Experiment: Gelatin (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21702 and SM_b21701 overlap by 123 nucleotides SM_b21701 and SM_b20783 overlap by 73 nucleotides SM_b20783 and SM_b20784 are separated by 172 nucleotides SM_b20784 and SM_b20785 overlap by 20 nucleotides SM_b20785 and SM_b20786 overlap by 4 nucleotides
SM_b21702: SM_b21702 - transposase of insertion sequence ISRm2011-2, orfB N-terminus protein, at 1,576,466 to 1,576,606
_b21702
SM_b21701: SM_b21701 - hypothetical protein, at 1,576,484 to 1,576,621
_b21701
SM_b20783: SM_b20783 - transposase of insertion sequence ISRm2011-2, orfA protein, at 1,576,549 to 1,576,956
_b20783
SM_b20784: SM_b20784 - branched-chain amino acid ABC transporter ATP-binding protein, at 1,577,129 to 1,577,845
_b20784
SM_b20785: SM_b20785 - branched-chain amino acid ABC transporter ATP-binding protein, at 1,577,826 to 1,578,590
_b20785
SM_b20786: SM_b20786 - branched-chain amino acid ABC transporter permease, at 1,578,587 to 1,579,588
_b20786
Position (kb)
1577
1578 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1577.416 kb on + strand, within SM_b20784 at 1577.496 kb on + strand, within SM_b20784 at 1577.496 kb on + strand, within SM_b20784 at 1577.497 kb on - strand, within SM_b20784 at 1577.497 kb on - strand, within SM_b20784 at 1577.674 kb on - strand, within SM_b20784 at 1577.674 kb on - strand, within SM_b20784 at 1577.852 kb on - strand at 1577.863 kb on + strand at 1577.864 kb on - strand at 1578.408 kb on + strand, within SM_b20785
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (N) remove 1,577,416 + SM_b20784 0.40 -0.4 1,577,496 + SM_b20784 0.51 -3.0 1,577,496 + SM_b20784 0.51 -0.4 1,577,497 - SM_b20784 0.51 -0.8 1,577,497 - SM_b20784 0.51 +0.1 1,577,674 - SM_b20784 0.76 -0.2 1,577,674 - SM_b20784 0.76 -3.6 1,577,852 - -0.7 1,577,863 + +0.5 1,577,864 - -0.0 1,578,408 + SM_b20785 0.76 -0.1
Or see this region's nucleotide sequence