Experiment: PYE with Paraquat dichloride 0.015 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00180 and CCNA_00181 overlap by 4 nucleotides CCNA_00181 and CCNA_00182 overlap by 4 nucleotides CCNA_00182 and CCNA_00183 are separated by 109 nucleotides CCNA_00183 and CCNA_00184 are separated by 58 nucleotides
CCNA_00180: CCNA_00180 - type II secretion pathway protein L, at 193,848 to 194,954
_00180
CCNA_00181: CCNA_00181 - type II secretion pathway protein M, at 194,951 to 195,427
_00181
CCNA_00182: CCNA_00182 - type II secretion pathway protein N, at 195,424 to 196,140
_00182
CCNA_00183: CCNA_00183 - type II secretory pathway, prepilin signal peptidase, at 196,250 to 197,011
_00183
CCNA_00184: CCNA_00184 - hypothetical protein, at 197,070 to 197,267
_00184
Position (kb) 195
196
197 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 194.456 kb on - strand, within CCNA_00180 at 194.510 kb on - strand, within CCNA_00180 at 194.540 kb on + strand, within CCNA_00180 at 194.546 kb on - strand, within CCNA_00180 at 194.548 kb on - strand, within CCNA_00180 at 194.638 kb on - strand, within CCNA_00180 at 194.640 kb on - strand, within CCNA_00180 at 194.671 kb on + strand, within CCNA_00180 at 194.671 kb on + strand, within CCNA_00180 at 194.677 kb on - strand, within CCNA_00180 at 194.679 kb on - strand, within CCNA_00180 at 194.719 kb on - strand, within CCNA_00180 at 194.752 kb on + strand, within CCNA_00180 at 194.758 kb on + strand, within CCNA_00180 at 194.777 kb on - strand, within CCNA_00180 at 194.779 kb on + strand, within CCNA_00180 at 194.779 kb on + strand, within CCNA_00180 at 194.779 kb on + strand, within CCNA_00180 at 194.787 kb on - strand, within CCNA_00180 at 194.787 kb on - strand, within CCNA_00180 at 194.787 kb on - strand, within CCNA_00180 at 194.847 kb on + strand at 194.869 kb on + strand at 194.875 kb on + strand at 194.883 kb on - strand at 194.907 kb on - strand at 194.918 kb on + strand at 194.948 kb on + strand at 194.949 kb on - strand at 194.956 kb on - strand at 194.962 kb on + strand at 194.962 kb on + strand at 194.962 kb on + strand at 194.962 kb on + strand at 195.048 kb on - strand, within CCNA_00181 at 195.057 kb on + strand, within CCNA_00181 at 195.088 kb on + strand, within CCNA_00181 at 195.111 kb on - strand, within CCNA_00181 at 195.111 kb on - strand, within CCNA_00181 at 195.111 kb on - strand, within CCNA_00181 at 195.130 kb on + strand, within CCNA_00181 at 195.138 kb on - strand, within CCNA_00181 at 195.210 kb on - strand, within CCNA_00181 at 195.273 kb on - strand, within CCNA_00181 at 195.310 kb on + strand, within CCNA_00181 at 195.310 kb on + strand, within CCNA_00181 at 195.310 kb on + strand, within CCNA_00181 at 195.310 kb on + strand, within CCNA_00181 at 195.313 kb on - strand, within CCNA_00181 at 195.318 kb on - strand, within CCNA_00181 at 195.331 kb on - strand, within CCNA_00181 at 195.358 kb on - strand, within CCNA_00181 at 195.388 kb on + strand at 195.396 kb on - strand at 195.396 kb on - strand at 195.408 kb on - strand at 195.442 kb on - strand at 195.464 kb on + strand at 195.468 kb on + strand at 195.475 kb on + strand at 195.524 kb on + strand, within CCNA_00182 at 195.524 kb on + strand, within CCNA_00182 at 195.529 kb on - strand, within CCNA_00182 at 195.530 kb on - strand, within CCNA_00182 at 195.547 kb on - strand, within CCNA_00182 at 195.549 kb on - strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.558 kb on + strand, within CCNA_00182 at 195.566 kb on - strand, within CCNA_00182 at 195.566 kb on - strand, within CCNA_00182 at 195.566 kb on - strand, within CCNA_00182 at 195.566 kb on - strand, within CCNA_00182 at 195.573 kb on + strand, within CCNA_00182 at 195.573 kb on + strand, within CCNA_00182 at 195.573 kb on + strand, within CCNA_00182 at 195.581 kb on - strand, within CCNA_00182 at 195.631 kb on - strand, within CCNA_00182 at 195.636 kb on + strand, within CCNA_00182 at 195.640 kb on - strand, within CCNA_00182 at 195.654 kb on + strand, within CCNA_00182 at 195.672 kb on - strand, within CCNA_00182 at 195.683 kb on - strand, within CCNA_00182 at 195.696 kb on + strand, within CCNA_00182 at 195.711 kb on - strand, within CCNA_00182 at 195.757 kb on + strand, within CCNA_00182 at 195.757 kb on + strand, within CCNA_00182 at 195.757 kb on + strand, within CCNA_00182 at 195.758 kb on + strand, within CCNA_00182 at 195.758 kb on + strand, within CCNA_00182 at 195.758 kb on + strand, within CCNA_00182 at 195.758 kb on + strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.765 kb on - strand, within CCNA_00182 at 195.825 kb on + strand, within CCNA_00182 at 195.825 kb on + strand, within CCNA_00182 at 195.882 kb on + strand, within CCNA_00182 at 195.892 kb on + strand, within CCNA_00182 at 195.892 kb on + strand, within CCNA_00182 at 195.914 kb on - strand, within CCNA_00182 at 195.916 kb on + strand, within CCNA_00182 at 195.937 kb on - strand, within CCNA_00182 at 195.985 kb on - strand, within CCNA_00182 at 195.985 kb on - strand, within CCNA_00182 at 195.995 kb on - strand, within CCNA_00182 at 195.996 kb on + strand, within CCNA_00182 at 196.004 kb on - strand, within CCNA_00182 at 196.028 kb on - strand, within CCNA_00182 at 196.046 kb on + strand, within CCNA_00182 at 196.051 kb on + strand, within CCNA_00182 at 196.059 kb on - strand, within CCNA_00182 at 196.114 kb on + strand at 196.246 kb on + strand at 196.259 kb on - strand at 196.261 kb on - strand at 196.275 kb on + strand at 196.275 kb on + strand at 196.275 kb on + strand at 196.278 kb on - strand at 196.283 kb on - strand at 196.361 kb on + strand, within CCNA_00183 at 196.361 kb on + strand, within CCNA_00183 at 196.362 kb on - strand, within CCNA_00183 at 196.369 kb on - strand, within CCNA_00183 at 196.369 kb on - strand, within CCNA_00183 at 196.403 kb on + strand, within CCNA_00183 at 196.406 kb on - strand, within CCNA_00183 at 196.406 kb on - strand, within CCNA_00183 at 196.408 kb on + strand, within CCNA_00183 at 196.462 kb on - strand, within CCNA_00183 at 196.478 kb on - strand, within CCNA_00183 at 196.500 kb on + strand, within CCNA_00183 at 196.500 kb on + strand, within CCNA_00183 at 196.500 kb on + strand, within CCNA_00183 at 196.511 kb on - strand, within CCNA_00183 at 196.530 kb on - strand, within CCNA_00183 at 196.553 kb on + strand, within CCNA_00183 at 196.553 kb on + strand, within CCNA_00183 at 196.553 kb on + strand, within CCNA_00183 at 196.576 kb on - strand, within CCNA_00183 at 196.619 kb on + strand, within CCNA_00183 at 196.619 kb on + strand, within CCNA_00183 at 196.632 kb on - strand, within CCNA_00183 at 196.656 kb on + strand, within CCNA_00183 at 196.699 kb on - strand, within CCNA_00183 at 196.772 kb on - strand, within CCNA_00183 at 196.776 kb on + strand, within CCNA_00183 at 196.779 kb on - strand, within CCNA_00183 at 196.784 kb on - strand, within CCNA_00183 at 196.814 kb on - strand, within CCNA_00183 at 196.814 kb on - strand, within CCNA_00183 at 196.833 kb on - strand, within CCNA_00183 at 196.851 kb on + strand, within CCNA_00183 at 196.868 kb on - strand, within CCNA_00183 at 196.901 kb on - strand, within CCNA_00183 at 196.913 kb on - strand, within CCNA_00183 at 196.989 kb on + strand at 196.989 kb on + strand at 196.997 kb on - strand at 196.997 kb on - strand at 196.997 kb on - strand at 196.997 kb on - strand at 196.997 kb on - strand at 197.014 kb on - strand at 197.015 kb on - strand at 197.017 kb on + strand at 197.027 kb on + strand at 197.027 kb on + strand at 197.035 kb on - strand at 197.043 kb on + strand at 197.043 kb on + strand at 197.051 kb on - strand at 197.051 kb on - strand at 197.099 kb on + strand, within CCNA_00184 at 197.119 kb on - strand, within CCNA_00184 at 197.121 kb on + strand, within CCNA_00184 at 197.129 kb on - strand, within CCNA_00184
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.015 mg/ml remove 194,456 - CCNA_00180 0.55 -0.5 194,510 - CCNA_00180 0.60 +0.1 194,540 + CCNA_00180 0.63 -2.1 194,546 - CCNA_00180 0.63 +0.0 194,548 - CCNA_00180 0.63 +1.0 194,638 - CCNA_00180 0.71 -0.2 194,640 - CCNA_00180 0.72 +1.1 194,671 + CCNA_00180 0.74 -0.8 194,671 + CCNA_00180 0.74 +0.3 194,677 - CCNA_00180 0.75 -1.4 194,679 - CCNA_00180 0.75 +0.5 194,719 - CCNA_00180 0.79 -1.0 194,752 + CCNA_00180 0.82 -0.3 194,758 + CCNA_00180 0.82 +0.4 194,777 - CCNA_00180 0.84 -0.9 194,779 + CCNA_00180 0.84 +0.3 194,779 + CCNA_00180 0.84 +1.0 194,779 + CCNA_00180 0.84 -1.5 194,787 - CCNA_00180 0.85 -2.6 194,787 - CCNA_00180 0.85 -0.1 194,787 - CCNA_00180 0.85 -0.4 194,847 + +3.0 194,869 + -2.3 194,875 + -0.5 194,883 - -2.1 194,907 - -0.8 194,918 + -2.0 194,948 + -1.4 194,949 - -0.6 194,956 - -0.6 194,962 + -1.2 194,962 + -0.4 194,962 + -0.9 194,962 + -0.5 195,048 - CCNA_00181 0.20 +0.7 195,057 + CCNA_00181 0.22 -0.2 195,088 + CCNA_00181 0.29 -1.6 195,111 - CCNA_00181 0.34 +0.8 195,111 - CCNA_00181 0.34 +1.2 195,111 - CCNA_00181 0.34 -0.5 195,130 + CCNA_00181 0.38 -0.9 195,138 - CCNA_00181 0.39 -1.1 195,210 - CCNA_00181 0.54 -2.9 195,273 - CCNA_00181 0.68 +0.0 195,310 + CCNA_00181 0.75 +0.4 195,310 + CCNA_00181 0.75 -0.3 195,310 + CCNA_00181 0.75 +1.0 195,310 + CCNA_00181 0.75 +2.6 195,313 - CCNA_00181 0.76 -2.2 195,318 - CCNA_00181 0.77 +0.4 195,331 - CCNA_00181 0.80 -2.8 195,358 - CCNA_00181 0.85 +0.3 195,388 + +0.3 195,396 - -1.7 195,396 - -0.5 195,408 - +1.4 195,442 - -0.3 195,464 + +1.4 195,468 + +0.5 195,475 + -1.6 195,524 + CCNA_00182 0.14 -2.9 195,524 + CCNA_00182 0.14 -0.6 195,529 - CCNA_00182 0.15 -1.2 195,530 - CCNA_00182 0.15 +0.5 195,547 - CCNA_00182 0.17 -2.8 195,549 - CCNA_00182 0.17 -2.7 195,558 + CCNA_00182 0.19 +1.6 195,558 + CCNA_00182 0.19 +0.1 195,558 + CCNA_00182 0.19 -1.7 195,558 + CCNA_00182 0.19 +0.7 195,558 + CCNA_00182 0.19 -0.2 195,558 + CCNA_00182 0.19 +0.3 195,558 + CCNA_00182 0.19 +0.5 195,558 + CCNA_00182 0.19 -1.3 195,566 - CCNA_00182 0.20 -2.6 195,566 - CCNA_00182 0.20 -1.7 195,566 - CCNA_00182 0.20 -1.7 195,566 - CCNA_00182 0.20 -1.9 195,573 + CCNA_00182 0.21 -2.8 195,573 + CCNA_00182 0.21 +0.3 195,573 + CCNA_00182 0.21 -1.4 195,581 - CCNA_00182 0.22 -1.4 195,631 - CCNA_00182 0.29 -1.0 195,636 + CCNA_00182 0.30 -0.2 195,640 - CCNA_00182 0.30 -1.7 195,654 + CCNA_00182 0.32 +0.7 195,672 - CCNA_00182 0.35 -1.2 195,683 - CCNA_00182 0.36 +0.3 195,696 + CCNA_00182 0.38 -1.8 195,711 - CCNA_00182 0.40 -0.7 195,757 + CCNA_00182 0.46 -0.6 195,757 + CCNA_00182 0.46 +1.3 195,757 + CCNA_00182 0.46 -0.2 195,758 + CCNA_00182 0.47 -2.5 195,758 + CCNA_00182 0.47 -1.2 195,758 + CCNA_00182 0.47 -2.1 195,758 + CCNA_00182 0.47 +1.2 195,765 - CCNA_00182 0.48 -1.8 195,765 - CCNA_00182 0.48 +1.2 195,765 - CCNA_00182 0.48 -1.0 195,765 - CCNA_00182 0.48 -2.8 195,765 - CCNA_00182 0.48 +0.2 195,765 - CCNA_00182 0.48 -1.2 195,765 - CCNA_00182 0.48 +0.1 195,825 + CCNA_00182 0.56 +0.4 195,825 + CCNA_00182 0.56 +1.9 195,882 + CCNA_00182 0.64 -2.1 195,892 + CCNA_00182 0.65 -0.5 195,892 + CCNA_00182 0.65 +1.1 195,914 - CCNA_00182 0.68 -0.5 195,916 + CCNA_00182 0.69 +0.2 195,937 - CCNA_00182 0.72 -2.0 195,985 - CCNA_00182 0.78 -0.4 195,985 - CCNA_00182 0.78 -0.5 195,995 - CCNA_00182 0.80 -0.2 195,996 + CCNA_00182 0.80 +0.8 196,004 - CCNA_00182 0.81 -1.5 196,028 - CCNA_00182 0.84 -1.3 196,046 + CCNA_00182 0.87 +0.6 196,051 + CCNA_00182 0.87 +0.5 196,059 - CCNA_00182 0.89 +1.8 196,114 + +0.7 196,246 + -1.4 196,259 - -1.0 196,261 - -1.9 196,275 + +0.4 196,275 + -1.1 196,275 + -0.6 196,278 - -0.6 196,283 - -1.3 196,361 + CCNA_00183 0.15 -0.1 196,361 + CCNA_00183 0.15 -0.5 196,362 - CCNA_00183 0.15 +0.8 196,369 - CCNA_00183 0.16 -0.5 196,369 - CCNA_00183 0.16 +0.3 196,403 + CCNA_00183 0.20 -0.2 196,406 - CCNA_00183 0.20 +0.8 196,406 - CCNA_00183 0.20 +0.5 196,408 + CCNA_00183 0.21 +1.4 196,462 - CCNA_00183 0.28 +1.2 196,478 - CCNA_00183 0.30 -0.9 196,500 + CCNA_00183 0.33 -1.1 196,500 + CCNA_00183 0.33 -0.1 196,500 + CCNA_00183 0.33 -1.0 196,511 - CCNA_00183 0.34 +0.6 196,530 - CCNA_00183 0.37 -0.5 196,553 + CCNA_00183 0.40 -0.1 196,553 + CCNA_00183 0.40 +1.3 196,553 + CCNA_00183 0.40 -2.8 196,576 - CCNA_00183 0.43 -0.6 196,619 + CCNA_00183 0.48 -2.9 196,619 + CCNA_00183 0.48 -0.2 196,632 - CCNA_00183 0.50 -0.6 196,656 + CCNA_00183 0.53 +0.6 196,699 - CCNA_00183 0.59 -0.7 196,772 - CCNA_00183 0.69 +0.6 196,776 + CCNA_00183 0.69 +1.6 196,779 - CCNA_00183 0.69 -0.8 196,784 - CCNA_00183 0.70 +1.3 196,814 - CCNA_00183 0.74 -1.3 196,814 - CCNA_00183 0.74 -0.7 196,833 - CCNA_00183 0.77 +0.2 196,851 + CCNA_00183 0.79 +0.0 196,868 - CCNA_00183 0.81 +0.9 196,901 - CCNA_00183 0.85 -2.1 196,913 - CCNA_00183 0.87 -1.8 196,989 + -0.9 196,989 + +0.3 196,997 - +0.3 196,997 - +1.1 196,997 - -0.2 196,997 - +0.2 196,997 - -0.7 197,014 - +0.0 197,015 - +0.8 197,017 + -0.1 197,027 + +0.5 197,027 + -0.1 197,035 - +0.2 197,043 + -0.5 197,043 + -0.3 197,051 - -0.9 197,051 - -0.8 197,099 + CCNA_00184 0.15 -0.4 197,119 - CCNA_00184 0.25 -1.6 197,121 + CCNA_00184 0.26 +1.6 197,129 - CCNA_00184 0.30 -2.3
Or see this region's nucleotide sequence