Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_77 and Pf1N1B4_78 are separated by 143 nucleotides Pf1N1B4_78 and Pf1N1B4_79 are separated by 179 nucleotides Pf1N1B4_79 and Pf1N1B4_80 are separated by 18 nucleotides
Pf1N1B4_77 - FIG00956064: hypothetical protein, at 81,000 to 81,317
_77
Pf1N1B4_78 - FIG00956937: hypothetical protein, at 81,461 to 81,994
_78
Pf1N1B4_79 - Lactoylglutathione lyase and related lyases, at 82,174 to 82,542
_79
Pf1N1B4_80 - Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77), at 82,561 to 83,844
_80
Position (kb) 82
83 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 81.220 kb on + strand, within Pf1N1B4_77 at 81.289 kb on + strand at 81.290 kb on - strand at 81.290 kb on - strand at 81.315 kb on + strand at 81.316 kb on - strand at 81.334 kb on + strand at 81.335 kb on - strand at 81.335 kb on - strand at 81.403 kb on - strand at 81.403 kb on - strand at 81.426 kb on + strand at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.967 kb on + strand at 81.968 kb on - strand at 82.022 kb on + strand at 82.064 kb on + strand at 82.064 kb on + strand at 82.064 kb on + strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.070 kb on + strand at 82.071 kb on - strand at 82.071 kb on - strand at 82.071 kb on - strand at 82.090 kb on + strand at 82.090 kb on + strand at 82.091 kb on - strand at 82.091 kb on - strand at 82.091 kb on - strand at 82.124 kb on + strand at 82.125 kb on - strand at 82.324 kb on + strand, within Pf1N1B4_79 at 82.324 kb on + strand, within Pf1N1B4_79 at 82.325 kb on - strand, within Pf1N1B4_79 at 82.357 kb on + strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.358 kb on - strand, within Pf1N1B4_79 at 82.492 kb on + strand, within Pf1N1B4_79 at 82.526 kb on + strand at 82.526 kb on + strand at 82.526 kb on + strand at 82.527 kb on - strand at 82.527 kb on - strand at 82.527 kb on - strand at 82.527 kb on - strand at 82.540 kb on + strand at 82.564 kb on + strand at 82.564 kb on + strand at 82.564 kb on + strand at 82.565 kb on - strand at 82.565 kb on - strand at 82.688 kb on + strand at 82.688 kb on + strand at 82.689 kb on - strand at 82.719 kb on - strand, within Pf1N1B4_80 at 82.744 kb on + strand, within Pf1N1B4_80 at 82.744 kb on + strand, within Pf1N1B4_80 at 82.745 kb on - strand, within Pf1N1B4_80 at 83.040 kb on + strand, within Pf1N1B4_80 at 83.041 kb on - strand, within Pf1N1B4_80 at 83.165 kb on + strand, within Pf1N1B4_80 at 83.166 kb on - strand, within Pf1N1B4_80 at 83.364 kb on + strand, within Pf1N1B4_80 at 83.364 kb on + strand at 83.365 kb on - strand, within Pf1N1B4_80 at 83.365 kb on - strand, within Pf1N1B4_80 at 83.366 kb on + strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.423 kb on - strand, within Pf1N1B4_80 at 83.450 kb on + strand, within Pf1N1B4_80
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 81,220 + Pf1N1B4_77 0.69 -0.5 81,289 + -0.1 81,290 - +0.9 81,290 - -0.4 81,315 + -0.4 81,316 - -1.0 81,334 + +0.9 81,335 - +0.8 81,335 - -0.3 81,403 - +0.8 81,403 - +1.2 81,426 + +0.6 81,778 - Pf1N1B4_78 0.59 -0.8 81,778 - Pf1N1B4_78 0.59 +0.9 81,778 - Pf1N1B4_78 0.59 +0.5 81,778 - Pf1N1B4_78 0.59 +0.5 81,967 + +0.6 81,968 - +1.1 82,022 + +1.4 82,064 + -0.2 82,064 + +0.4 82,064 + +0.5 82,065 - +1.5 82,065 - +2.1 82,065 - +0.2 82,065 - +0.4 82,065 - +0.5 82,070 + -1.2 82,071 - -0.6 82,071 - -0.6 82,071 - +1.4 82,090 + +1.2 82,090 + -1.0 82,091 - -1.0 82,091 - +0.3 82,091 - -0.3 82,124 + +2.4 82,125 - +1.4 82,324 + Pf1N1B4_79 0.41 -1.1 82,324 + Pf1N1B4_79 0.41 -1.1 82,325 - Pf1N1B4_79 0.41 -0.1 82,357 + Pf1N1B4_79 0.50 +3.5 82,358 - Pf1N1B4_79 0.50 -0.1 82,358 - Pf1N1B4_79 0.50 -1.0 82,358 - Pf1N1B4_79 0.50 +0.6 82,358 - Pf1N1B4_79 0.50 -2.0 82,358 - Pf1N1B4_79 0.50 +0.9 82,492 + Pf1N1B4_79 0.86 +0.4 82,526 + -0.2 82,526 + -1.6 82,526 + +0.1 82,527 - -0.4 82,527 - -1.2 82,527 - -2.0 82,527 - +0.1 82,540 + -0.6 82,564 + +0.6 82,564 + -0.2 82,564 + +2.0 82,565 - -1.0 82,565 - +0.5 82,688 + +1.1 82,688 + +0.4 82,689 - +0.6 82,719 - Pf1N1B4_80 0.12 -0.1 82,744 + Pf1N1B4_80 0.14 +0.8 82,744 + Pf1N1B4_80 0.14 +0.0 82,745 - Pf1N1B4_80 0.14 +1.7 83,040 + Pf1N1B4_80 0.37 -2.6 83,041 - Pf1N1B4_80 0.37 -1.2 83,165 + Pf1N1B4_80 0.47 +0.8 83,166 - Pf1N1B4_80 0.47 +0.6 83,364 + Pf1N1B4_80 0.63 +1.4 83,364 + +1.4 83,365 - Pf1N1B4_80 0.63 +0.4 83,365 - Pf1N1B4_80 0.63 +0.4 83,366 + Pf1N1B4_80 0.63 -0.6 83,423 - Pf1N1B4_80 0.67 -0.3 83,423 - Pf1N1B4_80 0.67 +0.8 83,450 + Pf1N1B4_80 0.69 +1.2
Or see this region's nucleotide sequence