Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_174

Experiment: methylglyoxal 0.008 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_172 and Pf1N1B4_173 overlap by 4 nucleotidesPf1N1B4_173 and Pf1N1B4_174 overlap by 11 nucleotidesPf1N1B4_174 and Pf1N1B4_175 overlap by 1 nucleotides Pf1N1B4_172 - Probable alkylhalidase homolog (EC 3.8.1.1), at 213,495 to 214,790 _172 Pf1N1B4_173 - Extracellular protease, at 214,787 to 215,464 _173 Pf1N1B4_174 - Lipid A export ATP-binding/permease protein MsbA, at 215,454 to 217,172 _174 Pf1N1B4_175 - sigma-54 dependent transcriptional regulator, at 217,172 to 219,076 _175 Position (kb) 215 216 217 218Strain fitness (log2 ratio) -1 0 1at 214.540 kb on - strand, within Pf1N1B4_172at 215.833 kb on + strand, within Pf1N1B4_174at 216.005 kb on + strand, within Pf1N1B4_174at 216.236 kb on - strand, within Pf1N1B4_174at 216.281 kb on - strand, within Pf1N1B4_174at 216.439 kb on + strand, within Pf1N1B4_174at 216.440 kb on - strand, within Pf1N1B4_174at 216.440 kb on - strand, within Pf1N1B4_174at 216.440 kb on - strand, within Pf1N1B4_174at 216.440 kb on - strand, within Pf1N1B4_174at 216.440 kb on - strand, within Pf1N1B4_174at 216.802 kb on + strand, within Pf1N1B4_174at 217.100 kb on - strandat 217.190 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol%
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214,540 - Pf1N1B4_172 0.81 -1.0
215,833 + Pf1N1B4_174 0.22 -1.6
216,005 + Pf1N1B4_174 0.32 -1.6
216,236 - Pf1N1B4_174 0.45 +0.2
216,281 - Pf1N1B4_174 0.48 -0.8
216,439 + Pf1N1B4_174 0.57 +1.5
216,440 - Pf1N1B4_174 0.57 -1.6
216,440 - Pf1N1B4_174 0.57 -1.1
216,440 - Pf1N1B4_174 0.57 +1.2
216,440 - Pf1N1B4_174 0.57 -0.2
216,440 - Pf1N1B4_174 0.57 -0.8
216,802 + Pf1N1B4_174 0.78 +1.2
217,100 - -1.1
217,190 - -1.6

Or see this region's nucleotide sequence