Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2159 and Pf1N1B4_2160 overlap by 1 nucleotides Pf1N1B4_2160 and Pf1N1B4_2161 are separated by 34 nucleotides Pf1N1B4_2161 and Pf1N1B4_2162 are separated by 112 nucleotides
Pf1N1B4_2159 - Benzoate transport protein, at 2,473,309 to 2,474,559
_2159
Pf1N1B4_2160 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 2,474,559 to 2,475,413
_2160
Pf1N1B4_2161 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 2,475,448 to 2,476,713
_2161
Pf1N1B4_2162 - MG(2+) CHELATASE FAMILY PROTEIN / ComM-related protein, at 2,476,826 to 2,478,319
_2162
Position (kb)
2475
2476
2477 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2474.468 kb on - strand at 2474.508 kb on - strand at 2474.541 kb on + strand at 2474.766 kb on + strand, within Pf1N1B4_2160 at 2474.767 kb on - strand, within Pf1N1B4_2160 at 2475.011 kb on + strand, within Pf1N1B4_2160 at 2475.011 kb on + strand, within Pf1N1B4_2160 at 2475.011 kb on + strand, within Pf1N1B4_2160 at 2475.012 kb on - strand, within Pf1N1B4_2160 at 2475.033 kb on + strand, within Pf1N1B4_2160 at 2475.033 kb on + strand, within Pf1N1B4_2160 at 2475.034 kb on - strand, within Pf1N1B4_2160 at 2475.034 kb on - strand, within Pf1N1B4_2160 at 2475.034 kb on - strand, within Pf1N1B4_2160 at 2475.070 kb on - strand, within Pf1N1B4_2160 at 2475.163 kb on + strand, within Pf1N1B4_2160 at 2475.164 kb on - strand, within Pf1N1B4_2160 at 2475.333 kb on + strand at 2475.333 kb on + strand at 2475.333 kb on + strand at 2475.334 kb on - strand at 2475.334 kb on - strand at 2475.336 kb on + strand at 2475.336 kb on + strand at 2475.411 kb on + strand at 2475.411 kb on + strand at 2475.412 kb on - strand at 2475.412 kb on - strand at 2475.412 kb on - strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.421 kb on + strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.422 kb on - strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.447 kb on + strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.448 kb on - strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.451 kb on + strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.452 kb on - strand at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.606 kb on + strand, within Pf1N1B4_2161 at 2475.607 kb on - strand, within Pf1N1B4_2161 at 2475.607 kb on - strand, within Pf1N1B4_2161 at 2475.607 kb on - strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.610 kb on + strand, within Pf1N1B4_2161 at 2475.611 kb on - strand, within Pf1N1B4_2161 at 2475.637 kb on + strand, within Pf1N1B4_2161 at 2475.637 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.640 kb on + strand, within Pf1N1B4_2161 at 2475.641 kb on - strand, within Pf1N1B4_2161 at 2475.655 kb on + strand, within Pf1N1B4_2161 at 2475.656 kb on - strand, within Pf1N1B4_2161 at 2475.715 kb on + strand, within Pf1N1B4_2161 at 2475.865 kb on + strand, within Pf1N1B4_2161 at 2475.914 kb on - strand, within Pf1N1B4_2161 at 2475.931 kb on - strand, within Pf1N1B4_2161 at 2476.196 kb on - strand, within Pf1N1B4_2161 at 2476.309 kb on + strand, within Pf1N1B4_2161 at 2476.432 kb on + strand, within Pf1N1B4_2161 at 2476.432 kb on + strand, within Pf1N1B4_2161 at 2476.432 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.471 kb on + strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.472 kb on - strand, within Pf1N1B4_2161 at 2476.705 kb on + strand at 2476.720 kb on + strand at 2476.721 kb on - strand at 2476.721 kb on - strand at 2476.731 kb on - strand at 2476.731 kb on - strand at 2476.733 kb on + strand at 2476.734 kb on - strand at 2476.827 kb on + strand at 2476.827 kb on + strand at 2476.827 kb on + strand at 2476.827 kb on + strand at 2476.827 kb on + strand at 2476.828 kb on - strand at 2476.889 kb on + strand at 2476.889 kb on + strand at 2476.889 kb on + strand at 2476.890 kb on - strand at 2476.890 kb on - strand at 2476.890 kb on - strand at 2476.890 kb on - strand at 2477.236 kb on + strand, within Pf1N1B4_2162 at 2477.237 kb on - strand, within Pf1N1B4_2162 at 2477.237 kb on - strand, within Pf1N1B4_2162 at 2477.237 kb on - strand, within Pf1N1B4_2162 at 2477.237 kb on - strand, within Pf1N1B4_2162 at 2477.248 kb on + strand, within Pf1N1B4_2162 at 2477.248 kb on + strand, within Pf1N1B4_2162 at 2477.248 kb on + strand, within Pf1N1B4_2162 at 2477.248 kb on + strand, within Pf1N1B4_2162 at 2477.248 kb on + strand, within Pf1N1B4_2162 at 2477.249 kb on - strand, within Pf1N1B4_2162 at 2477.249 kb on - strand, within Pf1N1B4_2162 at 2477.249 kb on - strand, within Pf1N1B4_2162 at 2477.382 kb on - strand, within Pf1N1B4_2162 at 2477.390 kb on + strand, within Pf1N1B4_2162 at 2477.390 kb on + strand, within Pf1N1B4_2162 at 2477.390 kb on + strand, within Pf1N1B4_2162 at 2477.391 kb on - strand, within Pf1N1B4_2162 at 2477.546 kb on + strand, within Pf1N1B4_2162 at 2477.547 kb on - strand, within Pf1N1B4_2162 at 2477.683 kb on + strand, within Pf1N1B4_2162 at 2477.683 kb on + strand, within Pf1N1B4_2162 at 2477.684 kb on - strand, within Pf1N1B4_2162 at 2477.684 kb on - strand, within Pf1N1B4_2162 at 2477.684 kb on - strand, within Pf1N1B4_2162 at 2477.684 kb on - strand, within Pf1N1B4_2162 at 2477.684 kb on - strand, within Pf1N1B4_2162
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 2,474,468 - -1.8 2,474,508 - -2.0 2,474,541 + -0.4 2,474,766 + Pf1N1B4_2160 0.24 -1.3 2,474,767 - Pf1N1B4_2160 0.24 +1.1 2,475,011 + Pf1N1B4_2160 0.53 +0.7 2,475,011 + Pf1N1B4_2160 0.53 +0.6 2,475,011 + Pf1N1B4_2160 0.53 -1.8 2,475,012 - Pf1N1B4_2160 0.53 +1.7 2,475,033 + Pf1N1B4_2160 0.55 +2.2 2,475,033 + Pf1N1B4_2160 0.55 +0.6 2,475,034 - Pf1N1B4_2160 0.56 +1.0 2,475,034 - Pf1N1B4_2160 0.56 +0.6 2,475,034 - Pf1N1B4_2160 0.56 -1.4 2,475,070 - Pf1N1B4_2160 0.60 -0.0 2,475,163 + Pf1N1B4_2160 0.71 +0.7 2,475,164 - Pf1N1B4_2160 0.71 +0.6 2,475,333 + -0.0 2,475,333 + -0.8 2,475,333 + +1.6 2,475,334 - +1.6 2,475,334 - -1.0 2,475,336 + +0.2 2,475,336 + -1.3 2,475,411 + -0.6 2,475,411 + +1.1 2,475,412 - -0.3 2,475,412 - -0.3 2,475,412 - -0.8 2,475,421 + -2.4 2,475,421 + -0.2 2,475,421 + -0.5 2,475,421 + +1.7 2,475,421 + +0.8 2,475,421 + +0.7 2,475,422 - -0.4 2,475,422 - +0.3 2,475,422 - -0.4 2,475,422 - +0.8 2,475,422 - -0.4 2,475,447 + -0.0 2,475,447 + +1.7 2,475,447 + -1.4 2,475,447 + -1.9 2,475,447 + +2.2 2,475,447 + +2.0 2,475,447 + +0.2 2,475,447 + +1.1 2,475,447 + -1.3 2,475,447 + -0.4 2,475,447 + +0.9 2,475,447 + +0.6 2,475,447 + +0.2 2,475,447 + -0.4 2,475,447 + -0.0 2,475,447 + +0.6 2,475,447 + +1.3 2,475,447 + -2.0 2,475,447 + -0.8 2,475,448 - -1.0 2,475,448 - -0.0 2,475,448 - -1.0 2,475,448 - -1.6 2,475,448 - +1.2 2,475,448 - +0.7 2,475,448 - +0.3 2,475,448 - +1.2 2,475,448 - -0.3 2,475,448 - -0.0 2,475,448 - -0.8 2,475,448 - +0.8 2,475,448 - +0.2 2,475,448 - +1.0 2,475,448 - +0.9 2,475,448 - -0.4 2,475,451 + +0.9 2,475,451 + +1.2 2,475,451 + -0.1 2,475,451 + +0.2 2,475,451 + -1.3 2,475,451 + +1.3 2,475,451 + +2.3 2,475,451 + +0.6 2,475,451 + +0.6 2,475,451 + +0.6 2,475,452 - -0.3 2,475,452 - +2.1 2,475,452 - +1.1 2,475,452 - -1.7 2,475,452 - -0.1 2,475,452 - -1.4 2,475,452 - +0.2 2,475,452 - -1.6 2,475,452 - -0.4 2,475,606 + Pf1N1B4_2161 0.12 -0.6 2,475,606 + Pf1N1B4_2161 0.12 +1.6 2,475,606 + Pf1N1B4_2161 0.12 +1.0 2,475,606 + Pf1N1B4_2161 0.12 +1.6 2,475,607 - Pf1N1B4_2161 0.13 -1.0 2,475,607 - Pf1N1B4_2161 0.13 +2.2 2,475,607 - Pf1N1B4_2161 0.13 -1.4 2,475,610 + Pf1N1B4_2161 0.13 +0.1 2,475,610 + Pf1N1B4_2161 0.13 -1.0 2,475,610 + Pf1N1B4_2161 0.13 -0.6 2,475,610 + Pf1N1B4_2161 0.13 -1.4 2,475,611 - Pf1N1B4_2161 0.13 -1.0 2,475,637 + Pf1N1B4_2161 0.15 +0.1 2,475,637 + Pf1N1B4_2161 0.15 -2.1 2,475,640 + Pf1N1B4_2161 0.15 +1.0 2,475,640 + Pf1N1B4_2161 0.15 +0.6 2,475,640 + Pf1N1B4_2161 0.15 -1.0 2,475,640 + Pf1N1B4_2161 0.15 +1.1 2,475,641 - Pf1N1B4_2161 0.15 -0.0 2,475,655 + Pf1N1B4_2161 0.16 +1.1 2,475,656 - Pf1N1B4_2161 0.16 +0.7 2,475,715 + Pf1N1B4_2161 0.21 +0.7 2,475,865 + Pf1N1B4_2161 0.33 +0.8 2,475,914 - Pf1N1B4_2161 0.37 -0.7 2,475,931 - Pf1N1B4_2161 0.38 -0.4 2,476,196 - Pf1N1B4_2161 0.59 +0.9 2,476,309 + Pf1N1B4_2161 0.68 +0.3 2,476,432 + Pf1N1B4_2161 0.78 -0.0 2,476,432 + Pf1N1B4_2161 0.78 -0.0 2,476,432 + Pf1N1B4_2161 0.78 -1.4 2,476,471 + Pf1N1B4_2161 0.81 +0.8 2,476,471 + Pf1N1B4_2161 0.81 +2.1 2,476,471 + Pf1N1B4_2161 0.81 +1.8 2,476,471 + Pf1N1B4_2161 0.81 -0.4 2,476,471 + Pf1N1B4_2161 0.81 -0.2 2,476,471 + Pf1N1B4_2161 0.81 -1.3 2,476,471 + Pf1N1B4_2161 0.81 +0.1 2,476,471 + Pf1N1B4_2161 0.81 +0.8 2,476,471 + Pf1N1B4_2161 0.81 +0.3 2,476,471 + Pf1N1B4_2161 0.81 -1.6 2,476,471 + Pf1N1B4_2161 0.81 +2.1 2,476,471 + Pf1N1B4_2161 0.81 +1.7 2,476,471 + Pf1N1B4_2161 0.81 -0.4 2,476,471 + Pf1N1B4_2161 0.81 +1.7 2,476,472 - Pf1N1B4_2161 0.81 -1.0 2,476,472 - Pf1N1B4_2161 0.81 -1.3 2,476,472 - Pf1N1B4_2161 0.81 +0.5 2,476,472 - Pf1N1B4_2161 0.81 -1.0 2,476,472 - Pf1N1B4_2161 0.81 -1.3 2,476,472 - Pf1N1B4_2161 0.81 -0.1 2,476,472 - Pf1N1B4_2161 0.81 -1.3 2,476,472 - Pf1N1B4_2161 0.81 +1.1 2,476,705 + -0.0 2,476,720 + -1.0 2,476,721 - -0.0 2,476,721 - -0.6 2,476,731 - -1.3 2,476,731 - -1.4 2,476,733 + -2.0 2,476,734 - +2.4 2,476,827 + +1.8 2,476,827 + -0.0 2,476,827 + -1.0 2,476,827 + -1.6 2,476,827 + +0.1 2,476,828 - -0.9 2,476,889 + -1.0 2,476,889 + +0.3 2,476,889 + -2.0 2,476,890 - +1.1 2,476,890 - +0.2 2,476,890 - -1.4 2,476,890 - -0.0 2,477,236 + Pf1N1B4_2162 0.27 -1.6 2,477,237 - Pf1N1B4_2162 0.28 -2.5 2,477,237 - Pf1N1B4_2162 0.28 +0.1 2,477,237 - Pf1N1B4_2162 0.28 +2.3 2,477,237 - Pf1N1B4_2162 0.28 +2.1 2,477,248 + Pf1N1B4_2162 0.28 -1.9 2,477,248 + Pf1N1B4_2162 0.28 -0.4 2,477,248 + Pf1N1B4_2162 0.28 +0.7 2,477,248 + Pf1N1B4_2162 0.28 -1.0 2,477,248 + Pf1N1B4_2162 0.28 -1.0 2,477,249 - Pf1N1B4_2162 0.28 +1.0 2,477,249 - Pf1N1B4_2162 0.28 -0.0 2,477,249 - Pf1N1B4_2162 0.28 -0.0 2,477,382 - Pf1N1B4_2162 0.37 +1.4 2,477,390 + Pf1N1B4_2162 0.38 -0.8 2,477,390 + Pf1N1B4_2162 0.38 -0.8 2,477,390 + Pf1N1B4_2162 0.38 -1.3 2,477,391 - Pf1N1B4_2162 0.38 +1.1 2,477,546 + Pf1N1B4_2162 0.48 +0.6 2,477,547 - Pf1N1B4_2162 0.48 +0.7 2,477,683 + Pf1N1B4_2162 0.57 -0.9 2,477,683 + Pf1N1B4_2162 0.57 +1.5 2,477,684 - Pf1N1B4_2162 0.57 -1.2 2,477,684 - Pf1N1B4_2162 0.57 -1.3 2,477,684 - Pf1N1B4_2162 0.57 -0.6 2,477,684 - Pf1N1B4_2162 0.57 +0.9 2,477,684 - Pf1N1B4_2162 0.57 +0.1
Or see this region's nucleotide sequence