Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5978 and Pf1N1B4_5979 are separated by 120 nucleotides Pf1N1B4_5979 and Pf1N1B4_5980 are separated by 255 nucleotides Pf1N1B4_5980 and Pf1N1B4_5981 are separated by 239 nucleotides
Pf1N1B4_5978 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 426,416 to 427,996
_5978
Pf1N1B4_5979 - Ureidoglycolate/malate/sulfolactate dehydrogenase family (EC 1.1.1.-), at 428,117 to 429,124
_5979
Pf1N1B4_5980 - Organic hydroperoxide resistance protein, at 429,380 to 429,832
_5980
Pf1N1B4_5981 - Organic hydroperoxide resistance transcriptional regulator, at 430,072 to 430,533
_5981
Position (kb)
428
429
430 Strain fitness (log2 ratio)
-2
-1
0
1 at 427.141 kb on - strand, within Pf1N1B4_5978 at 427.442 kb on + strand, within Pf1N1B4_5978 at 427.483 kb on - strand, within Pf1N1B4_5978 at 428.145 kb on + strand at 428.146 kb on - strand at 428.146 kb on - strand at 428.361 kb on + strand, within Pf1N1B4_5979 at 428.362 kb on - strand, within Pf1N1B4_5979 at 428.362 kb on - strand, within Pf1N1B4_5979 at 428.711 kb on - strand, within Pf1N1B4_5979 at 428.711 kb on - strand, within Pf1N1B4_5979 at 429.214 kb on + strand at 429.236 kb on - strand at 429.684 kb on - strand, within Pf1N1B4_5980 at 429.859 kb on - strand at 429.859 kb on - strand at 429.906 kb on + strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand at 429.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 427,141 - Pf1N1B4_5978 0.46 -0.1 427,442 + Pf1N1B4_5978 0.65 -0.8 427,483 - Pf1N1B4_5978 0.67 -0.8 428,145 + +0.0 428,146 - -0.4 428,146 - -0.6 428,361 + Pf1N1B4_5979 0.24 -0.8 428,362 - Pf1N1B4_5979 0.24 -1.4 428,362 - Pf1N1B4_5979 0.24 -0.6 428,711 - Pf1N1B4_5979 0.59 -0.4 428,711 - Pf1N1B4_5979 0.59 -0.0 429,214 + +1.0 429,236 - +1.2 429,684 - Pf1N1B4_5980 0.67 +0.2 429,859 - +0.6 429,859 - -0.5 429,906 + -1.6 429,907 - +0.2 429,907 - +1.1 429,907 - -2.4 429,907 - +0.8 429,907 - -1.4 429,907 - +0.2
Or see this region's nucleotide sequence