Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2460 and Pf1N1B4_2461 are separated by 205 nucleotides Pf1N1B4_2461 and Pf1N1B4_2462 are separated by 240 nucleotides Pf1N1B4_2462 and Pf1N1B4_2463 are separated by 1 nucleotides
Pf1N1B4_2460 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 2,790,447 to 2,791,304
_2460
Pf1N1B4_2461 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 2,791,510 to 2,792,709
_2461
Pf1N1B4_2462 - FIG00955037: hypothetical protein, at 2,792,950 to 2,793,567
_2462
Pf1N1B4_2463 - Acyltransferase family protein, at 2,793,569 to 2,794,453
_2463
Position (kb)
2791
2792
2793 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2790.546 kb on + strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.547 kb on - strand, within Pf1N1B4_2460 at 2790.722 kb on - strand, within Pf1N1B4_2460 at 2790.868 kb on - strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.966 kb on + strand, within Pf1N1B4_2460 at 2790.967 kb on - strand, within Pf1N1B4_2460 at 2791.077 kb on + strand, within Pf1N1B4_2460 at 2791.078 kb on - strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.116 kb on + strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.117 kb on - strand, within Pf1N1B4_2460 at 2791.178 kb on + strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.179 kb on - strand, within Pf1N1B4_2460 at 2791.302 kb on + strand at 2791.303 kb on - strand at 2791.363 kb on + strand at 2791.364 kb on - strand at 2791.364 kb on - strand at 2791.364 kb on - strand at 2791.482 kb on + strand at 2791.482 kb on + strand at 2791.483 kb on - strand at 2791.483 kb on - strand at 2791.483 kb on - strand at 2791.486 kb on + strand at 2791.486 kb on + strand at 2791.486 kb on + strand at 2791.486 kb on + strand at 2791.487 kb on - strand at 2791.487 kb on - strand at 2791.487 kb on - strand at 2791.487 kb on - strand at 2791.559 kb on + strand at 2791.680 kb on + strand, within Pf1N1B4_2461 at 2791.681 kb on - strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.833 kb on + strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.834 kb on - strand, within Pf1N1B4_2461 at 2791.879 kb on + strand, within Pf1N1B4_2461 at 2791.880 kb on - strand, within Pf1N1B4_2461 at 2791.880 kb on - strand, within Pf1N1B4_2461 at 2791.880 kb on - strand at 2791.880 kb on - strand, within Pf1N1B4_2461 at 2791.925 kb on - strand, within Pf1N1B4_2461 at 2791.939 kb on + strand, within Pf1N1B4_2461 at 2791.939 kb on + strand, within Pf1N1B4_2461 at 2792.080 kb on + strand, within Pf1N1B4_2461 at 2792.080 kb on + strand, within Pf1N1B4_2461 at 2792.081 kb on - strand at 2792.298 kb on - strand, within Pf1N1B4_2461 at 2792.416 kb on + strand, within Pf1N1B4_2461 at 2792.539 kb on + strand, within Pf1N1B4_2461 at 2792.539 kb on + strand, within Pf1N1B4_2461 at 2792.539 kb on + strand, within Pf1N1B4_2461 at 2792.540 kb on - strand, within Pf1N1B4_2461 at 2792.540 kb on - strand, within Pf1N1B4_2461 at 2792.540 kb on - strand, within Pf1N1B4_2461 at 2792.638 kb on + strand at 2792.707 kb on + strand at 2792.823 kb on + strand at 2792.841 kb on + strand at 2793.312 kb on + strand, within Pf1N1B4_2462 at 2793.478 kb on + strand, within Pf1N1B4_2462 at 2793.479 kb on - strand, within Pf1N1B4_2462 at 2793.479 kb on - strand, within Pf1N1B4_2462 at 2793.557 kb on + strand at 2793.557 kb on + strand at 2793.557 kb on + strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.558 kb on - strand at 2793.561 kb on + strand at 2793.562 kb on - strand at 2793.562 kb on - strand at 2793.669 kb on + strand, within Pf1N1B4_2463
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 2,790,546 + Pf1N1B4_2460 0.12 +1.1 2,790,547 - Pf1N1B4_2460 0.12 +2.0 2,790,547 - Pf1N1B4_2460 0.12 +0.7 2,790,722 - Pf1N1B4_2460 0.32 -1.6 2,790,868 - Pf1N1B4_2460 0.49 -0.0 2,790,966 + Pf1N1B4_2460 0.60 +0.9 2,790,966 + Pf1N1B4_2460 0.60 +0.9 2,790,966 + Pf1N1B4_2460 0.60 -0.6 2,790,966 + Pf1N1B4_2460 0.60 +1.2 2,790,967 - Pf1N1B4_2460 0.61 +1.4 2,791,077 + Pf1N1B4_2460 0.73 +0.7 2,791,078 - Pf1N1B4_2460 0.74 -1.0 2,791,116 + Pf1N1B4_2460 0.78 -0.9 2,791,116 + Pf1N1B4_2460 0.78 +0.5 2,791,116 + Pf1N1B4_2460 0.78 +0.1 2,791,116 + Pf1N1B4_2460 0.78 +0.8 2,791,117 - Pf1N1B4_2460 0.78 +0.4 2,791,117 - Pf1N1B4_2460 0.78 +1.9 2,791,117 - Pf1N1B4_2460 0.78 +0.7 2,791,117 - Pf1N1B4_2460 0.78 +1.2 2,791,178 + Pf1N1B4_2460 0.85 +1.8 2,791,179 - Pf1N1B4_2460 0.85 +0.4 2,791,179 - Pf1N1B4_2460 0.85 +1.0 2,791,179 - Pf1N1B4_2460 0.85 +0.1 2,791,179 - Pf1N1B4_2460 0.85 +0.6 2,791,179 - Pf1N1B4_2460 0.85 -0.2 2,791,179 - Pf1N1B4_2460 0.85 +2.1 2,791,302 + -2.4 2,791,303 - -0.5 2,791,363 + -1.2 2,791,364 - -0.2 2,791,364 - +0.6 2,791,364 - +0.9 2,791,482 + +1.2 2,791,482 + -0.7 2,791,483 - +2.4 2,791,483 - -1.2 2,791,483 - -0.9 2,791,486 + +1.0 2,791,486 + -0.7 2,791,486 + +0.2 2,791,486 + -0.2 2,791,487 - +1.2 2,791,487 - -0.6 2,791,487 - -0.9 2,791,487 - -0.2 2,791,559 + -2.0 2,791,680 + Pf1N1B4_2461 0.14 +0.2 2,791,681 - Pf1N1B4_2461 0.14 +0.5 2,791,833 + Pf1N1B4_2461 0.27 -0.5 2,791,833 + Pf1N1B4_2461 0.27 -1.3 2,791,833 + Pf1N1B4_2461 0.27 +0.4 2,791,833 + Pf1N1B4_2461 0.27 +0.2 2,791,834 - Pf1N1B4_2461 0.27 +0.6 2,791,834 - Pf1N1B4_2461 0.27 -1.6 2,791,834 - Pf1N1B4_2461 0.27 +1.4 2,791,834 - Pf1N1B4_2461 0.27 -0.2 2,791,834 - Pf1N1B4_2461 0.27 +1.0 2,791,834 - Pf1N1B4_2461 0.27 +0.4 2,791,834 - Pf1N1B4_2461 0.27 +0.2 2,791,879 + Pf1N1B4_2461 0.31 -0.6 2,791,880 - Pf1N1B4_2461 0.31 -2.6 2,791,880 - Pf1N1B4_2461 0.31 -0.6 2,791,880 - +0.4 2,791,880 - Pf1N1B4_2461 0.31 -0.2 2,791,925 - Pf1N1B4_2461 0.35 +1.6 2,791,939 + Pf1N1B4_2461 0.36 +0.9 2,791,939 + Pf1N1B4_2461 0.36 -0.1 2,792,080 + Pf1N1B4_2461 0.47 -0.6 2,792,080 + Pf1N1B4_2461 0.47 -0.9 2,792,081 - +1.4 2,792,298 - Pf1N1B4_2461 0.66 -0.7 2,792,416 + Pf1N1B4_2461 0.76 -0.6 2,792,539 + Pf1N1B4_2461 0.86 -0.9 2,792,539 + Pf1N1B4_2461 0.86 -0.9 2,792,539 + Pf1N1B4_2461 0.86 -0.2 2,792,540 - Pf1N1B4_2461 0.86 +1.0 2,792,540 - Pf1N1B4_2461 0.86 -1.2 2,792,540 - Pf1N1B4_2461 0.86 -1.8 2,792,638 + +0.4 2,792,707 + -0.9 2,792,823 + -1.4 2,792,841 + +2.1 2,793,312 + Pf1N1B4_2462 0.59 -0.8 2,793,478 + Pf1N1B4_2462 0.85 +0.7 2,793,479 - Pf1N1B4_2462 0.86 +0.2 2,793,479 - Pf1N1B4_2462 0.86 -1.2 2,793,557 + -0.4 2,793,557 + -0.4 2,793,557 + +1.1 2,793,558 - +0.2 2,793,558 - +0.1 2,793,558 - -0.9 2,793,558 - +3.7 2,793,558 - -0.6 2,793,558 - +0.3 2,793,561 + +1.0 2,793,562 - -0.6 2,793,562 - +0.8 2,793,669 + Pf1N1B4_2463 0.11 +1.7
Or see this region's nucleotide sequence