Experiment: Cisplatin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplS and tRNA-Trp are separated by 45 nucleotides tRNA-Trp and tRNA-Asp are separated by 206 nucleotides tRNA-Asp and petJ are separated by 213 nucleotides petJ and Synpcc7942_2543 are separated by 16 nucleotides Synpcc7942_2543 and Synpcc7942_2544 are separated by 29 nucleotides Synpcc7942_2544 and aspC are separated by 113 nucleotides
Synpcc7942_2541: rplS - 50S ribosomal protein L19, at 2,622,353 to 2,622,766
rplS
Synpcc7942_R0019: tRNA-Trp - tRNA-Trp, at 2,622,812 to 2,622,884
tRNA-Trp
Synpcc7942_R0020: tRNA-Asp - tRNA-Asp, at 2,623,091 to 2,623,164
tRNA-Asp
Synpcc7942_2542: petJ - putative cytochrome C6-2, at 2,623,378 to 2,623,725
petJ
Synpcc7942_2543: Synpcc7942_2543 - phage SPO1 DNA polymerase-related protein, at 2,623,742 to 2,624,449
_2543
Synpcc7942_2544: Synpcc7942_2544 - hypothetical protein, at 2,624,479 to 2,624,868
_2544
Synpcc7942_2545: aspC - aspartate aminotransferase, at 2,624,982 to 2,626,160
aspC
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2622.932 kb on + strand at 2622.943 kb on - strand at 2622.958 kb on - strand at 2623.200 kb on + strand at 2623.200 kb on + strand at 2623.227 kb on + strand at 2623.232 kb on + strand at 2623.235 kb on - strand at 2623.240 kb on - strand at 2623.265 kb on + strand at 2623.265 kb on + strand at 2623.267 kb on + strand at 2623.267 kb on + strand at 2623.307 kb on + strand at 2623.307 kb on + strand at 2623.343 kb on - strand at 2623.361 kb on - strand at 2623.376 kb on + strand at 2623.387 kb on + strand at 2623.405 kb on - strand at 2623.445 kb on + strand, within petJ at 2623.445 kb on + strand, within petJ at 2623.448 kb on - strand, within petJ at 2623.453 kb on - strand, within petJ at 2623.453 kb on - strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.507 kb on + strand, within petJ at 2623.509 kb on + strand, within petJ at 2623.509 kb on + strand, within petJ at 2623.515 kb on - strand, within petJ at 2623.517 kb on - strand, within petJ at 2623.517 kb on - strand, within petJ at 2623.527 kb on - strand, within petJ at 2623.541 kb on + strand, within petJ at 2623.569 kb on + strand, within petJ at 2623.598 kb on + strand, within petJ at 2623.610 kb on + strand, within petJ at 2623.610 kb on + strand at 2623.611 kb on - strand, within petJ at 2623.615 kb on + strand, within petJ at 2623.618 kb on - strand, within petJ at 2623.625 kb on + strand at 2623.633 kb on + strand, within petJ at 2623.671 kb on + strand, within petJ at 2623.694 kb on + strand at 2623.694 kb on - strand at 2623.702 kb on - strand at 2623.714 kb on - strand at 2623.720 kb on + strand at 2623.720 kb on + strand at 2623.720 kb on + strand at 2623.728 kb on - strand at 2623.728 kb on - strand at 2623.728 kb on - strand at 2623.730 kb on + strand at 2623.730 kb on + strand at 2623.738 kb on - strand at 2623.740 kb on + strand at 2623.740 kb on + strand at 2623.747 kb on - strand at 2623.748 kb on - strand at 2623.748 kb on - strand at 2623.822 kb on + strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.825 kb on - strand, within Synpcc7942_2543 at 2623.845 kb on + strand, within Synpcc7942_2543 at 2623.848 kb on - strand, within Synpcc7942_2543 at 2623.917 kb on + strand, within Synpcc7942_2543 at 2623.925 kb on + strand, within Synpcc7942_2543 at 2623.948 kb on - strand, within Synpcc7942_2543 at 2623.948 kb on - strand, within Synpcc7942_2543 at 2623.984 kb on + strand, within Synpcc7942_2543 at 2623.984 kb on + strand, within Synpcc7942_2543 at 2623.989 kb on - strand, within Synpcc7942_2543 at 2624.043 kb on + strand, within Synpcc7942_2543 at 2624.125 kb on + strand, within Synpcc7942_2543 at 2624.146 kb on - strand, within Synpcc7942_2543 at 2624.171 kb on + strand, within Synpcc7942_2543 at 2624.191 kb on - strand, within Synpcc7942_2543 at 2624.196 kb on - strand, within Synpcc7942_2543 at 2624.224 kb on + strand, within Synpcc7942_2543 at 2624.235 kb on + strand, within Synpcc7942_2543 at 2624.245 kb on + strand, within Synpcc7942_2543 at 2624.247 kb on - strand, within Synpcc7942_2543 at 2624.271 kb on + strand, within Synpcc7942_2543 at 2624.276 kb on + strand, within Synpcc7942_2543 at 2624.286 kb on + strand, within Synpcc7942_2543 at 2624.287 kb on + strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.294 kb on - strand, within Synpcc7942_2543 at 2624.296 kb on + strand, within Synpcc7942_2543 at 2624.325 kb on - strand, within Synpcc7942_2543 at 2624.346 kb on - strand, within Synpcc7942_2543 at 2624.354 kb on + strand, within Synpcc7942_2543 at 2624.388 kb on - strand at 2624.428 kb on + strand at 2624.428 kb on + strand at 2624.428 kb on + strand at 2624.432 kb on + strand at 2624.451 kb on + strand at 2624.476 kb on + strand at 2624.484 kb on - strand at 2624.484 kb on - strand at 2624.484 kb on - strand at 2624.486 kb on + strand at 2624.494 kb on - strand at 2624.517 kb on + strand at 2624.527 kb on - strand, within Synpcc7942_2544 at 2624.535 kb on - strand, within Synpcc7942_2544 at 2624.572 kb on + strand, within Synpcc7942_2544 at 2624.579 kb on + strand, within Synpcc7942_2544 at 2624.597 kb on + strand, within Synpcc7942_2544 at 2624.617 kb on + strand, within Synpcc7942_2544 at 2624.625 kb on - strand, within Synpcc7942_2544 at 2624.661 kb on + strand, within Synpcc7942_2544 at 2624.661 kb on + strand, within Synpcc7942_2544 at 2624.669 kb on + strand, within Synpcc7942_2544 at 2624.706 kb on + strand, within Synpcc7942_2544 at 2624.706 kb on + strand, within Synpcc7942_2544 at 2624.714 kb on - strand, within Synpcc7942_2544 at 2624.771 kb on + strand, within Synpcc7942_2544 at 2624.771 kb on + strand, within Synpcc7942_2544 at 2624.771 kb on + strand, within Synpcc7942_2544 at 2624.779 kb on - strand, within Synpcc7942_2544 at 2624.779 kb on - strand, within Synpcc7942_2544 at 2624.797 kb on + strand, within Synpcc7942_2544 at 2624.802 kb on + strand, within Synpcc7942_2544 at 2624.802 kb on - strand, within Synpcc7942_2544 at 2624.804 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on + strand, within Synpcc7942_2544 at 2624.807 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.812 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.815 kb on - strand, within Synpcc7942_2544 at 2624.843 kb on + strand at 2624.853 kb on + strand at 2624.853 kb on + strand at 2624.853 kb on + strand at 2624.861 kb on - strand at 2624.879 kb on + strand at 2624.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.00625 mg/ml remove 2,622,932 + +0.8 2,622,943 - -0.2 2,622,958 - -1.2 2,623,200 + -1.8 2,623,200 + -3.2 2,623,227 + +4.4 2,623,232 + +0.4 2,623,235 - +3.2 2,623,240 - -0.2 2,623,265 + -0.2 2,623,265 + -1.2 2,623,267 + -0.2 2,623,267 + +0.6 2,623,307 + -1.8 2,623,307 + +2.7 2,623,343 - -1.2 2,623,361 - +2.8 2,623,376 + +0.7 2,623,387 + -0.2 2,623,405 - -2.2 2,623,445 + petJ Synpcc7942_2542 0.19 -2.2 2,623,445 + petJ Synpcc7942_2542 0.19 +3.7 2,623,448 - petJ Synpcc7942_2542 0.20 +2.5 2,623,453 - petJ Synpcc7942_2542 0.22 +0.1 2,623,453 - petJ Synpcc7942_2542 0.22 -0.2 2,623,507 + petJ Synpcc7942_2542 0.37 +2.4 2,623,507 + petJ Synpcc7942_2542 0.37 +4.9 2,623,507 + petJ Synpcc7942_2542 0.37 +4.4 2,623,507 + petJ Synpcc7942_2542 0.37 +1.9 2,623,507 + petJ Synpcc7942_2542 0.37 -2.5 2,623,509 + petJ Synpcc7942_2542 0.38 -2.5 2,623,509 + petJ Synpcc7942_2542 0.38 +1.8 2,623,515 - petJ Synpcc7942_2542 0.39 +2.4 2,623,517 - petJ Synpcc7942_2542 0.40 +0.8 2,623,517 - petJ Synpcc7942_2542 0.40 -1.8 2,623,527 - petJ Synpcc7942_2542 0.43 -0.2 2,623,541 + petJ Synpcc7942_2542 0.47 +4.1 2,623,569 + petJ Synpcc7942_2542 0.55 +2.4 2,623,598 + petJ Synpcc7942_2542 0.63 -1.2 2,623,610 + petJ Synpcc7942_2542 0.67 +2.1 2,623,610 + -0.2 2,623,611 - petJ Synpcc7942_2542 0.67 -1.2 2,623,615 + petJ Synpcc7942_2542 0.68 -1.8 2,623,618 - petJ Synpcc7942_2542 0.69 -0.2 2,623,625 + -1.2 2,623,633 + petJ Synpcc7942_2542 0.73 +2.2 2,623,671 + petJ Synpcc7942_2542 0.84 -1.8 2,623,694 + +1.7 2,623,694 - -1.4 2,623,702 - -0.2 2,623,714 - -1.2 2,623,720 + +5.8 2,623,720 + +0.3 2,623,720 + +3.6 2,623,728 - +1.8 2,623,728 - -1.2 2,623,728 - -0.2 2,623,730 + +2.8 2,623,730 + +0.6 2,623,738 - -2.2 2,623,740 + +5.6 2,623,740 + -2.5 2,623,747 - -1.2 2,623,748 - +2.7 2,623,748 - +1.1 2,623,822 + Synpcc7942_2543 0.11 +2.8 2,623,825 - Synpcc7942_2543 0.12 +2.1 2,623,825 - Synpcc7942_2543 0.12 -0.2 2,623,825 - Synpcc7942_2543 0.12 +2.8 2,623,825 - Synpcc7942_2543 0.12 -1.8 2,623,825 - Synpcc7942_2543 0.12 +3.5 2,623,845 + Synpcc7942_2543 0.15 -0.2 2,623,848 - Synpcc7942_2543 0.15 +3.4 2,623,917 + Synpcc7942_2543 0.25 -1.2 2,623,925 + Synpcc7942_2543 0.26 +4.2 2,623,948 - Synpcc7942_2543 0.29 +2.1 2,623,948 - Synpcc7942_2543 0.29 +4.7 2,623,984 + Synpcc7942_2543 0.34 -1.8 2,623,984 + Synpcc7942_2543 0.34 +5.5 2,623,989 - Synpcc7942_2543 0.35 +3.2 2,624,043 + Synpcc7942_2543 0.43 +1.4 2,624,125 + Synpcc7942_2543 0.54 +4.3 2,624,146 - Synpcc7942_2543 0.57 +3.6 2,624,171 + Synpcc7942_2543 0.61 +0.8 2,624,191 - Synpcc7942_2543 0.63 -1.9 2,624,196 - Synpcc7942_2543 0.64 +1.3 2,624,224 + Synpcc7942_2543 0.68 +3.1 2,624,235 + Synpcc7942_2543 0.70 +2.2 2,624,245 + Synpcc7942_2543 0.71 +3.2 2,624,247 - Synpcc7942_2543 0.71 +5.6 2,624,271 + Synpcc7942_2543 0.75 +3.1 2,624,276 + Synpcc7942_2543 0.75 -1.2 2,624,286 + Synpcc7942_2543 0.77 +1.7 2,624,287 + Synpcc7942_2543 0.77 -0.2 2,624,294 - Synpcc7942_2543 0.78 -1.2 2,624,294 - Synpcc7942_2543 0.78 -0.9 2,624,294 - Synpcc7942_2543 0.78 +2.3 2,624,294 - Synpcc7942_2543 0.78 +5.3 2,624,294 - Synpcc7942_2543 0.78 -1.2 2,624,296 + Synpcc7942_2543 0.78 +4.0 2,624,325 - Synpcc7942_2543 0.82 +3.0 2,624,346 - Synpcc7942_2543 0.85 +0.8 2,624,354 + Synpcc7942_2543 0.86 -0.2 2,624,388 - -0.2 2,624,428 + -0.2 2,624,428 + +0.8 2,624,428 + -1.8 2,624,432 + -0.2 2,624,451 + -1.8 2,624,476 + +2.0 2,624,484 - +5.1 2,624,484 - +0.8 2,624,484 - -0.2 2,624,486 + +4.2 2,624,494 - +2.6 2,624,517 + -1.3 2,624,527 - Synpcc7942_2544 0.12 +2.5 2,624,535 - Synpcc7942_2544 0.14 +3.1 2,624,572 + Synpcc7942_2544 0.24 -0.2 2,624,579 + Synpcc7942_2544 0.26 +4.1 2,624,597 + Synpcc7942_2544 0.30 -0.2 2,624,617 + Synpcc7942_2544 0.35 +0.1 2,624,625 - Synpcc7942_2544 0.37 +0.8 2,624,661 + Synpcc7942_2544 0.47 -0.2 2,624,661 + Synpcc7942_2544 0.47 +3.1 2,624,669 + Synpcc7942_2544 0.49 +2.4 2,624,706 + Synpcc7942_2544 0.58 +6.0 2,624,706 + Synpcc7942_2544 0.58 -1.2 2,624,714 - Synpcc7942_2544 0.60 +2.4 2,624,771 + Synpcc7942_2544 0.75 -1.2 2,624,771 + Synpcc7942_2544 0.75 +2.0 2,624,771 + Synpcc7942_2544 0.75 +4.4 2,624,779 - Synpcc7942_2544 0.77 -1.2 2,624,779 - Synpcc7942_2544 0.77 -1.8 2,624,797 + Synpcc7942_2544 0.82 +2.2 2,624,802 + Synpcc7942_2544 0.83 +2.2 2,624,802 - Synpcc7942_2544 0.83 -1.8 2,624,804 + Synpcc7942_2544 0.83 -0.8 2,624,807 + Synpcc7942_2544 0.84 +1.4 2,624,807 + Synpcc7942_2544 0.84 -2.2 2,624,807 + Synpcc7942_2544 0.84 +4.0 2,624,807 - Synpcc7942_2544 0.84 +1.7 2,624,812 - Synpcc7942_2544 0.85 +3.6 2,624,812 - Synpcc7942_2544 0.85 +4.3 2,624,812 - Synpcc7942_2544 0.85 +3.1 2,624,812 - Synpcc7942_2544 0.85 -0.2 2,624,812 - Synpcc7942_2544 0.85 +2.7 2,624,815 - Synpcc7942_2544 0.86 -2.8 2,624,815 - Synpcc7942_2544 0.86 +2.6 2,624,815 - Synpcc7942_2544 0.86 -0.2 2,624,815 - Synpcc7942_2544 0.86 +0.4 2,624,815 - Synpcc7942_2544 0.86 +0.5 2,624,815 - Synpcc7942_2544 0.86 +1.0 2,624,843 + -3.3 2,624,853 + +2.3 2,624,853 + +5.7 2,624,853 + +0.8 2,624,861 - -2.8 2,624,879 + +2.7 2,624,920 - +1.6
Or see this region's nucleotide sequence