Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2506 and Pf1N1B4_2507 are separated by 117 nucleotides Pf1N1B4_2507 and Pf1N1B4_2508 are separated by 574 nucleotides Pf1N1B4_2508 and Pf1N1B4_2509 are separated by 453 nucleotides Pf1N1B4_2509 and Pf1N1B4_2510 are separated by 66 nucleotides Pf1N1B4_2510 and Pf1N1B4_2511 are separated by 4 nucleotides
Pf1N1B4_2506 - Transcriptional regulator, AsnC family, at 2,843,742 to 2,844,203
_2506
Pf1N1B4_2507 - hypothetical protein, at 2,844,321 to 2,844,482
_2507
Pf1N1B4_2508 - hypothetical protein, at 2,845,057 to 2,845,365
_2508
Pf1N1B4_2509 - hypothetical protein, at 2,845,819 to 2,845,962
_2509
Pf1N1B4_2510 - hypothetical protein, at 2,846,029 to 2,846,262
_2510
Pf1N1B4_2511 - hypothetical protein, at 2,846,267 to 2,846,749
_2511
Position (kb)
2845
2846 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2844.244 kb on + strand at 2844.244 kb on + strand at 2844.245 kb on - strand at 2844.245 kb on - strand at 2844.299 kb on + strand at 2844.300 kb on - strand at 2844.322 kb on + strand at 2844.322 kb on + strand at 2844.322 kb on + strand at 2844.372 kb on + strand, within Pf1N1B4_2507 at 2844.373 kb on - strand, within Pf1N1B4_2507 at 2844.373 kb on - strand, within Pf1N1B4_2507 at 2844.408 kb on + strand, within Pf1N1B4_2507 at 2844.408 kb on + strand, within Pf1N1B4_2507 at 2844.409 kb on - strand, within Pf1N1B4_2507 at 2844.409 kb on - strand, within Pf1N1B4_2507 at 2844.415 kb on + strand at 2844.497 kb on + strand at 2844.497 kb on + strand at 2844.497 kb on + strand at 2844.556 kb on + strand at 2844.592 kb on + strand at 2844.592 kb on + strand at 2844.627 kb on - strand at 2844.688 kb on + strand at 2844.689 kb on - strand at 2844.689 kb on - strand at 2844.716 kb on + strand at 2844.716 kb on + strand at 2844.716 kb on + strand at 2844.716 kb on + strand at 2844.779 kb on + strand at 2844.780 kb on - strand at 2844.903 kb on - strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.907 kb on - strand at 2844.907 kb on - strand at 2844.907 kb on - strand at 2844.985 kb on - strand at 2845.148 kb on + strand, within Pf1N1B4_2508 at 2845.148 kb on + strand, within Pf1N1B4_2508 at 2845.149 kb on - strand, within Pf1N1B4_2508 at 2845.149 kb on - strand, within Pf1N1B4_2508 at 2845.149 kb on - strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.303 kb on + strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.371 kb on + strand at 2845.371 kb on + strand at 2845.371 kb on + strand at 2845.372 kb on - strand at 2845.525 kb on + strand at 2845.532 kb on - strand at 2845.532 kb on - strand at 2845.607 kb on - strand at 2845.681 kb on + strand at 2845.682 kb on - strand at 2845.727 kb on + strand at 2845.727 kb on + strand at 2845.728 kb on - strand at 2845.728 kb on - strand at 2845.728 kb on - strand at 2845.728 kb on - strand at 2845.728 kb on - strand at 2845.730 kb on - strand at 2845.818 kb on + strand at 2845.818 kb on + strand at 2845.819 kb on - strand at 2845.819 kb on - strand at 2845.819 kb on - strand at 2845.819 kb on - strand at 2845.819 kb on - strand at 2845.819 kb on - strand at 2845.826 kb on + strand at 2845.826 kb on + strand at 2845.827 kb on - strand at 2845.827 kb on - strand at 2845.827 kb on - strand at 2845.827 kb on - strand at 2845.878 kb on - strand, within Pf1N1B4_2509 at 2846.001 kb on + strand at 2846.002 kb on - strand at 2846.133 kb on + strand, within Pf1N1B4_2510 at 2846.133 kb on + strand, within Pf1N1B4_2510 at 2846.134 kb on - strand, within Pf1N1B4_2510 at 2846.134 kb on - strand, within Pf1N1B4_2510 at 2846.134 kb on - strand, within Pf1N1B4_2510 at 2846.140 kb on - strand, within Pf1N1B4_2510 at 2846.140 kb on - strand, within Pf1N1B4_2510 at 2846.204 kb on + strand, within Pf1N1B4_2510 at 2846.205 kb on - strand, within Pf1N1B4_2510 at 2846.231 kb on + strand, within Pf1N1B4_2510 at 2846.243 kb on + strand at 2846.243 kb on + strand at 2846.252 kb on + strand at 2846.253 kb on - strand at 2846.253 kb on - strand at 2846.253 kb on - strand at 2846.318 kb on + strand, within Pf1N1B4_2511 at 2846.319 kb on - strand, within Pf1N1B4_2511
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 2,844,244 + +2.2 2,844,244 + +0.7 2,844,245 - -1.8 2,844,245 - +0.6 2,844,299 + +0.6 2,844,300 - +0.4 2,844,322 + +1.5 2,844,322 + -1.6 2,844,322 + +0.6 2,844,372 + Pf1N1B4_2507 0.31 -0.0 2,844,373 - Pf1N1B4_2507 0.32 +1.6 2,844,373 - Pf1N1B4_2507 0.32 -1.8 2,844,408 + Pf1N1B4_2507 0.54 +0.0 2,844,408 + Pf1N1B4_2507 0.54 -0.4 2,844,409 - Pf1N1B4_2507 0.54 +0.1 2,844,409 - Pf1N1B4_2507 0.54 +2.6 2,844,415 + +2.4 2,844,497 + +1.0 2,844,497 + +1.1 2,844,497 + -1.9 2,844,556 + -0.8 2,844,592 + -0.2 2,844,592 + +0.1 2,844,627 - -0.9 2,844,688 + -0.9 2,844,689 - -0.3 2,844,689 - +0.3 2,844,716 + -0.0 2,844,716 + +0.0 2,844,716 + -0.5 2,844,716 + -0.3 2,844,779 + +0.1 2,844,780 - -0.9 2,844,903 - -0.5 2,844,906 + -0.9 2,844,906 + -1.6 2,844,906 + +1.4 2,844,906 + +1.2 2,844,906 + +0.6 2,844,906 + -0.9 2,844,906 + +1.0 2,844,906 + +0.7 2,844,906 + +0.0 2,844,907 - -1.4 2,844,907 - +0.0 2,844,907 - -0.2 2,844,985 - +0.4 2,845,148 + Pf1N1B4_2508 0.29 +1.8 2,845,148 + Pf1N1B4_2508 0.29 -1.4 2,845,149 - Pf1N1B4_2508 0.30 -0.5 2,845,149 - Pf1N1B4_2508 0.30 -0.8 2,845,149 - Pf1N1B4_2508 0.30 -1.3 2,845,301 + Pf1N1B4_2508 0.79 +1.8 2,845,301 + +1.4 2,845,301 + Pf1N1B4_2508 0.79 -0.4 2,845,301 + Pf1N1B4_2508 0.79 +1.1 2,845,301 + Pf1N1B4_2508 0.79 -0.6 2,845,301 + Pf1N1B4_2508 0.79 +0.1 2,845,301 + Pf1N1B4_2508 0.79 +0.2 2,845,301 + Pf1N1B4_2508 0.79 +0.7 2,845,301 + Pf1N1B4_2508 0.79 -1.8 2,845,301 + Pf1N1B4_2508 0.79 +0.4 2,845,301 + Pf1N1B4_2508 0.79 +1.4 2,845,301 + Pf1N1B4_2508 0.79 -1.2 2,845,301 + Pf1N1B4_2508 0.79 -0.5 2,845,301 + Pf1N1B4_2508 0.79 -0.9 2,845,302 - Pf1N1B4_2508 0.79 -1.9 2,845,302 - Pf1N1B4_2508 0.79 +1.2 2,845,302 - Pf1N1B4_2508 0.79 -1.9 2,845,302 - Pf1N1B4_2508 0.79 -0.9 2,845,302 - Pf1N1B4_2508 0.79 +2.0 2,845,302 - Pf1N1B4_2508 0.79 -0.4 2,845,302 - Pf1N1B4_2508 0.79 -0.5 2,845,302 - Pf1N1B4_2508 0.79 +0.8 2,845,302 - Pf1N1B4_2508 0.79 -0.6 2,845,302 - Pf1N1B4_2508 0.79 -0.7 2,845,302 - Pf1N1B4_2508 0.79 -0.3 2,845,302 - Pf1N1B4_2508 0.79 -0.8 2,845,302 - Pf1N1B4_2508 0.79 -1.2 2,845,302 - Pf1N1B4_2508 0.79 +0.1 2,845,302 - Pf1N1B4_2508 0.79 -1.4 2,845,302 - Pf1N1B4_2508 0.79 -0.9 2,845,303 + Pf1N1B4_2508 0.80 -0.2 2,845,304 - Pf1N1B4_2508 0.80 -2.5 2,845,304 - Pf1N1B4_2508 0.80 -1.6 2,845,304 - Pf1N1B4_2508 0.80 -0.6 2,845,304 - Pf1N1B4_2508 0.80 +0.7 2,845,371 + -0.6 2,845,371 + -0.2 2,845,371 + +0.5 2,845,372 - +2.4 2,845,525 + +1.0 2,845,532 - -0.3 2,845,532 - -0.5 2,845,607 - -1.4 2,845,681 + +0.6 2,845,682 - -0.1 2,845,727 + -0.2 2,845,727 + +0.2 2,845,728 - -2.1 2,845,728 - -1.1 2,845,728 - +1.4 2,845,728 - -0.1 2,845,728 - -0.0 2,845,730 - -0.4 2,845,818 + +0.6 2,845,818 + -0.6 2,845,819 - +1.1 2,845,819 - -0.5 2,845,819 - -0.2 2,845,819 - +0.4 2,845,819 - +1.4 2,845,819 - -1.6 2,845,826 + +0.7 2,845,826 + +0.7 2,845,827 - -0.2 2,845,827 - +2.1 2,845,827 - -0.2 2,845,827 - +0.8 2,845,878 - Pf1N1B4_2509 0.41 +1.0 2,846,001 + +2.4 2,846,002 - +0.1 2,846,133 + Pf1N1B4_2510 0.44 -1.2 2,846,133 + Pf1N1B4_2510 0.44 -0.9 2,846,134 - Pf1N1B4_2510 0.45 +0.3 2,846,134 - Pf1N1B4_2510 0.45 +0.7 2,846,134 - Pf1N1B4_2510 0.45 -1.8 2,846,140 - Pf1N1B4_2510 0.47 -0.9 2,846,140 - Pf1N1B4_2510 0.47 -1.4 2,846,204 + Pf1N1B4_2510 0.75 +0.1 2,846,205 - Pf1N1B4_2510 0.75 +1.4 2,846,231 + Pf1N1B4_2510 0.86 +0.1 2,846,243 + -0.9 2,846,243 + -0.5 2,846,252 + -0.2 2,846,253 - -0.9 2,846,253 - +1.2 2,846,253 - +0.2 2,846,318 + Pf1N1B4_2511 0.11 +2.1 2,846,319 - Pf1N1B4_2511 0.11 +0.4
Or see this region's nucleotide sequence