Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_528 and Pf1N1B4_529 are separated by 82 nucleotides Pf1N1B4_529 and Pf1N1B4_530 are separated by 78 nucleotides Pf1N1B4_530 and Pf1N1B4_531 are separated by 186 nucleotides Pf1N1B4_531 and Pf1N1B4_532 are separated by 140 nucleotides
Pf1N1B4_528 - sodium-solute symporter, putative, at 627,690 to 629,069
_528
Pf1N1B4_529 - SoxH protein, homolog, at 629,152 to 629,877
_529
Pf1N1B4_530 - hypothetical protein, at 629,956 to 630,180
_530
Pf1N1B4_531 - hypothetical protein, at 630,367 to 630,495
_531
Pf1N1B4_532 - Transcriptional regulators, LysR family, at 630,636 to 631,529
_532
Position (kb)
629
630 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 628.176 kb on + strand, within Pf1N1B4_528 at 628.177 kb on - strand, within Pf1N1B4_528 at 628.278 kb on + strand, within Pf1N1B4_528 at 628.368 kb on + strand, within Pf1N1B4_528 at 628.368 kb on + strand, within Pf1N1B4_528 at 628.369 kb on - strand, within Pf1N1B4_528 at 628.369 kb on - strand, within Pf1N1B4_528 at 628.380 kb on + strand at 628.380 kb on + strand, within Pf1N1B4_528 at 628.380 kb on + strand at 628.464 kb on - strand, within Pf1N1B4_528 at 628.479 kb on + strand, within Pf1N1B4_528 at 628.479 kb on + strand, within Pf1N1B4_528 at 628.479 kb on + strand, within Pf1N1B4_528 at 628.601 kb on + strand, within Pf1N1B4_528 at 628.601 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.828 kb on - strand, within Pf1N1B4_528 at 628.983 kb on + strand at 628.983 kb on + strand at 628.983 kb on + strand at 628.983 kb on + strand at 628.984 kb on - strand at 628.984 kb on - strand at 628.984 kb on - strand at 628.986 kb on + strand at 628.987 kb on - strand at 628.987 kb on - strand at 629.067 kb on + strand at 629.073 kb on + strand at 629.074 kb on - strand at 629.152 kb on - strand at 629.227 kb on + strand, within Pf1N1B4_529 at 629.227 kb on + strand at 629.227 kb on + strand, within Pf1N1B4_529 at 629.227 kb on + strand, within Pf1N1B4_529 at 629.227 kb on + strand, within Pf1N1B4_529 at 629.228 kb on - strand at 629.228 kb on - strand at 629.228 kb on - strand, within Pf1N1B4_529 at 629.228 kb on - strand, within Pf1N1B4_529 at 629.232 kb on + strand, within Pf1N1B4_529 at 629.232 kb on + strand, within Pf1N1B4_529 at 629.233 kb on - strand, within Pf1N1B4_529 at 629.243 kb on - strand, within Pf1N1B4_529 at 629.704 kb on + strand at 629.705 kb on - strand, within Pf1N1B4_529 at 629.705 kb on - strand, within Pf1N1B4_529 at 629.732 kb on - strand, within Pf1N1B4_529 at 629.732 kb on - strand, within Pf1N1B4_529 at 629.753 kb on - strand, within Pf1N1B4_529 at 629.887 kb on + strand at 629.887 kb on + strand at 629.888 kb on - strand at 629.917 kb on - strand at 629.917 kb on - strand at 629.924 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.975 kb on - strand at 629.975 kb on - strand at 629.975 kb on - strand at 629.975 kb on - strand at 629.975 kb on - strand at 630.046 kb on - strand, within Pf1N1B4_530 at 630.195 kb on + strand at 630.557 kb on + strand at 630.558 kb on - strand at 630.558 kb on - strand at 630.570 kb on + strand at 630.607 kb on + strand at 630.607 kb on + strand at 630.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 628,176 + Pf1N1B4_528 0.35 -0.9 628,177 - Pf1N1B4_528 0.35 -1.2 628,278 + Pf1N1B4_528 0.43 -1.5 628,368 + Pf1N1B4_528 0.49 +1.1 628,368 + Pf1N1B4_528 0.49 +0.2 628,369 - Pf1N1B4_528 0.49 +0.1 628,369 - Pf1N1B4_528 0.49 -1.8 628,380 + -0.1 628,380 + Pf1N1B4_528 0.50 -1.1 628,380 + +0.5 628,464 - Pf1N1B4_528 0.56 +0.8 628,479 + Pf1N1B4_528 0.57 -0.3 628,479 + Pf1N1B4_528 0.57 -2.5 628,479 + Pf1N1B4_528 0.57 +2.9 628,601 + Pf1N1B4_528 0.66 +0.7 628,601 + Pf1N1B4_528 0.66 +0.7 628,827 + Pf1N1B4_528 0.82 -1.1 628,827 + Pf1N1B4_528 0.82 -0.5 628,827 + Pf1N1B4_528 0.82 +1.1 628,827 + Pf1N1B4_528 0.82 +0.5 628,828 - Pf1N1B4_528 0.82 +0.5 628,983 + +0.5 628,983 + -1.8 628,983 + -1.3 628,983 + -1.7 628,984 - +0.5 628,984 - -1.3 628,984 - +0.1 628,986 + -0.3 628,987 - -1.8 628,987 - -0.1 629,067 + -0.6 629,073 + +0.7 629,074 - -1.8 629,152 - -0.0 629,227 + Pf1N1B4_529 0.10 -0.7 629,227 + +2.1 629,227 + Pf1N1B4_529 0.10 -0.2 629,227 + Pf1N1B4_529 0.10 +0.3 629,227 + Pf1N1B4_529 0.10 -2.7 629,228 - -0.5 629,228 - +3.1 629,228 - Pf1N1B4_529 0.10 -1.3 629,228 - Pf1N1B4_529 0.10 +0.7 629,232 + Pf1N1B4_529 0.11 +0.3 629,232 + Pf1N1B4_529 0.11 -0.5 629,233 - Pf1N1B4_529 0.11 -2.5 629,243 - Pf1N1B4_529 0.13 -1.8 629,704 + +0.9 629,705 - Pf1N1B4_529 0.76 -0.8 629,705 - Pf1N1B4_529 0.76 -0.1 629,732 - Pf1N1B4_529 0.80 -1.6 629,732 - Pf1N1B4_529 0.80 -1.3 629,753 - Pf1N1B4_529 0.83 -0.2 629,887 + -0.7 629,887 + +0.9 629,888 - +0.5 629,917 - +0.5 629,917 - -0.7 629,924 + +1.8 629,974 + -0.3 629,974 + +0.3 629,974 + +0.5 629,974 + -0.5 629,974 + -1.8 629,975 - +0.5 629,975 - +0.8 629,975 - -0.3 629,975 - -0.5 629,975 - +1.2 630,046 - Pf1N1B4_530 0.40 -0.7 630,195 + -0.5 630,557 + -0.5 630,558 - -1.1 630,558 - -1.1 630,570 + -2.3 630,607 + -0.5 630,607 + +0.8 630,674 - +0.4
Or see this region's nucleotide sequence