Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_694 and Pf1N1B4_695 are separated by 218 nucleotides Pf1N1B4_695 and Pf1N1B4_696 overlap by 4 nucleotides
Pf1N1B4_694 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 792,211 to 793,314
_694
Pf1N1B4_695 - Aldehyde dehydrogenase (EC 1.2.1.3), at 793,533 to 794,981
_695
Pf1N1B4_696 - Acetolactate synthase, large subunit (EC 2.2.1.6), at 794,978 to 796,615
_696
Position (kb)
793
794
795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 792.539 kb on + strand, within Pf1N1B4_694 at 792.540 kb on - strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.581 kb on + strand, within Pf1N1B4_694 at 792.582 kb on - strand, within Pf1N1B4_694 at 792.666 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.818 kb on + strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.819 kb on - strand, within Pf1N1B4_694 at 792.855 kb on - strand, within Pf1N1B4_694 at 793.025 kb on + strand, within Pf1N1B4_694 at 793.109 kb on + strand, within Pf1N1B4_694 at 793.110 kb on - strand, within Pf1N1B4_694 at 793.110 kb on - strand, within Pf1N1B4_694 at 793.337 kb on - strand at 793.337 kb on - strand at 793.337 kb on - strand at 793.340 kb on + strand at 793.340 kb on + strand at 793.340 kb on + strand at 793.341 kb on - strand at 793.341 kb on - strand at 793.801 kb on - strand, within Pf1N1B4_695 at 793.914 kb on - strand, within Pf1N1B4_695 at 794.196 kb on + strand, within Pf1N1B4_695 at 794.450 kb on + strand, within Pf1N1B4_695 at 794.457 kb on + strand, within Pf1N1B4_695 at 794.641 kb on + strand at 794.642 kb on - strand, within Pf1N1B4_695 at 794.645 kb on - strand, within Pf1N1B4_695 at 794.645 kb on - strand, within Pf1N1B4_695 at 794.950 kb on + strand at 794.950 kb on + strand at 794.950 kb on + strand at 794.951 kb on - strand at 794.951 kb on - strand at 794.951 kb on - strand at 794.966 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.141 kb on + strand at 795.142 kb on - strand, within Pf1N1B4_696 at 795.142 kb on - strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.183 kb on + strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.184 kb on - strand, within Pf1N1B4_696 at 795.202 kb on - strand, within Pf1N1B4_696 at 795.453 kb on + strand, within Pf1N1B4_696 at 795.701 kb on - strand, within Pf1N1B4_696
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 792,539 + Pf1N1B4_694 0.30 -1.4 792,540 - Pf1N1B4_694 0.30 +0.6 792,581 + Pf1N1B4_694 0.34 +0.6 792,581 + Pf1N1B4_694 0.34 -1.2 792,582 - Pf1N1B4_694 0.34 +1.1 792,666 + Pf1N1B4_694 0.41 -0.0 792,818 + Pf1N1B4_694 0.55 -0.7 792,818 + Pf1N1B4_694 0.55 +0.6 792,818 + Pf1N1B4_694 0.55 +0.1 792,818 + Pf1N1B4_694 0.55 -1.0 792,819 - Pf1N1B4_694 0.55 -0.4 792,819 - Pf1N1B4_694 0.55 +0.1 792,855 - Pf1N1B4_694 0.58 -0.2 793,025 + Pf1N1B4_694 0.74 +0.1 793,109 + Pf1N1B4_694 0.81 +0.6 793,110 - Pf1N1B4_694 0.81 +0.6 793,110 - Pf1N1B4_694 0.81 -1.0 793,337 - -0.4 793,337 - -0.2 793,337 - +1.0 793,340 + +0.1 793,340 + -0.8 793,340 + +0.1 793,341 - +0.2 793,341 - +0.3 793,801 - Pf1N1B4_695 0.18 +0.1 793,914 - Pf1N1B4_695 0.26 -1.2 794,196 + Pf1N1B4_695 0.46 -2.0 794,450 + Pf1N1B4_695 0.63 -2.0 794,457 + Pf1N1B4_695 0.64 -0.4 794,641 + -1.0 794,642 - Pf1N1B4_695 0.77 +0.1 794,645 - Pf1N1B4_695 0.77 -0.0 794,645 - Pf1N1B4_695 0.77 -0.9 794,950 + +0.4 794,950 + +1.7 794,950 + -1.9 794,951 - -2.3 794,951 - +0.6 794,951 - +0.7 794,966 + -1.2 795,141 + -0.4 795,141 + -1.9 795,141 + -1.0 795,141 + +0.6 795,141 + +1.1 795,141 + +0.6 795,141 + +0.1 795,142 - Pf1N1B4_696 0.10 -0.7 795,142 - Pf1N1B4_696 0.10 -0.1 795,183 + Pf1N1B4_696 0.13 +2.2 795,183 + Pf1N1B4_696 0.13 -1.0 795,183 + Pf1N1B4_696 0.13 -0.2 795,183 + Pf1N1B4_696 0.13 -0.9 795,183 + Pf1N1B4_696 0.13 +0.6 795,183 + Pf1N1B4_696 0.13 -1.0 795,184 - Pf1N1B4_696 0.13 -1.2 795,184 - Pf1N1B4_696 0.13 +0.7 795,184 - Pf1N1B4_696 0.13 +0.0 795,184 - Pf1N1B4_696 0.13 -0.2 795,184 - Pf1N1B4_696 0.13 -1.0 795,184 - Pf1N1B4_696 0.13 +0.1 795,202 - Pf1N1B4_696 0.14 -0.8 795,453 + Pf1N1B4_696 0.29 +1.4 795,701 - Pf1N1B4_696 0.44 -0.2
Or see this region's nucleotide sequence