Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_158 and Pf1N1B4_159 are separated by 26 nucleotides Pf1N1B4_159 and Pf1N1B4_160 are separated by 89 nucleotides Pf1N1B4_160 and Pf1N1B4_161 are separated by 198 nucleotides Pf1N1B4_161 and Pf1N1B4_162 are separated by 177 nucleotides
Pf1N1B4_158 - FIG00456158: hypothetical protein, at 200,783 to 201,112
_158
Pf1N1B4_159 - Putative threonine efflux protein, at 201,139 to 201,768
_159
Pf1N1B4_160 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 201,858 to 203,183
_160
Pf1N1B4_161 - hypothetical protein, at 203,382 to 203,504
_161
Pf1N1B4_162 - Aha1 domain superfamily, at 203,682 to 204,170
_162
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -2
-1
0
1
2 at 200.935 kb on + strand, within Pf1N1B4_158 at 200.935 kb on + strand, within Pf1N1B4_158 at 200.956 kb on - strand at 200.959 kb on - strand, within Pf1N1B4_158 at 200.959 kb on - strand, within Pf1N1B4_158 at 200.959 kb on - strand, within Pf1N1B4_158 at 201.090 kb on + strand at 201.091 kb on - strand at 201.095 kb on - strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.109 kb on - strand at 201.109 kb on - strand at 201.231 kb on + strand at 201.255 kb on + strand, within Pf1N1B4_159 at 201.256 kb on - strand, within Pf1N1B4_159 at 201.392 kb on - strand, within Pf1N1B4_159 at 201.665 kb on + strand, within Pf1N1B4_159 at 201.666 kb on - strand, within Pf1N1B4_159 at 201.797 kb on - strand at 201.797 kb on - strand at 201.801 kb on + strand at 201.801 kb on + strand at 201.840 kb on + strand at 202.002 kb on + strand, within Pf1N1B4_160 at 202.003 kb on - strand at 202.168 kb on + strand, within Pf1N1B4_160 at 202.169 kb on - strand, within Pf1N1B4_160 at 202.280 kb on - strand, within Pf1N1B4_160 at 202.280 kb on - strand, within Pf1N1B4_160 at 202.407 kb on + strand, within Pf1N1B4_160 at 202.534 kb on - strand, within Pf1N1B4_160 at 202.647 kb on + strand, within Pf1N1B4_160 at 202.648 kb on - strand, within Pf1N1B4_160 at 202.685 kb on + strand, within Pf1N1B4_160 at 202.685 kb on + strand, within Pf1N1B4_160 at 202.686 kb on - strand, within Pf1N1B4_160 at 202.686 kb on - strand, within Pf1N1B4_160 at 202.710 kb on + strand, within Pf1N1B4_160 at 202.710 kb on + strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.713 kb on - strand, within Pf1N1B4_160 at 202.772 kb on + strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.773 kb on - strand, within Pf1N1B4_160 at 202.977 kb on + strand, within Pf1N1B4_160 at 202.978 kb on - strand, within Pf1N1B4_160 at 202.978 kb on - strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.980 kb on + strand, within Pf1N1B4_160 at 202.981 kb on - strand, within Pf1N1B4_160 at 202.981 kb on - strand, within Pf1N1B4_160 at 203.008 kb on + strand at 203.043 kb on - strand, within Pf1N1B4_160 at 203.269 kb on + strand at 203.327 kb on + strand at 203.328 kb on - strand at 203.328 kb on - strand at 203.359 kb on + strand at 203.359 kb on + strand at 203.360 kb on - strand at 203.360 kb on - strand at 203.360 kb on - strand at 203.369 kb on + strand at 203.370 kb on - strand at 203.375 kb on + strand at 203.375 kb on + strand at 203.376 kb on - strand at 203.487 kb on - strand, within Pf1N1B4_161 at 203.540 kb on + strand at 203.540 kb on + strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.541 kb on - strand at 203.577 kb on + strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.578 kb on - strand at 203.581 kb on + strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.582 kb on - strand at 203.593 kb on + strand at 203.594 kb on - strand at 203.594 kb on - strand at 203.607 kb on + strand at 203.635 kb on + strand at 203.636 kb on - strand at 203.636 kb on - strand at 203.985 kb on + strand, within Pf1N1B4_162 at 203.986 kb on - strand, within Pf1N1B4_162 at 203.986 kb on - strand, within Pf1N1B4_162 at 204.047 kb on + strand, within Pf1N1B4_162 at 204.170 kb on + strand at 204.170 kb on + strand at 204.170 kb on + strand at 204.170 kb on + strand at 204.170 kb on + strand at 204.171 kb on - strand at 204.171 kb on - strand at 204.171 kb on - strand at 204.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 200,935 + Pf1N1B4_158 0.46 -0.6 200,935 + Pf1N1B4_158 0.46 -1.0 200,956 - +1.7 200,959 - Pf1N1B4_158 0.53 +0.6 200,959 - Pf1N1B4_158 0.53 +1.2 200,959 - Pf1N1B4_158 0.53 -0.1 201,090 + +0.0 201,091 - +0.6 201,095 - +0.7 201,108 + -1.3 201,108 + -2.6 201,108 + -0.4 201,108 + -2.4 201,108 + +0.8 201,109 - +0.7 201,109 - -0.1 201,231 + -1.3 201,255 + Pf1N1B4_159 0.18 -0.5 201,256 - Pf1N1B4_159 0.19 +0.7 201,392 - Pf1N1B4_159 0.40 -0.3 201,665 + Pf1N1B4_159 0.83 +0.5 201,666 - Pf1N1B4_159 0.84 -0.3 201,797 - -0.3 201,797 - +0.5 201,801 + -0.6 201,801 + +0.3 201,840 + +1.7 202,002 + Pf1N1B4_160 0.11 -0.2 202,003 - +2.0 202,168 + Pf1N1B4_160 0.23 +0.9 202,169 - Pf1N1B4_160 0.23 +1.0 202,280 - Pf1N1B4_160 0.32 -0.6 202,280 - Pf1N1B4_160 0.32 +0.9 202,407 + Pf1N1B4_160 0.41 -0.5 202,534 - Pf1N1B4_160 0.51 -0.8 202,647 + Pf1N1B4_160 0.60 +0.0 202,648 - Pf1N1B4_160 0.60 -0.6 202,685 + Pf1N1B4_160 0.62 -1.3 202,685 + Pf1N1B4_160 0.62 -1.8 202,686 - Pf1N1B4_160 0.62 -1.2 202,686 - Pf1N1B4_160 0.62 -0.7 202,710 + Pf1N1B4_160 0.64 +0.3 202,710 + Pf1N1B4_160 0.64 +1.1 202,711 - Pf1N1B4_160 0.64 +1.2 202,711 - Pf1N1B4_160 0.64 -2.6 202,711 - Pf1N1B4_160 0.64 -1.6 202,711 - Pf1N1B4_160 0.64 -1.3 202,711 - Pf1N1B4_160 0.64 +0.2 202,711 - Pf1N1B4_160 0.64 +0.1 202,711 - Pf1N1B4_160 0.64 +0.7 202,711 - Pf1N1B4_160 0.64 -0.6 202,711 - +0.2 202,711 - Pf1N1B4_160 0.64 +0.3 202,711 - Pf1N1B4_160 0.64 +0.2 202,713 - Pf1N1B4_160 0.64 +0.6 202,772 + Pf1N1B4_160 0.69 +0.3 202,773 - Pf1N1B4_160 0.69 +0.1 202,773 - Pf1N1B4_160 0.69 +2.3 202,773 - Pf1N1B4_160 0.69 +0.3 202,773 - Pf1N1B4_160 0.69 -0.3 202,977 + Pf1N1B4_160 0.84 +0.0 202,978 - Pf1N1B4_160 0.84 +0.4 202,978 - Pf1N1B4_160 0.84 -0.7 202,980 + Pf1N1B4_160 0.85 -0.6 202,980 + Pf1N1B4_160 0.85 +0.7 202,980 + Pf1N1B4_160 0.85 -2.1 202,980 + Pf1N1B4_160 0.85 +0.7 202,981 - Pf1N1B4_160 0.85 -2.6 202,981 - Pf1N1B4_160 0.85 +1.5 203,008 + -0.3 203,043 - Pf1N1B4_160 0.89 +1.9 203,269 + +0.2 203,327 + -0.7 203,328 - +1.7 203,328 - -1.8 203,359 + +1.3 203,359 + +0.7 203,360 - -1.4 203,360 - +0.3 203,360 - -0.8 203,369 + -0.4 203,370 - -1.4 203,375 + +0.7 203,375 + -0.3 203,376 - +0.3 203,487 - Pf1N1B4_161 0.85 -2.7 203,540 + +0.7 203,540 + +2.1 203,541 - -0.3 203,541 - +0.7 203,541 - -2.3 203,541 - -0.3 203,541 - -1.3 203,541 - -0.8 203,577 + +0.5 203,578 - +0.0 203,578 - +0.2 203,578 - -0.3 203,578 - -2.1 203,578 - -1.3 203,578 - +0.2 203,578 - -0.6 203,578 - +0.2 203,578 - -1.6 203,578 - +1.9 203,578 - +0.7 203,578 - +0.7 203,578 - +0.6 203,578 - +0.3 203,578 - -0.6 203,578 - -1.3 203,578 - +1.1 203,581 + -0.3 203,582 - -0.8 203,582 - -1.3 203,582 - -0.4 203,582 - -1.8 203,582 - -0.2 203,582 - +0.4 203,593 + +2.2 203,594 - -0.1 203,594 - +1.8 203,607 + +0.0 203,635 + +1.2 203,636 - -0.0 203,636 - +0.1 203,985 + Pf1N1B4_162 0.62 +0.2 203,986 - Pf1N1B4_162 0.62 -0.6 203,986 - Pf1N1B4_162 0.62 +0.4 204,047 + Pf1N1B4_162 0.75 -0.8 204,170 + +0.0 204,170 + -1.7 204,170 + +1.6 204,170 + -0.8 204,170 + -0.1 204,171 - +1.2 204,171 - -0.9 204,171 - -0.3 204,171 - +0.3
Or see this region's nucleotide sequence