Experiment: methylglyoxal 0.008 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1176 and Pf1N1B4_1177 are separated by 102 nucleotides Pf1N1B4_1177 and Pf1N1B4_1178 are separated by 241 nucleotides Pf1N1B4_1178 and Pf1N1B4_1179 are separated by 75 nucleotides Pf1N1B4_1179 and Pf1N1B4_1180 are separated by 97 nucleotides
Pf1N1B4_1176 - Chaperone protein DnaK, at 1,326,921 to 1,328,837
_1176
Pf1N1B4_1177 - Heat shock protein GrpE, at 1,328,940 to 1,329,506
_1177
Pf1N1B4_1178 - DNA repair protein RecN, at 1,329,748 to 1,331,421
_1178
Pf1N1B4_1179 - Ferric uptake regulation protein FUR, at 1,331,497 to 1,331,901
_1179
Pf1N1B4_1180 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 1,331,999 to 1,332,526
_1180
Position (kb)
1329
1330
1331
1332 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1328.902 kb on - strand at 1328.917 kb on - strand at 1328.917 kb on - strand at 1329.560 kb on - strand at 1329.562 kb on - strand at 1329.591 kb on + strand at 1329.714 kb on - strand at 1329.747 kb on + strand at 1329.748 kb on - strand at 1329.748 kb on - strand at 1329.767 kb on + strand at 1329.767 kb on + strand at 1329.767 kb on + strand at 1329.768 kb on - strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.771 kb on + strand at 1329.772 kb on - strand at 1329.772 kb on - strand at 1329.772 kb on - strand at 1329.772 kb on - strand at 1329.775 kb on + strand at 1329.775 kb on + strand at 1329.775 kb on + strand at 1330.192 kb on + strand, within Pf1N1B4_1178 at 1330.472 kb on + strand, within Pf1N1B4_1178 at 1330.472 kb on + strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.473 kb on - strand, within Pf1N1B4_1178 at 1330.598 kb on + strand, within Pf1N1B4_1178 at 1330.645 kb on + strand, within Pf1N1B4_1178 at 1330.672 kb on + strand, within Pf1N1B4_1178 at 1330.673 kb on - strand, within Pf1N1B4_1178 at 1330.673 kb on - strand, within Pf1N1B4_1178 at 1330.673 kb on - strand, within Pf1N1B4_1178 at 1330.734 kb on - strand, within Pf1N1B4_1178 at 1330.734 kb on - strand, within Pf1N1B4_1178 at 1330.804 kb on + strand, within Pf1N1B4_1178 at 1330.804 kb on + strand, within Pf1N1B4_1178 at 1330.804 kb on + strand, within Pf1N1B4_1178 at 1330.816 kb on + strand, within Pf1N1B4_1178 at 1330.883 kb on + strand, within Pf1N1B4_1178 at 1330.883 kb on + strand, within Pf1N1B4_1178 at 1330.947 kb on + strand, within Pf1N1B4_1178 at 1330.947 kb on + strand, within Pf1N1B4_1178 at 1330.986 kb on - strand, within Pf1N1B4_1178 at 1331.039 kb on + strand, within Pf1N1B4_1178 at 1331.064 kb on + strand, within Pf1N1B4_1178 at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.264 kb on + strand at 1331.265 kb on - strand at 1331.265 kb on - strand at 1331.295 kb on + strand at 1331.295 kb on + strand at 1331.296 kb on - strand at 1331.296 kb on - strand at 1331.419 kb on + strand at 1331.498 kb on + strand at 1331.499 kb on - strand at 1331.499 kb on - strand at 1332.373 kb on + strand, within Pf1N1B4_1180 at 1332.373 kb on + strand, within Pf1N1B4_1180
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.008 vol% remove 1,328,902 - +1.3 1,328,917 - +0.4 1,328,917 - -0.7 1,329,560 - -1.7 1,329,562 - +0.1 1,329,591 + -1.3 1,329,714 - -0.7 1,329,747 + -1.3 1,329,748 - -0.3 1,329,748 - +0.9 1,329,767 + +1.3 1,329,767 + -0.3 1,329,767 + +0.3 1,329,768 - -1.5 1,329,771 + +1.6 1,329,771 + -1.3 1,329,771 + -0.8 1,329,771 + -1.5 1,329,771 + -0.5 1,329,771 + +0.7 1,329,771 + -1.0 1,329,772 - +0.3 1,329,772 - -1.0 1,329,772 - -0.6 1,329,772 - +0.3 1,329,775 + -1.0 1,329,775 + -0.7 1,329,775 + -1.0 1,330,192 + Pf1N1B4_1178 0.27 +0.3 1,330,472 + Pf1N1B4_1178 0.43 -1.3 1,330,472 + Pf1N1B4_1178 0.43 +0.7 1,330,473 - Pf1N1B4_1178 0.43 -1.7 1,330,473 - Pf1N1B4_1178 0.43 +0.1 1,330,473 - Pf1N1B4_1178 0.43 -2.0 1,330,473 - Pf1N1B4_1178 0.43 -1.5 1,330,598 + Pf1N1B4_1178 0.51 +1.3 1,330,645 + Pf1N1B4_1178 0.54 -1.4 1,330,672 + Pf1N1B4_1178 0.55 +0.3 1,330,673 - Pf1N1B4_1178 0.55 -1.0 1,330,673 - Pf1N1B4_1178 0.55 -1.3 1,330,673 - Pf1N1B4_1178 0.55 -2.6 1,330,734 - Pf1N1B4_1178 0.59 +0.3 1,330,734 - Pf1N1B4_1178 0.59 +0.6 1,330,804 + Pf1N1B4_1178 0.63 -1.0 1,330,804 + Pf1N1B4_1178 0.63 +1.9 1,330,804 + Pf1N1B4_1178 0.63 +1.9 1,330,816 + Pf1N1B4_1178 0.64 -0.8 1,330,883 + Pf1N1B4_1178 0.68 -0.0 1,330,883 + Pf1N1B4_1178 0.68 +1.3 1,330,947 + Pf1N1B4_1178 0.72 -0.5 1,330,947 + Pf1N1B4_1178 0.72 +0.1 1,330,986 - Pf1N1B4_1178 0.74 -0.9 1,331,039 + Pf1N1B4_1178 0.77 -1.5 1,331,064 + Pf1N1B4_1178 0.79 -2.1 1,331,264 + -0.4 1,331,264 + -0.3 1,331,264 + -1.2 1,331,264 + +0.9 1,331,264 + -0.6 1,331,265 - -1.9 1,331,265 - -0.1 1,331,295 + -1.3 1,331,295 + +0.5 1,331,296 - -1.3 1,331,296 - -1.3 1,331,419 + -0.7 1,331,498 + -1.7 1,331,499 - -1.0 1,331,499 - +0.3 1,332,373 + Pf1N1B4_1180 0.71 -2.8 1,332,373 + Pf1N1B4_1180 0.71 -0.3
Or see this region's nucleotide sequence