Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_535 and Pf1N1B4_536 are separated by 360 nucleotides Pf1N1B4_536 and Pf1N1B4_537 are separated by 209 nucleotides
Pf1N1B4_535 - FIG00953341: hypothetical protein, at 633,973 to 634,809
_535
Pf1N1B4_536 - FIG00953551: hypothetical protein, at 635,170 to 635,349
_536
Pf1N1B4_537 - FIG00959721: hypothetical protein, at 635,559 to 636,275
_537
Position (kb) 633
634
635 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 632.983 kb on + strand at 632.983 kb on + strand at 632.984 kb on - strand at 633.015 kb on + strand at 633.115 kb on + strand at 633.115 kb on + strand at 633.116 kb on - strand at 633.322 kb on - strand at 633.322 kb on - strand at 633.378 kb on - strand at 633.378 kb on - strand at 633.380 kb on - strand at 633.380 kb on - strand at 633.531 kb on + strand at 633.531 kb on + strand at 633.532 kb on - strand at 633.532 kb on - strand at 633.548 kb on + strand at 633.707 kb on - strand at 633.735 kb on + strand at 633.787 kb on - strand at 633.840 kb on + strand at 633.840 kb on + strand at 633.840 kb on + strand at 633.840 kb on + strand at 633.841 kb on - strand at 633.841 kb on - strand at 633.841 kb on - strand at 633.859 kb on + strand at 633.859 kb on + strand at 633.859 kb on + strand at 633.859 kb on + strand at 633.860 kb on - strand at 633.922 kb on + strand at 633.955 kb on + strand at 633.955 kb on + strand at 633.956 kb on - strand at 633.956 kb on - strand at 633.988 kb on - strand at 634.023 kb on - strand at 634.023 kb on - strand at 634.023 kb on - strand at 634.023 kb on - strand at 634.071 kb on + strand at 634.072 kb on - strand, within Pf1N1B4_535 at 634.072 kb on - strand, within Pf1N1B4_535 at 634.072 kb on - strand, within Pf1N1B4_535 at 634.162 kb on - strand, within Pf1N1B4_535 at 634.231 kb on - strand, within Pf1N1B4_535 at 634.231 kb on - strand, within Pf1N1B4_535 at 634.248 kb on + strand, within Pf1N1B4_535 at 634.376 kb on + strand, within Pf1N1B4_535 at 634.376 kb on + strand, within Pf1N1B4_535 at 634.377 kb on - strand at 634.377 kb on - strand, within Pf1N1B4_535 at 634.377 kb on - strand, within Pf1N1B4_535 at 634.377 kb on - strand, within Pf1N1B4_535 at 634.451 kb on + strand, within Pf1N1B4_535 at 634.451 kb on + strand, within Pf1N1B4_535 at 634.451 kb on + strand, within Pf1N1B4_535 at 634.452 kb on - strand, within Pf1N1B4_535 at 634.470 kb on - strand at 634.704 kb on - strand, within Pf1N1B4_535 at 634.749 kb on - strand at 634.751 kb on - strand at 634.841 kb on + strand at 635.052 kb on - strand at 635.101 kb on - strand at 635.128 kb on - strand at 635.153 kb on - strand at 635.177 kb on + strand at 635.347 kb on + strand at 635.361 kb on + strand at 635.500 kb on + strand at 635.500 kb on + strand at 635.518 kb on - strand at 635.760 kb on + strand at 635.760 kb on + strand, within Pf1N1B4_537 at 635.760 kb on + strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537 at 635.761 kb on - strand, within Pf1N1B4_537
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 632,983 + -1.3 632,983 + -0.9 632,984 - -2.1 633,015 + -0.7 633,115 + +0.1 633,115 + -1.7 633,116 - -0.1 633,322 - +1.5 633,322 - -0.1 633,378 - -0.4 633,378 - -0.6 633,380 - -0.1 633,380 - -0.7 633,531 + -1.4 633,531 + +2.1 633,532 - -0.6 633,532 - +0.8 633,548 + -0.3 633,707 - +0.1 633,735 + -1.3 633,787 - +0.5 633,840 + -2.1 633,840 + -1.9 633,840 + -0.4 633,840 + +1.0 633,841 - -0.1 633,841 - +0.2 633,841 - -0.5 633,859 + +1.2 633,859 + -0.7 633,859 + +0.2 633,859 + -0.4 633,860 - -0.5 633,922 + +2.5 633,955 + -0.2 633,955 + -0.5 633,956 - -0.8 633,956 - -0.7 633,988 - +0.5 634,023 - +0.5 634,023 - -2.3 634,023 - -1.3 634,023 - +0.1 634,071 + -0.5 634,072 - Pf1N1B4_535 0.12 -0.2 634,072 - Pf1N1B4_535 0.12 -2.3 634,072 - Pf1N1B4_535 0.12 -0.5 634,162 - Pf1N1B4_535 0.23 -1.3 634,231 - Pf1N1B4_535 0.31 -1.5 634,231 - Pf1N1B4_535 0.31 -1.5 634,248 + Pf1N1B4_535 0.33 -0.5 634,376 + Pf1N1B4_535 0.48 +0.8 634,376 + Pf1N1B4_535 0.48 -1.8 634,377 - -0.1 634,377 - Pf1N1B4_535 0.48 -0.2 634,377 - Pf1N1B4_535 0.48 -2.8 634,377 - Pf1N1B4_535 0.48 +0.7 634,451 + Pf1N1B4_535 0.57 +0.5 634,451 + Pf1N1B4_535 0.57 +1.2 634,451 + Pf1N1B4_535 0.57 -0.3 634,452 - Pf1N1B4_535 0.57 +0.1 634,470 - +1.8 634,704 - Pf1N1B4_535 0.87 -2.3 634,749 - -0.5 634,751 - -0.5 634,841 + +0.3 635,052 - +0.9 635,101 - -1.0 635,128 - -0.7 635,153 - +0.2 635,177 + +0.5 635,347 + +0.5 635,361 + +1.9 635,500 + +0.5 635,500 + -0.1 635,518 - +0.6 635,760 + +0.5 635,760 + Pf1N1B4_537 0.28 -1.1 635,760 + Pf1N1B4_537 0.28 -1.8 635,761 - Pf1N1B4_537 0.28 -0.4 635,761 - Pf1N1B4_537 0.28 +0.5 635,761 - Pf1N1B4_537 0.28 +0.1 635,761 - Pf1N1B4_537 0.28 -1.3 635,761 - Pf1N1B4_537 0.28 -1.2 635,761 - Pf1N1B4_537 0.28 -0.1 635,761 - Pf1N1B4_537 0.28 -0.8 635,761 - Pf1N1B4_537 0.28 +0.2 635,761 - Pf1N1B4_537 0.28 -2.1 635,761 - Pf1N1B4_537 0.28 -0.5 635,761 - Pf1N1B4_537 0.28 -0.5 635,761 - Pf1N1B4_537 0.28 +0.5 635,761 - Pf1N1B4_537 0.28 +1.5
Or see this region's nucleotide sequence