_3142 0.30  +0.3 3,525,317 -  Pf1N1B4_3142 0.30  +1.9 3,525,325 +  Pf1N1B4_3142 0.32  +0.5 3,525,325 +  Pf1N1B4_3142 0.32  -0.1 3,525,325 +  +1.1 3,525,326 -  Pf1N1B4_3142 0.32  +0.7 3,525,326 -  Pf1N1B4_3142 0.32  -1.2 3,525,326 -  Pf1N1B4_3142 0.32  -0.4 3,525,473 +  Pf1N1B4_3142 0.61  +1.1 3,525,474 -  Pf1N1B4_3142 0.61  +0.2 3,525,565 +  Pf1N1B4_3142 0.80  -0.9 3,525,565 +  Pf1N1B4_3142 0.80  -1.2 3,525,565 +  Pf1N1B4_3142 0.80  -1.3 3,525,728 +  -0.7 3,525,729 -  -1.4 3,525,735 +  +0.7 3,525,735 +  -0.0 3,525,735 +  +1.1 3,525,735 +  -0.2 3,525,939 +  Pf1N1B4_3143 0.18  -0.5 3,525,939 +  Pf1N1B4_3143 0.18  -1.5 
Or see this region's nucleotide sequence