Experiment: methylglyoxal 0.008 vol% 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_1786 and Pf1N1B4_1787 are separated by 228 nucleotides Pf1N1B4_1787 and Pf1N1B4_1788 are separated by 177 nucleotides Pf1N1B4_1788 and Pf1N1B4_1789 are separated by 69 nucleotides  
        
        Pf1N1B4_1786 - ATP-dependent DNA helicase, RecQ family, at 2,038,340 to 2,040,274 
         
        _1786 
         
        
        Pf1N1B4_1787 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,040,503 to 2,041,729 
         
        _1787 
         
        
        Pf1N1B4_1788 - putative 4-hydroxybenzoyl-CoA thioesterase, at 2,041,907 to 2,042,338 
         
        _1788 
         
        
        Pf1N1B4_1789 - FIG01270322: hypothetical protein, at 2,042,408 to 2,042,896 
         
        _1789 
         Position (kb)  
2040 
 
2041 
 
2042 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 2039.546 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.552 kb on + strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.553 kb on - strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.576 kb on + strand, within Pf1N1B4_1786 at 2039.577 kb on - strand at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.577 kb on - strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.699 kb on + strand, within Pf1N1B4_1786 at 2039.700 kb on - strand, within Pf1N1B4_1786 at 2039.708 kb on + strand, within Pf1N1B4_1786 at 2039.709 kb on - strand, within Pf1N1B4_1786 at 2039.747 kb on + strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.748 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.802 kb on - strand, within Pf1N1B4_1786 at 2039.858 kb on + strand, within Pf1N1B4_1786 at 2039.859 kb on - strand, within Pf1N1B4_1786 at 2039.954 kb on + strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2039.955 kb on - strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.075 kb on + strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.076 kb on - strand, within Pf1N1B4_1786 at 2040.175 kb on + strand at 2040.176 kb on - strand at 2040.225 kb on - strand at 2040.401 kb on - strand at 2040.401 kb on - strand at 2040.462 kb on - strand at 2040.462 kb on - strand at 2040.488 kb on - strand at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.665 kb on + strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.666 kb on - strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.868 kb on + strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2040.869 kb on - strand, within Pf1N1B4_1787 at 2041.021 kb on - strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.103 kb on + strand, within Pf1N1B4_1787 at 2041.104 kb on - strand, within Pf1N1B4_1787 at 2041.155 kb on + strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.156 kb on - strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.205 kb on + strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.206 kb on - strand, within Pf1N1B4_1787 at 2041.337 kb on + strand, within Pf1N1B4_1787 at 2041.338 kb on - strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.340 kb on + strand, within Pf1N1B4_1787 at 2041.341 kb on - strand, within Pf1N1B4_1787 at 2041.547 kb on + strand, within Pf1N1B4_1787 at 2041.547 kb on + strand, within Pf1N1B4_1787 at 2041.548 kb on - strand, within Pf1N1B4_1787 at 2041.622 kb on - strand at 2041.734 kb on - strand at 2041.734 kb on - strand at 2041.734 kb on - strand at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.985 kb on + strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2041.986 kb on - strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.009 kb on + strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.010 kb on - strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.012 kb on + strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.013 kb on - strand, within Pf1N1B4_1788 at 2042.042 kb on + strand, within Pf1N1B4_1788 at 2042.042 kb on + strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.043 kb on - strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.117 kb on + strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.118 kb on - strand, within Pf1N1B4_1788 at 2042.196 kb on - strand, within Pf1N1B4_1788 at 2042.383 kb on - strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.428 kb on + strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.429 kb on - strand at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.503 kb on - strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.553 kb on + strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.554 kb on - strand, within Pf1N1B4_1789 at 2042.641 kb on + strand, within Pf1N1B4_1789  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   methylglyoxal 0.008 vol%             remove   2,039,546   +    Pf1N1B4_1786   0.62  +1.0   2,039,552   +    Pf1N1B4_1786   0.63  +1.1   2,039,552   +    Pf1N1B4_1786   0.63  +1.1   2,039,553   -    Pf1N1B4_1786   0.63  -1.6   2,039,553   -    Pf1N1B4_1786   0.63  -1.5   2,039,553   -    Pf1N1B4_1786   0.63  +0.2   2,039,576   +    Pf1N1B4_1786   0.64  +1.5   2,039,576   +    Pf1N1B4_1786   0.64  +0.5   2,039,576   +    Pf1N1B4_1786   0.64  -0.3   2,039,576   +    Pf1N1B4_1786   0.64  -0.7   2,039,576   +    Pf1N1B4_1786   0.64  -0.5   2,039,577   -        +0.5   2,039,577   -    Pf1N1B4_1786   0.64  -0.0   2,039,577   -    Pf1N1B4_1786   0.64  +2.3   2,039,577   -    Pf1N1B4_1786   0.64  -0.5   2,039,699   +    Pf1N1B4_1786   0.70  +2.3   2,039,699   +    Pf1N1B4_1786   0.70  -0.2   2,039,700   -    Pf1N1B4_1786   0.70  +0.8   2,039,708   +    Pf1N1B4_1786   0.71  -2.3   2,039,709   -    Pf1N1B4_1786   0.71  -0.1   2,039,747   +    Pf1N1B4_1786   0.73  -0.8   2,039,748   -    Pf1N1B4_1786   0.73  +0.1   2,039,748   -    Pf1N1B4_1786   0.73  +1.0   2,039,802   -    Pf1N1B4_1786   0.76  -0.0   2,039,802   -    Pf1N1B4_1786   0.76  -0.3   2,039,802   -    Pf1N1B4_1786   0.76  -0.6   2,039,858   +    Pf1N1B4_1786   0.78  -1.5   2,039,859   -    Pf1N1B4_1786   0.79  +0.2   2,039,954   +    Pf1N1B4_1786   0.83  -0.5   2,039,955   -    Pf1N1B4_1786   0.83  -0.0   2,039,955   -    Pf1N1B4_1786   0.83  -1.3   2,039,955   -    Pf1N1B4_1786   0.83  -2.0   2,040,075   +    Pf1N1B4_1786   0.90  +0.1   2,040,075   +        +1.5   2,040,075   +    Pf1N1B4_1786   0.90  -0.5   2,040,075   +    Pf1N1B4_1786   0.90  +1.0   2,040,075   +    Pf1N1B4_1786   0.90  +0.5   2,040,075   +    Pf1N1B4_1786   0.90  +1.1   2,040,076   -    Pf1N1B4_1786   0.90  +0.5   2,040,076   -    Pf1N1B4_1786   0.90  +1.1   2,040,076   -    Pf1N1B4_1786   0.90  -0.5   2,040,175   +        +1.0   2,040,176   -        -1.0   2,040,225   -        -0.8   2,040,401   -        +0.2   2,040,401   -        -1.5   2,040,462   -        +1.5   2,040,462   -        -0.0   2,040,488   -        +1.2   2,040,665   +    Pf1N1B4_1787   0.13  -0.0   2,040,665   +    Pf1N1B4_1787   0.13  +2.1   2,040,666   -    Pf1N1B4_1787   0.13  +0.3   2,040,666   -    Pf1N1B4_1787   0.13  -0.4   2,040,666   -    Pf1N1B4_1787   0.13  -0.0   2,040,666   -    Pf1N1B4_1787   0.13  -0.8   2,040,868   +    Pf1N1B4_1787   0.30  -0.5   2,040,868   +    Pf1N1B4_1787   0.30  +0.3   2,040,868   +    Pf1N1B4_1787   0.30  +1.5   2,040,868   +    Pf1N1B4_1787   0.30  -1.0   2,040,868   +    Pf1N1B4_1787   0.30  +1.5   2,040,869   -    Pf1N1B4_1787   0.30  -1.3   2,040,869   -    Pf1N1B4_1787   0.30  +1.8   2,041,021   -    Pf1N1B4_1787   0.42  +1.5   2,041,103   +    Pf1N1B4_1787   0.49  +1.0   2,041,103   +    Pf1N1B4_1787   0.49  -0.6   2,041,103   +    Pf1N1B4_1787   0.49  -0.3   2,041,104   -    Pf1N1B4_1787   0.49  +0.2   2,041,155   +    Pf1N1B4_1787   0.53  -0.0   2,041,156   -    Pf1N1B4_1787   0.53  +2.5   2,041,156   -    Pf1N1B4_1787   0.53  +0.2   2,041,156   -    Pf1N1B4_1787   0.53  +0.2   2,041,156   -    Pf1N1B4_1787   0.53  -0.8   2,041,205   +    Pf1N1B4_1787   0.57  +1.5   2,041,205   +    Pf1N1B4_1787   0.57  -1.6   2,041,205   +    Pf1N1B4_1787   0.57  +0.2   2,041,205   +    Pf1N1B4_1787   0.57  +1.2   2,041,205   +    Pf1N1B4_1787   0.57  +2.3   2,041,205   +    Pf1N1B4_1787   0.57  +1.1   2,041,206   -    Pf1N1B4_1787   0.57  -0.1   2,041,206   -    Pf1N1B4_1787   0.57  -1.0   2,041,337   +    Pf1N1B4_1787   0.68  +2.4   2,041,338   -    Pf1N1B4_1787   0.68  -1.5   2,041,340   +    Pf1N1B4_1787   0.68  +0.7   2,041,340   +    Pf1N1B4_1787   0.68  +1.8   2,041,340   +    Pf1N1B4_1787   0.68  +1.5   2,041,341   -    Pf1N1B4_1787   0.68  -0.1   2,041,547   +    Pf1N1B4_1787   0.85  -1.6   2,041,547   +    Pf1N1B4_1787   0.85  +0.5   2,041,548   -    Pf1N1B4_1787   0.85  +1.1   2,041,622   -        -0.8   2,041,734   -        -0.5   2,041,734   -        +0.1   2,041,734   -        -0.8   2,041,985   +    Pf1N1B4_1788   0.18  +0.5   2,041,985   +    Pf1N1B4_1788   0.18  -0.3   2,041,985   +    Pf1N1B4_1788   0.18  +0.4   2,041,985   +    Pf1N1B4_1788   0.18  +0.5   2,041,985   +    Pf1N1B4_1788   0.18  +0.4   2,041,985   +    Pf1N1B4_1788   0.18  -0.5   2,041,985   +    Pf1N1B4_1788   0.18  -0.5   2,041,985   +    Pf1N1B4_1788   0.18  -0.1   2,041,986   -    Pf1N1B4_1788   0.18  +0.5   2,041,986   -    Pf1N1B4_1788   0.18  +0.5   2,041,986   -        -0.8   2,041,986   -    Pf1N1B4_1788   0.18  -0.1   2,041,986   -    Pf1N1B4_1788   0.18  -1.1   2,042,009   +    Pf1N1B4_1788   0.24  -0.2   2,042,009   +    Pf1N1B4_1788   0.24  +1.5   2,042,009   +    Pf1N1B4_1788   0.24  -0.6   2,042,009   +    Pf1N1B4_1788   0.24  +2.3   2,042,010   -    Pf1N1B4_1788   0.24  +0.9   2,042,010   -    Pf1N1B4_1788   0.24  +0.9   2,042,010   -    Pf1N1B4_1788   0.24  +2.3   2,042,010   -    Pf1N1B4_1788   0.24  -0.1   2,042,010   -    Pf1N1B4_1788   0.24  -0.1   2,042,010   -    Pf1N1B4_1788   0.24  -0.8   2,042,012   +    Pf1N1B4_1788   0.24  -0.1   2,042,012   +    Pf1N1B4_1788   0.24  +0.1   2,042,012   +    Pf1N1B4_1788   0.24  +1.5   2,042,012   +    Pf1N1B4_1788   0.24  +0.4   2,042,013   -    Pf1N1B4_1788   0.25  -2.2   2,042,013   -    Pf1N1B4_1788   0.25  +0.9   2,042,013   -    Pf1N1B4_1788   0.25  -1.8   2,042,042   +    Pf1N1B4_1788   0.31  +2.0   2,042,042   +    Pf1N1B4_1788   0.31  +0.5   2,042,043   -    Pf1N1B4_1788   0.31  +0.4   2,042,043   -    Pf1N1B4_1788   0.31  -0.8   2,042,043   -    Pf1N1B4_1788   0.31  +0.5   2,042,043   -    Pf1N1B4_1788   0.31  +0.7   2,042,117   +    Pf1N1B4_1788   0.49  -1.9   2,042,117   +    Pf1N1B4_1788   0.49  +0.5   2,042,117   +    Pf1N1B4_1788   0.49  -0.8   2,042,118   -    Pf1N1B4_1788   0.49  +0.1   2,042,118   -    Pf1N1B4_1788   0.49  +0.1   2,042,118   -    Pf1N1B4_1788   0.49  -1.5   2,042,118   -    Pf1N1B4_1788   0.49  +2.1   2,042,196   -    Pf1N1B4_1788   0.67  -0.1   2,042,383   -        +1.5   2,042,428   +        -0.8   2,042,428   +        -0.2   2,042,428   +        +0.9   2,042,428   +        -0.8   2,042,429   -        -0.1   2,042,429   -        -3.0   2,042,429   -        -0.2   2,042,429   -        +0.5   2,042,429   -        -0.7   2,042,429   -        -0.1   2,042,503   -    Pf1N1B4_1789   0.19  -0.3   2,042,503   -    Pf1N1B4_1789   0.19  -0.9   2,042,503   -    Pf1N1B4_1789   0.19  -2.9   2,042,553   +    Pf1N1B4_1789   0.30  -1.9   2,042,553   +    Pf1N1B4_1789   0.30  -0.3   2,042,553   +    Pf1N1B4_1789   0.30  +0.3   2,042,553   +    Pf1N1B4_1789   0.30  -0.7   2,042,553   +    Pf1N1B4_1789   0.30  -0.5   2,042,553   +    Pf1N1B4_1789   0.30  -0.6   2,042,553   +    Pf1N1B4_1789   0.30  +0.2   2,042,554   -    Pf1N1B4_1789   0.30  +0.2   2,042,554   -    Pf1N1B4_1789   0.30  +0.1   2,042,554   -    Pf1N1B4_1789   0.30  -0.3   2,042,554   -    Pf1N1B4_1789   0.30  -0.6   2,042,554   -    Pf1N1B4_1789   0.30  -0.4   2,042,554   -    Pf1N1B4_1789   0.30  +1.0   2,042,554   -    Pf1N1B4_1789   0.30  -1.3   2,042,554   -    Pf1N1B4_1789   0.30  +2.2   2,042,554   -    Pf1N1B4_1789   0.30  -1.1   2,042,554   -    Pf1N1B4_1789   0.30  +1.5   2,042,554   -    Pf1N1B4_1789   0.30  +0.5   2,042,554   -    Pf1N1B4_1789   0.30  +0.2   2,042,554   -    Pf1N1B4_1789   0.30  +1.2   2,042,554   -    Pf1N1B4_1789   0.30  -1.6   2,042,641   +    Pf1N1B4_1789   0.48  +0.9 
 
Or see this region's nucleotide sequence