Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5340 and Pf1N1B4_5341 are separated by 357 nucleotides Pf1N1B4_5341 and Pf1N1B4_5342 are separated by 109 nucleotides Pf1N1B4_5342 and Pf1N1B4_5343 are separated by 88 nucleotides Pf1N1B4_5343 and Pf1N1B4_5344 are separated by 158 nucleotides
Pf1N1B4_5340 - hypothetical protein, at 83,949 to 84,311
_5340
Pf1N1B4_5341 - hypothetical protein, at 84,669 to 84,830
_5341
Pf1N1B4_5342 - hypothetical protein, at 84,940 to 85,110
_5342
Pf1N1B4_5343 - hypothetical protein, at 85,199 to 85,933
_5343
Pf1N1B4_5344 - hypothetical protein, at 86,092 to 86,823
_5344
Position (kb)
84
85
86 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 84.023 kb on + strand, within Pf1N1B4_5340 at 84.023 kb on + strand, within Pf1N1B4_5340 at 84.187 kb on + strand, within Pf1N1B4_5340 at 84.187 kb on + strand, within Pf1N1B4_5340 at 84.187 kb on + strand, within Pf1N1B4_5340 at 84.188 kb on - strand, within Pf1N1B4_5340 at 84.188 kb on - strand, within Pf1N1B4_5340 at 84.188 kb on - strand, within Pf1N1B4_5340 at 84.580 kb on + strand at 84.580 kb on + strand at 84.580 kb on + strand at 84.581 kb on - strand at 84.643 kb on - strand at 84.696 kb on + strand, within Pf1N1B4_5341 at 84.907 kb on + strand at 84.907 kb on + strand at 84.907 kb on + strand at 84.908 kb on - strand at 85.007 kb on + strand, within Pf1N1B4_5342 at 85.007 kb on + strand, within Pf1N1B4_5342 at 85.125 kb on - strand at 85.128 kb on + strand at 85.129 kb on + strand at 85.129 kb on + strand at 85.129 kb on + strand at 85.130 kb on - strand at 85.378 kb on + strand, within Pf1N1B4_5343 at 85.378 kb on + strand, within Pf1N1B4_5343 at 85.378 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.384 kb on + strand, within Pf1N1B4_5343 at 85.385 kb on - strand, within Pf1N1B4_5343 at 85.385 kb on - strand, within Pf1N1B4_5343 at 85.385 kb on - strand, within Pf1N1B4_5343 at 85.385 kb on - strand at 85.385 kb on - strand, within Pf1N1B4_5343 at 85.425 kb on + strand, within Pf1N1B4_5343 at 85.425 kb on + strand, within Pf1N1B4_5343 at 85.426 kb on - strand, within Pf1N1B4_5343 at 85.605 kb on + strand, within Pf1N1B4_5343 at 85.605 kb on + strand, within Pf1N1B4_5343 at 85.605 kb on + strand, within Pf1N1B4_5343 at 85.605 kb on + strand, within Pf1N1B4_5343 at 85.605 kb on + strand, within Pf1N1B4_5343 at 85.606 kb on - strand, within Pf1N1B4_5343 at 85.606 kb on - strand, within Pf1N1B4_5343 at 85.641 kb on - strand, within Pf1N1B4_5343 at 85.641 kb on - strand at 85.646 kb on + strand, within Pf1N1B4_5343 at 85.657 kb on + strand, within Pf1N1B4_5343 at 85.657 kb on + strand, within Pf1N1B4_5343 at 85.657 kb on + strand, within Pf1N1B4_5343 at 85.657 kb on + strand, within Pf1N1B4_5343 at 85.657 kb on + strand, within Pf1N1B4_5343 at 85.658 kb on - strand, within Pf1N1B4_5343 at 85.658 kb on - strand, within Pf1N1B4_5343 at 85.658 kb on - strand, within Pf1N1B4_5343 at 85.659 kb on + strand, within Pf1N1B4_5343 at 85.660 kb on - strand, within Pf1N1B4_5343 at 85.660 kb on - strand, within Pf1N1B4_5343 at 85.767 kb on + strand, within Pf1N1B4_5343 at 85.768 kb on - strand, within Pf1N1B4_5343 at 85.768 kb on - strand, within Pf1N1B4_5343 at 85.788 kb on + strand, within Pf1N1B4_5343 at 85.789 kb on - strand, within Pf1N1B4_5343 at 85.789 kb on - strand, within Pf1N1B4_5343 at 85.858 kb on + strand, within Pf1N1B4_5343 at 85.937 kb on + strand at 85.938 kb on - strand at 85.944 kb on + strand at 85.944 kb on + strand at 85.944 kb on + strand at 85.944 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 84,023 + Pf1N1B4_5340 0.20 +0.8 84,023 + Pf1N1B4_5340 0.20 -0.4 84,187 + Pf1N1B4_5340 0.66 +0.2 84,187 + Pf1N1B4_5340 0.66 +0.1 84,187 + Pf1N1B4_5340 0.66 -0.1 84,188 - Pf1N1B4_5340 0.66 +0.3 84,188 - Pf1N1B4_5340 0.66 -1.4 84,188 - Pf1N1B4_5340 0.66 -0.7 84,580 + +1.4 84,580 + +0.3 84,580 + +0.6 84,581 - +0.1 84,643 - +1.8 84,696 + Pf1N1B4_5341 0.17 +0.1 84,907 + +0.0 84,907 + -2.9 84,907 + -0.7 84,908 - +1.6 85,007 + Pf1N1B4_5342 0.39 -0.6 85,007 + Pf1N1B4_5342 0.39 -1.2 85,125 - +1.6 85,128 + +1.1 85,129 + +0.4 85,129 + +0.3 85,129 + -1.0 85,130 - -0.8 85,378 + Pf1N1B4_5343 0.24 +0.3 85,378 + Pf1N1B4_5343 0.24 +0.8 85,378 + Pf1N1B4_5343 0.24 +0.3 85,384 + Pf1N1B4_5343 0.25 +0.7 85,384 + Pf1N1B4_5343 0.25 +0.1 85,384 + Pf1N1B4_5343 0.25 +0.4 85,384 + Pf1N1B4_5343 0.25 -0.9 85,384 + Pf1N1B4_5343 0.25 -0.2 85,384 + Pf1N1B4_5343 0.25 -2.3 85,384 + Pf1N1B4_5343 0.25 -0.9 85,384 + Pf1N1B4_5343 0.25 +0.6 85,384 + Pf1N1B4_5343 0.25 -2.0 85,384 + Pf1N1B4_5343 0.25 +1.1 85,385 - Pf1N1B4_5343 0.25 -1.4 85,385 - Pf1N1B4_5343 0.25 +1.1 85,385 - Pf1N1B4_5343 0.25 -2.0 85,385 - +0.6 85,385 - Pf1N1B4_5343 0.25 +0.3 85,425 + Pf1N1B4_5343 0.31 -0.7 85,425 + Pf1N1B4_5343 0.31 -1.0 85,426 - Pf1N1B4_5343 0.31 -0.1 85,605 + Pf1N1B4_5343 0.55 -0.9 85,605 + Pf1N1B4_5343 0.55 -0.2 85,605 + Pf1N1B4_5343 0.55 -2.3 85,605 + Pf1N1B4_5343 0.55 -0.1 85,605 + Pf1N1B4_5343 0.55 -1.4 85,606 - Pf1N1B4_5343 0.55 +0.3 85,606 - Pf1N1B4_5343 0.55 +0.6 85,641 - Pf1N1B4_5343 0.60 -0.3 85,641 - +1.6 85,646 + Pf1N1B4_5343 0.61 +0.6 85,657 + Pf1N1B4_5343 0.62 +3.4 85,657 + Pf1N1B4_5343 0.62 +0.7 85,657 + Pf1N1B4_5343 0.62 -1.4 85,657 + Pf1N1B4_5343 0.62 +1.1 85,657 + Pf1N1B4_5343 0.62 -0.8 85,658 - Pf1N1B4_5343 0.62 -0.1 85,658 - Pf1N1B4_5343 0.62 -0.7 85,658 - Pf1N1B4_5343 0.62 -0.4 85,659 + Pf1N1B4_5343 0.63 +0.3 85,660 - Pf1N1B4_5343 0.63 -0.0 85,660 - Pf1N1B4_5343 0.63 -0.3 85,767 + Pf1N1B4_5343 0.77 -1.0 85,768 - Pf1N1B4_5343 0.77 +0.2 85,768 - Pf1N1B4_5343 0.77 +0.1 85,788 + Pf1N1B4_5343 0.80 -1.0 85,789 - Pf1N1B4_5343 0.80 -0.2 85,789 - Pf1N1B4_5343 0.80 -1.3 85,858 + Pf1N1B4_5343 0.90 -0.2 85,937 + -2.5 85,938 - -0.4 85,944 + +0.3 85,944 + +0.6 85,944 + -2.0 85,944 + -1.0
Or see this region's nucleotide sequence