Experiment: Cisplatin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0068 and Synpcc7942_0069 are separated by 98 nucleotides Synpcc7942_0069 and Synpcc7942_0070 are separated by 2 nucleotides Synpcc7942_0070 and degT are separated by 1 nucleotides degT and Synpcc7942_0072 are separated by 167 nucleotides Synpcc7942_0072 and lytB are separated by 39 nucleotides
Synpcc7942_0068: Synpcc7942_0068 - ATPase, at 67,991 to 68,980
_0068
Synpcc7942_0069: Synpcc7942_0069 - hypothetical protein, at 69,079 to 69,375
_0069
Synpcc7942_0070: Synpcc7942_0070 - hypothetical protein, at 69,378 to 69,938
_0070
Synpcc7942_0071: degT - pleiotropic regulatory protein-like, at 69,940 to 71,139
degT
Synpcc7942_0072: Synpcc7942_0072 - hypothetical protein, at 71,307 to 71,960
_0072
Synpcc7942_0073: lytB - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 72,000 to 73,196
lytB
Position (kb)
69
70
71
72 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 68.952 kb on - strand at 68.972 kb on + strand at 68.980 kb on - strand at 68.985 kb on + strand at 68.985 kb on - strand at 68.991 kb on + strand at 68.996 kb on + strand at 68.996 kb on + strand at 68.996 kb on + strand at 69.001 kb on + strand at 69.001 kb on + strand at 69.004 kb on - strand at 69.004 kb on - strand at 69.009 kb on - strand at 69.009 kb on - strand at 69.014 kb on - strand at 69.016 kb on + strand at 69.016 kb on - strand at 69.080 kb on - strand at 69.080 kb on - strand at 69.160 kb on + strand, within Synpcc7942_0069 at 69.160 kb on + strand, within Synpcc7942_0069 at 69.168 kb on - strand, within Synpcc7942_0069 at 69.173 kb on - strand, within Synpcc7942_0069 at 69.179 kb on - strand, within Synpcc7942_0069 at 69.214 kb on + strand, within Synpcc7942_0069 at 69.214 kb on + strand, within Synpcc7942_0069 at 69.217 kb on + strand at 69.230 kb on - strand, within Synpcc7942_0069 at 69.235 kb on - strand, within Synpcc7942_0069 at 69.253 kb on + strand, within Synpcc7942_0069 at 69.261 kb on - strand, within Synpcc7942_0069 at 69.287 kb on + strand, within Synpcc7942_0069 at 69.287 kb on + strand, within Synpcc7942_0069 at 69.287 kb on + strand, within Synpcc7942_0069 at 69.289 kb on + strand, within Synpcc7942_0069 at 69.292 kb on + strand, within Synpcc7942_0069 at 69.297 kb on + strand, within Synpcc7942_0069 at 69.297 kb on + strand, within Synpcc7942_0069 at 69.297 kb on + strand, within Synpcc7942_0069 at 69.297 kb on + strand, within Synpcc7942_0069 at 69.300 kb on - strand, within Synpcc7942_0069 at 69.305 kb on - strand, within Synpcc7942_0069 at 69.307 kb on - strand, within Synpcc7942_0069 at 69.347 kb on - strand at 69.370 kb on - strand at 69.377 kb on - strand at 69.422 kb on + strand at 69.422 kb on + strand at 69.430 kb on - strand at 69.502 kb on - strand, within Synpcc7942_0070 at 69.502 kb on - strand, within Synpcc7942_0070 at 69.515 kb on + strand, within Synpcc7942_0070 at 69.515 kb on + strand, within Synpcc7942_0070 at 69.551 kb on + strand, within Synpcc7942_0070 at 69.552 kb on + strand, within Synpcc7942_0070 at 69.560 kb on - strand, within Synpcc7942_0070 at 69.562 kb on + strand, within Synpcc7942_0070 at 69.562 kb on + strand, within Synpcc7942_0070 at 69.562 kb on + strand, within Synpcc7942_0070 at 69.562 kb on + strand, within Synpcc7942_0070 at 69.562 kb on + strand, within Synpcc7942_0070 at 69.562 kb on + strand, within Synpcc7942_0070 at 69.570 kb on - strand, within Synpcc7942_0070 at 69.570 kb on - strand, within Synpcc7942_0070 at 69.570 kb on - strand, within Synpcc7942_0070 at 69.570 kb on - strand, within Synpcc7942_0070 at 69.570 kb on - strand, within Synpcc7942_0070 at 69.570 kb on - strand, within Synpcc7942_0070 at 69.574 kb on + strand, within Synpcc7942_0070 at 69.577 kb on + strand, within Synpcc7942_0070 at 69.577 kb on - strand, within Synpcc7942_0070 at 69.582 kb on + strand, within Synpcc7942_0070 at 69.582 kb on - strand, within Synpcc7942_0070 at 69.585 kb on - strand at 69.645 kb on + strand, within Synpcc7942_0070 at 69.710 kb on - strand, within Synpcc7942_0070 at 69.739 kb on + strand, within Synpcc7942_0070 at 69.779 kb on - strand, within Synpcc7942_0070 at 69.845 kb on - strand, within Synpcc7942_0070 at 69.909 kb on + strand at 69.909 kb on + strand at 69.909 kb on + strand at 69.909 kb on + strand at 69.917 kb on - strand at 69.917 kb on - strand at 69.917 kb on - strand at 69.929 kb on + strand at 69.934 kb on + strand at 69.946 kb on - strand at 71.112 kb on + strand at 71.134 kb on + strand at 71.146 kb on - strand at 71.159 kb on - strand at 71.160 kb on - strand at 71.171 kb on + strand at 71.171 kb on + strand at 71.171 kb on + strand at 71.171 kb on + strand at 71.171 kb on + strand at 71.171 kb on + strand at 71.179 kb on - strand at 71.179 kb on - strand at 71.179 kb on - strand at 71.242 kb on - strand at 71.262 kb on + strand at 71.288 kb on + strand at 71.294 kb on - strand at 71.296 kb on - strand at 71.378 kb on + strand, within Synpcc7942_0072 at 71.386 kb on - strand, within Synpcc7942_0072 at 71.396 kb on - strand, within Synpcc7942_0072 at 71.396 kb on - strand, within Synpcc7942_0072 at 71.431 kb on + strand, within Synpcc7942_0072 at 71.431 kb on + strand, within Synpcc7942_0072 at 71.480 kb on - strand, within Synpcc7942_0072 at 71.485 kb on - strand, within Synpcc7942_0072 at 71.499 kb on - strand, within Synpcc7942_0072 at 71.503 kb on + strand, within Synpcc7942_0072 at 71.511 kb on - strand, within Synpcc7942_0072 at 71.571 kb on - strand, within Synpcc7942_0072 at 71.576 kb on - strand, within Synpcc7942_0072 at 71.604 kb on - strand, within Synpcc7942_0072 at 71.610 kb on + strand, within Synpcc7942_0072 at 71.629 kb on - strand, within Synpcc7942_0072 at 71.634 kb on - strand, within Synpcc7942_0072 at 71.634 kb on - strand, within Synpcc7942_0072 at 71.653 kb on - strand, within Synpcc7942_0072 at 71.661 kb on - strand at 71.694 kb on + strand, within Synpcc7942_0072 at 71.694 kb on + strand, within Synpcc7942_0072 at 71.701 kb on + strand, within Synpcc7942_0072 at 71.709 kb on - strand, within Synpcc7942_0072 at 71.742 kb on - strand, within Synpcc7942_0072 at 71.772 kb on + strand, within Synpcc7942_0072 at 71.817 kb on - strand, within Synpcc7942_0072 at 71.824 kb on - strand, within Synpcc7942_0072 at 71.827 kb on - strand, within Synpcc7942_0072 at 71.834 kb on + strand, within Synpcc7942_0072 at 71.881 kb on - strand, within Synpcc7942_0072 at 71.892 kb on - strand, within Synpcc7942_0072 at 71.903 kb on + strand at 71.911 kb on - strand at 71.918 kb on - strand at 71.918 kb on - strand at 71.942 kb on - strand at 71.962 kb on - strand at 71.966 kb on - strand at 71.972 kb on - strand at 71.974 kb on - strand at 71.998 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.00625 mg/ml remove 68,952 - -0.2 68,972 + -1.2 68,980 - +4.3 68,985 + -3.1 68,985 - +4.0 68,991 + -0.2 68,996 + -1.2 68,996 + -1.2 68,996 + -0.2 69,001 + +3.4 69,001 + -1.8 69,004 - -3.0 69,004 - -0.2 69,009 - +0.5 69,009 - +3.2 69,014 - -3.0 69,016 + +1.9 69,016 - +3.3 69,080 - +0.3 69,080 - +0.9 69,160 + Synpcc7942_0069 0.27 -1.5 69,160 + Synpcc7942_0069 0.27 -0.2 69,168 - Synpcc7942_0069 0.30 -0.2 69,173 - Synpcc7942_0069 0.32 +1.3 69,179 - Synpcc7942_0069 0.34 -1.8 69,214 + Synpcc7942_0069 0.45 -2.2 69,214 + Synpcc7942_0069 0.45 -0.2 69,217 + -1.8 69,230 - Synpcc7942_0069 0.51 +2.6 69,235 - Synpcc7942_0069 0.53 -2.2 69,253 + Synpcc7942_0069 0.59 +2.1 69,261 - Synpcc7942_0069 0.61 -1.2 69,287 + Synpcc7942_0069 0.70 +2.0 69,287 + Synpcc7942_0069 0.70 +0.1 69,287 + Synpcc7942_0069 0.70 -1.8 69,289 + Synpcc7942_0069 0.71 +1.5 69,292 + Synpcc7942_0069 0.72 +0.2 69,297 + Synpcc7942_0069 0.73 -0.8 69,297 + Synpcc7942_0069 0.73 +0.5 69,297 + Synpcc7942_0069 0.73 -4.2 69,297 + Synpcc7942_0069 0.73 -0.2 69,300 - Synpcc7942_0069 0.74 -3.0 69,305 - Synpcc7942_0069 0.76 +1.3 69,307 - Synpcc7942_0069 0.77 +1.9 69,347 - -0.2 69,370 - +2.7 69,377 - -0.2 69,422 + -0.2 69,422 + +0.8 69,430 - +2.7 69,502 - Synpcc7942_0070 0.22 -3.0 69,502 - Synpcc7942_0070 0.22 -2.2 69,515 + Synpcc7942_0070 0.24 -1.2 69,515 + Synpcc7942_0070 0.24 +2.6 69,551 + Synpcc7942_0070 0.31 -0.2 69,552 + Synpcc7942_0070 0.31 +0.3 69,560 - Synpcc7942_0070 0.32 -0.8 69,562 + Synpcc7942_0070 0.33 +3.3 69,562 + Synpcc7942_0070 0.33 +1.5 69,562 + Synpcc7942_0070 0.33 +2.7 69,562 + Synpcc7942_0070 0.33 -0.2 69,562 + Synpcc7942_0070 0.33 -2.2 69,562 + Synpcc7942_0070 0.33 +2.1 69,570 - Synpcc7942_0070 0.34 +6.2 69,570 - Synpcc7942_0070 0.34 -0.2 69,570 - Synpcc7942_0070 0.34 +2.2 69,570 - Synpcc7942_0070 0.34 -1.2 69,570 - Synpcc7942_0070 0.34 -0.2 69,570 - Synpcc7942_0070 0.34 +4.4 69,574 + Synpcc7942_0070 0.35 +1.5 69,577 + Synpcc7942_0070 0.35 +5.3 69,577 - Synpcc7942_0070 0.35 +1.4 69,582 + Synpcc7942_0070 0.36 -1.8 69,582 - Synpcc7942_0070 0.36 -0.2 69,585 - +1.4 69,645 + Synpcc7942_0070 0.48 +3.3 69,710 - Synpcc7942_0070 0.59 +0.5 69,739 + Synpcc7942_0070 0.64 -2.2 69,779 - Synpcc7942_0070 0.71 -3.0 69,845 - Synpcc7942_0070 0.83 -0.2 69,909 + -1.8 69,909 + -0.2 69,909 + +4.3 69,909 + +2.7 69,917 - +3.7 69,917 - +3.0 69,917 - -1.2 69,929 + -1.2 69,934 + -1.2 69,946 - +2.5 71,112 + -0.2 71,134 + +0.8 71,146 - +2.0 71,159 - +0.8 71,160 - +0.4 71,171 + +2.9 71,171 + +3.3 71,171 + +2.2 71,171 + +0.8 71,171 + -3.5 71,171 + -0.2 71,179 - +1.5 71,179 - +4.3 71,179 - +2.7 71,242 - -1.8 71,262 + +3.7 71,288 + -1.5 71,294 - -1.2 71,296 - -2.0 71,378 + Synpcc7942_0072 0.11 +3.0 71,386 - Synpcc7942_0072 0.12 +6.5 71,396 - Synpcc7942_0072 0.14 -1.2 71,396 - Synpcc7942_0072 0.14 +3.4 71,431 + Synpcc7942_0072 0.19 +1.5 71,431 + Synpcc7942_0072 0.19 +2.4 71,480 - Synpcc7942_0072 0.26 -1.2 71,485 - Synpcc7942_0072 0.27 +3.0 71,499 - Synpcc7942_0072 0.29 -0.2 71,503 + Synpcc7942_0072 0.30 +1.7 71,511 - Synpcc7942_0072 0.31 +0.8 71,571 - Synpcc7942_0072 0.40 -1.8 71,576 - Synpcc7942_0072 0.41 -0.2 71,604 - Synpcc7942_0072 0.45 +2.4 71,610 + Synpcc7942_0072 0.46 +2.8 71,629 - Synpcc7942_0072 0.49 -2.5 71,634 - Synpcc7942_0072 0.50 -1.8 71,634 - Synpcc7942_0072 0.50 -0.2 71,653 - Synpcc7942_0072 0.53 -2.2 71,661 - -0.2 71,694 + Synpcc7942_0072 0.59 +1.2 71,694 + Synpcc7942_0072 0.59 +2.8 71,701 + Synpcc7942_0072 0.60 -2.8 71,709 - Synpcc7942_0072 0.61 -1.2 71,742 - Synpcc7942_0072 0.67 -1.8 71,772 + Synpcc7942_0072 0.71 +4.1 71,817 - Synpcc7942_0072 0.78 -0.2 71,824 - Synpcc7942_0072 0.79 +3.3 71,827 - Synpcc7942_0072 0.80 +2.3 71,834 + Synpcc7942_0072 0.81 +1.4 71,881 - Synpcc7942_0072 0.88 +1.1 71,892 - Synpcc7942_0072 0.89 +1.6 71,903 + -0.2 71,911 - +2.9 71,918 - -0.2 71,918 - +3.1 71,942 - -0.2 71,962 - -1.8 71,966 - +0.8 71,972 - -1.2 71,974 - -2.5 71,998 + +1.4
Or see this region's nucleotide sequence