Experiment: methylglyoxal 0.004 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5019 and Pf1N1B4_5020 are separated by 212 nucleotides Pf1N1B4_5020 and Pf1N1B4_5021 are separated by 22 nucleotides Pf1N1B4_5021 and Pf1N1B4_5022 are separated by 127 nucleotides Pf1N1B4_5022 and Pf1N1B4_5023 overlap by 11 nucleotides Pf1N1B4_5023 and Pf1N1B4_5024 are separated by 60 nucleotides
Pf1N1B4_5019 - FIG00958773: hypothetical protein, at 5,568,806 to 5,569,162
_5019
Pf1N1B4_5020 - FIG00954920: hypothetical protein, at 5,569,375 to 5,569,677
_5020
Pf1N1B4_5021 - FIG00958580: hypothetical protein, at 5,569,700 to 5,569,876
_5021
Pf1N1B4_5022 - hypothetical protein, at 5,570,004 to 5,570,714
_5022
Pf1N1B4_5023 - Alkylated DNA repair protein AlkB, at 5,570,704 to 5,571,384
_5023
Pf1N1B4_5024 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 5,571,445 to 5,572,545
_5024
Position (kb) 5570
5571 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5569.064 kb on + strand, within Pf1N1B4_5019 at 5569.229 kb on - strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.237 kb on + strand at 5569.250 kb on - strand at 5569.699 kb on + strand at 5569.699 kb on + strand at 5569.700 kb on - strand at 5569.700 kb on - strand at 5569.725 kb on - strand at 5569.725 kb on - strand, within Pf1N1B4_5021 at 5570.009 kb on + strand at 5570.122 kb on + strand at 5570.122 kb on + strand, within Pf1N1B4_5022 at 5570.134 kb on - strand, within Pf1N1B4_5022 at 5570.199 kb on + strand, within Pf1N1B4_5022 at 5570.199 kb on + strand, within Pf1N1B4_5022 at 5570.200 kb on - strand, within Pf1N1B4_5022 at 5570.200 kb on - strand, within Pf1N1B4_5022 at 5570.206 kb on - strand at 5570.206 kb on - strand, within Pf1N1B4_5022 at 5570.289 kb on + strand, within Pf1N1B4_5022 at 5570.289 kb on + strand at 5570.394 kb on + strand, within Pf1N1B4_5022 at 5570.409 kb on + strand, within Pf1N1B4_5022 at 5570.410 kb on - strand, within Pf1N1B4_5022 at 5570.800 kb on + strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.801 kb on - strand, within Pf1N1B4_5023 at 5570.907 kb on + strand, within Pf1N1B4_5023 at 5570.908 kb on - strand at 5570.908 kb on - strand, within Pf1N1B4_5023 at 5570.974 kb on + strand, within Pf1N1B4_5023 at 5571.108 kb on + strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.109 kb on - strand, within Pf1N1B4_5023 at 5571.418 kb on - strand at 5571.418 kb on - strand at 5571.437 kb on + strand at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.566 kb on - strand, within Pf1N1B4_5024 at 5571.589 kb on + strand, within Pf1N1B4_5024 at 5571.590 kb on - strand at 5571.590 kb on - strand, within Pf1N1B4_5024
Per-strain Table
Position Strand Gene LocusTag Fraction methylglyoxal 0.004 vol% remove 5,569,064 + Pf1N1B4_5019 0.72 +1.3 5,569,229 - +0.0 5,569,237 + -0.6 5,569,237 + +2.1 5,569,237 + +0.0 5,569,250 - +0.0 5,569,699 + +0.2 5,569,699 + +0.5 5,569,700 - -0.6 5,569,700 - -0.6 5,569,725 - +0.6 5,569,725 - Pf1N1B4_5021 0.14 +0.7 5,570,009 + -1.0 5,570,122 + +0.0 5,570,122 + Pf1N1B4_5022 0.17 -1.2 5,570,134 - Pf1N1B4_5022 0.18 -2.5 5,570,199 + Pf1N1B4_5022 0.27 -1.0 5,570,199 + Pf1N1B4_5022 0.27 +0.0 5,570,200 - Pf1N1B4_5022 0.28 -2.3 5,570,200 - Pf1N1B4_5022 0.28 -1.6 5,570,206 - -0.6 5,570,206 - Pf1N1B4_5022 0.28 +0.6 5,570,289 + Pf1N1B4_5022 0.40 -2.8 5,570,289 + +0.3 5,570,394 + Pf1N1B4_5022 0.55 -3.0 5,570,409 + Pf1N1B4_5022 0.57 -1.0 5,570,410 - Pf1N1B4_5022 0.57 +0.2 5,570,800 + Pf1N1B4_5023 0.14 +0.0 5,570,801 - Pf1N1B4_5023 0.14 +0.0 5,570,801 - Pf1N1B4_5023 0.14 +0.3 5,570,801 - Pf1N1B4_5023 0.14 +0.0 5,570,907 + Pf1N1B4_5023 0.30 -0.2 5,570,908 - +2.0 5,570,908 - Pf1N1B4_5023 0.30 +0.6 5,570,974 + Pf1N1B4_5023 0.40 +0.8 5,571,108 + Pf1N1B4_5023 0.59 -1.6 5,571,109 - Pf1N1B4_5023 0.59 -1.6 5,571,109 - +1.3 5,571,109 - Pf1N1B4_5023 0.59 -0.1 5,571,109 - Pf1N1B4_5023 0.59 +0.2 5,571,418 - -0.6 5,571,418 - +0.3 5,571,437 + -0.4 5,571,566 - Pf1N1B4_5024 0.11 -0.3 5,571,566 - Pf1N1B4_5024 0.11 +0.7 5,571,566 - Pf1N1B4_5024 0.11 +1.3 5,571,566 - Pf1N1B4_5024 0.11 -1.7 5,571,589 + Pf1N1B4_5024 0.13 +0.4 5,571,590 - +0.0 5,571,590 - Pf1N1B4_5024 0.13 -2.0
Or see this region's nucleotide sequence