Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_266 and Pf1N1B4_267 overlap by 42 nucleotides Pf1N1B4_267 and Pf1N1B4_268 are separated by 117 nucleotides Pf1N1B4_268 and Pf1N1B4_269 are separated by 151 nucleotides
Pf1N1B4_266 - hypothetical protein, at 326,859 to 327,038
_266
Pf1N1B4_267 - Enoyl-CoA hydratase (EC 4.2.1.17), at 326,997 to 327,788
_267
Pf1N1B4_268 - Spermidine synthase (EC 2.5.1.16), at 327,906 to 328,661
_268
Pf1N1B4_269 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54), at 328,813 to 330,159
_269
Position (kb) 327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 327.022 kb on + strand at 327.123 kb on + strand, within Pf1N1B4_267 at 327.124 kb on - strand at 327.324 kb on + strand, within Pf1N1B4_267 at 327.324 kb on + strand, within Pf1N1B4_267 at 327.494 kb on + strand, within Pf1N1B4_267 at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.796 kb on - strand at 327.907 kb on + strand at 327.907 kb on + strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 328.082 kb on - strand, within Pf1N1B4_268 at 328.231 kb on + strand, within Pf1N1B4_268 at 328.232 kb on - strand, within Pf1N1B4_268 at 328.232 kb on - strand, within Pf1N1B4_268 at 328.276 kb on - strand, within Pf1N1B4_268 at 328.303 kb on + strand, within Pf1N1B4_268 at 328.303 kb on + strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.333 kb on + strand, within Pf1N1B4_268 at 328.334 kb on - strand, within Pf1N1B4_268 at 328.502 kb on - strand, within Pf1N1B4_268 at 328.507 kb on + strand, within Pf1N1B4_268 at 328.507 kb on + strand, within Pf1N1B4_268 at 328.508 kb on - strand, within Pf1N1B4_268 at 328.623 kb on + strand at 328.623 kb on + strand at 328.624 kb on - strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.699 kb on + strand at 328.699 kb on + strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.765 kb on + strand at 328.765 kb on + strand at 328.765 kb on + strand at 328.766 kb on - strand at 328.766 kb on - strand at 328.775 kb on + strand at 328.780 kb on + strand at 328.781 kb on - strand at 328.781 kb on - strand at 328.794 kb on + strand at 328.795 kb on - strand at 328.802 kb on + strand at 329.067 kb on + strand, within Pf1N1B4_269 at 329.255 kb on + strand, within Pf1N1B4_269 at 329.256 kb on - strand, within Pf1N1B4_269 at 329.316 kb on - strand, within Pf1N1B4_269 at 329.316 kb on - strand, within Pf1N1B4_269 at 329.316 kb on - strand, within Pf1N1B4_269 at 329.316 kb on - strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.407 kb on + strand, within Pf1N1B4_269 at 329.408 kb on - strand, within Pf1N1B4_269 at 329.408 kb on - strand, within Pf1N1B4_269 at 329.408 kb on - strand, within Pf1N1B4_269 at 329.530 kb on + strand, within Pf1N1B4_269 at 329.530 kb on + strand at 329.530 kb on + strand, within Pf1N1B4_269 at 329.572 kb on + strand, within Pf1N1B4_269 at 329.573 kb on - strand, within Pf1N1B4_269 at 329.575 kb on + strand at 329.575 kb on + strand, within Pf1N1B4_269 at 329.575 kb on + strand, within Pf1N1B4_269 at 329.575 kb on + strand, within Pf1N1B4_269 at 329.576 kb on - strand, within Pf1N1B4_269 at 329.576 kb on - strand, within Pf1N1B4_269 at 329.576 kb on - strand, within Pf1N1B4_269
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 327,022 + -0.2 327,123 + Pf1N1B4_267 0.16 +1.2 327,124 - -0.2 327,324 + Pf1N1B4_267 0.41 -0.2 327,324 + Pf1N1B4_267 0.41 -2.2 327,494 + Pf1N1B4_267 0.63 +0.2 327,795 + +0.3 327,795 + +0.8 327,795 + -0.8 327,795 + -0.0 327,795 + -0.0 327,795 + +0.2 327,795 + -0.6 327,795 + +0.2 327,795 + +1.8 327,796 - +0.2 327,907 + +0.8 327,907 + +0.2 327,908 - +0.4 327,908 - -0.8 327,908 - -0.2 327,908 - -0.2 327,908 - +2.3 327,908 - +0.0 328,082 - Pf1N1B4_268 0.23 +1.1 328,231 + Pf1N1B4_268 0.43 -0.8 328,232 - Pf1N1B4_268 0.43 -0.8 328,232 - Pf1N1B4_268 0.43 -0.8 328,276 - Pf1N1B4_268 0.49 -1.6 328,303 + Pf1N1B4_268 0.53 +0.2 328,303 + Pf1N1B4_268 0.53 +2.7 328,304 - Pf1N1B4_268 0.53 +0.2 328,304 - Pf1N1B4_268 0.53 -1.7 328,304 - Pf1N1B4_268 0.53 -0.0 328,304 - Pf1N1B4_268 0.53 -2.0 328,333 + Pf1N1B4_268 0.56 -2.3 328,334 - Pf1N1B4_268 0.57 -2.3 328,502 - Pf1N1B4_268 0.79 -0.2 328,507 + Pf1N1B4_268 0.79 -0.2 328,507 + Pf1N1B4_268 0.79 +0.2 328,508 - Pf1N1B4_268 0.80 +0.2 328,623 + +0.4 328,623 + -0.8 328,624 - +0.5 328,696 + +0.8 328,696 + -1.2 328,696 + +0.8 328,696 + +0.2 328,696 + +0.1 328,697 - -0.2 328,697 - -0.8 328,697 - -1.2 328,697 - -0.5 328,697 - -2.8 328,697 - +1.0 328,697 - +0.8 328,697 - +1.5 328,697 - -0.8 328,699 + +1.2 328,699 + +0.8 328,700 - +0.6 328,700 - +0.2 328,700 - +0.5 328,700 - +0.6 328,700 - +1.4 328,700 - -1.6 328,700 - -0.6 328,700 - +0.3 328,765 + -2.2 328,765 + +2.8 328,765 + -0.0 328,766 - +0.8 328,766 - -0.6 328,775 + -2.2 328,780 + +1.2 328,781 - -1.2 328,781 - +0.2 328,794 + -1.8 328,795 - -1.8 328,802 + -0.8 329,067 + Pf1N1B4_269 0.19 -0.2 329,255 + Pf1N1B4_269 0.33 -0.0 329,256 - Pf1N1B4_269 0.33 +0.2 329,316 - Pf1N1B4_269 0.37 -2.2 329,316 - Pf1N1B4_269 0.37 +0.4 329,316 - Pf1N1B4_269 0.37 -1.3 329,316 - Pf1N1B4_269 0.37 -1.5 329,407 + Pf1N1B4_269 0.44 -0.9 329,407 + Pf1N1B4_269 0.44 +0.4 329,407 + Pf1N1B4_269 0.44 -0.3 329,407 + Pf1N1B4_269 0.44 -1.5 329,407 + Pf1N1B4_269 0.44 +1.2 329,407 + Pf1N1B4_269 0.44 -1.2 329,407 + Pf1N1B4_269 0.44 +1.7 329,408 - Pf1N1B4_269 0.44 -0.4 329,408 - Pf1N1B4_269 0.44 +1.1 329,408 - Pf1N1B4_269 0.44 -1.2 329,530 + Pf1N1B4_269 0.53 +1.0 329,530 + +2.8 329,530 + Pf1N1B4_269 0.53 -0.7 329,572 + Pf1N1B4_269 0.56 -2.8 329,573 - Pf1N1B4_269 0.56 +0.8 329,575 + +2.6 329,575 + Pf1N1B4_269 0.57 +1.2 329,575 + Pf1N1B4_269 0.57 +0.1 329,575 + Pf1N1B4_269 0.57 -0.2 329,576 - Pf1N1B4_269 0.57 +0.0 329,576 - Pf1N1B4_269 0.57 -0.5 329,576 - Pf1N1B4_269 0.57 +3.4
Or see this region's nucleotide sequence