Experiment: Cisplatin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpPIII and clpR are separated by 42 nucleotides clpR and Synpcc7942_2539 are separated by 141 nucleotides Synpcc7942_2539 and digB are separated by 189 nucleotides digB and rplS are separated by 54 nucleotides
Synpcc7942_2537: clpPIII - ATP-dependent Clp protease proteolytic subunit, at 2,619,632 to 2,620,231
clpPIII
Synpcc7942_2538: clpR - ATP-dependent Clp protease-like protein, at 2,620,274 to 2,620,960
clpR
Synpcc7942_2539: Synpcc7942_2539 - hypothetical protein, at 2,621,102 to 2,621,647
_2539
Synpcc7942_2540: digB - blue-copper-protein-like protein, at 2,621,837 to 2,622,298
digB
Synpcc7942_2541: rplS - 50S ribosomal protein L19, at 2,622,353 to 2,622,766
rplS
Position (kb)
2621
2622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2620.994 kb on + strand at 2621.002 kb on - strand at 2621.018 kb on + strand at 2621.033 kb on + strand at 2621.034 kb on + strand at 2621.034 kb on + strand at 2621.034 kb on + strand at 2621.042 kb on - strand at 2621.042 kb on - strand at 2621.062 kb on + strand at 2621.065 kb on + strand at 2621.083 kb on + strand at 2621.088 kb on + strand at 2621.089 kb on + strand at 2621.091 kb on - strand at 2621.091 kb on - strand at 2621.091 kb on - strand at 2621.095 kb on + strand at 2621.095 kb on + strand at 2621.095 kb on + strand at 2621.095 kb on + strand at 2621.097 kb on + strand at 2621.103 kb on - strand at 2621.103 kb on - strand at 2621.103 kb on - strand at 2621.151 kb on + strand at 2621.185 kb on - strand, within Synpcc7942_2539 at 2621.185 kb on - strand, within Synpcc7942_2539 at 2621.195 kb on + strand, within Synpcc7942_2539 at 2621.682 kb on + strand at 2621.690 kb on - strand at 2621.690 kb on - strand at 2621.705 kb on + strand at 2621.710 kb on + strand at 2621.720 kb on + strand at 2621.720 kb on + strand at 2621.728 kb on - strand at 2621.734 kb on + strand at 2621.735 kb on - strand at 2621.742 kb on - strand at 2621.742 kb on - strand at 2621.755 kb on - strand at 2621.755 kb on - strand at 2621.772 kb on - strand at 2621.772 kb on - strand at 2621.772 kb on - strand at 2621.800 kb on + strand at 2621.800 kb on + strand at 2621.818 kb on - strand at 2621.818 kb on - strand at 2621.818 kb on - strand at 2621.852 kb on - strand at 2621.852 kb on - strand at 2621.857 kb on - strand at 2621.859 kb on + strand at 2621.859 kb on + strand at 2621.867 kb on - strand at 2621.878 kb on + strand at 2621.899 kb on + strand, within digB at 2621.938 kb on + strand, within digB at 2621.938 kb on + strand, within digB at 2621.946 kb on + strand, within digB at 2621.946 kb on - strand, within digB at 2621.986 kb on - strand at 2621.999 kb on + strand, within digB at 2622.043 kb on - strand, within digB at 2622.053 kb on - strand, within digB at 2622.061 kb on - strand, within digB at 2622.061 kb on - strand, within digB at 2622.069 kb on + strand, within digB at 2622.074 kb on + strand, within digB at 2622.082 kb on - strand, within digB at 2622.082 kb on - strand, within digB at 2622.087 kb on + strand, within digB at 2622.097 kb on + strand, within digB at 2622.164 kb on + strand, within digB at 2622.175 kb on + strand, within digB at 2622.175 kb on + strand, within digB at 2622.202 kb on - strand, within digB at 2622.215 kb on - strand, within digB at 2622.220 kb on - strand, within digB at 2622.222 kb on + strand, within digB at 2622.230 kb on - strand, within digB at 2622.230 kb on - strand, within digB at 2622.230 kb on - strand, within digB at 2622.250 kb on - strand, within digB at 2622.257 kb on + strand at 2622.265 kb on - strand at 2622.280 kb on + strand at 2622.282 kb on + strand at 2622.282 kb on + strand at 2622.282 kb on + strand at 2622.282 kb on + strand at 2622.282 kb on + strand at 2622.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.00625 mg/ml remove 2,620,994 + +3.1 2,621,002 - +4.3 2,621,018 + +3.5 2,621,033 + -1.9 2,621,034 + -0.2 2,621,034 + +1.5 2,621,034 + -0.2 2,621,042 - -2.5 2,621,042 - -2.2 2,621,062 + +2.2 2,621,065 + -1.0 2,621,083 + -1.2 2,621,088 + +4.0 2,621,089 + +3.4 2,621,091 - -2.8 2,621,091 - -0.2 2,621,091 - +0.6 2,621,095 + -0.2 2,621,095 + -1.2 2,621,095 + -1.8 2,621,095 + -1.2 2,621,097 + +1.6 2,621,103 - -2.2 2,621,103 - -0.6 2,621,103 - +2.6 2,621,151 + -0.2 2,621,185 - Synpcc7942_2539 0.15 +0.1 2,621,185 - Synpcc7942_2539 0.15 -0.2 2,621,195 + Synpcc7942_2539 0.17 -1.8 2,621,682 + +2.1 2,621,690 - -1.5 2,621,690 - -1.2 2,621,705 + -2.2 2,621,710 + +1.8 2,621,720 + -2.0 2,621,720 + +3.1 2,621,728 - -3.0 2,621,734 + -2.2 2,621,735 - -0.2 2,621,742 - -1.2 2,621,742 - -3.2 2,621,755 - +2.8 2,621,755 - +1.3 2,621,772 - -2.2 2,621,772 - -0.2 2,621,772 - -0.2 2,621,800 + -2.8 2,621,800 + -0.2 2,621,818 - -1.2 2,621,818 - +3.6 2,621,818 - -2.5 2,621,852 - +3.7 2,621,852 - +1.9 2,621,857 - -1.8 2,621,859 + +2.8 2,621,859 + +3.5 2,621,867 - -1.8 2,621,878 + +0.2 2,621,899 + digB Synpcc7942_2540 0.13 -2.2 2,621,938 + digB Synpcc7942_2540 0.22 +0.4 2,621,938 + digB Synpcc7942_2540 0.22 +2.8 2,621,946 + digB Synpcc7942_2540 0.24 +1.7 2,621,946 - digB Synpcc7942_2540 0.24 -1.2 2,621,986 - -1.2 2,621,999 + digB Synpcc7942_2540 0.35 +3.8 2,622,043 - digB Synpcc7942_2540 0.45 -0.3 2,622,053 - digB Synpcc7942_2540 0.47 -0.2 2,622,061 - digB Synpcc7942_2540 0.48 -1.8 2,622,061 - digB Synpcc7942_2540 0.48 +2.1 2,622,069 + digB Synpcc7942_2540 0.50 -2.0 2,622,074 + digB Synpcc7942_2540 0.51 -2.8 2,622,082 - digB Synpcc7942_2540 0.53 -1.2 2,622,082 - digB Synpcc7942_2540 0.53 -0.9 2,622,087 + digB Synpcc7942_2540 0.54 -1.8 2,622,097 + digB Synpcc7942_2540 0.56 -1.2 2,622,164 + digB Synpcc7942_2540 0.71 -0.2 2,622,175 + digB Synpcc7942_2540 0.73 -0.2 2,622,175 + digB Synpcc7942_2540 0.73 +1.4 2,622,202 - digB Synpcc7942_2540 0.79 -0.2 2,622,215 - digB Synpcc7942_2540 0.82 -0.8 2,622,220 - digB Synpcc7942_2540 0.83 -0.2 2,622,222 + digB Synpcc7942_2540 0.83 -3.9 2,622,230 - digB Synpcc7942_2540 0.85 +1.8 2,622,230 - digB Synpcc7942_2540 0.85 +2.2 2,622,230 - digB Synpcc7942_2540 0.85 +1.9 2,622,250 - digB Synpcc7942_2540 0.89 +1.4 2,622,257 + -1.8 2,622,265 - +1.8 2,622,280 + +2.7 2,622,282 + -1.2 2,622,282 + -0.2 2,622,282 + +1.9 2,622,282 + +4.9 2,622,282 + -0.2 2,622,290 - +0.8
Or see this region's nucleotide sequence