Experiment: Gelatin (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0300 and SMa0301 overlap by 4 nucleotides SMa0301 and SMa0302 are separated by 69 nucleotides SMa0302 and SMa0303 are separated by 107 nucleotides
SMa0300: SMa0300 - ABC transporter permease, at 165,742 to 166,695
SMa0300
SMa0301: SMa0301 - hypothetical protein, at 166,692 to 167,360
SMa0301
SMa0302: SMa0302 - ABC transporter substrate-binding protein, at 167,430 to 168,956
SMa0302
SMa0303: SMa0303 - LysR family transcriptional regulator, at 169,064 to 169,942
SMa0303
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 166.496 kb on + strand, within SMa0300 at 166.685 kb on - strand at 166.685 kb on - strand at 166.685 kb on - strand at 166.685 kb on - strand at 166.789 kb on + strand, within SMa0301 at 166.789 kb on + strand, within SMa0301 at 166.789 kb on + strand, within SMa0301 at 166.790 kb on - strand, within SMa0301 at 166.790 kb on - strand, within SMa0301 at 166.790 kb on - strand, within SMa0301 at 166.925 kb on - strand, within SMa0301 at 166.927 kb on + strand, within SMa0301 at 167.000 kb on + strand, within SMa0301 at 167.001 kb on - strand, within SMa0301 at 167.034 kb on - strand, within SMa0301 at 167.056 kb on + strand, within SMa0301 at 167.057 kb on - strand, within SMa0301 at 167.101 kb on + strand, within SMa0301 at 167.101 kb on + strand, within SMa0301 at 167.102 kb on - strand, within SMa0301 at 167.366 kb on + strand at 167.366 kb on + strand at 167.367 kb on - strand at 167.456 kb on - strand at 167.456 kb on - strand at 167.488 kb on + strand at 167.546 kb on + strand at 167.734 kb on + strand, within SMa0302 at 167.734 kb on + strand, within SMa0302 at 167.735 kb on - strand, within SMa0302 at 167.776 kb on + strand, within SMa0302 at 167.839 kb on + strand, within SMa0302 at 167.884 kb on + strand, within SMa0302 at 167.884 kb on + strand, within SMa0302 at 168.123 kb on + strand, within SMa0302 at 168.124 kb on - strand, within SMa0302 at 168.133 kb on + strand, within SMa0302 at 168.134 kb on - strand, within SMa0302 at 168.263 kb on + strand, within SMa0302 at 168.303 kb on - strand, within SMa0302 at 168.362 kb on + strand, within SMa0302 at 168.363 kb on - strand, within SMa0302 at 168.363 kb on - strand, within SMa0302 at 168.399 kb on - strand, within SMa0302 at 168.451 kb on + strand, within SMa0302 at 168.451 kb on + strand, within SMa0302 at 168.451 kb on + strand, within SMa0302 at 168.611 kb on - strand, within SMa0302 at 168.766 kb on + strand, within SMa0302 at 168.778 kb on - strand, within SMa0302 at 168.779 kb on + strand, within SMa0302 at 168.780 kb on - strand, within SMa0302 at 168.785 kb on - strand, within SMa0302 at 168.848 kb on - strand at 169.032 kb on + strand at 169.032 kb on + strand at 169.032 kb on + strand at 169.272 kb on - strand, within SMa0303 at 169.272 kb on - strand, within SMa0303 at 169.368 kb on - strand, within SMa0303 at 169.368 kb on - strand, within SMa0303 at 169.368 kb on - strand, within SMa0303 at 169.396 kb on - strand, within SMa0303 at 169.506 kb on - strand, within SMa0303 at 169.506 kb on - strand, within SMa0303 at 169.506 kb on - strand, within SMa0303 at 169.507 kb on + strand, within SMa0303 at 169.507 kb on + strand, within SMa0303 at 169.508 kb on - strand, within SMa0303 at 169.508 kb on - strand, within SMa0303 at 169.538 kb on + strand, within SMa0303 at 169.539 kb on - strand, within SMa0303 at 169.726 kb on + strand, within SMa0303 at 169.805 kb on - strand, within SMa0303 at 169.805 kb on - strand, within SMa0303 at 169.866 kb on - strand at 169.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (N) remove 166,496 + SMa0300 0.79 -0.2 166,685 - -0.4 166,685 - -0.5 166,685 - +0.1 166,685 - -0.4 166,789 + SMa0301 0.14 -0.9 166,789 + SMa0301 0.14 +0.7 166,789 + SMa0301 0.14 +0.4 166,790 - SMa0301 0.15 -0.2 166,790 - SMa0301 0.15 +0.6 166,790 - SMa0301 0.15 -0.2 166,925 - SMa0301 0.35 -0.4 166,927 + SMa0301 0.35 -2.4 167,000 + SMa0301 0.46 -0.2 167,001 - SMa0301 0.46 +0.2 167,034 - SMa0301 0.51 -1.5 167,056 + SMa0301 0.54 -0.1 167,057 - SMa0301 0.55 -1.8 167,101 + SMa0301 0.61 -2.7 167,101 + SMa0301 0.61 -0.9 167,102 - SMa0301 0.61 +0.2 167,366 + +0.6 167,366 + -1.0 167,367 - +0.4 167,456 - -1.8 167,456 - +1.1 167,488 + +0.8 167,546 + -3.3 167,734 + SMa0302 0.20 -0.3 167,734 + SMa0302 0.20 -0.4 167,735 - SMa0302 0.20 +0.1 167,776 + SMa0302 0.23 -0.1 167,839 + SMa0302 0.27 +0.2 167,884 + SMa0302 0.30 +0.4 167,884 + SMa0302 0.30 -1.0 168,123 + SMa0302 0.45 +0.3 168,124 - SMa0302 0.45 -0.7 168,133 + SMa0302 0.46 +0.7 168,134 - SMa0302 0.46 +0.9 168,263 + SMa0302 0.55 +0.0 168,303 - SMa0302 0.57 -1.0 168,362 + SMa0302 0.61 -1.2 168,363 - SMa0302 0.61 -2.4 168,363 - SMa0302 0.61 -0.5 168,399 - SMa0302 0.63 +0.1 168,451 + SMa0302 0.67 +1.7 168,451 + SMa0302 0.67 +0.5 168,451 + SMa0302 0.67 -0.2 168,611 - SMa0302 0.77 +0.6 168,766 + SMa0302 0.87 -0.0 168,778 - SMa0302 0.88 -0.4 168,779 + SMa0302 0.88 +1.5 168,780 - SMa0302 0.88 -0.6 168,785 - SMa0302 0.89 +0.2 168,848 - -0.1 169,032 + -0.2 169,032 + +1.0 169,032 + +0.1 169,272 - SMa0303 0.24 -0.8 169,272 - SMa0303 0.24 -0.4 169,368 - SMa0303 0.35 +0.6 169,368 - SMa0303 0.35 -1.8 169,368 - SMa0303 0.35 -0.1 169,396 - SMa0303 0.38 -0.6 169,506 - SMa0303 0.50 +0.2 169,506 - SMa0303 0.50 +5.1 169,506 - SMa0303 0.50 +0.8 169,507 + SMa0303 0.50 -0.5 169,507 + SMa0303 0.50 +1.4 169,508 - SMa0303 0.51 -0.2 169,508 - SMa0303 0.51 +1.1 169,538 + SMa0303 0.54 +0.6 169,539 - SMa0303 0.54 -0.0 169,726 + SMa0303 0.75 +1.1 169,805 - SMa0303 0.84 -0.1 169,805 - SMa0303 0.84 +1.0 169,866 - +0.4 169,866 - +1.9
Or see this region's nucleotide sequence